Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5170 and Pf1N1B4_5171 are separated by 6 nucleotides Pf1N1B4_5171 and Pf1N1B4_5172 are separated by 107 nucleotides Pf1N1B4_5172 and Pf1N1B4_5173 are separated by 5 nucleotides Pf1N1B4_5173 and Pf1N1B4_507 are separated by 395 nucleotides Pf1N1B4_507 and Pf1N1B4_508 are separated by 193 nucleotides
Pf1N1B4_5170 - tRNA-Gly-GCC, at 597,787 to 597,859
_5170
Pf1N1B4_5171 - tRNA-Glu-TTC, at 597,866 to 597,938
_5171
Pf1N1B4_5172 - tRNA-Gly-GCC, at 598,046 to 598,118
_5172
Pf1N1B4_5173 - tRNA-Glu-TTC, at 598,124 to 598,195
_5173
Pf1N1B4_507 - FIG00956448: hypothetical protein, at 598,591 to 599,349
_507
Pf1N1B4_508 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 599,543 to 601,495
_508
Position (kb) 598
599
600 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 597.682 kb on + strand at 597.711 kb on - strand at 597.735 kb on + strand at 597.995 kb on - strand at 598.202 kb on - strand at 598.202 kb on - strand at 598.298 kb on - strand at 598.374 kb on + strand at 598.374 kb on + strand at 598.374 kb on + strand at 598.374 kb on + strand at 598.376 kb on + strand at 598.376 kb on + strand at 598.399 kb on + strand at 598.401 kb on + strand at 598.401 kb on + strand at 598.401 kb on + strand at 598.402 kb on - strand at 598.402 kb on - strand at 598.402 kb on - strand at 598.440 kb on + strand at 598.441 kb on - strand at 598.441 kb on - strand at 598.444 kb on + strand at 598.444 kb on + strand at 598.444 kb on + strand at 598.444 kb on + strand at 598.444 kb on + strand at 598.444 kb on + strand at 598.444 kb on + strand at 598.444 kb on + strand at 598.444 kb on + strand at 598.445 kb on - strand at 598.445 kb on - strand at 598.445 kb on - strand at 598.445 kb on - strand at 598.445 kb on - strand at 598.445 kb on - strand at 598.445 kb on - strand at 598.448 kb on + strand at 598.448 kb on + strand at 598.448 kb on + strand at 598.448 kb on + strand at 598.448 kb on + strand at 598.448 kb on + strand at 598.448 kb on + strand at 598.449 kb on - strand at 598.449 kb on - strand at 598.449 kb on - strand at 598.449 kb on - strand at 598.452 kb on + strand at 598.452 kb on + strand at 598.452 kb on + strand at 598.452 kb on + strand at 598.453 kb on - strand at 598.453 kb on - strand at 598.453 kb on - strand at 598.472 kb on - strand at 598.472 kb on - strand at 598.473 kb on + strand at 598.473 kb on + strand at 598.473 kb on + strand at 598.473 kb on + strand at 598.473 kb on + strand at 598.473 kb on + strand at 598.473 kb on + strand at 598.474 kb on - strand at 598.474 kb on - strand at 598.532 kb on + strand at 598.532 kb on + strand at 598.532 kb on + strand at 598.532 kb on + strand at 598.532 kb on + strand at 598.533 kb on - strand at 598.533 kb on - strand at 598.533 kb on - strand at 598.533 kb on - strand at 598.535 kb on + strand at 598.536 kb on - strand at 598.536 kb on - strand