Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3114 and Pf1N1B4_3115 are separated by 548 nucleotides Pf1N1B4_3115 and Pf1N1B4_3116 are separated by 485 nucleotides Pf1N1B4_3116 and Pf1N1B4_3117 are separated by 142 nucleotides
Pf1N1B4_3114 - hypothetical protein, at 3,500,678 to 3,500,806
_3114
Pf1N1B4_3115 - RNA polymerase sigma factor RpoS, at 3,501,355 to 3,502,362
_3115
Pf1N1B4_3116 - 4Fe-4S ferredoxin, iron-sulfur binding, at 3,502,848 to 3,503,171
_3116
Pf1N1B4_3117 - DNA mismatch repair protein MutS, at 3,503,314 to 3,505,893
_3117
Position (kb)
3501
3502
3503 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3501.276 kb on - strand at 3501.348 kb on + strand at 3501.350 kb on + strand at 3501.350 kb on + strand at 3501.351 kb on - strand at 3501.367 kb on - strand at 3501.502 kb on - strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.514 kb on + strand, within Pf1N1B4_3115 at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.515 kb on - strand at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.515 kb on - strand at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.515 kb on - strand, within Pf1N1B4_3115 at 3501.523 kb on + strand, within Pf1N1B4_3115 at 3501.554 kb on - strand, within Pf1N1B4_3115 at 3501.554 kb on - strand at 3501.554 kb on - strand, within Pf1N1B4_3115 at 3501.554 kb on - strand, within Pf1N1B4_3115 at 3501.697 kb on + strand, within Pf1N1B4_3115 at 3501.764 kb on + strand, within Pf1N1B4_3115 at 3501.764 kb on + strand, within Pf1N1B4_3115 at 3501.764 kb on + strand at 3501.805 kb on + strand, within Pf1N1B4_3115 at 3501.806 kb on - strand, within Pf1N1B4_3115 at 3501.806 kb on - strand, within Pf1N1B4_3115 at 3501.864 kb on + strand, within Pf1N1B4_3115 at 3501.904 kb on + strand, within Pf1N1B4_3115 at 3501.904 kb on + strand, within Pf1N1B4_3115 at 3501.904 kb on + strand at 3501.904 kb on + strand at 3501.905 kb on - strand, within Pf1N1B4_3115 at 3501.905 kb on - strand at 3502.010 kb on + strand, within Pf1N1B4_3115 at 3502.010 kb on + strand, within Pf1N1B4_3115 at 3502.011 kb on - strand, within Pf1N1B4_3115 at 3502.066 kb on - strand, within Pf1N1B4_3115 at 3502.066 kb on - strand at 3502.244 kb on + strand, within Pf1N1B4_3115 at 3502.244 kb on + strand, within Pf1N1B4_3115 at 3502.244 kb on + strand, within Pf1N1B4_3115 at 3502.244 kb on + strand, within Pf1N1B4_3115 at 3502.245 kb on - strand, within Pf1N1B4_3115 at 3502.423 kb on + strand at 3502.423 kb on + strand at 3502.423 kb on + strand at 3502.424 kb on - strand at 3502.424 kb on - strand at 3502.424 kb on - strand at 3502.425 kb on + strand at 3502.425 kb on + strand at 3502.425 kb on + strand at 3502.425 kb on + strand at 3502.425 kb on + strand at 3502.426 kb on - strand at 3502.426 kb on - strand at 3502.426 kb on - strand at 3502.463 kb on + strand at 3502.463 kb on + strand at 3502.476 kb on + strand at 3502.476 kb on + strand at 3502.476 kb on + strand at 3502.477 kb on - strand at 3502.489 kb on - strand at 3502.499 kb on - strand at 3502.516 kb on - strand at 3502.537 kb on + strand at 3502.636 kb on - strand at 3502.650 kb on + strand at 3502.651 kb on - strand at 3502.653 kb on + strand at 3502.654 kb on - strand at 3503.241 kb on - strand at 3503.292 kb on + strand at 3503.293 kb on - strand at 3503.315 kb on + strand at 3503.315 kb on + strand at 3503.315 kb on + strand at 3503.315 kb on + strand at 3503.315 kb on + strand at 3503.316 kb on - strand at 3503.329 kb on + strand at 3503.329 kb on + strand at 3503.330 kb on - strand at 3503.330 kb on - strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.334 kb on + strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.335 kb on - strand at 3503.336 kb on + strand at 3503.336 kb on + strand at 3503.336 kb on + strand at 3503.336 kb on + strand at 3503.336 kb on + strand at 3503.336 kb on + strand at 3503.337 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 3,501,276 - +3.4 3,501,348 + +3.8 3,501,350 + +3.6 3,501,350 + +3.5 3,501,351 - +3.8 3,501,367 - +4.7 3,501,502 - Pf1N1B4_3115 0.15 +3.9 3,501,514 + Pf1N1B4_3115 0.16 +3.6 3,501,514 + Pf1N1B4_3115 0.16 +3.6 3,501,514 + Pf1N1B4_3115 0.16 +3.6 3,501,514 + Pf1N1B4_3115 0.16 +3.3 3,501,514 + Pf1N1B4_3115 0.16 +3.9 3,501,514 + Pf1N1B4_3115 0.16 +4.4 3,501,514 + Pf1N1B4_3115 0.16 +4.2 3,501,514 + Pf1N1B4_3115 0.16 +3.9 3,501,515 - Pf1N1B4_3115 0.16 +4.7 3,501,515 - +3.0 3,501,515 - Pf1N1B4_3115 0.16 +4.1 3,501,515 - +4.6 3,501,515 - Pf1N1B4_3115 0.16 +3.4 3,501,515 - Pf1N1B4_3115 0.16 +4.5 3,501,515 - Pf1N1B4_3115 0.16 +3.8 3,501,515 - Pf1N1B4_3115 0.16 +3.6 3,501,523 + Pf1N1B4_3115 0.17 +3.6 3,501,554 - Pf1N1B4_3115 0.20 +3.5 3,501,554 - +4.1 3,501,554 - Pf1N1B4_3115 0.20 +3.3 3,501,554 - Pf1N1B4_3115 0.20 +3.3 3,501,697 + Pf1N1B4_3115 0.34 +4.3 3,501,764 + Pf1N1B4_3115 0.41 +4.1 3,501,764 + Pf1N1B4_3115 0.41 +4.1 3,501,764 + +3.9 3,501,805 + Pf1N1B4_3115 0.45 +3.2 3,501,806 - Pf1N1B4_3115 0.45 +3.6 3,501,806 - Pf1N1B4_3115 0.45 +3.2 3,501,864 + Pf1N1B4_3115 0.50 +3.8 3,501,904 + Pf1N1B4_3115 0.54 +4.1 3,501,904 + Pf1N1B4_3115 0.54 +5.5 3,501,904 + +4.7 3,501,904 + +2.5 3,501,905 - Pf1N1B4_3115 0.55 +3.5 3,501,905 - +3.4 3,502,010 + Pf1N1B4_3115 0.65 +3.6 3,502,010 + Pf1N1B4_3115 0.65 +3.8 3,502,011 - Pf1N1B4_3115 0.65 +4.0 3,502,066 - Pf1N1B4_3115 0.71 +4.3 3,502,066 - +3.9 3,502,244 + Pf1N1B4_3115 0.88 +3.4 3,502,244 + Pf1N1B4_3115 0.88 +3.3 3,502,244 + Pf1N1B4_3115 0.88 +1.7 3,502,244 + Pf1N1B4_3115 0.88 +3.1 3,502,245 - Pf1N1B4_3115 0.88 +3.8 3,502,423 + +0.6 3,502,423 + +2.9 3,502,423 + -0.8 3,502,424 - +0.1 3,502,424 - +1.5 3,502,424 - +0.3 3,502,425 + -1.3 3,502,425 + -0.9 3,502,425 + +1.1 3,502,425 + +0.9 3,502,425 + -1.5 3,502,426 - +1.8 3,502,426 - +1.5 3,502,426 - -0.0 3,502,463 + +0.5 3,502,463 + +1.5 3,502,476 + +0.3 3,502,476 + -1.5 3,502,476 + +1.5 3,502,477 - +1.0 3,502,489 - +0.3 3,502,499 - +0.5 3,502,516 - +0.9 3,502,537 + -0.6 3,502,636 - +0.3 3,502,650 + +2.0 3,502,651 - -2.3 3,502,653 + +1.5 3,502,654 - -0.2 3,503,241 - -1.6 3,503,292 + -1.9 3,503,293 - -2.3 3,503,315 + +0.3 3,503,315 + -1.0 3,503,315 + +0.4 3,503,315 + -0.3 3,503,315 + -0.8 3,503,316 - +0.8 3,503,329 + -0.9 3,503,329 + -1.6 3,503,330 - +0.8 3,503,330 - -0.9 3,503,334 + -1.3 3,503,334 + -2.4 3,503,334 + +0.2 3,503,334 + +0.7 3,503,334 + +0.4 3,503,334 + +1.9 3,503,334 + -0.6 3,503,334 + +2.1 3,503,334 + +0.0 3,503,334 + +1.5 3,503,334 + +0.4 3,503,334 + -0.6 3,503,334 + +0.9 3,503,334 + -1.2 3,503,334 + +0.7 3,503,334 + -1.8 3,503,334 + +1.0 3,503,334 + -0.5 3,503,334 + +0.2 3,503,334 + +1.0 3,503,334 + +0.9 3,503,334 + -0.8 3,503,334 + -0.8 3,503,335 - +1.4 3,503,335 - -1.0 3,503,335 - -2.1 3,503,335 - +2.1 3,503,335 - +0.7 3,503,335 - +0.3 3,503,335 - -0.0 3,503,335 - +0.6 3,503,335 - -0.5 3,503,335 - +0.1 3,503,335 - +3.0 3,503,336 + +1.2 3,503,336 + -0.4 3,503,336 + +1.4 3,503,336 + -0.6 3,503,336 + -0.0 3,503,336 + -0.0 3,503,337 - -0.8
Or see this region's nucleotide sequence