Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_358 and Pf1N1B4_359 are separated by 374 nucleotides Pf1N1B4_359 and Pf1N1B4_360 are separated by 140 nucleotides Pf1N1B4_360 and Pf1N1B4_361 are separated by 44 nucleotides
Pf1N1B4_358 - Oxidoreductase, short chain dehydrogenase/reductase family, at 424,920 to 425,660
_358
Pf1N1B4_359 - GGDEF domain protein, at 426,035 to 426,961
_359
Pf1N1B4_360 - Pyrroloquinoline quinone (Coenzyme PQQ) biosynthesis protein C, at 427,102 to 427,896
_360
Pf1N1B4_361 - Sensory box/GGDEF family protein, at 427,941 to 430,400
_361
Position (kb)
426
427 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand at 425.157 kb on + strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.544 kb on + strand, within Pf1N1B4_358 at 425.598 kb on + strand at 425.598 kb on + strand at 425.598 kb on + strand at 425.599 kb on - strand at 425.599 kb on - strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.605 kb on - strand at 425.605 kb on - strand at 425.658 kb on + strand at 425.721 kb on - strand at 425.745 kb on - strand at 425.745 kb on - strand at 425.745 kb on - strand at 425.905 kb on + strand at 425.905 kb on + strand at 425.906 kb on - strand at 425.925 kb on + strand at 425.925 kb on + strand at 426.001 kb on + strand at 426.012 kb on + strand at 426.013 kb on - strand at 426.211 kb on - strand, within Pf1N1B4_359 at 426.317 kb on + strand, within Pf1N1B4_359 at 426.620 kb on + strand, within Pf1N1B4_359 at 426.620 kb on + strand, within Pf1N1B4_359 at 426.620 kb on + strand, within Pf1N1B4_359 at 426.621 kb on - strand, within Pf1N1B4_359 at 426.807 kb on - strand, within Pf1N1B4_359 at 426.807 kb on - strand, within Pf1N1B4_359 at 426.914 kb on + strand at 426.914 kb on + strand at 426.914 kb on + strand at 426.914 kb on + strand at 426.914 kb on + strand at 426.914 kb on + strand at 426.914 kb on + strand at 426.914 kb on + strand at 426.915 kb on - strand at 426.915 kb on - strand at 426.978 kb on + strand at 426.978 kb on + strand at 426.978 kb on + strand at 426.978 kb on + strand at 426.978 kb on + strand at 426.979 kb on - strand at 426.980 kb on + strand at 426.981 kb on - strand at 427.019 kb on + strand at 427.019 kb on + strand at 427.187 kb on + strand, within Pf1N1B4_360 at 427.187 kb on + strand, within Pf1N1B4_360 at 427.187 kb on + strand, within Pf1N1B4_360 at 427.187 kb on + strand, within Pf1N1B4_360 at 427.187 kb on + strand, within Pf1N1B4_360 at 427.187 kb on + strand, within Pf1N1B4_360 at 427.187 kb on + strand, within Pf1N1B4_360 at 427.187 kb on + strand, within Pf1N1B4_360 at 427.187 kb on + strand, within Pf1N1B4_360 at 427.187 kb on + strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.188 kb on - strand, within Pf1N1B4_360 at 427.193 kb on + strand, within Pf1N1B4_360 at 427.193 kb on + strand, within Pf1N1B4_360 at 427.193 kb on + strand, within Pf1N1B4_360 