Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3183 and Pf1N1B4_3184 are separated by 145 nucleotides Pf1N1B4_3184 and Pf1N1B4_3185 are separated by 199 nucleotides Pf1N1B4_3185 and Pf1N1B4_3186 are separated by 5 nucleotides Pf1N1B4_3186 and Pf1N1B4_3187 are separated by 114 nucleotides
Pf1N1B4_3183 - Glutathione S-transferase (EC 2.5.1.18), at 3,567,569 to 3,568,213
_3183
Pf1N1B4_3184 - Isochorismatase (EC 3.3.2.1), at 3,568,359 to 3,568,916
_3184
Pf1N1B4_3185 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,569,116 to 3,569,325
_3185
Pf1N1B4_3186 - Conserved secreted protein, at 3,569,331 to 3,569,816
_3186
Pf1N1B4_3187 - UPF0225 protein YchJ, at 3,569,931 to 3,570,407
_3187
Position (kb)
3569
3570 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3568.137 kb on + strand, within Pf1N1B4_3183 at 3568.138 kb on - strand, within Pf1N1B4_3183 at 3568.197 kb on + strand at 3568.198 kb on - strand at 3568.200 kb on + strand at 3568.200 kb on + strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.245 kb on + strand at 3568.302 kb on - strand at 3568.787 kb on + strand, within Pf1N1B4_3184 at 3568.787 kb on + strand, within Pf1N1B4_3184 at 3568.788 kb on - strand, within Pf1N1B4_3184 at 3568.806 kb on + strand, within Pf1N1B4_3184 at 3568.807 kb on - strand, within Pf1N1B4_3184 at 3568.872 kb on + strand at 3568.872 kb on + strand at 3568.914 kb on + strand at 3568.954 kb on + strand at 3568.954 kb on + strand at 3569.013 kb on + strand at 3569.013 kb on + strand at 3569.025 kb on + strand at 3569.344 kb on - strand at 3569.358 kb on + strand at 3569.386 kb on + strand, within Pf1N1B4_3186 at 3569.386 kb on + strand, within Pf1N1B4_3186 at 3569.386 kb on + strand, within Pf1N1B4_3186 at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.387 kb on - strand at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.401 kb on - strand, within Pf1N1B4_3186 at 3569.401 kb on - strand, within Pf1N1B4_3186 at 3569.513 kb on + strand, within Pf1N1B4_3186 at 3569.513 kb on + strand, within Pf1N1B4_3186 at 3569.514 kb on - strand, within Pf1N1B4_3186 at 3569.519 kb on - strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.676 kb on + strand, within Pf1N1B4_3186 at 3569.676 kb on + strand, within Pf1N1B4_3186 at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.794 kb on - strand at 3569.824 kb on + strand at 3569.825 kb on - strand at 3569.845 kb on + strand at 3569.845 kb on + strand at 3569.845 kb on + strand at 3569.879 kb on + strand at 3569.879 kb on + strand at 3569.880 kb on - strand at 3569.944 kb on + strand at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.037 kb on + strand, within Pf1N1B4_3187 at 3570.037 kb on + strand, within Pf1N1B4_3187 at 3570.038 kb on - strand, within Pf1N1B4_3187 at 3570.038 kb on - strand, within Pf1N1B4_3187 at 3570.058 kb on - strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.265 kb on + strand, within Pf1N1B4_3187 at 3570.266 kb on - strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.286 kb on + strand, within Pf1N1B4_3187 at 3570.286 kb on + strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 3,568,137 + Pf1N1B4_3183 0.88 -1.0 3,568,138 - Pf1N1B4_3183 0.88 -2.3 3,568,197 + -1.5 3,568,198 - -0.0 3,568,200 + -0.2 3,568,200 + -0.3 3,568,201 - +0.6 3,568,201 - -1.4 3,568,201 - -0.9 3,568,201 - -1.0 3,568,201 - +2.6 3,568,201 - -1.4 3,568,201 - -1.9 