Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5053 and Pf1N1B4_5054 overlap by 4 nucleotides Pf1N1B4_5054 and Pf1N1B4_5055 overlap by 24 nucleotides Pf1N1B4_5055 and Pf1N1B4_5056 are separated by 309 nucleotides
Pf1N1B4_5053 - Sensory box histidine kinase/response regulator, at 5,596,328 to 5,598,655
_5053
Pf1N1B4_5054 - Two-component system regulatory protein, at 5,598,652 to 5,599,116
_5054
Pf1N1B4_5055 - (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-), at 5,599,093 to 5,600,010
_5055
Pf1N1B4_5056 - Uncharacterized glutathione S-transferase-like protein, at 5,600,320 to 5,601,006
_5056
Position (kb)
5599
5600
5601 Strain fitness (log2 ratio)
-1
0
1
2 at 5598.746 kb on + strand, within Pf1N1B4_5054 at 5598.747 kb on - strand, within Pf1N1B4_5054 at 5598.747 kb on - strand, within Pf1N1B4_5054 at 5599.054 kb on - strand, within Pf1N1B4_5054 at 5599.054 kb on - strand, within Pf1N1B4_5054 at 5599.111 kb on - strand at 5599.111 kb on - strand at 5599.178 kb on - strand at 5599.337 kb on + strand, within Pf1N1B4_5055 at 5599.337 kb on + strand, within Pf1N1B4_5055 at 5599.337 kb on + strand at 5599.338 kb on - strand, within Pf1N1B4_5055 at 5599.401 kb on - strand, within Pf1N1B4_5055 at 5599.401 kb on - strand at 5599.599 kb on - strand, within Pf1N1B4_5055 at 5599.756 kb on + strand, within Pf1N1B4_5055 at 5599.757 kb on - strand, within Pf1N1B4_5055 at 5599.843 kb on + strand, within Pf1N1B4_5055 at 5599.844 kb on - strand, within Pf1N1B4_5055 at 5599.920 kb on + strand at 5599.921 kb on - strand at 5599.949 kb on + strand at 5599.961 kb on + strand at 5600.195 kb on + strand at 5600.195 kb on + strand at 5600.242 kb on + strand at 5600.335 kb on + strand at 5600.338 kb on + strand at 5600.339 kb on - strand at 5600.369 kb on + strand at 5600.539 kb on + strand, within Pf1N1B4_5056 at 5600.635 kb on + strand, within Pf1N1B4_5056 at 5600.639 kb on + strand, within Pf1N1B4_5056 at 5600.639 kb on + strand, within Pf1N1B4_5056 at 5600.640 kb on - strand at 5600.640 kb on - strand, within Pf1N1B4_5056 at 5600.728 kb on + strand, within Pf1N1B4_5056 at 5600.821 kb on + strand, within Pf1N1B4_5056 at 5600.821 kb on + strand, within Pf1N1B4_5056 at 5600.826 kb on + strand at 5600.872 kb on + strand, within Pf1N1B4_5056
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 5,598,746 + Pf1N1B4_5054 0.20 -0.7 5,598,747 - Pf1N1B4_5054 0.20 +0.7 5,598,747 - Pf1N1B4_5054 0.20 -1.6 5,599,054 - Pf1N1B4_5054 0.86 +0.6 5,599,054 - Pf1N1B4_5054 0.86 +0.9 5,599,111 - +1.2 5,599,111 - -0.0 5,599,178 - +1.0 5,599,337 + Pf1N1B4_5055 0.27 +1.8 5,599,337 + Pf1N1B4_5055 0.27 +0.6 5,599,337 + +1.1 5,599,338 - Pf1N1B4_5055 0.27 +1.2 5,599,401 - Pf1N1B4_5055 0.34 +1.2 5,599,401 - +1.6 5,599,599 - Pf1N1B4_5055 0.55 +0.3 5,599,756 + Pf1N1B4_5055 0.72 +0.8 5,599,757 - Pf1N1B4_5055 0.72 +1.4 5,599,843 + Pf1N1B4_5055 0.82 +1.4 5,599,844 - Pf1N1B4_5055 0.82 +0.4 5,599,920 + -0.2 5,599,921 - +1.3 5,599,949 + +0.6 5,599,961 + +1.9 5,600,195 + -0.0 5,600,195 + +1.0 5,600,242 + -0.1 5,600,335 + -0.2 5,600,338 + +0.9 5,600,339 - +0.6 5,600,369 + +1.5 5,600,539 + Pf1N1B4_5056 0.32 +0.9 5,600,635 + Pf1N1B4_5056 0.46 +0.4 5,600,639 + Pf1N1B4_5056 0.46 -0.5 5,600,639 + Pf1N1B4_5056 0.46 +0.3 5,600,640 - -0.0 5,600,640 - Pf1N1B4_5056 0.47 -1.2 5,600,728 + Pf1N1B4_5056 0.59 -0.4 5,600,821 + Pf1N1B4_5056 0.73 -0.9 5,600,821 + Pf1N1B4_5056 0.73 +1.4 5,600,826 + +0.2 5,600,872 + Pf1N1B4_5056 0.80 -1.3
Or see this region's nucleotide sequence