Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4751 and Pf1N1B4_4752 are separated by 222 nucleotides Pf1N1B4_4752 and Pf1N1B4_4753 are separated by 51 nucleotides Pf1N1B4_4753 and Pf1N1B4_4754 are separated by 106 nucleotides
Pf1N1B4_4751 - Ku domain protein, at 5,248,957 to 5,249,817
_4751
Pf1N1B4_4752 - FIG00962948: hypothetical protein, at 5,250,040 to 5,250,384
_4752
Pf1N1B4_4753 - Soluble aldose sugar dehydrogenase, PQQ-dependent (EC 1.1.5.-), at 5,250,436 to 5,251,587
_4753
Pf1N1B4_4754 - GNAT family acetyltransferase VC2332, at 5,251,694 to 5,252,791
_4754
Position (kb)
5250
5251
5252 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5249.450 kb on + strand at 5249.451 kb on - strand, within Pf1N1B4_4751 at 5249.451 kb on - strand at 5249.483 kb on + strand, within Pf1N1B4_4751 at 5249.484 kb on - strand, within Pf1N1B4_4751 at 5249.484 kb on - strand, within Pf1N1B4_4751 at 5249.487 kb on - strand, within Pf1N1B4_4751 at 5249.487 kb on - strand, within Pf1N1B4_4751 at 5249.603 kb on + strand, within Pf1N1B4_4751 at 5249.604 kb on - strand at 5249.619 kb on - strand, within Pf1N1B4_4751 at 5249.841 kb on - strand at 5249.841 kb on - strand at 5249.841 kb on - strand at 5249.927 kb on - strand at 5249.948 kb on - strand at 5249.951 kb on + strand at 5250.015 kb on - strand at 5250.376 kb on + strand at 5250.377 kb on - strand at 5250.413 kb on - strand at 5250.444 kb on + strand at 5250.526 kb on - strand at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.773 kb on - strand, within Pf1N1B4_4753 at 5250.773 kb on - strand, within Pf1N1B4_4753 at 5250.773 kb on - strand at 5250.773 kb on - strand, within Pf1N1B4_4753 at 5250.949 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.508 kb on + strand at 5251.508 kb on + strand at 5251.534 kb on + strand at 5251.534 kb on + strand at 5251.534 kb on + strand at 5251.535 kb on - strand at 5251.535 kb on - strand at 5251.567 kb on + strand at 5251.567 kb on + strand at 5251.842 kb on + strand, within Pf1N1B4_4754 at 5251.950 kb on - strand, within Pf1N1B4_4754 at 5252.028 kb on - strand, within Pf1N1B4_4754 at 5252.028 kb on - strand, within Pf1N1B4_4754 at 5252.040 kb on - strand, within Pf1N1B4_4754 at 5252.102 kb on + strand at 5252.103 kb on - strand, within Pf1N1B4_4754 at 5252.311 kb on - strand, within Pf1N1B4_4754 at 5252.392 kb on - strand, within Pf1N1B4_4754 at 5252.434 kb on - strand at 5252.578 kb on - strand, within Pf1N1B4_4754
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 5,249,450 + +2.0 5,249,451 - Pf1N1B4_4751 0.57 +1.0 5,249,451 - +1.4 5,249,483 + Pf1N1B4_4751 0.61 -0.0 5,249,484 - Pf1N1B4_4751 0.61 -2.1 5,249,484 - Pf1N1B4_4751 0.61 -0.4 5,249,487 - Pf1N1B4_4751 0.62 -0.3 5,249,487 - Pf1N1B4_4751 0.62 -1.3 5,249,603 + Pf1N1B4_4751 0.75 +0.6 5,249,604 - -0.7 5,249,619 - Pf1N1B4_4751 0.77 -0.2 5,249,841 - +0.4 5,249,841 - +0.4 5,249,841 - +1.1 5,249,927 - -1.4 5,249,948 - +1.6 5,249,951 + +3.3 5,250,015 - +0.8 5,250,376 + -0.7 5,250,377 - -0.9 5,250,413 - -0.3 5,250,444 + -1.4 5,250,526 - -0.0 5,250,772 + Pf1N1B4_4753 0.29 +0.4 5,250,772 + Pf1N1B4_4753 0.29 +0.4 5,250,772 + Pf1N1B4_4753 0.29 -0.8 5,250,772 + Pf1N1B4_4753 0.29 +1.3 5,250,772 + Pf1N1B4_4753 0.29 -0.7 5,250,772 + Pf1N1B4_4753 0.29 +1.4 5,250,773 - Pf1N1B4_4753 0.29 +0.4 5,250,773 - Pf1N1B4_4753 0.29 -1.2 5,250,773 - +0.4 5,250,773 - Pf1N1B4_4753 0.29 -0.7 5,250,949 + Pf1N1B4_4753 0.45 +0.6 5,251,255 + Pf1N1B4_4753 0.71 -0.7 5,251,255 + Pf1N1B4_4753 0.71 +1.6 5,251,255 + Pf1N1B4_4753 0.71 +0.1 5,251,255 + Pf1N1B4_4753 0.71 +0.6 5,251,255 + Pf1N1B4_4753 0.71 +0.7 5,251,255 + Pf1N1B4_4753 0.71 -0.4 5,251,508 + +0.9 5,251,508 + +0.5 5,251,534 + -1.0 5,251,534 + -1.6 5,251,534 + -1.6 5,251,535 - +0.4 5,251,535 - +0.8 5,251,567 + -0.2 5,251,567 + -1.2 5,251,842 + Pf1N1B4_4754 0.13 -1.9 5,251,950 - Pf1N1B4_4754 0.23 +0.3 5,252,028 - Pf1N1B4_4754 0.30 +0.4 5,252,028 - Pf1N1B4_4754 0.30 -0.4 5,252,040 - Pf1N1B4_4754 0.32 +0.8 5,252,102 + +1.3 5,252,103 - Pf1N1B4_4754 0.37 -0.7 5,252,311 - Pf1N1B4_4754 0.56 +0.8 5,252,392 - Pf1N1B4_4754 0.64 +0.4 5,252,434 - -0.4 5,252,578 - Pf1N1B4_4754 0.81 -0.4
Or see this region's nucleotide sequence