Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4051 and Pf1N1B4_4052 are separated by 493 nucleotides Pf1N1B4_4052 and Pf1N1B4_4053 are separated by 61 nucleotides Pf1N1B4_4053 and Pf1N1B4_4054 are separated by 341 nucleotides Pf1N1B4_4054 and Pf1N1B4_4055 are separated by 30 nucleotides
Pf1N1B4_4051 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,461 to 4,504,533
_4051
Pf1N1B4_4052 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,505,027 to 4,506,283
_4052
Pf1N1B4_4053 - Cold shock protein CspD, at 4,506,345 to 4,506,626
_4053
Pf1N1B4_4054 - ATP-dependent Clp protease adaptor protein ClpS, at 4,506,968 to 4,507,213
_4054
Pf1N1B4_4055 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 4,507,244 to 4,509,514
_4055
Position (kb)
4505
4506
4507 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4504.073 kb on + strand, within Pf1N1B4_4051 at 4504.073 kb on + strand, within Pf1N1B4_4051 at 4504.472 kb on + strand at 4504.472 kb on + strand at 4504.472 kb on + strand at 4504.506 kb on + strand at 4504.506 kb on + strand at 4504.508 kb on + strand at 4504.508 kb on + strand at 4504.560 kb on + strand at 4504.692 kb on - strand at 4504.727 kb on + strand at 4504.769 kb on + strand at 4504.770 kb on - strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.780 kb on - strand at 4504.805 kb on - strand at 4504.805 kb on - strand at 4504.805 kb on - strand at 4504.830 kb on + strand at 4504.830 kb on + strand at 4504.830 kb on + strand at 4504.856 kb on + strand at 4504.856 kb on + strand at 4504.910 kb on + strand at 4504.910 kb on + strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.953 kb on - strand at 4504.971 kb on + strand at 4504.972 kb on - strand at 4504.972 kb on - strand at 4504.981 kb on - strand at 4505.022 kb on - strand at 4505.022 kb on - strand at 4505.022 kb on - strand at 4505.140 kb on + strand at 4505.141 kb on - strand at 4505.153 kb on - strand, within Pf1N1B4_4052 at 4505.237 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.316 kb on - strand, within Pf1N1B4_4052 at 4505.316 kb on - strand, within Pf1N1B4_4052 at 4505.319 kb on + strand, within Pf1N1B4_4052 at 4505.319 kb on + strand, within Pf1N1B4_4052 at 4505.319 kb on + strand at 4505.320 kb on - strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand at 4505.406 kb on - strand, within Pf1N1B4_4052 at 4505.406 kb on - strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.511 kb on - strand at 4505.677 kb on + strand, within Pf1N1B4_4052 at 4505.678 kb on + strand, within Pf1N1B4_4052 at 4505.678 kb on + strand, within Pf1N1B4_4052 at 4505.678 kb on + strand, within Pf1N1B4_4052 at 4505.679 kb on - strand, within Pf1N1B4_4052 at 4505.679 kb on - strand, within Pf1N1B4_4052 at 4505.918 kb on + strand, within Pf1N1B4_4052 at 4505.954 kb on + strand, within Pf1N1B4_4052 at 4505.955 kb on - strand, within Pf1N1B4_4052 at 4505.955 kb on - strand, within Pf1N1B4_4052 at 