Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2748

Experiment: Nitrate reduction, replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2747 and Pf1N1B4_2748 are separated by 103 nucleotidesPf1N1B4_2748 and Pf1N1B4_2749 are separated by 75 nucleotidesPf1N1B4_2749 and Pf1N1B4_2750 are separated by 5 nucleotides Pf1N1B4_2747 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 3,086,298 to 3,088,199 _2747 Pf1N1B4_2748 - Transcriptional regulator, TetR family, at 3,088,303 to 3,088,980 _2748 Pf1N1B4_2749 - Quinate/shikimate 5-dehydrogenase I delta (EC 1.1.1.25), at 3,089,056 to 3,089,910 _2749 Pf1N1B4_2750 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 3,089,916 to 3,090,356 _2750 Position (kb) 3088 3089Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3087.343 kb on - strand, within Pf1N1B4_2747at 3087.444 kb on + strand, within Pf1N1B4_2747at 3087.444 kb on + strand, within Pf1N1B4_2747at 3087.444 kb on + strand, within Pf1N1B4_2747at 3087.445 kb on - strand, within Pf1N1B4_2747at 3087.528 kb on + strand, within Pf1N1B4_2747at 3087.555 kb on + strand, within Pf1N1B4_2747at 3087.556 kb on - strand, within Pf1N1B4_2747at 3087.556 kb on - strand, within Pf1N1B4_2747at 3087.669 kb on + strand, within Pf1N1B4_2747at 3087.669 kb on + strand, within Pf1N1B4_2747at 3087.670 kb on - strandat 3087.670 kb on - strand, within Pf1N1B4_2747at 3087.670 kb on - strand, within Pf1N1B4_2747at 3087.671 kb on + strand, within Pf1N1B4_2747at 3087.672 kb on - strand, within Pf1N1B4_2747at 3087.672 kb on - strand, within Pf1N1B4_2747at 3087.672 kb on - strandat 3087.724 kb on - strandat 3087.939 kb on + strand, within Pf1N1B4_2747at 3087.940 kb on - strandat 3087.940 kb on - strand, within Pf1N1B4_2747at 3087.940 kb on - strand, within Pf1N1B4_2747at 3087.940 kb on - strand, within Pf1N1B4_2747at 3087.940 kb on - strand, within Pf1N1B4_2747at 3087.942 kb on + strand, within Pf1N1B4_2747at 3087.942 kb on + strand, within Pf1N1B4_2747at 3087.943 kb on - strand, within Pf1N1B4_2747at 3087.943 kb on - strand, within Pf1N1B4_2747at 3087.943 kb on - strand, within Pf1N1B4_2747at 3088.014 kb on + strandat 3088.053 kb on + strandat 3088.053 kb on + strandat 3088.053 kb on + strandat 3088.053 kb on + strandat 3088.054 kb on - strandat 3088.054 kb on - strandat 3088.054 kb on - strandat 3088.181 kb on - strandat 3088.181 kb on - strandat 3088.197 kb on + strandat 3088.208 kb on + strandat 3088.222 kb on + strandat 3088.222 kb on + strandat 3088.222 kb on + strandat 3088.222 kb on + strandat 3088.222 kb on + strandat 3088.222 kb on + strandat 3088.222 kb on + strandat 3088.222 kb on + strandat 3088.222 