Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3174 and Pf1N1B4_3175 are separated by 92 nucleotides Pf1N1B4_3175 and Pf1N1B4_3176 are separated by 13 nucleotides Pf1N1B4_3176 and Pf1N1B4_3177 are separated by 12 nucleotides Pf1N1B4_3177 and Pf1N1B4_3178 overlap by 4 nucleotides
Pf1N1B4_3174 - Mobile element protein, at 3,556,095 to 3,557,123
_3174
Pf1N1B4_3175 - Histidine ABC transporter, ATP-binding protein HisP (TC 3.A.1.3.1), at 3,557,216 to 3,557,980
_3175
Pf1N1B4_3176 - Succinylglutamate desuccinylase/aspartoacylase, at 3,557,994 to 3,559,112
_3176
Pf1N1B4_3177 - Histidine ABC transporter, permease protein HisM (TC 3.A.1.3.1), at 3,559,125 to 3,559,835
_3177
Pf1N1B4_3178 - Histidine ABC transporter, permease protein HisQ (TC 3.A.1.3.1), at 3,559,832 to 3,560,560
_3178
Position (kb)
3557
3558
3559
3560 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3557.218 kb on - strand at 3557.218 kb on - strand at 3557.218 kb on - strand at 3557.273 kb on + strand at 3557.273 kb on + strand at 3557.273 kb on + strand at 3557.273 kb on + strand at 3557.273 kb on + strand at 3557.273 kb on + strand at 3557.274 kb on - strand at 3557.274 kb on - strand at 3557.274 kb on - strand at 3557.274 kb on - strand at 3557.274 kb on - strand at 3557.274 kb on - strand at 3557.586 kb on + strand, within Pf1N1B4_3175 at 3557.587 kb on - strand, within Pf1N1B4_3175 at 3557.943 kb on + strand at 3557.943 kb on + strand at 3557.944 kb on - strand at 3557.944 kb on - strand at 3557.950 kb on + strand at 3557.950 kb on + strand at 3557.951 kb on - strand at 3557.951 kb on - strand at 3557.976 kb on + strand at 3557.977 kb on - strand at 3557.977 kb on - strand at 3558.006 kb on + strand at 3558.006 kb on + strand at 3558.006 kb on + strand at 3558.006 kb on + strand at 3558.007 kb on - strand at 3558.007 kb on - strand at 3558.007 kb on - strand at 3558.007 kb on - strand at 3558.007 kb on - strand at 3558.007 kb on - strand at 3558.091 kb on + strand at 3558.091 kb on + strand at 3558.091 kb on + strand at 3558.092 kb on - strand at 3558.092 kb on - strand at 3558.092 kb on - strand at 3558.092 kb on - strand at 3558.092 kb on - strand at 3558.092 kb on - strand at 3558.181 kb on + strand, within Pf1N1B4_3176 at 3558.322 kb on + strand, within Pf1N1B4_3176 at 3558.323 kb on - strand, within Pf1N1B4_3176 at 3558.533 kb on - strand, within Pf1N1B4_3176 at 3558.577 kb on + strand, within Pf1N1B4_3176 at 3558.577 kb on + strand, within Pf1N1B4_3176 at 3558.577 kb on + strand, within Pf1N1B4_3176 at 3558.577 kb on + strand, within Pf1N1B4_3176 at 3558.578 kb on - strand, within Pf1N1B4_3176 at 3558.578 kb on - strand, within Pf1N1B4_3176 at 3558.735 kb on - strand, within Pf1N1B4_3176 at 3559.012 kb