at 427.194 kb on - strand, within Pf1N1B4_360 at 427.194 kb on - strand, within Pf1N1B4_360 at 427.194 kb on - strand, within Pf1N1B4_360 at 427.194 kb on - strand, within Pf1N1B4_360 at 427.194 kb on - strand, within Pf1N1B4_360 at 427.194 kb on - strand, within Pf1N1B4_360 at 427.194 kb on - strand, within Pf1N1B4_360 at 427.373 kb on + strand at 427.373 kb on + strand, within Pf1N1B4_360 at 427.374 kb on - strand, within Pf1N1B4_360 at 427.374 kb on - strand, within Pf1N1B4_360 at 427.430 kb on + strand, within Pf1N1B4_360 at 427.430 kb on + strand, within Pf1N1B4_360 at 427.431 kb on - strand, within Pf1N1B4_360 at 427.431 kb on - strand, within Pf1N1B4_360 at 427.431 kb on - strand, within Pf1N1B4_360 at 427.431 kb on - strand, within Pf1N1B4_360 at 427.431 kb on - strand, within Pf1N1B4_360 at 427.431 kb on - strand, within Pf1N1B4_360 at 427.431 kb on - strand, within Pf1N1B4_360 at 427.431 kb on - strand, within Pf1N1B4_360 at 427.683 kb on + strand, within Pf1N1B4_360 at 427.684 kb on - strand, within Pf1N1B4_360 at 427.684 kb on - strand, within Pf1N1B4_360 at 427.684 kb on - strand, within Pf1N1B4_360 at 427.684 kb on - strand, within Pf1N1B4_360 at 427.684 kb on - strand, within Pf1N1B4_360 at 427.684 kb on - strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.685 kb on + strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.686 kb on - strand, within Pf1N1B4_360 at 427.687 kb on + strand, within Pf1N1B4_360 at 427.687 kb on + strand, within Pf1N1B4_360 at 427.688 kb on - strand, within Pf1N1B4_360 at 427.688 kb on - strand, within Pf1N1B4_360 at 427.688 kb on - strand, within Pf1N1B4_360 at 427.688 kb on - strand, within Pf1N1B4_360 at 427.688 kb on - strand, within Pf1N1B4_360 at 427.688 kb on - strand, within Pf1N1B4_360 at 427.688 kb on - strand, within Pf1N1B4_360 at 427.688 kb on - strand, within Pf1N1B4_360 at 427.688 kb on - strand, within Pf1N1B4_360 at 427.688 kb on - strand, within Pf1N1B4_360 at 427.723 kb on - strand, within Pf1N1B4_360 at 427.723 kb on - strand, within Pf1N1B4_360 at 427.723 kb on - strand, within Pf1N1B4_360 at 427.727 kb on + strand, within Pf1N1B4_360 at 427.727 kb on + strand, within Pf1N1B4_360 at 427.728 kb on - strand, within Pf1N1B4_360 at 427.728 kb on - strand, within Pf1N1B4_360 at 427.758 kb on + strand, within Pf1N1B4_360 at 427.758 kb on + strand, within Pf1N1B4_360 at 427.759 kb on - strand, within Pf1N1B4_360 at 427.759 kb on - strand, within Pf1N1B4_360 at 427.775 kb on + strand, within Pf1N1B4_360 at 427.776 kb on - strand, within Pf1N1B4_360 at 427.848 kb on - strand at 427.888 kb on - strand at 427.924 kb on - strand at 427.924 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 425,079 + Pf1N1B4_358 0.21 +0.3 425,079 + Pf1N1B4_358 0.21 -1.8 425,079 + Pf1N1B4_358 0.21 -0.6 425,079 + Pf1N1B4_358 0.21 -1.5 425,080 - Pf1N1B4_358 0.22 +0.6 425,080 - Pf1N1B4_358 