kb on + strandat 3088.222 kb on + strandat 3088.222 kb on + strandat 3088.223 kb on - strandat 3088.223 kb on - strandat 3088.223 kb on - strandat 3088.223 kb on - strandat 3088.223 kb on - strandat 3088.223 kb on - strandat 3088.223 kb on - strandat 3088.223 kb on - strandat 3088.223 kb on - strandat 3088.310 kb on + strandat 3088.310 kb on + strandat 3088.310 kb on + strandat 3088.311 kb on - strandat 3088.311 kb on - strandat 3088.311 kb on - strandat 3088.311 kb on - strandat 3088.314 kb on + strandat 3088.314 kb on + strandat 3088.314 kb on + strandat 3088.315 kb on - strandat 3088.315 kb on - strandat 3088.315 kb on - strandat 3088.315 kb on - strandat 3088.315 kb on - strandat 3088.498 kb on + strand, within Pf1N1B4_2748at 3088.498 kb on + strand, within Pf1N1B4_2748at 3088.498 kb on + strand, within Pf1N1B4_2748at 3088.498 kb on + strand, within Pf1N1B4_2748at 3088.498 kb on + strand, within Pf1N1B4_2748at 3088.498 kb on + strand, within Pf1N1B4_2748at 3088.498 kb on + strand, within Pf1N1B4_2748at 3088.498 kb on + strand, within Pf1N1B4_2748at 3088.498 kb on + strand, within Pf1N1B4_2748at 3088.498 kb on + strand, within Pf1N1B4_2748at 3088.499 kb on - strand, within Pf1N1B4_2748at 3088.499 kb on - strand, within Pf1N1B4_2748at 3088.499 kb on - strand, within Pf1N1B4_2748at 3088.499 kb on - strand, within Pf1N1B4_2748at 3088.499 kb on - strand, within Pf1N1B4_2748at 3088.499 kb on - strand, within Pf1N1B4_2748at 3088.499 kb on - strand, within Pf1N1B4_2748at 3088.499 kb on - strand, within Pf1N1B4_2748at 3088.501 kb on + strand, within Pf1N1B4_2748at 3088.502 kb on - strand, within Pf1N1B4_2748at 3088.502 kb on - strand, within Pf1N1B4_2748at 3088.504 kb on + strand, within Pf1N1B4_2748at 3088.504 kb on + strand, within Pf1N1B4_2748at 3088.504 kb on + strand, within Pf1N1B4_2748at 3088.505 kb on - strand, within Pf1N1B4_2748at 3088.505 kb on - strand, within Pf1N1B4_2748at 3088.505 kb on - strand, within Pf1N1B4_2748at 3088.505 kb on - strand, within Pf1N1B4_2748at 3088.515 kb on + strand, within Pf1N1B4_2748at 3088.515 kb on + strand, within Pf1N1B4_2748at 3088.528 kb on + strand, within Pf1N1B4_2748at 3088.528 kb on + strand, within Pf1N1B4_2748at 3088.553 kb on - strand, within Pf1N1B4_2748at 3088.708 kb on + strand, within Pf1N1B4_2748at 3088.799 kb on + strand, within Pf1N1B4_2748at 3088.799 kb on + strand, within Pf1N1B4_2748at 3088.799 kb on + strand, within Pf1N1B4_2748at 3088.799 kb on + strand, within Pf1N1B4_2748at 3088.799 kb on + strand, within Pf1N1B4_2748at 3088.799 kb on + strand, within Pf1N1B4_2748at 3088.800 kb on - strand, within Pf1N1B4_2748at 3088.800 kb on - strand, within Pf1N1B4_2748at 3088.800 kb on - strand, within Pf1N1B4_2748at 3088.859 kb on - strand, within Pf1N1B4_2748at 3088.878 kb on + strand, within Pf1N1B4_2748at 3088.878 kb on + strand, within Pf1N1B4_2748at 3088.879 kb on - strand, within Pf1N1B4_2748at 3088.879 kb on - strand, within Pf1N1B4_2748at 3089.007 kb on - strandat 3089.226 kb on - strand, within Pf1N1B4_2749at 3089.226 kb on - strand, within Pf1N1B4_2749at 3089.226 kb on - strand, within Pf1N1B4_2749at 3089.226 kb on - strand, within Pf1N1B4_2749at 3089.314 kb on - strand, within Pf1N1B4_2749at 3089.455 kb on + strand, within Pf1N1B4_2749at 3089.455 kb on + strand, within Pf1N1B4_2749at 3089.456 kb on - strand, within Pf1N1B4_2749at 3089.456 kb on - strand, within Pf1N1B4_2749at 3089.456 kb on - strand, within Pf1N1B4_2749at 3089.456 kb on - strand, within Pf1N1B4_2749at 3089.471 kb on - strand, within Pf1N1B4_2749at 3089.515 kb on + strand, within Pf1N1B4_2749at 3089.516 kb on - strand, within Pf1N1B4_2749at 3089.516 kb on - strand, within Pf1N1B4_2749at 3089.805 kb on - strand, within Pf1N1B4_2749

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C
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3,087,343 - Pf1N1B4_2747 0.55 +0.2
3,087,444 + Pf1N1B4_2747 0.60 -0.6
3,087,444 + Pf1N1B4_2747 0.60 -0.6
3,087,444 + Pf1N1B4_2747 0.60 -2.2
3,087,445 - Pf1N1B4_2747 0.60 +0.2
3,087,528 + Pf1N1B4_2747 0.65 -0.9
3,087,555 + Pf1N1B4_2747 0.66 +0.9
3,087,556 - Pf1N1B4_2747 0.66 +1.6
3,087,556 - Pf1N1B4_2747 0.66 +1.2
3,087,669 + Pf1N1B4_2747 0.72 -0.6
3,087,669 + Pf1N1B4_2747 0.72 +1.8
3,087,670 - -1.0
3,087,670 - Pf1N1B4_2747 0.72 -1.6
3,087,670 - Pf1N1B4_2747 0.72 -0.8
3,087,671 + Pf1N1B4_2747 0.72 -2.5
3,087,672 - Pf1N1B4_2747 0.72 -1.0
3,087,672 - Pf1N1B4_2747 0.72 +2.2
3,087,672 - +0.6
3,087,724 - +3.1
3,087,939 + Pf1N1B4_2747 0.86 -0.4
3,087,940 - +0.6
3,087,940 - Pf1N1B4_2747 0.86 +0.6
3,087,940 - Pf1N1B4_2747 0.86 +0.3
3,087,940 - Pf1N1B4_2747 0.86 -0.8
3,087,940 - Pf1N1B4_2747 0.86 -0.0
3,087,942 + Pf1N1B4_2747 0.86 +1.3
3,087,942 + Pf1N1B4_2747 0.86 +1.6
3,087,943 - Pf1N1B4_2747 0.86 +1.9
3,087,943 - Pf1N1B4_2747 0.86 +0.3
3,087,943 - Pf1N1B4_2747 0.86 +0.6
3,088,014 + +0.7
3,088,053 + +0.7
3,088,053 + -0.1
3,088,053 + -0.0
3,088,053 + +0.4
3,088,054 - -0.3
3,088,054 - -1.4
3,088,054 - -0.4
3,088,181 - -0.0
3,088,181 - -0.7
3,088,197 + +2.7
3,088,208 + +0.4
3,088,222 + +0.1
3,088,222 + -0.4
3,088,222 + -0.1
3,088,222 + -0.0
3,088,222 + -0.8
3,088,222 + -0.3
3,088,222 + -0.4
3,088,222 + -0.9
3,088,222 + +2.1
3,088,222 + -1.3
3,088,222 + -1.8
3,088,223 - -2.0
3,088,223 - -2.4
3,088,223 - -0.4
3,088,223 - -1.3
