Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_308 and Pf1N1B4_309 are separated by 44 nucleotides Pf1N1B4_309 and Pf1N1B4_310 are separated by 245 nucleotides
Pf1N1B4_308 - FIG00956090: hypothetical protein, at 361,880 to 362,647
_308
Pf1N1B4_309 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 362,692 to 362,970
_309
Pf1N1B4_310 - UPF0028 protein YchK, at 363,216 to 364,256
_310
Position (kb) 362
363 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 361.699 kb on + strand at 361.700 kb on - strand at 362.115 kb on + strand, within Pf1N1B4_308 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.534 kb on + strand, within Pf1N1B4_308 at 362.534 kb on + strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.659 kb on + strand at 362.659 kb on + strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.720 kb on + strand, within Pf1N1B4_309 at 362.721 kb on - strand, within Pf1N1B4_309 at 362.721 kb on - strand, within Pf1N1B4_309 at 362.721 kb on - strand, within Pf1N1B4_309 at 362.759 kb on + strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.817 kb on - strand, within Pf1N1B4_309 at 362.988 kb on + strand at 362.988 kb on + strand at 362.988 kb on + strand at 362.988 kb on + strand at 362.992 kb on + strand at 362.992 kb on + strand at 362.992 kb on + strand at 362.992 kb on + strand at 362.993 kb on - strand at 362.993 kb on - strand at 362.993 kb on - strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.082 kb on + strand at 363.082 kb on + strand at 363.082 kb on + strand at 363.082 kb on + strand at 363.083 kb on - strand at 363.083 kb on - strand at 363.083 kb on - strand at 363.083 kb on - strand at 363.360 kb on + strand, within Pf1N1B4_310 at 363.360 kb on + strand, within Pf1N1B4_310 at 363.360 kb on + strand, within Pf1N1B4_310 at 363.360 kb on + strand, within Pf1N1B4_310 at 363.361 kb on - strand, within Pf1N1B4_310 at 363.361 kb on - strand, within Pf1N1B4_310 at 363.361 kb on - strand at 363.411 kb on + strand, within Pf1N1B4_310 at 363.509 kb on + strand, within Pf1N1B4_310 at 363.509 kb on + strand, within Pf1N1B4_310 at 363.510 kb on - strand, within Pf1N1B4_310 at 363.510 kb on - strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310 at 363.771 kb on + strand, within Pf1N1B4_310 at 363.771 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 361,699 + -1.8 361,700 - -0.4 362,115 + Pf1N1B4_308 0.31 -0.5 362,398 - Pf1N1B4_308 0.67 -1.9 362,398 - Pf1N1B4_308 0.67 +0.5 362,398 - Pf1N1B4_308 0.67 -2.1 362,534 + Pf1N1B4_308 0.85 -0.8 362,534 + Pf1N1B4_308 0.85 +0.8 362,535 - Pf1N1B4_308 0.85 +0.2 362,535 - Pf1N1B4_308 0.85 -0.8 362,535 - Pf1N1B4_308 0.85 -1.2 362,535 - Pf1N1B4_308 0.85 -1.6 362,535 - Pf1N1B4_308 0.85 -1.4 362,535 - Pf1N1B4_308 0.85 +0.2 362,659 + +1.0 362,659 + +0.6 362,684 - -0.8 362,684 - -0.1 362,684 - -1.8 362,684 - -1.2 362,684 - -2.8 362,720 + Pf1N1B4_309 0.10 -1.6 362,721 - Pf1N1B4_309 0.10 -0.8 362,721 - Pf1N1B4_309 0.10 -1.3 362,721 - Pf1N1B4_309 0.10 -2.8 362,759 + Pf1N1B4_309 0.24 +1.9 362,760 - Pf1N1B4_309 0.24 +0.3 362,760 - Pf1N1B4_309 0.24 -2.0 362,760 - Pf1N1B4_309 0.24 -0.5 362,760 - Pf1N1B4_309 0.24 -0.9 362,817 - Pf1N1B4_309 0.45 -1.2 362,988 + +0.6 362,988 + -0.0 362,988 + -0.4 362,988 + -0.8 362,992 + +2.1 362,992 + +1.3 362,992 + +0.3 362,992 + -1.0 362,993 - +0.8 362,993 - -0.7 362,993 - +1.2 362,996 + -1.8 362,996 + -1.3 362,996 + +0.3 362,996 + -0.0 362,996 + -2.3 362,996 + +1.3 362,996 + +0.3 362,996 + -0.0 362,996 + +1.0 362,996 + +1.0 362,996 + -0.2 362,996 + -1.2 362,997 - -1.2 362,997 - +0.2 362,997 - -1.0 362,997 - +1.2 362,997 - +2.2 362,997 - +1.1 362,997 - -0.8 362,997 - -0.3 362,997 - -0.1 363,008 + +2.6 363,008 + -0.5 363,008 + +1.2 363,008 + +0.3 363,008 + +2.2 363,009 - -1.5 363,009 - -0.6 363,009 - +0.3 363,009 - -0.9 363,009 - -1.1 363,082 + +0.8 363,082 + -1.1 363,082 + +1.2 363,082 + +0.6 363,083 - +1.6 363,083 - +1.0 363,083 - +1.3 363,083 - -0.8 363,360 + Pf1N1B4_310 0.14 -0.2 363,360 + Pf1N1B4_310 0.14 +1.0 363,360 + Pf1N1B4_310 0.14 +0.6 363,360 + Pf1N1B4_310 0.14 +2.6 363,361 - Pf1N1B4_310 0.14 +0.3 363,361 - Pf1N1B4_310 0.14 +0.1 363,361 - +1.2 363,411 + Pf1N1B4_310 0.19 +1.1 363,509 + Pf1N1B4_310 0.28 -1.2 363,509 + Pf1N1B4_310 0.28 -0.5 363,510 - Pf1N1B4_310 0.28 +0.8 363,510 - Pf1N1B4_310 0.28 -0.8 363,512 + Pf1N1B4_310 0.28 +0.1 363,512 + Pf1N1B4_310 0.28 +1.4 363,512 + Pf1N1B4_310 0.28 -0.1 363,771 + Pf1N1B4_310 0.53 +1.6 363,771 + -0.2
Or see this region's nucleotide sequence