Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3605 and Pf1N1B4_3606 are separated by 7 nucleotides Pf1N1B4_3606 and Pf1N1B4_3607 overlap by 11 nucleotides Pf1N1B4_3607 and Pf1N1B4_3608 are separated by 6 nucleotides Pf1N1B4_3608 and Pf1N1B4_3609 are separated by 218 nucleotides Pf1N1B4_3609 and Pf1N1B4_3610 are separated by 5 nucleotides
Pf1N1B4_3605 - Flagellar motor switch protein FliG, at 4,016,247 to 4,017,266
_3605
Pf1N1B4_3606 - Flagellar assembly protein FliH, at 4,017,274 to 4,018,083
_3606
Pf1N1B4_3607 - Flagellum-specific ATP synthase FliI, at 4,018,073 to 4,019,431
_3607
Pf1N1B4_3608 - Flagellar protein FliJ, at 4,019,438 to 4,019,887
_3608
Pf1N1B4_3609 - Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV, at 4,020,106 to 4,020,411
_3609
Pf1N1B4_3610 - Serine phosphatase RsbU, regulator of sigma subunit, at 4,020,417 to 4,022,120
_3610
Position (kb)
4018
4019
4020 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4017.209 kb on + strand at 4017.264 kb on + strand at 4017.264 kb on + strand at 4017.264 kb on + strand at 4017.264 kb on + strand at 4017.264 kb on + strand at 4017.265 kb on - strand at 4017.265 kb on - strand at 4017.265 kb on - strand at 4017.265 kb on - strand at 4017.265 kb on - strand at 4017.273 kb on + strand at 4017.274 kb on - strand at 4017.274 kb on - strand at 4017.274 kb on - strand at 4017.274 kb on - strand at 4017.484 kb on + strand, within Pf1N1B4_3606 at 4017.484 kb on + strand, within Pf1N1B4_3606 at 4017.484 kb on + strand, within Pf1N1B4_3606 at 4017.485 kb on - strand, within Pf1N1B4_3606 at 4017.485 kb on - strand at 4017.784 kb on + strand, within Pf1N1B4_3606 at 4017.784 kb on + strand, within Pf1N1B4_3606 at 4017.784 kb on + strand, within Pf1N1B4_3606 at 4017.784 kb on + strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4018.042 kb on - strand at 4018.115 kb on + strand at 4018.250 kb on + strand, within Pf1N1B4_3607 at 4018.251 kb on - strand, within Pf1N1B4_3607 at 4018.805 kb on + strand at 4018.805 kb on + strand, within Pf1N1B4_3607 at 4018.806 kb on - strand, within Pf1N1B4_3607 at 4018.826 kb on + strand, within Pf1N1B4_3607 at 4018.827 kb on - strand, within Pf1N1B4_3607 at 4019.035 kb on - strand, within Pf1N1B4_3607 at 4019.231 kb on + strand, within Pf1N1B4_3607 at 4019.231 kb on + strand, within Pf1N1B4_3607 at 4019.279 kb on + strand, within Pf1N1B4_3607 at 4019.279 kb on + strand, within Pf1N1B4_3607 at 4019.280 kb on - strand, within Pf1N1B4_3607 at 4019.280 kb on - strand, within Pf1N1B4_3607 at 4019.342 kb on + strand at 4019.342 kb on + strand at 4019.342 kb on + strand at 4019.342 kb on + strand at 4019.343 kb on - strand at 4019.343 kb on - strand at 4019.343 kb on - strand at 4019.343 kb on - strand at 4019.343 kb on - strand at 4019.343 kb on - strand at 4019.343 kb on - strand at 4019.343 kb on - strand at 4019.589 kb on - strand, within Pf1N1B4_3608 at 4019.719 kb on + strand, within Pf1N1B4_3608 at 4019.720 kb on - strand, within Pf1N1B4_3608 at 4019.882 kb on + strand at 4019.934 kb on - strand at 4019.980 kb on - strand at 4020.066 kb on + strand at 4020.066 kb on + strand at 4020.066 kb on + strand at 4020.067 kb on - strand at 4020.067 kb on - strand at 4020.067 kb on - strand at 4020.072 kb on + strand at 4020.072 kb on + strand at 4020.072 kb on + strand at 4020.073 kb