3,088,223 - -2.4
3,088,223 - -1.6
3,088,223 - -1.3
3,088,223 - -2.8
3,088,223 - +0.4
3,088,310 + -1.6
3,088,310 + -2.7
3,088,310 + -2.8
3,088,311 - -1.8
3,088,311 - -1.6
3,088,311 - -0.8
3,088,311 - +0.6
3,088,314 + +0.8
3,088,314 + -0.0
3,088,314 + -3.0
3,088,315 - -2.9
3,088,315 - -1.1
3,088,315 - -1.0
3,088,315 - -0.8
3,088,315 - +0.2
3,088,498 + Pf1N1B4_2748 0.29 -0.4
3,088,498 + Pf1N1B4_2748 0.29 -0.2
3,088,498 + Pf1N1B4_2748 0.29 -0.2
3,088,498 + Pf1N1B4_2748 0.29 -2.5
3,088,498 + Pf1N1B4_2748 0.29 -1.3
3,088,498 + Pf1N1B4_2748 0.29 -0.5
3,088,498 + Pf1N1B4_2748 0.29 -0.8
3,088,498 + Pf1N1B4_2748 0.29 -0.4
3,088,498 + Pf1N1B4_2748 0.29 -0.3
3,088,498 + Pf1N1B4_2748 0.29 -2.0
3,088,499 - Pf1N1B4_2748 0.29 -2.4
3,088,499 - Pf1N1B4_2748 0.29 +0.6
3,088,499 - Pf1N1B4_2748 0.29 -2.0
3,088,499 - Pf1N1B4_2748 0.29 -3.0
3,088,499 - Pf1N1B4_2748 0.29 +0.6
3,088,499 - Pf1N1B4_2748 0.29 -0.0
3,088,499 - Pf1N1B4_2748 0.29 -1.9
3,088,499 - Pf1N1B4_2748 0.29 -0.4
3,088,501 + Pf1N1B4_2748 0.29 -1.6
3,088,502 - Pf1N1B4_2748 0.29 -0.3
3,088,502 - Pf1N1B4_2748 0.29 -2.4
3,088,504 + Pf1N1B4_2748 0.30 -2.2
3,088,504 + Pf1N1B4_2748 0.30 +2.1
3,088,504 + Pf1N1B4_2748 0.30 -2.4
3,088,505 - Pf1N1B4_2748 0.30 -1.0
3,088,505 - Pf1N1B4_2748 0.30 -1.4
3,088,505 - Pf1N1B4_2748 0.30 -1.4
3,088,505 - Pf1N1B4_2748 0.30 -1.6
3,088,515 + Pf1N1B4_2748 0.31 -1.0
3,088,515 + Pf1N1B4_2748 0.31 -0.4
3,088,528 + Pf1N1B4_2748 0.33 -0.8
3,088,528 + Pf1N1B4_2748 0.33 -0.0
3,088,553 - Pf1N1B4_2748 0.37 -0.0
3,088,708 + Pf1N1B4_2748 0.60 -1.3
3,088,799 + Pf1N1B4_2748 0.73 -2.2
3,088,799 + Pf1N1B4_2748 0.73 -0.4
3,088,799 + Pf1N1B4_2748 0.73 -0.6
3,088,799 + Pf1N1B4_2748 0.73 -1.0
3,088,799 + Pf1N1B4_2748 0.73 -2.0
3,088,799 + Pf1N1B4_2748 0.73 +1.0
3,088,800 - Pf1N1B4_2748 0.73 -0.6
3,088,800 - Pf1N1B4_2748 0.73 -2.6
3,088,800 - Pf1N1B4_2748 0.73 -0.8
3,088,859 - Pf1N1B4_2748 0.82 -0.4
3,088,878 + Pf1N1B4_2748 0.85 -0.0
3,088,878 + Pf1N1B4_2748 0.85 -2.7
3,088,879 - Pf1N1B4_2748 0.85 +1.0
3,088,879 - Pf1N1B4_2748 0.85 -2.8
3,089,007 - -0.0
3,089,226 - Pf1N1B4_2749 0.20 -0.7
3,089,226 - Pf1N1B4_2749 0.20 -1.3
3,089,226 - Pf1N1B4_2749 0.20 -1.5
3,089,226 - Pf1N1B4_2749 0.20 +0.1
3,089,314 - Pf1N1B4_2749 0.30 -0.9
3,089,455 + Pf1N1B4_2749 0.47 +0.7
3,089,455 + Pf1N1B4_2749 0.47 -2.5
3,089,456 - Pf1N1B4_2749 0.47 +2.1
3,089,456 - Pf1N1B4_2749 0.47 -0.5
3,089,456 - Pf1N1B4_2749 0.47 -0.2
3,089,456 - Pf1N1B4_2749 0.47 -0.3
3,089,471 - Pf1N1B4_2749 0.49 -0.0
3,089,515 + Pf1N1B4_2749 0.54 -1.3
3,089,516 - Pf1N1B4_2749 0.54 -1.9
3,089,516 - Pf1N1B4_2749 0.54 -0.6
3,089,805 - Pf1N1B4_2749 0.88 +0.5

Or see this region's nucleotide sequence