Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4834 and Pf1N1B4_4835 are separated by 387 nucleotides Pf1N1B4_4835 and Pf1N1B4_4836 are separated by 15 nucleotides Pf1N1B4_4836 and Pf1N1B4_4837 are separated by 27 nucleotides
Pf1N1B4_4834 - Sensor histidine kinase, at 5,338,338 to 5,339,699
_4834
Pf1N1B4_4835 - Carbon starvation protein A, at 5,340,087 to 5,342,144
_4835
Pf1N1B4_4836 - COG2879, Hypothetical small protein yjiX, at 5,342,160 to 5,342,357
_4836
Pf1N1B4_4837 - hypothetical protein, at 5,342,385 to 5,342,555
_4837
Position (kb)
5340
5341
5342
5343 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5339.164 kb on + strand, within Pf1N1B4_4834 at 5339.164 kb on + strand, within Pf1N1B4_4834 at 5339.164 kb on + strand, within Pf1N1B4_4834 at 5339.212 kb on + strand, within Pf1N1B4_4834 at 5339.213 kb on - strand, within Pf1N1B4_4834 at 5339.213 kb on - strand, within Pf1N1B4_4834 at 5339.213 kb on - strand, within Pf1N1B4_4834 at 5339.213 kb on - strand at 5339.213 kb on - strand, within Pf1N1B4_4834 at 5339.347 kb on + strand, within Pf1N1B4_4834 at 5339.348 kb on - strand, within Pf1N1B4_4834 at 5339.348 kb on - strand, within Pf1N1B4_4834 at 5339.404 kb on + strand, within Pf1N1B4_4834 at 5339.416 kb on + strand, within Pf1N1B4_4834 at 5339.497 kb on + strand, within Pf1N1B4_4834 at 5339.498 kb on - strand, within Pf1N1B4_4834 at 5339.534 kb on - strand, within Pf1N1B4_4834 at 5339.744 kb on + strand at 5339.744 kb on + strand at 5339.744 kb on + strand at 5339.745 kb on - strand at 5339.755 kb on + strand at 5339.875 kb on + strand at 5339.947 kb on + strand at 5339.948 kb on - strand at 5339.948 kb on - strand at 5339.948 kb on - strand at 5339.948 kb on - strand at 5339.953 kb on + strand at 5339.954 kb on - strand at 5339.957 kb on + strand at 5339.968 kb on + strand at 5339.969 kb on - strand at 5339.972 kb on + strand at 5339.973 kb on - strand at 5339.987 kb on - strand at 5340.041 kb on - strand at 5340.041 kb on - strand at 5340.041 kb on - strand at 5340.041 kb on - strand at 5340.041 kb on - strand at 5340.043 kb on - strand at 5340.045 kb on + strand at 5340.046 kb on - strand at 5340.219 kb on + strand at 5340.219 kb on + strand at 5340.220 kb on - strand at 5340.220 kb on - strand at 5340.220 kb on - strand at 5340.220 kb on - strand at 5340.238 kb on - strand at 5340.241 kb on - strand at 5340.324 kb on + strand, within Pf1N1B4_4835 at 5340.712 kb on - strand at 5341.920 kb on + strand, within Pf1N1B4_4835 at 5341.993 kb on - strand at 5341.993 kb on - strand at 5341.993 kb on - strand at 5342.001 kb on + strand at 5342.066 kb on + strand at 5342.066 kb on + strand at 5342.066 kb on + strand at 5342.066 kb on + strand at 5342.103 kb on - strand at 5342.191 kb on - strand at 5342.242 kb on - strand, within Pf1N1B4_4836 at 5342.242 kb on - strand, within Pf1N1B4_4836 at 5342.242 kb on - strand, within Pf1N1B4_4836 at 5342.252 