Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3682 and Pf1N1B4_3683 are separated by 110 nucleotides Pf1N1B4_3683 and Pf1N1B4_3684 are separated by 194 nucleotides
Pf1N1B4_3682 - Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61), at 4,091,097 to 4,092,314
_3682
Pf1N1B4_3683 - Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4), at 4,092,425 to 4,093,861
_3683
Pf1N1B4_3684 - Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5), at 4,094,056 to 4,095,222
_3684
Position (kb)
4092
4093
4094 Strain fitness (log2 ratio)
-2
-1
0
1 at 4093.867 kb on + strand at 4093.867 kb on + strand at 4093.867 kb on + strand at 4093.867 kb on + strand at 4093.867 kb on + strand at 4093.867 kb on + strand at 4093.867 kb on + strand at 4093.948 kb on + strand at 4093.973 kb on + strand at 4093.973 kb on + strand at 4094.033 kb on - strand at 4094.033 kb on - strand at 4094.036 kb on + strand at 4094.051 kb on + strand at 4094.051 kb on + strand at 4094.051 kb on + strand at 4094.051 kb on + strand at 4094.052 kb on - strand at 4094.052 kb on - strand at 4094.052 kb on - strand at 4094.072 kb on - strand at 4094.129 kb on + strand at 4094.129 kb on + strand at 4094.234 kb on + strand, within Pf1N1B4_3684 at 4094.305 kb on + strand at 4094.371 kb on - strand, within Pf1N1B4_3684 at 4094.380 kb on + strand at 4094.381 kb on - strand, within Pf1N1B4_3684 at 4094.600 kb on + strand at 4094.725 kb on + strand, within Pf1N1B4_3684 at 4094.737 kb on - strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.809 kb on + strand, within Pf1N1B4_3684 at 4094.810 kb on - strand, within Pf1N1B4_3684 at 4094.810 kb on - strand, within Pf1N1B4_3684 at 4094.810 kb on - strand, within Pf1N1B4_3684 at 4094.810 kb on - strand, within Pf1N1B4_3684 at 4094.810 kb on - strand at 4094.810 kb on - strand, within Pf1N1B4_3684 at 4094.813 kb on + strand, within Pf1N1B4_3684 at 4094.813 kb on + strand, within Pf1N1B4_3684 at 4094.813 kb on + strand, within Pf1N1B4_3684 at 4094.813 kb on + strand at 4094.813 kb on + strand, within Pf1N1B4_3684 at 4094.814 kb on - strand, within Pf1N1B4_3684 at 4094.814 kb on - strand, within Pf1N1B4_3684 at 4094.814 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 4,093,867 + -0.6 4,093,867 + +0.2 4,093,867 + -0.3 4,093,867 + -0.7 4,093,867 + +1.3 4,093,867 + -1.0 4,093,867 + +0.1 4,093,948 + -0.0 4,093,973 + -1.5 4,093,973 + +1.5 4,094,033 - -0.0 4,094,033 - +0.5 4,094,036 + -0.8 4,094,051 + -0.5 4,094,051 + -0.7 4,094,051 + +0.5 4,094,051 + +0.5 4,094,052 - +1.5 4,094,052 - -0.3 4,094,052 - +0.5 4,094,072 - +0.8 4,094,129 + -0.3 4,094,129 + -2.5 4,094,234 + Pf1N1B4_3684 0.15 -0.0 4,094,305 + +1.3 4,094,371 - Pf1N1B4_3684 0.27 +0.3 4,094,380 + +1.5 4,094,381 - Pf1N1B4_3684 0.28 -0.5 4,094,600 + +1.5 4,094,725 + Pf1N1B4_3684 0.57 +1.2 4,094,737 - Pf1N1B4_3684 0.58 -1.2 4,094,809 + Pf1N1B4_3684 0.65 -1.0 4,094,809 + Pf1N1B4_3684 0.65 +1.1 4,094,809 + Pf1N1B4_3684 0.65 +0.5 4,094,809 + Pf1N1B4_3684 0.65 +1.5 4,094,809 + Pf1N1B4_3684 0.65 -1.0 4,094,809 + +1.5 4,094,809 + Pf1N1B4_3684 0.65 -0.0 4,094,809 + Pf1N1B4_3684 0.65 +0.5 4,094,810 - Pf1N1B4_3684 0.65 -0.9 4,094,810 - Pf1N1B4_3684 0.65 -0.8 4,094,810 - Pf1N1B4_3684 0.65 -1.8 4,094,810 - Pf1N1B4_3684 0.65 -0.6 4,094,810 - -1.8 4,094,810 - Pf1N1B4_3684 0.65 -2.0 4,094,813 + Pf1N1B4_3684 0.65 -0.8 4,094,813 + Pf1N1B4_3684 0.65 +0.1 4,094,813 + Pf1N1B4_3684 0.65 +1.1 4,094,813 + -1.0 4,094,813 + Pf1N1B4_3684 0.65 +0.3 4,094,814 - Pf1N1B4_3684 0.65 -2.0 4,094,814 - Pf1N1B4_3684 0.65 +0.5 4,094,814 - +1.5
Or see this region's nucleotide sequence