Experiment: Nitrate reduction, replicate C 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_2476 and Pf1N1B4_2477 are separated by 266 nucleotides Pf1N1B4_2477 and Pf1N1B4_2478 are separated by 173 nucleotides 
        Pf1N1B4_2476 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 2,807,554 to 2,809,494 
        _2476 
         
        
        Pf1N1B4_2477 - response regulator, at 2,809,761 to 2,810,213 
        _2477 
         
        
        Pf1N1B4_2478 - Malate synthase G (EC 2.3.3.9), at 2,810,387 to 2,812,564 
        _2478 
         Position (kb) 2809 
2810 
2811 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 
3 
4 at 2808.802 kb on + strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.901 kb on + strand, within Pf1N1B4_2476 at 2808.902 kb on - strand, within Pf1N1B4_2476 at 2809.104 kb on + strand, within Pf1N1B4_2476 at 2809.104 kb on + strand, within Pf1N1B4_2476 at 2809.114 kb on + strand at 2809.125 kb on + strand, within Pf1N1B4_2476 at 2809.125 kb on + strand, within Pf1N1B4_2476 at 2809.126 kb on - strand, within Pf1N1B4_2476 at 2809.280 kb on + strand, within Pf1N1B4_2476 at 2809.280 kb on + strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.334 kb on + strand at 2809.335 kb on - strand at 2809.335 kb on - strand at 2809.335 kb on - strand at 2809.364 kb on + strand at 2809.411 kb on + strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.489 kb on - strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.597 kb on + strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.619 kb on - strand at 2809.656 kb on + strand at 2809.656 kb on + strand at 2809.657 kb on - strand at 2809.657 kb on - strand at 2809.775 kb on + strand at 2809.775 kb on + strand at 2809.776 kb on - strand at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.129 kb on - strand, within Pf1N1B4_2477 at 2810.385 kb on - strand at 2810.657 kb on - strand, within Pf1N1B4_2478 at 2810.657 kb on - strand at 2810.657 kb on - strand, within Pf1N1B4_2478 at 2810.749 kb on - strand, within Pf1N1B4_2478 at 2810.907 kb on + strand, within Pf1N1B4_2478 at 2810.972 kb on - strand, within Pf1N1B4_2478  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction, replicate C remove 2,808,802 +  Pf1N1B4_2476 0.64  -0.6 2,808,803 -  Pf1N1B4_2476 0.64  -0.1 2,808,803 -  Pf1N1B4_2476 0.64  -0.5 2,808,803 -  Pf1N1B4_2476 0.64  +1.8 2,808,803 -  Pf1N1B4_2476 0.64  +0.8 2,808,803 -  Pf1N1B4_2476 0.64  +2.2 2,808,901 +  Pf1N1B4_2476 0.69  -1.7 2,808,902 -  Pf1N1B4_2476 0.69  +0.6 2,809,104 +  Pf1N1B4_2476 0.80  +2.2 2,809,104 +  Pf1N1B4_2476 0.80  +1.8 2,809,114 +  +0.5 2,809,125 +  Pf1N1B4_2476 0.81  +0.1 2,809,125 +  Pf1N1B4_2476 0.81  +0.7 2,809,126 -  Pf1N1B4_2476 0.81  +3.8 2,809,280 +  Pf1N1B4_2476 0.89  +1.2 2,809,280 +  Pf1N1B4_2476 0.89  +0.5 2,809,281 -  Pf1N1B4_2476 0.89  -1.7 2,809,281 -  Pf1N1B4_2476 0.89  -0.6 2,809,281 -  Pf1N1B4_2476 0.89  +1.5 2,809,334 +  +0.3 2,809,335 -  -0.5 2,809,335 -  -0.3 2,809,335 -  +0.5 2,809,364 +  +0.3 2,809,411 +  +4.1 2,809,412 -  -1.7 2,809,412 -  -0.8 2,809,412 -  -0.7 2,809,489 -  -2.1 2,809,587 +  +0.5 2,809,587 +  -0.7 2,809,587 +  +1.3 2,809,587 +  -0.1 2,809,587 +  +0.6 2,809,587 +  -1.1 2,809,587 +  +0.4 2,809,587 +  +2.1 2,809,588 -  +1.5 2,809,588 -  -1.3 2,809,588 -  -1.8 2,809,588 -  -1.3 2,809,588 -  +0.1 2,809,593 +  +0.7 2,809,593 +  +1.8 2,809,593 +  +1.5 2,809,594 -  +0.3 2,809,594 -  -0.1 2,809,594 -  -0.4 2,809,594 -  -1.8 2,809,594 -  -1.6 2,809,594 -  -1.1 2,809,594 -  +1.2 2,809,594 -  +0.5 2,809,594 -  -0.8 2,809,597 +  -0.1 2,809,598 -  -0.8 2,809,598 -  +0.8 2,809,598 -  -2.8 2,809,619 -  -2.4 2,809,656 +  -1.7 2,809,656 +  +0.6 2,809,657 -  +0.4 2,809,657 -  +0.0 2,809,775 +  -1.8 2,809,775 +  -1.5 2,809,776 -  -1.6 2,809,954 +  Pf1N1B4_2477 0.43  -2.3 2,809,954 +  Pf1N1B4_2477 0.43  -1.3 2,809,954 +  Pf1N1B4_2477 0.43  -1.4 2,809,954 +  Pf1N1B4_2477 0.43  -1.3 2,810,128 +  Pf1N1B4_2477 0.81  +0.9 2,810,128 +  Pf1N1B4_2477 0.81  -1.1 2,810,128 +  Pf1N1B4_2477 0.81  -0.8 2,810,129 -  Pf1N1B4_2477 0.81  -1.5 2,810,385 -  -1.7 2,810,657 -  Pf1N1B4_2478 0.12  -0.8 2,810,657 -  -0.1 2,810,657 -  Pf1N1B4_2478 0.12  -0.7 2,810,749 -  Pf1N1B4_2478 0.17  +0.0 2,810,907 +  Pf1N1B4_2478 0.24  +0.1 2,810,972 -  Pf1N1B4_2478 0.27  -0.6 
Or see this region's nucleotide sequence