Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3677 and Pf1N1B4_3678 overlap by 7 nucleotides Pf1N1B4_3678 and Pf1N1B4_3679 are separated by 3 nucleotides Pf1N1B4_3679 and Pf1N1B4_3680 are separated by 11 nucleotides Pf1N1B4_3680 and Pf1N1B4_3681 are separated by 276 nucleotides
Pf1N1B4_3677 - Succinate dehydrogenase cytochrome b-556 subunit, at 4,084,835 to 4,085,092
_3677
Pf1N1B4_3678 - Succinate dehydrogenase hydrophobic membrane anchor protein, at 4,085,086 to 4,085,454
_3678
Pf1N1B4_3679 - Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1), at 4,085,458 to 4,087,230
_3679
Pf1N1B4_3680 - Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1), at 4,087,242 to 4,087,946
_3680
Pf1N1B4_3681 - 2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2), at 4,088,223 to 4,091,054
_3681
Position (kb)
4085
4086
4087
4088 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4084.506 kb on - strand at 4084.515 kb on - strand at 4084.515 kb on - strand at 4084.515 kb on - strand at 4084.515 kb on - strand at 4084.515 kb on - strand at 4084.535 kb on + strand at 4084.535 kb on + strand at 4084.536 kb on - strand at 4084.536 kb on - strand at 4084.536 kb on - strand at 4084.536 kb on - strand at 4084.536 kb on - strand at 4084.563 kb on + strand at 4084.563 kb on + strand at 4084.571 kb on + strand at 4084.579 kb on + strand at 4084.579 kb on + strand at 4084.579 kb on + strand at 4084.579 kb on + strand at 4084.579 kb on + strand at 4087.972 kb on + strand at 4087.981 kb on + strand at 4087.995 kb on + strand at 4087.995 kb on + strand at 4087.995 kb on + strand at 4087.995 kb on + strand at 4087.995 kb on + strand at 4088.034 kb on + strand at 4088.051 kb on + strand at 4088.051 kb on + strand at 4088.051 kb on + strand at 4088.051 kb on + strand at 4088.051 kb on + strand at 4088.051 kb on + strand at 4088.053 kb on + strand at 4088.053 kb on + strand at 4088.053 kb on + strand at 4088.053 kb on + strand at 4088.053 kb on + strand at 4088.053 kb on + strand at 4088.053 kb on + strand at 4088.068 kb on + strand at 4088.068 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 4,084,506 - +0.3 4,084,515 - +1.5 4,084,515 - -1.6 4,084,515 - -2.1 4,084,515 - -1.0 4,084,515 - -2.3 4,084,535 + +3.3 4,084,535 + -1.4 4,084,536 - +0.5 4,084,536 - -3.2 4,084,536 - +1.0 4,084,536 - -0.8 4,084,536 - -0.3 4,084,563 + +1.5 4,084,563 + +0.7 4,084,571 + +1.5 4,084,579 + +0.5 4,084,579 + -0.5 4,084,579 + -0.3 4,084,579 + -0.7 4,084,579 + +1.9 4,087,972 + +1.1 4,087,981 + -0.0 4,087,995 + +0.3 4,087,995 + -2.0 4,087,995 + -1.0 4,087,995 + +1.0 4,087,995 + -0.6 4,088,034 + +0.5 4,088,051 + +0.8 4,088,051 + +0.8 4,088,051 + +2.9 4,088,051 + -1.3 4,088,051 + -0.0 4,088,051 + +0.2 4,088,053 + +0.7 4,088,053 + -0.3 4,088,053 + +0.5 4,088,053 + -0.6 4,088,053 + +0.7 4,088,053 + +1.4 4,088,053 + +2.0 4,088,068 + +1.5 4,088,068 + -0.1
Or see this region's nucleotide sequence