on + strand at 3559.012 kb on + strand at 3559.012 kb on + strand at 3559.012 kb on + strand at 3559.013 kb on - strand at 3559.013 kb on - strand at 3559.013 kb on - strand at 3559.114 kb on + strand at 3559.114 kb on + strand at 3559.114 kb on + strand at 3559.114 kb on + strand at 3559.114 kb on + strand at 3559.115 kb on - strand at 3559.115 kb on - strand at 3559.127 kb on - strand at 3559.207 kb on + strand, within Pf1N1B4_3177 at 3559.207 kb on + strand, within Pf1N1B4_3177 at 3559.208 kb on - strand, within Pf1N1B4_3177 at 3559.281 kb on + strand at 3559.281 kb on + strand, within Pf1N1B4_3177 at 3559.348 kb on + strand, within Pf1N1B4_3177 at 3559.348 kb on + strand, within Pf1N1B4_3177 at 3559.348 kb on + strand, within Pf1N1B4_3177 at 3559.348 kb on + strand, within Pf1N1B4_3177 at 3559.348 kb on + strand, within Pf1N1B4_3177 at 3559.349 kb on - strand, within Pf1N1B4_3177 at 3559.349 kb on - strand, within Pf1N1B4_3177 at 3559.351 kb on + strand, within Pf1N1B4_3177 at 3559.351 kb on + strand, within Pf1N1B4_3177 at 3559.351 kb on + strand, within Pf1N1B4_3177 at 3559.351 kb on + strand, within Pf1N1B4_3177 at 3559.352 kb on - strand, within Pf1N1B4_3177 at 3559.390 kb on + strand, within Pf1N1B4_3177 at 3559.390 kb on + strand, within Pf1N1B4_3177 at 3559.391 kb on - strand, within Pf1N1B4_3177 at 3559.391 kb on - strand, within Pf1N1B4_3177 at 3559.397 kb on - strand, within Pf1N1B4_3177 at 3559.397 kb on - strand, within Pf1N1B4_3177 at 3559.420 kb on + strand, within Pf1N1B4_3177 at 3559.489 kb on + strand, within Pf1N1B4_3177 at 3559.489 kb on + strand, within Pf1N1B4_3177 at 3559.588 kb on + strand, within Pf1N1B4_3177 at 3559.588 kb on + strand, within Pf1N1B4_3177 at 3559.588 kb on + strand, within Pf1N1B4_3177 at 3559.589 kb on - strand, within Pf1N1B4_3177 at 3559.589 kb on - strand, within Pf1N1B4_3177 at 3559.600 kb on + strand, within Pf1N1B4_3177 at 3559.601 kb on - strand, within Pf1N1B4_3177 at 3559.620 kb on - strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.622 kb on - strand, within Pf1N1B4_3177 at 3559.622 kb on - strand, within Pf1N1B4_3177 at 3559.622 kb on - strand at 3559.622 kb on - strand, within Pf1N1B4_3177 at 3559.622 kb on - strand, within Pf1N1B4_3177 at 3559.645 kb on + strand, within Pf1N1B4_3177 at 3559.645 kb on + strand, within Pf1N1B4_3177 at 3559.646 kb on - strand, within Pf1N1B4_3177 at 3559.646 kb on - strand, within Pf1N1B4_3177 at 3559.646 kb on - strand, within Pf1N1B4_3177 at 3559.646 kb on - strand, within Pf1N1B4_3177 at 3559.646 kb on - strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.652 kb on - strand, within Pf1N1B4_3177 at 3559.652 kb on - strand, within Pf1N1B4_3177 at 3559.652 kb on - strand, within