Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_245 and Pf1N1B4_246 are separated by 1 nucleotides Pf1N1B4_246 and Pf1N1B4_247 overlap by 4 nucleotides Pf1N1B4_247 and Pf1N1B4_248 are separated by 123 nucleotides Pf1N1B4_248 and Pf1N1B4_249 are separated by 67 nucleotides
Pf1N1B4_245 - Probable transmembrane protein, at 303,166 to 303,480
_245
Pf1N1B4_246 - Probable transmembrane protein, at 303,482 to 303,871
_246
Pf1N1B4_247 - FIG00956101: hypothetical protein, at 303,868 to 304,206
_247
Pf1N1B4_248 - EAL-domain protein, at 304,330 to 305,493
_248
Pf1N1B4_249 - Deoxyguanosinetriphosphate triphosphohydrolase (EC 3.1.5.1), at 305,561 to 306,892
_249
Position (kb) 304
305
306 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 303.397 kb on + strand, within Pf1N1B4_245 at 303.397 kb on + strand, within Pf1N1B4_245 at 303.482 kb on - strand at 303.482 kb on - strand at 303.716 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.753 kb on - strand, within Pf1N1B4_246 at 304.204 kb on + strand at 304.247 kb on + strand at 304.315 kb on + strand at 304.315 kb on + strand at 304.316 kb on - strand at 304.318 kb on + strand at 304.318 kb on + strand at 304.319 kb on - strand at 304.319 kb on - strand at 304.319 kb on - strand at 304.574 kb on + strand, within Pf1N1B4_248 at 304.575 kb on - strand, within Pf1N1B4_248 at 304.981 kb on + strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 305.072 kb on - strand at 305.314 kb on - strand, within Pf1N1B4_248 at 305.338 kb on + strand at 305.339 kb on - strand, within Pf1N1B4_248 at 305.393 kb on - strand at 305.491 kb on + strand at 305.492 kb on - strand at 305.492 kb on - strand at 306.033 kb on + strand, within Pf1N1B4_249 at 306.033 kb on + strand, within Pf1N1B4_249 at 306.038 kb on - strand, within Pf1N1B4_249 at 306.038 kb on - strand at 306.038 kb on - strand, within Pf1N1B4_249 at 306.050 kb on + strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.111 kb on - strand, within Pf1N1B4_249 at 306.263 kb on + strand, within Pf1N1B4_249 at 306.264 kb on - strand, within Pf1N1B4_249
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 303,397 + Pf1N1B4_245 0.73 -1.9 303,397 + Pf1N1B4_245 0.73 +0.8 303,482 - -0.0 303,482 - -0.9 303,716 + Pf1N1B4_246 0.60 -0.8 303,752 + Pf1N1B4_246 0.69 +0.1 303,752 + Pf1N1B4_246 0.69 -0.1 303,752 + Pf1N1B4_246 0.69 +0.1 303,752 + Pf1N1B4_246 0.69 +2.7 303,752 + Pf1N1B4_246 0.69 -0.2 303,752 + Pf1N1B4_246 0.69 -0.1 303,752 + Pf1N1B4_246 0.69 -0.3 303,752 + Pf1N1B4_246 0.69 -1.0 303,752 + Pf1N1B4_246 0.69 -0.4 303,752 + Pf1N1B4_246 0.69 +0.4 303,753 - Pf1N1B4_246 0.69 -1.2 304,204 + +0.2 304,247 + +0.9 304,315 + -0.0 304,315 + -0.5 304,316 - +0.1 304,318 + -2.2 304,318 + -0.4 304,319 - -1.6 304,319 - -1.0 304,319 - -0.4 304,574 + Pf1N1B4_248 0.21 +1.9 304,575 - Pf1N1B4_248 0.21 +0.0 304,981 + Pf1N1B4_248 0.56 +1.6 304,986 - Pf1N1B4_248 0.56 -0.4 304,986 - Pf1N1B4_248 0.56 +0.2 304,986 - Pf1N1B4_248 0.56 +0.4 305,072 - -0.4 305,314 - Pf1N1B4_248 0.85 +0.7 305,338 + +1.6 305,339 - Pf1N1B4_248 0.87 +0.8 305,393 - +0.0 305,491 + -0.2 305,492 - -1.2 305,492 - -0.4 306,033 + Pf1N1B4_249 0.35 -0.5 306,033 + Pf1N1B4_249 0.35 +1.0 306,038 - Pf1N1B4_249 0.36 -1.0 306,038 - -0.8 306,038 - Pf1N1B4_249 0.36 +0.6 306,050 + Pf1N1B4_249 0.37 -0.4 306,084 - Pf1N1B4_249 0.39 -0.5 306,084 - Pf1N1B4_249 0.39 +2.8 306,084 - Pf1N1B4_249 0.39 -0.2 306,101 + Pf1N1B4_249 0.41 +0.0 306,101 + Pf1N1B4_249 0.41 -0.7 306,101 + Pf1N1B4_249 0.41 -0.6 306,111 - Pf1N1B4_249 0.41 -0.2 306,263 + Pf1N1B4_249 0.53 -0.3 306,264 - Pf1N1B4_249 0.53 -1.0
Or see this region's nucleotide sequence