Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4239 and Pf1N1B4_4240 are separated by 137 nucleotides Pf1N1B4_4240 and Pf1N1B4_4241 are separated by 273 nucleotides Pf1N1B4_4241 and Pf1N1B4_4242 are separated by 225 nucleotides
Pf1N1B4_4239 - hypothetical protein, at 4,698,425 to 4,698,976
_4239
Pf1N1B4_4240 - hypothetical protein, at 4,699,114 to 4,699,263
_4240
Pf1N1B4_4241 - Dienelactone hydrolase family, at 4,699,537 to 4,700,424
_4241
Pf1N1B4_4242 - cell processes; transport of small molecules; amino acids, amines, peptides, at 4,700,650 to 4,702,215
_4242
Position (kb)
4699
4700
4701 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4698.726 kb on + strand, within Pf1N1B4_4239 at 4698.727 kb on - strand, within Pf1N1B4_4239 at 4698.732 kb on + strand at 4698.733 kb on - strand, within Pf1N1B4_4239 at 4698.733 kb on - strand, within Pf1N1B4_4239 at 4698.873 kb on + strand, within Pf1N1B4_4239 at 4698.873 kb on + strand, within Pf1N1B4_4239 at 4698.873 kb on + strand at 4698.874 kb on - strand, within Pf1N1B4_4239 at 4698.880 kb on - strand, within Pf1N1B4_4239 at 4699.076 kb on + strand at 4699.109 kb on - strand at 4699.115 kb on + strand at 4699.165 kb on - strand, within Pf1N1B4_4240 at 4699.168 kb on + strand, within Pf1N1B4_4240 at 4699.169 kb on - strand, within Pf1N1B4_4240 at 4699.281 kb on + strand at 4699.282 kb on - strand at 4699.520 kb on + strand at 4699.649 kb on + strand, within Pf1N1B4_4241 at 4699.653 kb on + strand, within Pf1N1B4_4241 at 4699.653 kb on + strand, within Pf1N1B4_4241 at 4699.653 kb on + strand, within Pf1N1B4_4241 at 4699.655 kb on + strand, within Pf1N1B4_4241 at 4699.655 kb on + strand at 4699.655 kb on + strand, within Pf1N1B4_4241 at 4699.655 kb on + strand, within Pf1N1B4_4241 at 4699.656 kb on - strand, within Pf1N1B4_4241 at 4699.664 kb on + strand, within Pf1N1B4_4241 at 4699.664 kb on + strand, within Pf1N1B4_4241 at 4699.664 kb on + strand, within Pf1N1B4_4241 at 4699.665 kb on - strand, within Pf1N1B4_4241 at 4699.665 kb on - strand, within Pf1N1B4_4241 at 4699.665 kb on - strand, within Pf1N1B4_4241 at 4699.799 kb on + strand, within Pf1N1B4_4241 at 4699.799 kb on + strand, within Pf1N1B4_4241 at 4699.819 kb on + strand, within Pf1N1B4_4241 at 4700.087 kb on + strand, within Pf1N1B4_4241 at 4700.088 kb on - strand, within Pf1N1B4_4241 at 4700.201 kb on + strand, within Pf1N1B4_4241 at 4700.210 kb on + strand, within Pf1N1B4_4241 at 4700.210 kb on + strand, within Pf1N1B4_4241 at 4700.210 kb on + strand, within Pf1N1B4_4241 at 4700.211 kb on - strand, within Pf1N1B4_4241 at 4700.261 kb on + strand, within Pf1N1B4_4241 at 4700.262 kb on - strand, within Pf1N1B4_4241 at 4700.262 