Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3992 and Pf1N1B4_3993 are separated by 165 nucleotides Pf1N1B4_3993 and Pf1N1B4_3994 are separated by 142 nucleotides
Pf1N1B4_3992 - Transcriptional regulator, LysR family, at 4,436,095 to 4,437,024
_3992
Pf1N1B4_3993 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 4,437,190 to 4,438,419
_3993
Pf1N1B4_3994 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 4,438,562 to 4,439,908
_3994
Position (kb)
4437
4438
4439 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4436.204 kb on + strand at 4436.256 kb on - strand, within Pf1N1B4_3992 at 4436.256 kb on - strand, within Pf1N1B4_3992 at 4436.336 kb on + strand, within Pf1N1B4_3992 at 4436.337 kb on - strand, within Pf1N1B4_3992 at 4436.337 kb on - strand, within Pf1N1B4_3992 at 4436.399 kb on + strand, within Pf1N1B4_3992 at 4436.428 kb on - strand, within Pf1N1B4_3992 at 4436.451 kb on - strand at 4436.513 kb on + strand, within Pf1N1B4_3992 at 4436.972 kb on + strand at 4437.051 kb on + strand at 4437.052 kb on - strand at 4437.078 kb on + strand at 4437.079 kb on - strand at 4437.079 kb on - strand at 4437.096 kb on + strand at 4437.099 kb on + strand at 4437.126 kb on + strand at 4437.140 kb on + strand at 4437.141 kb on - strand at 4437.174 kb on + strand at 4437.175 kb on - strand at 4437.181 kb on + strand at 4437.181 kb on + strand at 4437.181 kb on + strand at 4437.182 kb on - strand at 4437.256 kb on + strand at 4437.256 kb on + strand at 4437.256 kb on + strand at 4437.256 kb on + strand at 4437.256 kb on + strand at 4437.256 kb on + strand at 4437.256 kb on + strand at 4437.257 kb on - strand at 4437.257 kb on - strand at 4437.257 kb on - strand at 4438.147 kb on + strand, within Pf1N1B4_3993 at 4438.148 kb on - strand, within Pf1N1B4_3993 at 4438.294 kb on + strand at 4438.294 kb on + strand at 4438.294 kb on + strand, within Pf1N1B4_3993 at 4438.294 kb on + strand, within Pf1N1B4_3993 at 4438.294 kb on + strand, within Pf1N1B4_3993 at 4438.295 kb on - strand, within Pf1N1B4_3993 at 4438.295 kb on - strand, within Pf1N1B4_3993 at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.385 kb on - strand at 4438.385 kb on - strand at 4438.546 kb on + strand at 4438.547 kb on - strand at 4438.547 kb on - strand at 4438.555 kb on - strand at 4438.555 kb on - strand at 4438.555 kb on - strand at 4438.555 kb on - strand at 4438.566 kb on + strand at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.805 kb on - strand, within Pf1N1B4_3994 at 4438.805 kb on - strand, within Pf1N1B4_3994 at 4438.805 kb on - strand, within Pf1N1B4_3994 at 4438.805 kb on - strand, within Pf1N1B4_3994 at 4438.806 kb on + strand, within Pf1N1B4_3994 at 4438.806 kb on + strand at 4438.806 kb on + strand, within Pf1N1B4_3994 at 4438.807 kb on - strand, within Pf1N1B4_3994 at 4438.807 kb on - strand at 4438.807 kb on - strand, within Pf1N1B4_3994 at 4438.807 kb on - strand, within Pf1N1B4_3994 at 4439.070 kb on + strand, within Pf1N1B4_3994 at 4439.071 kb on - strand, within Pf1N1B4_3994 at 4439.184 kb on + strand, within Pf1N1B4_3994 at 4439.184 kb on + strand at 4439.184 kb on + strand, within Pf1N1B4_3994 at 4439.185 kb on - strand, within Pf1N1B4_3994 at 4439.185 kb on - strand, within Pf1N1B4_3994 at 4439.185 kb on - strand, within Pf1N1B4_3994 at 4439.322 kb on + strand, within Pf1N1B4_3994 at 4439.322 kb on + strand at 4439.349 kb on + strand, within Pf1N1B4_3994 at 4439.349 kb on + strand, within Pf1N1B4_3994 at 4439.349 kb on + strand, within Pf1N1B4_3994 at 4439.349 kb on + strand, within Pf1N1B4_3994 at 4439.350 kb on - strand, within Pf1N1B4_3994