at 598.562 kb on + strand at 598.562 kb on + strand at 598.563 kb on - strand at 598.563 kb on - strand at 598.604 kb on + strand at 598.605 kb on - strand at 598.626 kb on - strand at 598.642 kb on + strand at 598.642 kb on + strand at 598.642 kb on + strand at 598.643 kb on - strand at 598.668 kb on + strand, within Pf1N1B4_507 at 598.668 kb on + strand, within Pf1N1B4_507 at 598.669 kb on - strand, within Pf1N1B4_507 at 599.158 kb on + strand, within Pf1N1B4_507 at 599.207 kb on - strand, within Pf1N1B4_507 at 599.207 kb on - strand at 599.341 kb on + strand at 599.341 kb on + strand at 599.341 kb on + strand at 599.342 kb on - strand at 599.342 kb on - strand at 599.545 kb on - strand at 599.545 kb on - strand at 599.545 kb on - strand at 599.545 kb on - strand at 599.545 kb on - strand at 599.545 kb on - strand at 599.545 kb on - strand at 599.602 kb on + strand at 599.603 kb on - strand at 600.047 kb on + strand, within Pf1N1B4_508 at 600.217 kb on + strand, within Pf1N1B4_508
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 597,682 + +0.6 597,711 - -1.4 597,735 + +2.0 597,995 - -0.4 598,202 - +1.1 598,202 - -2.0 598,298 - -0.0 598,374 + -0.4 598,374 + -0.7 598,374 + -0.3 598,374 + -0.9 598,376 + -0.2 598,376 + +0.6 598,399 + -0.9 598,401 + -0.3 598,401 + -0.6 598,401 + -0.4 598,402 - -0.2 598,402 - -0.9 598,402 - +1.9 598,440 + -0.0 598,441 - +0.4 598,441 - +1.0 598,444 + +1.6 598,444 + +1.2 598,444 + -0.3 598,444 + -0.4 598,444 + +2.2 598,444 + -1.2 598,444 + -0.6 598,444 + -0.6 598,444 + -0.6 598,445 - -0.1 598,445 - -0.7 598,445 - -0.3 598,445 - -1.0 598,445 - -3.1 598,445 - -0.0 598,445 - -0.0 598,448 + -0.4 598,448 + -0.4 598,448 + +1.2 598,448 + -0.8 598,448 + +1.0 598,448 + -0.9 598,448 + -1.3 598,449 - -0.4 598,449 - -1.0 598,449 - -0.6 598,449 - +0.2 598,452 + -0.8 598,452 + -1.2 598,452 + -0.0 598,452 + +0.3 598,453 - +2.1 598,453 - -0.4 598,453 - -0.4 598,472 - +0.2 598,472 - -0.8 598,473 + +0.6 598,473 + -0.8 598,473 + +0.2 598,473 + -0.9 598,473 + -1.2 598,473 + -1.0 598,473 + +0.2 598,474 - -2.1 598,474 - +0.2 598,532 + +0.2 598,532 + +0.4 598,532 + -1.6 598,532 + +0.6 598,532 + +0.3 598,533 - -0.0 598,533 - +0.4 598,533 - +0.3 598,533 - -1.4 598,535 + -1.8 598,536 - -0.9 598,536 - -0.4 598,562 + -0.4 598,562 + -1.8 598,563 - +1.1 598,563 - -1.0 598,604 + -1.0 598,605 - -0.6 598,626 - -0.8 598,642 + +0.1 598,642 + +0.6 598,642 + -0.2 598,643 - +0.1 598,668 + Pf1N1B4_507 0.10 +0.3 598,668 + Pf1N1B4_507 0.10 +2.1 598,669 - Pf1N1B4_507 0.10 +0.8 599,158 + Pf1N1B4_507 0.75 +1.1 599,207 - Pf1N1B4_507 0.81 +0.1 599,207 - +1.0 599,341 + -0.0 599,341 + -0.0 599,341 + -0.8 599,342 - -0.2 599,342 - -2.3 599,545 - +0.7 599,545 - -2.5 599,545 - -0.9 599,545 - +0.2 599,545 - +3.3 599,545 - +0.8 599,545 - +0.7 599,602 + -1.0 599,603 - -0.7 600,047 + Pf1N1B4_508 0.26 -0.2 600,217 + Pf1N1B4_508 0.35 +0.9
Or see this region's nucleotide sequence