Pf1N1B4_3177 at 3559.675 kb on + strand, within Pf1N1B4_3177 at 3559.675 kb on + strand, within Pf1N1B4_3177 at 3559.675 kb on + strand, within Pf1N1B4_3177 at 3559.675 kb on + strand, within Pf1N1B4_3177 at 3559.675 kb on + strand, within Pf1N1B4_3177 at 3559.676 kb on - strand, within Pf1N1B4_3177 at 3559.676 kb on - strand, within Pf1N1B4_3177 at 3559.676 kb on - strand at 3559.676 kb on - strand, within Pf1N1B4_3177 at 3559.783 kb on + strand at 3559.793 kb on + strand at 3559.794 kb on - strand at 3559.796 kb on - strand at 3559.796 kb on - strand at 3559.796 kb on - strand at 3559.813 kb on + strand at 3559.813 kb on + strand at 3559.945 kb on - strand, within Pf1N1B4_3178 at 3559.945 kb on - strand, within Pf1N1B4_3178 at 3559.956 kb on + strand, within Pf1N1B4_3178 at 3559.956 kb on + strand, within Pf1N1B4_3178 at 3559.956 kb on + strand, within Pf1N1B4_3178 at 3559.957 kb on - strand, within Pf1N1B4_3178 at 3559.957 kb on - strand, within Pf1N1B4_3178 at 3559.957 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 3,557,218 - +0.2 3,557,218 - -0.7 3,557,218 - -0.5 3,557,273 + -0.0 3,557,273 + -0.6 3,557,273 + -0.3 3,557,273 + +1.6 3,557,273 + -2.3 3,557,273 + +2.8 3,557,274 - -1.4 3,557,274 - +1.6 3,557,274 - -0.4 3,557,274 - +0.8 3,557,274 - +0.3 3,557,274 - -0.7 3,557,586 + Pf1N1B4_3175 0.48 -0.4 3,557,587 - Pf1N1B4_3175 0.48 -0.4 3,557,943 + -1.1 3,557,943 + +0.9 3,557,944 - +1.0 3,557,944 - -0.7 3,557,950 + -0.3 3,557,950 + +0.4 3,557,951 - -2.8 3,557,951 - +0.3 3,557,976 + -2.0 3,557,977 - -0.5 3,557,977 - -0.7 3,558,006 + -0.8 3,558,006 + -1.6 3,558,006 + -0.0 3,558,006 + +2.0 3,558,007 - +0.3 3,558,007 - -0.0 3,558,007 - +0.2 3,558,007 - +0.6 3,558,007 - -1.0 3,558,007 - +1.6 3,558,091 + -0.4 3,558,091 + -1.6 3,558,091 + -0.7 3,558,092 - +0.9 3,558,092 - -0.6 3,558,092 - +0.3 3,558,092 - -0.5 3,558,092 - -1.6 3,558,092 - -0.8 3,558,181 + Pf1N1B4_3176 0.17 +1.2 3,558,322 + Pf1N1B4_3176 0.29 -0.3 3,558,323 - Pf1N1B4_3176 0.29 -1.4 3,558,533 - Pf1N1B4_3176 0.48 -1.3 3,558,577 + Pf1N1B4_3176 0.52 +0.6 3,558,577 + Pf1N1B4_3176 0.52 +2.5 3,558,577 + Pf1N1B4_3176 0.52 +0.6 3,558,577 + Pf1N1B4_3176 0.52 -0.4 3,558,578 - Pf1N1B4_3176 0.52 +1.0 3,558,578 - Pf1N1B4_3176 0.52 +0.1 3,558,735 - Pf1N1B4_3176 0.66 -0.8 3,559,012 + -0.8 3,559,012 + -0.4 3,559,012 + -0.8 3,559,012 + -0.6 3,559,013 - +0.4 3,559,013 - -0.4 3,559,013 - -0.8 3,559,114 + +0.5 3,559,114 + +0.2 3,559,114 + +0.2 3,559,114 + +1.5 3,559,114 + +1.0 3,559,115 - -0.0 3,559,115 - -1.3 3,559,127 - +0.5 3,559,207 + Pf1N1B4_3177 0.12 -0.0 3,559,207 + Pf1N1B4_3177 0.12 +0.7 3,559,208 - Pf1N1B4_3177 0.12 -2.2 3,559,281 + +0.1 3,559,281 + Pf1N1B4_3177 0.22 +0.3 3,559,348 + Pf1N1B4_3177 0.31 -1.2 3,559,348 + Pf1N1B4_3177 