Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_406 and Pf1N1B4_407 are separated by 220 nucleotides Pf1N1B4_407 and Pf1N1B4_408 overlap by 4 nucleotides Pf1N1B4_408 and Pf1N1B4_409 are separated by 245 nucleotides
Pf1N1B4_406 - Purine-cytosine permease, at 474,219 to 475,685
_406
Pf1N1B4_407 - ADP-ribosylglycohydrolase YegU (EC 3.2.-.-), at 475,906 to 476,904
_407
Pf1N1B4_408 - Transcriptional regulator, GntR family, at 476,901 to 477,578
_408
Pf1N1B4_409 - ABC transporter for D-galactose/L-arabinose, permease component <small>(from data)</small>, at 477,824 to 478,792
_409
Position (kb) 475
476
477 Strain fitness (log2 ratio) -2
-1
0
1
2 at 475.135 kb on + strand, within Pf1N1B4_406 at 475.135 kb on + strand, within Pf1N1B4_406 at 475.135 kb on + strand, within Pf1N1B4_406 at 475.135 kb on + strand, within Pf1N1B4_406 at 475.135 kb on + strand, within Pf1N1B4_406 at 475.135 kb on + strand at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.563 kb on - strand at 475.685 kb on + strand at 475.685 kb on + strand at 475.685 kb on + strand at 475.685 kb on + strand at 475.686 kb on - strand at 475.686 kb on - strand at 475.686 kb on - strand at 475.686 kb on - strand at 475.740 kb on - strand at 475.794 kb on + strand at 475.795 kb on - strand at 475.795 kb on - strand at 475.798 kb on - strand at 475.837 kb on + strand at 475.861 kb on - strand at 475.864 kb on - strand at 475.864 kb on - strand at 475.902 kb on - strand at 475.966 kb on - strand at 476.184 kb on - strand, within Pf1N1B4_407 at 476.256 kb on + strand at 476.490 kb on + strand, within Pf1N1B4_407 at 476.491 kb on - strand, within Pf1N1B4_407 at 476.491 kb on - strand, within Pf1N1B4_407 at 476.870 kb on + strand at 476.870 kb on + strand at 476.870 kb on + strand at 476.870 kb on + strand at 476.871 kb on - strand at 476.871 kb on - strand at 476.871 kb on - strand at 476.871 kb on - strand at 476.871 kb on - strand at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.993 kb on - strand, within Pf1N1B4_408 at 476.993 kb on - strand, within Pf1N1B4_408 at 476.993 kb on - strand, within Pf1N1B4_408 at 476.993 kb on - strand, within Pf1N1B4_408 at 477.106 kb on + strand, within Pf1N1B4_408 at 477.106 kb on + strand, within Pf1N1B4_408 at 477.106 kb on + strand, within Pf1N1B4_408 at 477.106 kb on + strand, within Pf1N1B4_408 at 477.324 kb on - strand, within Pf1N1B4_408 at 477.522 kb on + strand at 477.522 kb on + strand at 477.522 kb on + strand at 477.522 kb on + strand at 477.608 kb on + strand at 477.609 kb on - strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.637 kb on + strand at 477.637 kb on + strand at 477.637 kb on + strand at 477.637 kb on + strand at 477.667 kb on + strand at 477.668 kb on - strand at 477.886 kb on - strand at 477.886 kb on - strand at 477.886 kb on - strand at 477.886 kb on - strand at 477.891 kb on + strand at 477.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 475,135 + Pf1N1B4_406 0.62 -0.5 475,135 + Pf1N1B4_406 0.62 -0.5 475,135 + Pf1N1B4_406 0.62 +0.5 475,135 + Pf1N1B4_406 0.62 +1.3 475,135 + Pf1N1B4_406 0.62 +0.3 475,135 + +0.8 475,136 - Pf1N1B4_406 0.63 -1.0 475,136 - Pf1N1B4_406 0.63 -1.0 475,136 - Pf1N1B4_406 0.63 +1.5 475,136 - Pf1N1B4_406 0.63 -0.5 475,136 - Pf1N1B4_406 0.63 +0.1 475,136 - Pf1N1B4_406 0.63 -2.2 475,136 - Pf1N1B4_406 0.63 +1.1 475,563 - -0.0 475,685 + -1.0 475,685 + +1.5 475,685 + -0.0 475,685 + +2.5 475,686 - +0.1 475,686 - +0.9 475,686 - +2.1 475,686 - -0.5 475,740 - +0.1 475,794 + -1.9 475,795 - -1.0 475,795 - -0.8 475,798 - -1.3 475,837 + +1.0 475,861 - -1.5 475,864 - -1.5 475,864 - -0.0 475,902 - +0.2 475,966 - +1.9 476,184 - Pf1N1B4_407 0.28 +1.8 476,256 + +0.8 476,490 + Pf1N1B4_407 0.58 +1.1 476,491 - Pf1N1B4_407 0.59 +1.3 476,491 - Pf1N1B4_407 0.59 +0.2 476,870 + -0.2 476,870 + +0.4 476,870 + +1.0 476,870 + +0.4 476,871 - +1.9 476,871 - +0.8 476,871 - -1.2 476,871 - +0.9 476,871 - +0.9 476,992 + Pf1N1B4_408 0.13 -0.5 476,992 + Pf1N1B4_408 0.13 -0.5 476,992 + Pf1N1B4_408 0.13 -0.4 476,992 + Pf1N1B4_408 0.13 -0.5 476,992 + Pf1N1B4_408 0.13 -1.3 476,992 + Pf1N1B4_408 0.13 -0.8 476,992 + Pf1N1B4_408 0.13 -2.0 476,992 + Pf1N1B4_408 0.13 +1.1 476,992 + Pf1N1B4_408 0.13 -1.3 476,993 - Pf1N1B4_408 0.14 +1.1 476,993 - Pf1N1B4_408 0.14 +0.2 476,993 - Pf1N1B4_408 0.14 -1.8 476,993 - Pf1N1B4_408 0.14 -1.2 477,106 + Pf1N1B4_408 0.30 -2.5 477,106 + Pf1N1B4_408 0.30 +1.5 477,106 + Pf1N1B4_408 0.30 -1.1 477,106 + Pf1N1B4_408 0.30 -1.5 477,324 - Pf1N1B4_408 0.62 -0.8 477,522 + +1.9 477,522 + +1.2 477,522 + -0.0 477,522 + -0.6 477,608 + -2.2 477,609 - +0.5 477,616 + -0.0 477,616 + -1.3 477,616 + -1.5 477,616 + -0.0 477,616 + +1.0 477,616 + -0.0 477,616 + +0.4 477,616 + -1.9 477,616 + +0.6 477,616 + -0.9 477,617 - -1.0 477,617 - +0.5 477,617 - -0.5 477,617 - +0.9 477,617 - -0.5 477,617 - -1.0 477,617 - +0.5 477,617 - -0.5 477,637 + +1.7 477,637 + -2.7 477,637 + +1.2 477,637 + -0.1 477,667 + +0.2 477,668 - -0.5 477,886 - +1.1 477,886 - -1.2 477,886 - -1.5 477,886 - -0.8 477,891 + -0.8 477,892 - -0.6
Or see this region's nucleotide sequence