Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3839 and Pf1N1B4_3840 are separated by 337 nucleotides Pf1N1B4_3840 and Pf1N1B4_3841 are separated by 15 nucleotides Pf1N1B4_3841 and Pf1N1B4_3842 are separated by 230 nucleotides
Pf1N1B4_3839 - Nitrate/nitrite transporter, at 4,256,027 to 4,257,238
_3839
Pf1N1B4_3840 - Response regulator NasT, at 4,257,576 to 4,258,151
_3840
Pf1N1B4_3841 - Nitrate ABC transporter, nitrate-binding protein, at 4,258,167 to 4,259,378
_3841
Pf1N1B4_3842 - Dihydroorotate dehydrogenase (EC 1.3.3.1), at 4,259,609 to 4,260,628
_3842
Position (kb)
4258
4259
4260 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4257.180 kb on + strand at 4257.180 kb on + strand at 4257.181 kb on - strand at 4257.261 kb on + strand at 4257.399 kb on - strand at 4257.421 kb on + strand at 4257.434 kb on + strand at 4257.435 kb on - strand at 4257.667 kb on + strand at 4257.668 kb on - strand, within Pf1N1B4_3840 at 4257.668 kb on - strand, within Pf1N1B4_3840 at 4257.856 kb on + strand, within Pf1N1B4_3840 at 4257.856 kb on + strand, within Pf1N1B4_3840 at 4257.856 kb on + strand, within Pf1N1B4_3840 at 4257.856 kb on + strand, within Pf1N1B4_3840 at 4257.856 kb on + strand, within Pf1N1B4_3840 at 4257.856 kb on + strand, within Pf1N1B4_3840 at 4257.857 kb on - strand, within Pf1N1B4_3840 at 4257.857 kb on - strand, within Pf1N1B4_3840 at 4257.857 kb on - strand, within Pf1N1B4_3840 at 4257.857 kb on - strand, within Pf1N1B4_3840 at 4257.857 kb on - strand, within Pf1N1B4_3840 at 4257.857 kb on - strand at 4257.857 kb on - strand, within Pf1N1B4_3840 at 4258.142 kb on + strand at 4258.151 kb on + strand at 4258.152 kb on - strand at 4258.152 kb on - strand at 4258.341 kb on + strand, within Pf1N1B4_3841 at 4258.341 kb on + strand at 4258.341 kb on + strand, within Pf1N1B4_3841 at 4258.341 kb on + strand, within Pf1N1B4_3841 at 4258.341 kb on + strand, within Pf1N1B4_3841 at 4258.373 kb on - strand, within Pf1N1B4_3841 at 4258.442 kb on - strand, within Pf1N1B4_3841 at 4258.442 kb on - strand, within Pf1N1B4_3841 at 4258.757 kb on + strand, within Pf1N1B4_3841 at 4258.918 kb on + strand, within Pf1N1B4_3841 at 4258.918 kb on + strand, within Pf1N1B4_3841 at 4258.918 kb on + strand, within Pf1N1B4_3841 at 4258.918 kb on + strand, within Pf1N1B4_3841 at 4258.918 kb on + strand, within Pf1N1B4_3841 at 4258.919 kb on - strand, within Pf1N1B4_3841 at 4258.919 kb on - strand, within Pf1N1B4_3841 at 4259.237 kb on - strand, within Pf1N1B4_3841 at 4259.268 kb on + strand at 4259.268 kb on + strand at 4259.511 kb on + strand at 4259.511 kb on + strand at 4259.512 kb on - strand at 4259.512 kb on - strand at 4259.612 kb on + strand at 4259.612 kb on + strand at 4259.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 4,257,180 + -1.0 4,257,180 + -3.1 4,257,181 - -1.5 4,257,261 + -0.4 4,257,399 - -0.8 4,257,421 + +0.6 4,257,434 + -1.8 4,257,435 - -0.8 4,257,667 + -0.7 4,257,668 - Pf1N1B4_3840 0.16 -1.1 4,257,668 - Pf1N1B4_3840 0.16 +0.8 4,257,856 + Pf1N1B4_3840 0.49 -1.1 4,257,856 + Pf1N1B4_3840 0.49 +0.0 4,257,856 + Pf1N1B4_3840 0.49 -1.0 4,257,856 + Pf1N1B4_3840 0.49 -0.2 4,257,856 + Pf1N1B4_3840 0.49 -0.6 4,257,856 + Pf1N1B4_3840 0.49 +1.8 4,257,857 - Pf1N1B4_3840 0.49 +0.4 4,257,857 - Pf1N1B4_3840 0.49 -1.0 4,257,857 - Pf1N1B4_3840 0.49 +0.2 4,257,857 - Pf1N1B4_3840 0.49 -0.8 4,257,857 - Pf1N1B4_3840 0.49 -1.6 4,257,857 - +0.2 4,257,857 - Pf1N1B4_3840 0.49 -1.1 4,258,142 + -1.3 4,258,151 + +1.6 4,258,152 - +0.0 4,258,152 - -1.6 4,258,341 + Pf1N1B4_3841 0.14 -1.0 4,258,341 + -0.4 4,258,341 + Pf1N1B4_3841 0.14 +1.4 4,258,341 + Pf1N1B4_3841 0.14 +0.3 4,258,341 + Pf1N1B4_3841 0.14 -1.2 4,258,373 - Pf1N1B4_3841 0.17 +0.6 4,258,442 - Pf1N1B4_3841 0.23 +0.5 4,258,442 - Pf1N1B4_3841 0.23 +0.3 4,258,757 + Pf1N1B4_3841 0.49 +1.4 4,258,918 + Pf1N1B4_3841 0.62 +0.0 4,258,918 + Pf1N1B4_3841 0.62 -0.2 4,258,918 + Pf1N1B4_3841 0.62 +1.2 4,258,918 + Pf1N1B4_3841 0.62 +2.0 4,258,918 + Pf1N1B4_3841 0.62 +0.5 4,258,919 - Pf1N1B4_3841 0.62 +0.6 4,258,919 - Pf1N1B4_3841 0.62 +0.7 4,259,237 - Pf1N1B4_3841 0.88 +1.8 4,259,268 + -0.1 4,259,268 + -3.4 4,259,511 + -0.2 4,259,511 + +0.6 4,259,512 - -0.3 4,259,512 - -0.3 4,259,612 + +0.0 4,259,612 + +0.6 4,259,613 - +1.6
Or see this region's nucleotide sequence