0.31 +0.5 3,559,348 + Pf1N1B4_3177 0.31 +0.4 3,559,348 + Pf1N1B4_3177 0.31 -0.0 3,559,348 + Pf1N1B4_3177 0.31 -0.7 3,559,349 - Pf1N1B4_3177 0.32 +0.7 3,559,349 - Pf1N1B4_3177 0.32 -2.3 3,559,351 + Pf1N1B4_3177 0.32 -0.0 3,559,351 + Pf1N1B4_3177 0.32 +0.2 3,559,351 + Pf1N1B4_3177 0.32 -1.3 3,559,351 + Pf1N1B4_3177 0.32 -0.8 3,559,352 - Pf1N1B4_3177 0.32 -0.7 3,559,390 + Pf1N1B4_3177 0.37 -0.0 3,559,390 + Pf1N1B4_3177 0.37 +0.5 3,559,391 - Pf1N1B4_3177 0.37 -0.3 3,559,391 - Pf1N1B4_3177 0.37 -1.6 3,559,397 - Pf1N1B4_3177 0.38 -0.5 3,559,397 - Pf1N1B4_3177 0.38 +0.2 3,559,420 + Pf1N1B4_3177 0.41 +1.3 3,559,489 + Pf1N1B4_3177 0.51 -1.0 3,559,489 + Pf1N1B4_3177 0.51 +0.5 3,559,588 + Pf1N1B4_3177 0.65 -1.5 3,559,588 + Pf1N1B4_3177 0.65 -0.0 3,559,588 + Pf1N1B4_3177 0.65 -0.6 3,559,589 - Pf1N1B4_3177 0.65 +2.1 3,559,589 - Pf1N1B4_3177 0.65 -1.6 3,559,600 + Pf1N1B4_3177 0.67 -1.3 3,559,601 - Pf1N1B4_3177 0.67 -1.0 3,559,620 - Pf1N1B4_3177 0.70 -0.9 3,559,621 + Pf1N1B4_3177 0.70 -0.9 3,559,621 + Pf1N1B4_3177 0.70 -0.3 3,559,621 + Pf1N1B4_3177 0.70 +2.1 3,559,621 + Pf1N1B4_3177 0.70 +1.0 3,559,621 + Pf1N1B4_3177 0.70 +0.5 3,559,621 + Pf1N1B4_3177 0.70 +1.1 3,559,621 + Pf1N1B4_3177 0.70 +0.3 3,559,621 + Pf1N1B4_3177 0.70 -0.8 3,559,621 + Pf1N1B4_3177 0.70 -0.2 3,559,622 - Pf1N1B4_3177 0.70 -1.6 3,559,622 - Pf1N1B4_3177 0.70 +0.5 3,559,622 - -0.3 3,559,622 - Pf1N1B4_3177 0.70 +0.7 3,559,622 - Pf1N1B4_3177 0.70 -0.8 3,559,645 + Pf1N1B4_3177 0.73 +0.5 3,559,645 + Pf1N1B4_3177 0.73 +0.5 3,559,646 - Pf1N1B4_3177 0.73 -1.6 3,559,646 - Pf1N1B4_3177 0.73 +1.1 3,559,646 - Pf1N1B4_3177 0.73 +1.0 3,559,646 - Pf1N1B4_3177 0.73 +1.5 3,559,646 - Pf1N1B4_3177 0.73 +0.5 3,559,651 + Pf1N1B4_3177 0.74 -1.1 3,559,651 + Pf1N1B4_3177 0.74 +3.0 3,559,651 + Pf1N1B4_3177 0.74 +2.0 3,559,651 + Pf1N1B4_3177 0.74 -0.0 3,559,651 + Pf1N1B4_3177 0.74 -0.6 3,559,651 + Pf1N1B4_3177 0.74 +0.5 3,559,652 - Pf1N1B4_3177 0.74 -0.5 3,559,652 - Pf1N1B4_3177 0.74 -0.9 3,559,652 - Pf1N1B4_3177 0.74 -0.0 3,559,675 + Pf1N1B4_3177 0.77 +0.5 3,559,675 + Pf1N1B4_3177 0.77 +0.9 3,559,675 + Pf1N1B4_3177 0.77 +2.0 3,559,675 + Pf1N1B4_3177 0.77 -0.2 3,559,675 + Pf1N1B4_3177 0.77 +0.8 3,559,676 - Pf1N1B4_3177 0.77 -0.2 3,559,676 - Pf1N1B4_3177 0.77 +1.5 3,559,676 - -0.0 3,559,676 - Pf1N1B4_3177 0.77 -1.3 3,559,783 + -0.5 3,559,793 + -0.8 3,559,794 - +0.5 3,559,796 - +1.0 3,559,796 - +0.9 3,559,796 - -1.0 3,559,813 + +0.3 3,559,813 + -0.7 3,559,945 - Pf1N1B4_3178 0.16 +2.6 3,559,945 - Pf1N1B4_3178 0.16 +1.0 3,559,956 + Pf1N1B4_3178 0.17 +2.6 3,559,956 + Pf1N1B4_3178 0.17 -0.9 3,559,956 + Pf1N1B4_3178 0.17 -2.1 3,559,957 - Pf1N1B4_3178 0.17 -1.3 3,559,957 - Pf1N1B4_3178 0.17 -0.6 3,559,957 - -0.8
Or see this region's nucleotide sequence