kb on - strand, within Pf1N1B4_4241 at 4700.367 kb on - strand at 4700.369 kb on + strand at 4700.369 kb on + strand at 4700.370 kb on - strand at 4700.375 kb on + strand at 4700.487 kb on + strand at 4700.487 kb on + strand at 4700.488 kb on - strand at 4700.546 kb on + strand at 4700.601 kb on + strand at 4700.889 kb on - strand at 4700.889 kb on - strand, within Pf1N1B4_4242 at 4700.889 kb on - strand, within Pf1N1B4_4242 at 4700.943 kb on - strand, within Pf1N1B4_4242 at 4700.946 kb on + strand, within Pf1N1B4_4242 at 4700.947 kb on - strand, within Pf1N1B4_4242 at 4700.947 kb on - strand, within Pf1N1B4_4242 at 4701.021 kb on - strand, within Pf1N1B4_4242
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 4,698,726 + Pf1N1B4_4239 0.55 +0.2 4,698,727 - Pf1N1B4_4239 0.55 -2.1 4,698,732 + +0.6 4,698,733 - Pf1N1B4_4239 0.56 +0.2 4,698,733 - Pf1N1B4_4239 0.56 -0.4 4,698,873 + Pf1N1B4_4239 0.81 -0.6 4,698,873 + Pf1N1B4_4239 0.81 +0.6 4,698,873 + -0.0 4,698,874 - Pf1N1B4_4239 0.81 -1.0 4,698,880 - Pf1N1B4_4239 0.82 -0.7 4,699,076 + -0.8 4,699,109 - -2.0 4,699,115 + -1.1 4,699,165 - Pf1N1B4_4240 0.34 -0.8 4,699,168 + Pf1N1B4_4240 0.36 -0.3 4,699,169 - Pf1N1B4_4240 0.37 +0.2 4,699,281 + -0.1 4,699,282 - -0.8 4,699,520 + +0.3 4,699,649 + Pf1N1B4_4241 0.13 +2.7 4,699,653 + Pf1N1B4_4241 0.13 +1.0 4,699,653 + Pf1N1B4_4241 0.13 -1.9 4,699,653 + Pf1N1B4_4241 0.13 -0.0 4,699,655 + Pf1N1B4_4241 0.13 +1.0 4,699,655 + -0.8 4,699,655 + Pf1N1B4_4241 0.13 +1.5 4,699,655 + Pf1N1B4_4241 0.13 +1.2 4,699,656 - Pf1N1B4_4241 0.13 -0.8 4,699,664 + Pf1N1B4_4241 0.14 +1.1 4,699,664 + Pf1N1B4_4241 0.14 -0.8 4,699,664 + Pf1N1B4_4241 0.14 +1.0 4,699,665 - Pf1N1B4_4241 0.14 +0.9 4,699,665 - Pf1N1B4_4241 0.14 +1.0 4,699,665 - Pf1N1B4_4241 0.14 -1.4 4,699,799 + Pf1N1B4_4241 0.30 +0.8 4,699,799 + Pf1N1B4_4241 0.30 -1.3 4,699,819 + Pf1N1B4_4241 0.32 -0.9 4,700,087 + Pf1N1B4_4241 0.62 +0.3 4,700,088 - Pf1N1B4_4241 0.62 +0.6 4,700,201 + Pf1N1B4_4241 0.75 +1.0 4,700,210 + Pf1N1B4_4241 0.76 -0.3 4,700,210 + Pf1N1B4_4241 0.76 +1.1 4,700,210 + Pf1N1B4_4241 0.76 +0.2 4,700,211 - Pf1N1B4_4241 0.76 +0.4 4,700,261 + Pf1N1B4_4241 0.82 -0.7 4,700,262 - Pf1N1B4_4241 0.82 -0.6 4,700,262 - Pf1N1B4_4241 0.82 +2.5 4,700,367 - -0.1 4,700,369 + -0.0 4,700,369 + -1.0 4,700,370 - +0.9 4,700,375 + +0.5 4,700,487 + -0.5 4,700,487 + -0.0 4,700,488 - +2.2 4,700,546 + -0.5 4,700,601 + -1.2 4,700,889 - -1.8 4,700,889 - Pf1N1B4_4242 0.15 +0.8 4,700,889 - Pf1N1B4_4242 0.15 -1.1 4,700,943 - Pf1N1B4_4242 0.19 -0.6 4,700,946 + Pf1N1B4_4242 0.19 +1.6 4,700,947 - Pf1N1B4_4242 0.19 -1.3 4,700,947 - Pf1N1B4_4242 0.19 +2.1 4,701,021 - Pf1N1B4_4242 0.24 -0.6
Or see this region's nucleotide sequence