Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3820 and Pf1N1B4_3821 are separated by 205 nucleotides Pf1N1B4_3821 and Pf1N1B4_3822 are separated by 51 nucleotides
Pf1N1B4_3820 - 2-methylcitrate synthase (EC 2.3.3.5), at 4,231,337 to 4,232,464
_3820
Pf1N1B4_3821 - 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79), at 4,232,670 to 4,235,264
_3821
Pf1N1B4_3822 - 2-methylaconitate cis-trans isomerase, at 4,235,316 to 4,236,506
_3822
Position (kb)
4232
4233
4234
4235
4236 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4231.743 kb on - strand, within Pf1N1B4_3820 at 4231.955 kb on + strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.128 kb on + strand, within Pf1N1B4_3820 at 4232.129 kb on - strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.292 kb on - strand, within Pf1N1B4_3820 at 4232.417 kb on + strand at 4232.417 kb on + strand at 4232.417 kb on + strand at 4232.418 kb on - strand at 4232.418 kb on - strand at 4232.418 kb on - strand at 4232.619 kb on + strand at 4232.620 kb on - strand at 4232.620 kb on - strand at 4232.646 kb on - strand at 4232.902 kb on + strand at 4232.903 kb on - strand at 4233.660 kb on + strand at 4233.709 kb on - strand, within Pf1N1B4_3821 at 4234.155 kb on + strand, within Pf1N1B4_3821 at 4234.297 kb on + strand at 4234.297 kb on + strand, within Pf1N1B4_3821 at 4234.298 kb on - strand, within Pf1N1B4_3821 at 4234.410 kb on + strand at 4234.410 kb on + strand, within Pf1N1B4_3821 at 4234.410 kb on + strand, within Pf1N1B4_3821 at 4234.411 kb on - strand, within Pf1N1B4_3821 at 4234.464 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand at 4234.489 kb on - strand, within Pf1N1B4_3821 at 4234.489 kb on - strand, within Pf1N1B4_3821 at 4234.489 kb on - strand, within Pf1N1B4_3821 at 4234.680 kb on + strand, within Pf1N1B4_3821 at 4234.680 kb on + strand, within Pf1N1B4_3821 at 4234.681 kb on - strand, within Pf1N1B4_3821 at 4234.845 kb on + strand, within Pf1N1B4_3821 at 4234.846 kb on - strand, within Pf1N1B4_3821 at 4234.846 kb on - strand, within Pf1N1B4_3821 at 4235.073 kb on + strand at 4235.196 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.314 kb on - strand at 4235.314 kb on - strand at 4235.352 kb on + strand at 4235.352 kb on + strand at 4235.352 kb on + strand at 4235.352 kb on + strand at 4235.352 kb on + strand at 4235.353 kb on - strand at 4235.353 kb on - strand at 4235.761 kb on + strand, within Pf1N1B4_3822 at 4236.177 kb on + strand, within Pf1N1B4_3822