kb on + strand, within Pf1N1B4_4836 at 5342.252 kb on + strand, within Pf1N1B4_4836 at 5342.253 kb on - strand, within Pf1N1B4_4836 at 5342.253 kb on - strand, within Pf1N1B4_4836 at 5342.369 kb on + strand at 5342.461 kb on - strand, within Pf1N1B4_4837 at 5342.461 kb on - strand, within Pf1N1B4_4837 at 5342.461 kb on - strand, within Pf1N1B4_4837 at 5342.536 kb on + strand, within Pf1N1B4_4837 at 5342.537 kb on - strand, within Pf1N1B4_4837 at 5342.537 kb on - strand, within Pf1N1B4_4837 at 5342.537 kb on - strand, within Pf1N1B4_4837 at 5342.611 kb on - strand at 5342.716 kb on - strand at 5342.716 kb on - strand at 5342.748 kb on - strand at 5342.789 kb on - strand at 5343.022 kb on - strand at 5343.022 kb on - strand at 5343.023 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 5,339,164 + Pf1N1B4_4834 0.61 +0.2 5,339,164 + Pf1N1B4_4834 0.61 -2.3 5,339,164 + Pf1N1B4_4834 0.61 -4.2 5,339,212 + Pf1N1B4_4834 0.64 -2.0 5,339,213 - Pf1N1B4_4834 0.64 -2.2 5,339,213 - Pf1N1B4_4834 0.64 -1.0 5,339,213 - Pf1N1B4_4834 0.64 +0.5 5,339,213 - -0.3 5,339,213 - Pf1N1B4_4834 0.64 -1.5 5,339,347 + Pf1N1B4_4834 0.74 -2.5 5,339,348 - Pf1N1B4_4834 0.74 -3.0 5,339,348 - Pf1N1B4_4834 0.74 -3.0 5,339,404 + Pf1N1B4_4834 0.78 -2.7 5,339,416 + Pf1N1B4_4834 0.79 +2.9 5,339,497 + Pf1N1B4_4834 0.85 +1.7 5,339,498 - Pf1N1B4_4834 0.85 -0.6 5,339,534 - Pf1N1B4_4834 0.88 +0.5 5,339,744 + +0.3 5,339,744 + -0.8 5,339,744 + +2.1 5,339,745 - +0.3 5,339,755 + -3.2 5,339,875 + -0.3 5,339,947 + -1.1 5,339,948 - -1.5 5,339,948 - -2.9 5,339,948 - -2.1 5,339,948 - -1.5 5,339,953 + -3.2 5,339,954 - -2.6 5,339,957 + -0.8 5,339,968 + -2.3 5,339,969 - -2.5 5,339,972 + -2.5 5,339,973 - -2.5 5,339,987 - -2.9 5,340,041 - -1.3 5,340,041 - -2.7 5,340,041 - -0.8 5,340,041 - -0.5 5,340,041 - -1.5 5,340,043 - -0.3 5,340,045 + -1.9 5,340,046 - +0.3 5,340,219 + -1.7 5,340,219 + -1.9 5,340,220 - +0.9 5,340,220 - -3.6 5,340,220 - -2.9 5,340,220 - -1.9 5,340,238 - -1.2 5,340,241 - +0.2 5,340,324 + Pf1N1B4_4835 0.12 -4.2 5,340,712 - -2.5 5,341,920 + Pf1N1B4_4835 0.89 -1.3 5,341,993 - -1.8 5,341,993 - -0.8 5,341,993 - -2.4 5,342,001 + -1.0 5,342,066 + -0.8 5,342,066 + -1.7 5,342,066 + -1.8 5,342,066 + +1.5 5,342,103 - -0.0 5,342,191 - -1.3 5,342,242 - Pf1N1B4_4836 0.41 -1.2 5,342,242 - Pf1N1B4_4836 0.41 +1.5 5,342,242 - Pf1N1B4_4836 0.41 -0.8 5,342,252 + Pf1N1B4_4836 0.46 +0.4 5,342,252 + Pf1N1B4_4836 0.46 -0.2 5,342,253 - Pf1N1B4_4836 0.47 +0.1 5,342,253 - Pf1N1B4_4836 0.47 -2.3 5,342,369 + -1.2 5,342,461 - Pf1N1B4_4837 0.44 +0.5 5,342,461 - Pf1N1B4_4837 0.44 -1.5 5,342,461 - Pf1N1B4_4837 0.44 +1.5 5,342,536 + Pf1N1B4_4837 0.88 -1.3 5,342,537 - Pf1N1B4_4837 0.89 +0.5 5,342,537 - Pf1N1B4_4837 0.89 -1.4 5,342,537 - Pf1N1B4_4837 0.89 +2.3 5,342,611 - -0.0 5,342,716 - -0.3 5,342,716 - -1.4 5,342,748 - -1.0 5,342,789 - -0.8 5,343,022 - -1.4 5,343,022 - +0.1 5,343,023 + -0.4
Or see this region's nucleotide sequence