4506.065 kb on + strand, within Pf1N1B4_4052 at 4506.203 kb on + strand at 4506.203 kb on + strand at 4506.203 kb on + strand at 4506.204 kb on - strand at 4506.204 kb on - strand at 4506.281 kb on + strand at 4506.281 kb on + strand at 4506.281 kb on + strand at 4506.282 kb on - strand at 4506.487 kb on + strand, within Pf1N1B4_4053 at 4506.488 kb on - strand, within Pf1N1B4_4053 at 4506.512 kb on - strand, within Pf1N1B4_4053 at 4506.555 kb on + strand, within Pf1N1B4_4053 at 4506.555 kb on + strand, within Pf1N1B4_4053 at 4506.556 kb on - strand, within Pf1N1B4_4053 at 4506.556 kb on - strand, within Pf1N1B4_4053 at 4506.556 kb on - strand, within Pf1N1B4_4053 at 4506.663 kb on + strand at 4506.664 kb on - strand at 4506.686 kb on + strand at 4506.687 kb on - strand at 4506.687 kb on - strand at 4506.698 kb on + strand at 4506.739 kb on - strand at 4506.821 kb on + strand at 4506.821 kb on + strand at 4506.822 kb on - strand at 4506.822 kb on - strand at 4506.876 kb on + strand at 4506.903 kb on + strand at 4506.903 kb on + strand at 4506.943 kb on + strand at 4506.954 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.972 kb on - strand at 4506.972 kb on - strand at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.999 kb on - strand, within Pf1N1B4_4054 at 4506.999 kb on - strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.105 kb on - strand, within Pf1N1B4_4054 at 4507.160 kb on + strand, within Pf1N1B4_4054 at 4507.244 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 4,504,073 + Pf1N1B4_4051 0.78 +0.9 4,504,073 + Pf1N1B4_4051 0.78 -0.5 4,504,472 + -2.3 4,504,472 + -0.8 4,504,472 + -1.4 4,504,506 + +1.9 4,504,506 + -0.7 4,504,508 + +1.2 4,504,508 + -0.3 4,504,560 + +0.1 4,504,692 - -0.8 4,504,727 + -1.0 4,504,769 + +3.0 4,504,770 - -0.4 4,504,771 + -0.1 4,504,771 + +0.2 4,504,771 + +1.2 4,504,771 + +0.3 4,504,771 + -0.9 4,504,771 + -0.8 4,504,771 + +0.0 4,504,771 + +0.6 4,504,771 + -0.8 4,504,771 + +1.6 4,504,771 + +1.4 4,504,771 + +0.6 4,504,771 + -1.5 4,504,771 + +0.3 4,504,771 + -0.1 4,504,771 + -1.0 4,504,771 + +0.8 4,504,772 - +0.6 4,504,772 - +0.7 4,504,772 - -0.4 4,504,772 - +1.6 4,504,772 - -1.0 4,504,772 - -0.2 4,504,775 + -0.8 4,504,775 + +0.5 4,504,775 + -1.0 4,504,775 + -0.5 4,504,775 + -0.6 4,504,775 + -0.4 4,504,775 + -1.0 4,504,775 + -0.8 4,504,776 - +2.3 4,504,776 - -1.4 4,504,776 - -0.4 4,504,776 - -1.8 4,504,776 - +1.6 4,504,776 - +0.6 4,504,776 - +0.6 4,504,780 - +1.4 4,504,805 - +0.6 4,504,805 - -2.9 4,504,805 - +0.6 4,504,830 + +1.5 4,504,830 + -1.5 4,504,830 + +1.3 4,504,856 + +1.3 4,504,856 + -1.0 4,504,910 + +1.2 4,504,910 + +1.0 4,504,911 - +4.0 4,504,911 - -0.5 4,504,911 - -1.0 4,504,911 - -1.9 4,504,911 - +0.1 4,504,911 - -2.0 4,504,952 + -1.2 4,504,952 + +0.6 4,504,952 + +0.6 4,504,952 + -0.2 4,504,952 + -0.4 4,504,953 - +0.3 4,504,971 + +0.5 4,504,972 - -0.4 4,504,972 - -0.8 4,504,981 - +0.2 4,505,022 - -2.4 4,505,022 - +0.6 