Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3151 and Pf1N1B4_3152 overlap by 4 nucleotides Pf1N1B4_3152 and Pf1N1B4_3153 are separated by 37 nucleotides Pf1N1B4_3153 and Pf1N1B4_3154 overlap by 39 nucleotides Pf1N1B4_3154 and Pf1N1B4_3155 are separated by 98 nucleotides
Pf1N1B4_3151 - Diacylglycerol kinase (EC 2.7.1.107), at 3,531,448 to 3,531,810
_3151
Pf1N1B4_3152 - LysR family transcriptional regulator PA3398, at 3,531,807 to 3,532,733
_3152
Pf1N1B4_3153 - hypothetical protein, at 3,532,771 to 3,532,893
_3153
Pf1N1B4_3154 - Ferredoxin--NADP(+) reductase (EC 1.18.1.2), at 3,532,855 to 3,533,634
_3154
Pf1N1B4_3155 - COG1720: Uncharacterized conserved protein, at 3,533,733 to 3,534,437
_3155
Position (kb)
3532
3533 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3531.808 kb on + strand at 3531.808 kb on + strand at 3531.905 kb on - strand at 3531.905 kb on - strand at 3531.928 kb on + strand, within Pf1N1B4_3152 at 3531.928 kb on + strand, within Pf1N1B4_3152 at 3531.928 kb on + strand, within Pf1N1B4_3152 at 3531.928 kb on + strand, within Pf1N1B4_3152 at 3531.928 kb on + strand, within Pf1N1B4_3152 at 3531.929 kb on - strand, within Pf1N1B4_3152 at 3531.929 kb on - strand, within Pf1N1B4_3152 at 3532.300 kb on - strand, within Pf1N1B4_3152 at 3532.319 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.424 kb on - strand, within Pf1N1B4_3152 at 3532.431 kb on + strand, within Pf1N1B4_3152 at 3532.704 kb on + strand at 3532.724 kb on + strand at 3532.724 kb on + strand at 3532.724 kb on + strand at 3532.779 kb on + strand at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3532.794 kb on + strand at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3532.794 kb on + strand at 3532.794 kb on + strand, within Pf1N1B4_3153 at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.632 kb on + strand at 3533.633 kb on - strand at 3533.633 kb on - strand at 3533.633 kb on - strand at 3533.633 kb on - strand at 3533.633 kb on - strand at 3533.734 kb on + strand at 3533.735 kb on - strand at 3533.735 kb on - strand at 3533.802 kb on + strand at 3533.802 kb on + strand at 3533.802 kb on + strand at 3533.802 kb on + strand at 3533.802 kb on + strand at 3533.802 kb on + strand at 3533.803 kb on - strand at 3533.803 kb on - strand at 3533.803 kb on - strand at 3533.803 kb on - strand at 3533.803 kb on - strand at 3533.831 kb on - strand, within Pf1N1B4_3155 at 3533.847 kb on - strand, within Pf1N1B4_3155 at 3533.847 kb on - strand, within Pf1N1B4_3155
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 3,531,808 + -0.4 3,531,808 + -1.2 3,531,905 - +0.6 3,531,905 - -0.0 3,531,928 + Pf1N1B4_3152 0.13 +1.6 3,531,928 + Pf1N1B4_3152 0.13 +1.3 3,531,928 + Pf1N1B4_3152 0.13 +1.3 3,531,928 + Pf1N1B4_3152 0.13 +0.5 3,531,928 + Pf1N1B4_3152 0.13 -0.7 3,531,929 - Pf1N1B4_3152 0.13 +0.4 3,531,929 - Pf1N1B4_3152 0.13 -1.8 3,532,300 - Pf1N1B4_3152 0.53 -1.4 3,532,319 - Pf1N1B4_3152 0.55 -1.9 3,532,424 - Pf1N1B4_3152 0.67 -0.7 3,532,424 - Pf1N1B4_3152 0.67 -0.9 3,532,424 - Pf1N1B4_3152 0.67 -2.2 3,532,424 - Pf1N1B4_3152 0.67 -1.4 3,532,424 - Pf1N1B4_3152 0.67 -0.0 3,532,424 - Pf1N1B4_3152 0.67 +0.2 3,532,424 - Pf1N1B4_3152 0.67 -1.6 3,532,424 - Pf1N1B4_3152 0.67 -0.0 3,532,424 - Pf1N1B4_3152 0.67 -0.2 3,532,424 - Pf1N1B4_3152 0.67 -2.2 3,532,431 + Pf1N1B4_3152 0.67 +0.6 3,532,704 + -1.0 3,532,724 + -0.5 3,532,724 + +0.8 3,532,724 + -1.8 3,532,779 + +0.6 3,532,794 + Pf1N1B4_3153 0.19 +0.6 3,532,794 + Pf1N1B4_3153 0.19 +1.6 3,532,794 + Pf1N1B4_3153 0.19 +1.8 3,532,794 + +2.1 3,532,794 + Pf1N1B4_3153 0.19 +1.1 3,532,794 + Pf1N1B4_3153 0.19 +0.1 3,532,794 + -0.0 3,532,794 + Pf1N1B4_3153 0.19 -0.2 3,533,632 + -0.4 3,533,632 + -1.4 3,533,632 + +0.9 3,533,632 + -2.5 3,533,632 + +1.0 3,533,632 + -2.0 3,533,632 + -1.2 3,533,632 + -0.4 3,533,633 - +1.6 3,533,633 - +0.6 3,533,633 - -0.2 3,533,633 - +0.3 3,533,633 - +0.6 3,533,734 + -0.8 3,533,735 - +0.6 3,533,735 - -2.1 3,533,802 + -1.2 3,533,802 + +0.2 3,533,802 + -0.8 3,533,802 + +0.2 3,533,802 + -0.0 3,533,802 + -0.4 3,533,803 - -0.1 3,533,803 - +0.6 3,533,803 - -0.0 3,533,803 - -1.0 3,533,803 - -1.0 3,533,831 - Pf1N1B4_3155 0.14 -2.1 3,533,847 - Pf1N1B4_3155 0.16 -0.6 3,533,847 - Pf1N1B4_3155 0.16 +1.6
Or see this region's nucleotide sequence