on - strand at 4020.073 kb on - strand at 4020.073 kb on - strand at 4020.073 kb on - strand at 4020.073 kb on - strand at 4020.125 kb on + strand at 4020.126 kb on - strand at 4020.126 kb on - strand at 4020.126 kb on - strand at 4020.126 kb on - strand at 4020.126 kb on - strand at 4020.205 kb on + strand, within Pf1N1B4_3609 at 4020.206 kb on - strand, within Pf1N1B4_3609 at 4020.206 kb on - strand, within Pf1N1B4_3609 at 4020.206 kb on - strand, within Pf1N1B4_3609 at 4020.262 kb on + strand, within Pf1N1B4_3609 at 4020.262 kb on + strand, within Pf1N1B4_3609 at 4020.263 kb on - strand, within Pf1N1B4_3609 at 4020.263 kb on - strand, within Pf1N1B4_3609 at 4020.263 kb on - strand, within Pf1N1B4_3609 at 4020.263 kb on - strand, within Pf1N1B4_3609
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 4,017,209 + +0.8 4,017,264 + +0.8 4,017,264 + +0.4 4,017,264 + +0.2 4,017,264 + -0.3 4,017,264 + +0.7 4,017,265 - -2.5 4,017,265 - +0.4 4,017,265 - +0.4 4,017,265 - +0.4 4,017,265 - +1.8 4,017,273 + +0.9 4,017,274 - -1.1 4,017,274 - +1.5 4,017,274 - -1.6 4,017,274 - -0.4 4,017,484 + Pf1N1B4_3606 0.26 -0.6 4,017,484 + Pf1N1B4_3606 0.26 +0.9 4,017,484 + Pf1N1B4_3606 0.26 +0.1 4,017,485 - Pf1N1B4_3606 0.26 +0.0 4,017,485 - -0.1 4,017,784 + Pf1N1B4_3606 0.63 -0.5 4,017,784 + Pf1N1B4_3606 0.63 -0.1 4,017,784 + Pf1N1B4_3606 0.63 -0.2 4,017,784 + Pf1N1B4_3606 0.63 +0.4 4,017,785 - Pf1N1B4_3606 0.63 +0.1 4,017,785 - Pf1N1B4_3606 0.63 +0.7 4,017,785 - Pf1N1B4_3606 0.63 +0.2 4,017,785 - Pf1N1B4_3606 0.63 -1.3 4,017,785 - Pf1N1B4_3606 0.63 +1.7 4,017,785 - Pf1N1B4_3606 0.63 -0.1 4,017,785 - Pf1N1B4_3606 0.63 -1.1 4,017,785 - Pf1N1B4_3606 0.63 -0.6 4,017,785 - Pf1N1B4_3606 0.63 -1.1 4,017,785 - Pf1N1B4_3606 0.63 -0.1 4,018,042 - -1.1 4,018,115 + +1.6 4,018,250 + Pf1N1B4_3607 0.13 +1.1 4,018,251 - Pf1N1B4_3607 0.13 +0.3 4,018,805 + -0.9 4,018,805 + Pf1N1B4_3607 0.54 +0.3 4,018,806 - Pf1N1B4_3607 0.54 +0.0 4,018,826 + Pf1N1B4_3607 0.55 +2.6 4,018,827 - Pf1N1B4_3607 0.55 -0.1 4,019,035 - Pf1N1B4_3607 0.71 -2.2 4,019,231 + Pf1N1B4_3607 0.85 -0.1 4,019,231 + Pf1N1B4_3607 0.85 -0.8 4,019,279 + Pf1N1B4_3607 0.89 +0.6 4,019,279 + Pf1N1B4_3607 0.89 +0.4 4,019,280 - Pf1N1B4_3607 0.89 +1.9 4,019,280 - Pf1N1B4_3607 0.89 +0.1 4,019,342 + -0.5 4,019,342 + -1.7 4,019,342 + +0.3 4,019,342 + -1.1 4,019,343 - +1.8 4,019,343 - -0.1 4,019,343 - +0.7 4,019,343 - +0.3 4,019,343 - -0.8 4,019,343 - -1.4 4,019,343 - -0.7 4,019,343 - +2.6 4,019,589 - Pf1N1B4_3608 0.34 -0.8 4,019,719 + Pf1N1B4_3608 0.62 -0.7 4,019,720 - Pf1N1B4_3608 0.63 -0.5 4,019,882 + +0.4 4,019,934 - +2.7 4,019,980 - -1.1 4,020,066 + -2.1 4,020,066 + -1.4 4,020,066 + -1.5 4,020,067 - -0.1 4,020,067 - +0.0 4,020,067 - -2.9 4,020,072 + +0.9 4,020,072 + +0.1 4,020,072 + +1.5 4,020,073 - -0.3 4,020,073 - +0.5 4,020,073 - -0.9 4,020,073 - +0.6 4,020,073 - -0.5 4,020,125 + -2.1 4,020,126 - -1.5 4,020,126 - +2.5 4,020,126 - +0.2 4,020,126 - -1.0 4,020,126 - +0.5 4,020,205 + Pf1N1B4_3609 0.32 -1.8 4,020,206 - Pf1N1B4_3609 0.33 -1.8 4,020,206 - Pf1N1B4_3609 0.33 -0.5 4,020,206 - Pf1N1B4_3609 0.33 -0.3 4,020,262 + Pf1N1B4_3609 0.51 -0.4 4,020,262 + Pf1N1B4_3609 0.51 -0.4 4,020,263 - Pf1N1B4_3609 0.51 -0.4 4,020,263 - Pf1N1B4_3609 0.51 +0.1 4,020,263 - Pf1N1B4_3609 0.51 -0.4 4,020,263 - Pf1N1B4_3609 0.51 +0.5
Or see this region's nucleotide sequence