4,505,022 - -0.5 4,505,140 + -0.9 4,505,141 - -1.0 4,505,153 - Pf1N1B4_4052 0.10 -0.7 4,505,237 + Pf1N1B4_4052 0.17 -1.2 4,505,315 + Pf1N1B4_4052 0.23 -2.0 4,505,315 + Pf1N1B4_4052 0.23 -0.9 4,505,315 + Pf1N1B4_4052 0.23 -1.9 4,505,315 + Pf1N1B4_4052 0.23 -0.4 4,505,316 - Pf1N1B4_4052 0.23 -2.2 4,505,316 - Pf1N1B4_4052 0.23 -2.0 4,505,319 + Pf1N1B4_4052 0.23 +0.9 4,505,319 + Pf1N1B4_4052 0.23 -2.4 4,505,319 + +0.6 4,505,320 - Pf1N1B4_4052 0.23 -1.2 4,505,405 + Pf1N1B4_4052 0.30 -0.1 4,505,405 + Pf1N1B4_4052 0.30 -0.5 4,505,405 + Pf1N1B4_4052 0.30 -0.1 4,505,405 + Pf1N1B4_4052 0.30 -0.5 4,505,405 + Pf1N1B4_4052 0.30 -0.9 4,505,405 + -2.0 4,505,406 - Pf1N1B4_4052 0.30 -1.6 4,505,406 - Pf1N1B4_4052 0.30 +1.0 4,505,510 + Pf1N1B4_4052 0.38 -2.1 4,505,510 + Pf1N1B4_4052 0.38 +0.0 4,505,510 + Pf1N1B4_4052 0.38 -0.3 4,505,511 - -1.0 4,505,677 + Pf1N1B4_4052 0.52 -1.6 4,505,678 + Pf1N1B4_4052 0.52 -0.8 4,505,678 + Pf1N1B4_4052 0.52 -2.0 4,505,678 + Pf1N1B4_4052 0.52 -0.4 4,505,679 - Pf1N1B4_4052 0.52 -1.8 4,505,679 - Pf1N1B4_4052 0.52 -1.5 4,505,918 + Pf1N1B4_4052 0.71 -1.7 4,505,954 + Pf1N1B4_4052 0.74 -1.5 4,505,955 - Pf1N1B4_4052 0.74 -0.4 4,505,955 - Pf1N1B4_4052 0.74 +0.0 4,506,065 + Pf1N1B4_4052 0.83 -1.8 4,506,203 + +0.8 4,506,203 + -1.0 4,506,203 + -1.2 4,506,204 - -1.1 4,506,204 - -1.7 4,506,281 + +0.6 4,506,281 + -1.3 4,506,281 + +0.6 4,506,282 - -1.5 4,506,487 + Pf1N1B4_4053 0.50 -0.1 4,506,488 - Pf1N1B4_4053 0.51 -1.0 4,506,512 - Pf1N1B4_4053 0.59 -2.7 4,506,555 + Pf1N1B4_4053 0.74 -1.7 4,506,555 + Pf1N1B4_4053 0.74 +0.3 4,506,556 - Pf1N1B4_4053 0.75 +0.6 4,506,556 - Pf1N1B4_4053 0.75 -4.1 4,506,556 - Pf1N1B4_4053 0.75 -2.5 4,506,663 + +1.3 4,506,664 - -0.7 4,506,686 + -1.5 4,506,687 - -0.7 4,506,687 - -1.8 4,506,698 + +0.4 4,506,739 - +1.9 4,506,821 + +0.6 4,506,821 + +0.8 4,506,822 - +2.5 4,506,822 - -2.2 4,506,876 + -1.5 4,506,903 + +0.5 4,506,903 + -0.6 4,506,943 + -0.0 4,506,954 + -1.0 4,506,971 + -3.3 4,506,971 + -1.3 4,506,971 + -1.1 4,506,971 + -1.1 4,506,971 + -2.0 4,506,971 + -1.4 4,506,971 + -0.1 4,506,971 + -2.1 4,506,971 + -1.4 4,506,971 + -0.9 4,506,971 + -1.0 4,506,971 + -0.8 4,506,972 - -2.0 4,506,972 - -2.1 4,506,998 + Pf1N1B4_4054 0.12 -0.6 4,506,998 + Pf1N1B4_4054 0.12 -0.2 4,506,998 + Pf1N1B4_4054 0.12 -0.1 4,506,998 + Pf1N1B4_4054 0.12 -1.8 4,506,998 + Pf1N1B4_4054 0.12 +0.0 4,506,999 - Pf1N1B4_4054 0.13 -1.0 4,506,999 - Pf1N1B4_4054 0.13 -0.7 4,507,104 + Pf1N1B4_4054 0.55 -1.2 4,507,104 + Pf1N1B4_4054 0.55 -0.4 4,507,104 + Pf1N1B4_4054 0.55 +0.6 4,507,104 + Pf1N1B4_4054 0.55 -2.1 4,507,104 + Pf1N1B4_4054 0.55 -1.2 4,507,104 + Pf1N1B4_4054 0.55 +0.1 4,507,104 + Pf1N1B4_4054 0.55 -1.0 4,507,104 + Pf1N1B4_4054 0.55 +0.7 4,507,104 + Pf1N1B4_4054 0.55 +1.0 4,507,104 + Pf1N1B4_4054 0.55 -1.6 4,507,104 + Pf1N1B4_4054 0.55 -1.0 4,507,105 - Pf1N1B4_4054 0.56 -0.2 4,507,160 + Pf1N1B4_4054 0.78 -2.1 4,507,244 - -1.7
Or see this region's nucleotide sequence