0.22 -1.6 425,080 - Pf1N1B4_358 0.22 +1.6 425,080 - Pf1N1B4_358 0.22 +0.5 425,157 + Pf1N1B4_358 0.32 +0.6 425,157 + Pf1N1B4_358 0.32 -0.0 425,157 + Pf1N1B4_358 0.32 -0.0 425,157 + Pf1N1B4_358 0.32 -0.8 425,157 + Pf1N1B4_358 0.32 +0.1 425,157 + -0.6 425,157 + Pf1N1B4_358 0.32 -0.4 425,158 - Pf1N1B4_358 0.32 -0.3 425,158 - Pf1N1B4_358 0.32 -0.0 425,158 - Pf1N1B4_358 0.32 -0.8 425,158 - Pf1N1B4_358 0.32 +0.6 425,158 - Pf1N1B4_358 0.32 -1.4 425,470 + Pf1N1B4_358 0.74 +0.6 425,470 + Pf1N1B4_358 0.74 -1.6 425,470 + Pf1N1B4_358 0.74 +1.3 425,470 + Pf1N1B4_358 0.74 -0.0 425,470 + Pf1N1B4_358 0.74 -1.0 425,470 + Pf1N1B4_358 0.74 -2.0 425,471 - Pf1N1B4_358 0.74 -0.0 425,471 - Pf1N1B4_358 0.74 -2.0 425,471 - Pf1N1B4_358 0.74 -0.0 425,471 - Pf1N1B4_358 0.74 -0.7 425,471 - Pf1N1B4_358 0.74 -1.4 425,471 - Pf1N1B4_358 0.74 -1.8 425,471 - Pf1N1B4_358 0.74 -0.8 425,544 + Pf1N1B4_358 0.84 +1.1 425,598 + -0.2 425,598 + -0.2 425,598 + -1.6 425,599 - -0.4 425,599 - -1.8 425,604 + +0.6 425,604 + -0.4 425,604 + -1.6 425,604 + -0.0 425,604 + +0.6 425,604 + -0.0 425,605 - -1.3 425,605 - -1.9 425,658 + +0.6 425,721 - -0.9 425,745 - +0.5 425,745 - -1.3 425,745 - +2.3 425,905 + +1.6 425,905 + +0.2 425,906 - +1.6 425,925 + +1.7 425,925 + -1.4 426,001 + +1.0 426,012 + +0.7 426,013 - +0.6 426,211 - Pf1N1B4_359 0.19 -0.0 426,317 + Pf1N1B4_359 0.30 +0.1 426,620 + Pf1N1B4_359 0.63 -1.5 426,620 + Pf1N1B4_359 0.63 +1.1 426,620 + Pf1N1B4_359 0.63 +0.9 426,621 - Pf1N1B4_359 0.63 +1.1 426,807 - Pf1N1B4_359 0.83 +0.9 426,807 - Pf1N1B4_359 0.83 +1.3 426,914 + -0.3 426,914 + -0.0 426,914 + -1.0 426,914 + -0.9 426,914 + +0.3 426,914 + -0.1 426,914 + -0.5 426,914 + -0.0 426,915 - +0.6 426,915 - -2.0 426,978 + -0.4 426,978 + +0.6 426,978 + -1.4 426,978 + -0.8 426,978 + -1.3 426,979 - +0.4 426,980 + -0.7 426,981 - -0.6 427,019 + -0.0 427,019 + -0.3 427,187 + Pf1N1B4_360 0.11 +0.9 427,187 + Pf1N1B4_360 0.11 -2.3 427,187 + Pf1N1B4_360 0.11 +0.8 427,187 + Pf1N1B4_360 0.11 -0.1 427,187 + Pf1N1B4_360 0.11 -0.3 427,187 + Pf1N1B4_360 0.11 +0.1 427,187 + Pf1N1B4_360 0.11 +0.0 427,187 + Pf1N1B4_360 0.11 -1.6 427,187 + Pf1N1B4_360 0.11 +1.2 427,187 + Pf1N1B4_360 0.11 -1.6 427,188 - Pf1N1B4_360 0.11 +0.1 427,188 - Pf1N1B4_360 0.11 +1.0 427,188 - Pf1N1B4_360 0.11 -0.3 427,188 - Pf1N1B4_360 0.11 +1.0 427,188 - Pf1N1B4_360 0.11 -2.8 427,188 - Pf1N1B4_360 0.11 +1.3 427,188 - -0.0 427,188 - Pf1N1B4_360 0.11 +2.0 427,188 - Pf1N1B4_360 0.11 +2.4 427,188 - Pf1N1B4_360 0.11 -0.3 427,188 - Pf1N1B4_360 0.11 -2.5 427,188 - Pf1N1B4_360 0.11 -2.1 427,188 - Pf1N1B4_360 0.11 -0.9 427,188 - Pf1N1B4_360 0.11 -1.6 427,188 - Pf1N1B4_360 0.11 -0.0 427,188 - Pf1N1B4_360 0.11 +0.0 427,188 - Pf1N1B4_360 0.11 -1.8 427,188 - Pf1N1B4_360 0.11 +0.4 427,188 - Pf1N1B4_360 0.11 -0.0 427,193 + Pf1N1B4_360 0.11 +0.8 427,193 + Pf1N1B4_360 0.11 +0.6 427,193 + Pf1N1B4_360 0.11 +0.6 427,194 - Pf1N1B4_360 0.12 +1.4 