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 4,231,743 - Pf1N1B4_3820 0.36 -1.8 4,231,955 + Pf1N1B4_3820 0.55 -2.1 4,231,956 - Pf1N1B4_3820 0.55 -2.1 4,231,956 - Pf1N1B4_3820 0.55 -2.2 4,231,956 - Pf1N1B4_3820 0.55 -0.4 4,232,126 + Pf1N1B4_3820 0.70 -2.3 4,232,126 + Pf1N1B4_3820 0.70 -1.9 4,232,126 + Pf1N1B4_3820 0.70 -1.9 4,232,126 + Pf1N1B4_3820 0.70 -1.7 4,232,126 + Pf1N1B4_3820 0.70 -0.4 4,232,126 + Pf1N1B4_3820 0.70 -1.5 4,232,126 + -0.4 4,232,126 + Pf1N1B4_3820 0.70 -2.2 4,232,126 + Pf1N1B4_3820 0.70 -2.6 4,232,127 - Pf1N1B4_3820 0.70 -1.7 4,232,127 - Pf1N1B4_3820 0.70 -2.6 4,232,127 - Pf1N1B4_3820 0.70 +0.1 4,232,127 - Pf1N1B4_3820 0.70 -0.2 4,232,127 - -1.2 4,232,127 - Pf1N1B4_3820 0.70 -0.6 4,232,128 + Pf1N1B4_3820 0.70 +0.6 4,232,129 - Pf1N1B4_3820 0.70 +1.0 4,232,276 + Pf1N1B4_3820 0.83 +0.1 4,232,276 + Pf1N1B4_3820 0.83 -3.0 4,232,276 + Pf1N1B4_3820 0.83 -1.4 4,232,276 + Pf1N1B4_3820 0.83 -0.1 4,232,276 + Pf1N1B4_3820 0.83 -1.4 4,232,276 + Pf1N1B4_3820 0.83 -5.0 4,232,276 + Pf1N1B4_3820 0.83 -0.5 4,232,276 + Pf1N1B4_3820 0.83 -0.7 4,232,277 - Pf1N1B4_3820 0.83 -1.7 4,232,277 - Pf1N1B4_3820 0.83 -1.7 4,232,277 - Pf1N1B4_3820 0.83 -2.1 4,232,292 - Pf1N1B4_3820 0.85 -1.8 4,232,417 + -2.3 4,232,417 + -1.6 4,232,417 + -3.6 4,232,418 - -2.1 4,232,418 - -1.3 4,232,418 - -1.2 4,232,619 + +0.2 4,232,620 - +0.0 4,232,620 - +0.0 4,232,646 - +1.2 4,232,902 + +0.3 4,232,903 - +0.1 4,233,660 + +2.2 4,233,709 - Pf1N1B4_3821 0.40 +0.6 4,234,155 + Pf1N1B4_3821 0.57 -0.9 4,234,297 + +1.0 4,234,297 + Pf1N1B4_3821 0.63 +0.0 4,234,298 - Pf1N1B4_3821 0.63 +1.1 4,234,410 + +1.6 4,234,410 + Pf1N1B4_3821 0.67 -0.3 4,234,410 + Pf1N1B4_3821 0.67 +2.2 4,234,411 - Pf1N1B4_3821 0.67 -0.4 4,234,464 + Pf1N1B4_3821 0.69 -0.7 4,234,488 + Pf1N1B4_3821 0.70 +3.6 4,234,488 + Pf1N1B4_3821 0.70 +0.2 4,234,488 + Pf1N1B4_3821 0.70 +2.4 4,234,488 + Pf1N1B4_3821 0.70 +0.9 4,234,488 + Pf1N1B4_3821 0.70 -0.2 4,234,488 + Pf1N1B4_3821 0.70 -0.1 4,234,488 + +1.6 4,234,489 - Pf1N1B4_3821 0.70 -1.0 4,234,489 - Pf1N1B4_3821 0.70 +0.3 4,234,489 - Pf1N1B4_3821 0.70 +1.1 4,234,680 + Pf1N1B4_3821 0.77 +0.0 4,234,680 + Pf1N1B4_3821 0.77 -0.2 4,234,681 - Pf1N1B4_3821 0.77 -0.4 4,234,845 + Pf1N1B4_3821 0.84 +0.6 4,234,846 - Pf1N1B4_3821 0.84 +0.0 4,234,846 - Pf1N1B4_3821 0.84 -1.9 4,235,073 + -1.0 4,235,196 + +0.0 4,235,313 + +0.6 4,235,313 + -1.4 4,235,313 + -0.7 4,235,313 + -0.7 4,235,313 + -0.2 4,235,313 + +1.0 4,235,313 + +1.2 4,235,313 + -0.2 4,235,313 + -2.0 4,235,314 - +0.0 4,235,314 - -0.1 4,235,352 + -2.0 4,235,352 + +0.6 4,235,352 + -1.2 4,235,352 + +0.3 4,235,352 + +0.9 4,235,353 - -0.4 4,235,353 - +1.6 4,235,761 + Pf1N1B4_3822 0.37 -2.6 4,236,177 + Pf1N1B4_3822 0.72 -1.0
Or see this region's nucleotide sequence