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 4,436,204 + +1.6 4,436,256 - Pf1N1B4_3992 0.17 -0.9 4,436,256 - Pf1N1B4_3992 0.17 +0.8 4,436,336 + Pf1N1B4_3992 0.26 -0.6 4,436,337 - Pf1N1B4_3992 0.26 -0.1 4,436,337 - Pf1N1B4_3992 0.26 +1.9 4,436,399 + Pf1N1B4_3992 0.33 -0.8 4,436,428 - Pf1N1B4_3992 0.36 -0.9 4,436,451 - +0.3 4,436,513 + Pf1N1B4_3992 0.45 +0.0 4,436,972 + -2.1 4,437,051 + +1.6 4,437,052 - +0.4 4,437,078 + +0.1 4,437,079 - +0.1 4,437,079 - -1.1 4,437,096 + -0.2 4,437,099 + +0.8 4,437,126 + -0.4 4,437,140 + -0.5 4,437,141 - -3.0 4,437,174 + -0.5 4,437,175 - -2.7 4,437,181 + +1.2 4,437,181 + -0.9 4,437,181 + -2.1 4,437,182 - -1.7 4,437,256 + -1.0 4,437,256 + +0.1 4,437,256 + -2.1 4,437,256 + -1.8 4,437,256 + +0.1 4,437,256 + -0.7 4,437,256 + -1.7 4,437,257 - -1.9 4,437,257 - -2.1 4,437,257 - +0.4 4,438,147 + Pf1N1B4_3993 0.78 +0.2 4,438,148 - Pf1N1B4_3993 0.78 -2.2 4,438,294 + -0.4 4,438,294 + +0.1 4,438,294 + Pf1N1B4_3993 0.90 -1.4 4,438,294 + Pf1N1B4_3993 0.90 -2.4 4,438,294 + Pf1N1B4_3993 0.90 -3.2 4,438,295 - Pf1N1B4_3993 0.90 +1.2 4,438,295 - Pf1N1B4_3993 0.90 -0.1 4,438,300 + -1.2 4,438,300 + -2.6 4,438,300 + -0.5 4,438,300 + -2.6 4,438,300 + -0.8 4,438,300 + -0.2 4,438,300 + -1.5 4,438,300 + -2.4 4,438,300 + -0.7 4,438,300 + -2.4 4,438,300 + -1.9 4,438,300 + -0.2 4,438,300 + -0.7 4,438,300 + +1.6 4,438,300 + -2.3 4,438,300 + -1.6 4,438,301 - -0.2 4,438,301 - -0.8 4,438,301 - -0.9 4,438,301 - -1.9 4,438,301 - -0.2 4,438,301 - -3.6 4,438,301 - -1.7 4,438,301 - +0.1 4,438,384 + +0.6 4,438,384 + -1.8 4,438,384 + +0.1 4,438,384 + -1.6 4,438,384 + +0.2 4,438,384 + -0.4 4,438,384 + +0.1 4,438,384 + -0.9 4,438,384 + -1.2 4,438,384 + -0.2 4,438,384 + -0.2 4,438,385 - +0.6 4,438,385 - +2.6 4,438,546 + -0.8 4,438,547 - -0.0 4,438,547 - +0.1 4,438,555 - -1.7 4,438,555 - +0.5 4,438,555 - +0.1 4,438,555 - -1.0 4,438,566 + +0.8 4,438,802 + Pf1N1B4_3994 0.18 -1.5 4,438,802 + Pf1N1B4_3994 0.18 +0.2 4,438,802 + Pf1N1B4_3994 0.18 +0.4 4,438,802 + Pf1N1B4_3994 0.18 -1.5 4,438,802 + Pf1N1B4_3994 0.18 +0.6 4,438,802 + Pf1N1B4_3994 0.18 +1.6 4,438,802 + Pf1N1B4_3994 0.18 +0.1 4,438,802 + Pf1N1B4_3994 0.18 -2.4 4,438,802 + Pf1N1B4_3994 0.18 +0.5 4,438,802 + Pf1N1B4_3994 0.18 -0.2 4,438,802 + Pf1N1B4_3994 0.18 +0.5 4,438,802 + Pf1N1B4_3994 0.18 -1.7 4,438,803 - Pf1N1B4_3994 0.18 -2.1 4,438,803 - Pf1N1B4_3994 0.18 +0.2 4,438,803 - Pf1N1B4_3994 0.18 -1.2 4,438,803 - Pf1N1B4_3994 0.18 -1.7 4,438,803 - Pf1N1B4_3994 0.18 -1.5 4,438,803 - Pf1N1B4_3994 0.18 -1.2 4,438,803 - Pf1N1B4_3994 0.18 +2.5 4,438,803 - Pf1N1B4_3994 0.18 +0.2 4,438,805 - Pf1N1B4_3994 0.18 -0.5 4,438,805 - Pf1N1B4_3994 0.18 -0.8 4,438,805 - Pf1N1B4_3994 0.18 +1.1 4,438,805 - Pf1N1B4_3994 0.18 +1.3 4,438,806 + Pf1N1B4_3994 0.18 -0.2 4,438,806 + -0.4 4,438,806 + Pf1N1B4_3994 0.18 -0.2 4,438,807 - Pf1N1B4_3994 0.18 -2.4 4,438,807 - +1.1 4,438,807 - Pf1N1B4_3994 0.18 -2.1 4,438,807 - Pf1N1B4_3994 0.18 +1.4 4,439,070 + Pf1N1B4_3994 0.38 -1.3 4,439,071 - Pf1N1B4_3994 0.38 -1.9 4,439,184 + Pf1N1B4_3994 0.46 -0.4 4,439,184 + -1.4 4,439,184 + Pf1N1B4_3994 0.46 +1.6 4,439,185 - Pf1N1B4_3994 0.46 +2.0 4,439,185 - Pf1N1B4_3994 0.46 -0.5 4,439,185 - Pf1N1B4_3994 0.46 +0.2 4,439,322 + Pf1N1B4_3994 0.56 +0.3 4,439,322 + -0.1 4,439,349 + Pf1N1B4_3994 0.58 +0.6 4,439,349 + Pf1N1B4_3994 0.58 +1.1 4,439,349 + Pf1N1B4_3994 0.58 -0.0 4,439,349 + Pf1N1B4_3994 0.58 -0.5 4,439,350 - Pf1N1B4_3994 0.59 -0.4
Or see this region's nucleotide sequence