3,568,245 + +1.1 3,568,302 - -1.0 3,568,787 + Pf1N1B4_3184 0.77 -0.5 3,568,787 + Pf1N1B4_3184 0.77 +1.1 3,568,788 - Pf1N1B4_3184 0.77 +0.3 3,568,806 + Pf1N1B4_3184 0.80 -1.0 3,568,807 - Pf1N1B4_3184 0.80 +0.1 3,568,872 + -1.0 3,568,872 + -2.2 3,568,914 + +0.4 3,568,954 + -1.0 3,568,954 + +1.7 3,569,013 + +0.1 3,569,013 + -1.6 3,569,025 + -0.6 3,569,344 - -2.3 3,569,358 + -0.9 3,569,386 + Pf1N1B4_3186 0.11 +1.9 3,569,386 + Pf1N1B4_3186 0.11 -0.6 3,569,386 + Pf1N1B4_3186 0.11 +1.0 3,569,387 - Pf1N1B4_3186 0.12 +0.1 3,569,387 - Pf1N1B4_3186 0.12 +0.2 3,569,387 - -0.8 3,569,387 - Pf1N1B4_3186 0.12 -0.2 3,569,387 - Pf1N1B4_3186 0.12 +0.5 3,569,401 - Pf1N1B4_3186 0.14 +0.2 3,569,401 - Pf1N1B4_3186 0.14 -1.8 3,569,513 + Pf1N1B4_3186 0.37 +0.6 3,569,513 + Pf1N1B4_3186 0.37 +0.2 3,569,514 - Pf1N1B4_3186 0.38 -0.9 3,569,519 - Pf1N1B4_3186 0.39 -0.8 3,569,577 + Pf1N1B4_3186 0.51 -1.9 3,569,577 + Pf1N1B4_3186 0.51 -1.0 3,569,577 + Pf1N1B4_3186 0.51 -0.5 3,569,577 + Pf1N1B4_3186 0.51 -0.2 3,569,577 + Pf1N1B4_3186 0.51 -0.9 3,569,577 + Pf1N1B4_3186 0.51 +0.3 3,569,577 + Pf1N1B4_3186 0.51 -0.3 3,569,577 + Pf1N1B4_3186 0.51 -0.2 3,569,577 + Pf1N1B4_3186 0.51 -0.2 3,569,577 + Pf1N1B4_3186 0.51 +0.5 3,569,577 + Pf1N1B4_3186 0.51 +3.0 3,569,577 + Pf1N1B4_3186 0.51 +2.5 3,569,577 + Pf1N1B4_3186 0.51 +1.2 3,569,577 + Pf1N1B4_3186 0.51 -1.4 3,569,577 + Pf1N1B4_3186 0.51 -0.5 3,569,578 - Pf1N1B4_3186 0.51 +0.4 3,569,578 - Pf1N1B4_3186 0.51 +0.0 3,569,578 - Pf1N1B4_3186 0.51 -0.6 3,569,578 - Pf1N1B4_3186 0.51 -0.7 3,569,578 - Pf1N1B4_3186 0.51 -2.0 3,569,578 - Pf1N1B4_3186 0.51 -1.0 3,569,578 - Pf1N1B4_3186 0.51 -1.1 3,569,578 - Pf1N1B4_3186 0.51 -0.6 3,569,676 + Pf1N1B4_3186 0.71 +0.6 3,569,676 + Pf1N1B4_3186 0.71 +1.4 3,569,793 + -0.5 3,569,793 + -0.5 3,569,793 + -2.5 3,569,793 + +1.2 3,569,794 - +0.2 3,569,824 + -0.3 3,569,825 - +0.9 3,569,845 + -0.3 3,569,845 + +0.6 3,569,845 + +1.3 3,569,879 + -1.3 3,569,879 + +0.4 3,569,880 - +0.2 3,569,944 + +0.5 3,570,000 - Pf1N1B4_3187 0.14 -0.1 3,570,000 - Pf1N1B4_3187 0.14 +0.3 3,570,000 - Pf1N1B4_3187 0.14 +0.8 3,570,037 + Pf1N1B4_3187 0.22 +0.7 3,570,037 + Pf1N1B4_3187 0.22 -2.7 3,570,038 - Pf1N1B4_3187 0.22 -1.2 3,570,038 - Pf1N1B4_3187 0.22 +1.0 3,570,058 - Pf1N1B4_3187 0.27 +1.0 3,570,261 + Pf1N1B4_3187 0.69 +1.3 3,570,261 + Pf1N1B4_3187 0.69 +1.1 3,570,261 + Pf1N1B4_3187 0.69 +0.4 3,570,261 + Pf1N1B4_3187 0.69 -1.2 3,570,262 - Pf1N1B4_3187 0.69 -0.9 3,570,262 - Pf1N1B4_3187 0.69 -0.1 3,570,262 - Pf1N1B4_3187 0.69 +1.1 3,570,262 - Pf1N1B4_3187 0.69 +1.8 3,570,265 + Pf1N1B4_3187 0.70 -0.0 3,570,266 - Pf1N1B4_3187 0.70 -0.3 3,570,282 + Pf1N1B4_3187 0.74 +0.9 3,570,282 + Pf1N1B4_3187 0.74 -0.0 3,570,282 + Pf1N1B4_3187 0.74 +0.5 3,570,282 + Pf1N1B4_3187 0.74 +0.1 3,570,282 + Pf1N1B4_3187 0.74 -0.0 3,570,282 + Pf1N1B4_3187 0.74 -1.6 3,570,282 + Pf1N1B4_3187 0.74 -1.3 3,570,283 - Pf1N1B4_3187 0.74 +0.1 3,570,283 - +1.0 3,570,283 - Pf1N1B4_3187 0.74 +0.2 3,570,283 - Pf1N1B4_3187 0.74 +1.8 3,570,283 - Pf1N1B4_3187 0.74 +1.5 3,570,283 - Pf1N1B4_3187 0.74 +0.0 3,570,283 - Pf1N1B4_3187 0.74 +0.4 3,570,283 - Pf1N1B4_3187 0.74 -0.6 3,570,283 - Pf1N1B4_3187 0.74 -0.3 3,570,286 + Pf1N1B4_3187 0.74 -0.8 3,570,286 + Pf1N1B4_3187 0.74 +0.5 3,570,287 - Pf1N1B4_3187 0.75 +0.5 3,570,287 - Pf1N1B4_3187 0.75 +0.5 3,570,287 - Pf1N1B4_3187 0.75 -0.2 3,570,287 - Pf1N1B4_3187 0.75 +0.1
Or see this region's nucleotide sequence