427,194 - Pf1N1B4_360 0.12 +3.0 427,194 - Pf1N1B4_360 0.12 -0.8 427,194 - Pf1N1B4_360 0.12 +0.3 427,194 - Pf1N1B4_360 0.12 -0.4 427,194 - Pf1N1B4_360 0.12 -0.0 427,194 - Pf1N1B4_360 0.12 +0.6 427,373 + -0.3 427,373 + Pf1N1B4_360 0.34 -1.3 427,374 - Pf1N1B4_360 0.34 +1.6 427,374 - Pf1N1B4_360 0.34 +1.6 427,430 + Pf1N1B4_360 0.41 -1.3 427,430 + Pf1N1B4_360 0.41 -1.9 427,431 - Pf1N1B4_360 0.41 -0.2 427,431 - Pf1N1B4_360 0.41 -2.0 427,431 - Pf1N1B4_360 0.41 -1.6 427,431 - Pf1N1B4_360 0.41 +0.4 427,431 - Pf1N1B4_360 0.41 -1.3 427,431 - Pf1N1B4_360 0.41 -0.0 427,431 - Pf1N1B4_360 0.41 -1.0 427,431 - Pf1N1B4_360 0.41 +1.3 427,683 + Pf1N1B4_360 0.73 -0.8 427,684 - Pf1N1B4_360 0.73 -0.0 427,684 - Pf1N1B4_360 0.73 -0.2 427,684 - Pf1N1B4_360 0.73 +0.6 427,684 - Pf1N1B4_360 0.73 -0.4 427,684 - Pf1N1B4_360 0.73 -1.4 427,684 - Pf1N1B4_360 0.73 +1.0 427,685 + Pf1N1B4_360 0.73 -1.8 427,685 + Pf1N1B4_360 0.73 +2.8 427,685 + Pf1N1B4_360 0.73 -1.0 427,685 + Pf1N1B4_360 0.73 +0.1 427,685 + Pf1N1B4_360 0.73 -0.8 427,685 + Pf1N1B4_360 0.73 -0.6 427,685 + Pf1N1B4_360 0.73 -1.0 427,685 + -1.6 427,685 + Pf1N1B4_360 0.73 +0.3 427,685 + Pf1N1B4_360 0.73 +0.2 427,685 + Pf1N1B4_360 0.73 +1.0 427,685 + Pf1N1B4_360 0.73 +1.6 427,685 + Pf1N1B4_360 0.73 -0.7 427,685 + Pf1N1B4_360 0.73 +0.3 427,685 + Pf1N1B4_360 0.73 -0.9 427,685 + Pf1N1B4_360 0.73 -1.4 427,686 - Pf1N1B4_360 0.73 +2.6 427,686 - Pf1N1B4_360 0.73 +1.0 427,686 - Pf1N1B4_360 0.73 -2.8 427,686 - Pf1N1B4_360 0.73 -1.0 427,686 - Pf1N1B4_360 0.73 +0.8 427,686 - Pf1N1B4_360 0.73 +0.4 427,686 - Pf1N1B4_360 0.73 +0.8 427,686 - Pf1N1B4_360 0.73 -0.8 427,686 - Pf1N1B4_360 0.73 -0.7 427,686 - Pf1N1B4_360 0.73 -0.3 427,686 - Pf1N1B4_360 0.73 +1.0 427,686 - Pf1N1B4_360 0.73 +1.7 427,686 - Pf1N1B4_360 0.73 -0.4 427,686 - Pf1N1B4_360 0.73 +2.1 427,686 - Pf1N1B4_360 0.73 -2.3 427,686 - Pf1N1B4_360 0.73 -0.0 427,686 - Pf1N1B4_360 0.73 +0.1 427,686 - Pf1N1B4_360 0.73 +0.7 427,686 - Pf1N1B4_360 0.73 -1.0 427,686 - Pf1N1B4_360 0.73 -0.7 427,687 + Pf1N1B4_360 0.74 -0.4 427,687 + Pf1N1B4_360 0.74 -0.6 427,688 - Pf1N1B4_360 0.74 -2.4 427,688 - Pf1N1B4_360 0.74 +0.8 427,688 - Pf1N1B4_360 0.74 -0.3 427,688 - Pf1N1B4_360 0.74 -0.9 427,688 - Pf1N1B4_360 0.74 -1.6 427,688 - Pf1N1B4_360 0.74 +0.1 427,688 - Pf1N1B4_360 0.74 -0.0 427,688 - Pf1N1B4_360 0.74 -2.0 427,688 - Pf1N1B4_360 0.74 -0.8 427,688 - Pf1N1B4_360 0.74 -0.1 427,723 - Pf1N1B4_360 0.78 +0.6 427,723 - Pf1N1B4_360 0.78 -0.5 427,723 - Pf1N1B4_360 0.78 +0.7 427,727 + Pf1N1B4_360 0.79 +0.6 427,727 + Pf1N1B4_360 0.79 -0.0 427,728 - Pf1N1B4_360 0.79 -0.0 427,728 - Pf1N1B4_360 0.79 -0.8 427,758 + Pf1N1B4_360 0.83 +0.1 427,758 + Pf1N1B4_360 0.83 -0.0 427,759 - Pf1N1B4_360 0.83 -1.1 427,759 - Pf1N1B4_360 0.83 -0.9 427,775 + Pf1N1B4_360 0.85 -0.3 427,776 - Pf1N1B4_360 0.85 -1.6 427,848 - +0.2 427,888 - +0.6 427,924 - -1.6 427,924 - +1.3
Or see this region's nucleotide sequence