Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2474 and Pf1N1B4_2475 are separated by 291 nucleotides Pf1N1B4_2475 and Pf1N1B4_2476 overlap by 4 nucleotides Pf1N1B4_2476 and Pf1N1B4_2477 are separated by 266 nucleotides Pf1N1B4_2477 and Pf1N1B4_2478 are separated by 173 nucleotides
Pf1N1B4_2474 - RNA polymerase sigma-70 factor, ECF subfamily, at 2,806,082 to 2,806,558
_2474
Pf1N1B4_2475 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 2,806,850 to 2,807,557
_2475
Pf1N1B4_2476 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 2,807,554 to 2,809,494
_2476
Pf1N1B4_2477 - response regulator, at 2,809,761 to 2,810,213
_2477
Pf1N1B4_2478 - Malate synthase G (EC 2.3.3.9), at 2,810,387 to 2,812,564
_2478
Position (kb)
2807
2808
2809
2810 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2806.554 kb on + strand at 2806.669 kb on + strand at 2806.669 kb on + strand at 2806.670 kb on - strand at 2806.851 kb on + strand at 2806.851 kb on + strand at 2806.851 kb on + strand at 2806.915 kb on + strand at 2806.916 kb on - strand at 2807.121 kb on + strand, within Pf1N1B4_2475 at 2807.122 kb on - strand, within Pf1N1B4_2475 at 2807.395 kb on + strand, within Pf1N1B4_2475 at 2807.429 kb on + strand, within Pf1N1B4_2475 at 2807.557 kb on + strand at 2807.557 kb on + strand at 2807.557 kb on + strand at 2807.558 kb on - strand at 2807.558 kb on - strand at 2807.558 kb on - strand at 2807.692 kb on + strand at 2807.693 kb on - strand at 2807.693 kb on - strand at 2807.732 kb on + strand at 2808.064 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.567 kb on + strand, within Pf1N1B4_2476 at 2808.568 kb on - strand, within Pf1N1B4_2476 at 2808.568 kb on - strand, within Pf1N1B4_2476 at 2808.568 kb on - strand, within Pf1N1B4_2476 at 2808.568 kb on - strand, within Pf1N1B4_2476 at 2808.728 kb on + strand, within Pf1N1B4_2476 at 2808.729 kb on - strand, within Pf1N1B4_2476 at 2808.802 kb on + strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.901 kb on + strand, within Pf1N1B4_2476 at 2808.902 kb on - strand, within Pf1N1B4_2476 at 2809.104 kb on + strand, within Pf1N1B4_2476 at 2809.104 kb on + strand, within Pf1N1B4_2476 at 2809.114 kb on + strand at 2809.125 kb on + strand, within Pf1N1B4_2476 at 2809.125 kb on + strand, within Pf1N1B4_2476 at 2809.126 kb on - strand, within Pf1N1B4_2476 at 2809.280 kb on + strand, within Pf1N1B4_2476 at 2809.280 kb on + strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.334 kb on + strand at 2809.335 kb on - strand at 2809.335 kb on - strand at 2809.335 kb on - strand at 2809.364 kb on + strand at 2809.411 kb on + strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.489 kb on - strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.597 kb on + strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.619 kb on - strand at 2809.656 kb on + strand at 2809.656 kb on + strand at 2809.657 kb on - strand at 2809.657 kb on - strand at 2809.775 kb on + strand at 2809.775 kb on + strand at 2809.776 kb on - strand at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.129 kb on - strand, within Pf1N1B4_2477 at 2810.385 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 2,806,554 + -0.9 2,806,669 + -1.0 2,806,669 + -0.2 2,806,670 - -1.1 2,806,851 + +0.2 2,806,851 + -1.3 2,806,851 + -0.1 2,806,915 + +0.5 2,806,916 - -0.5 2,807,121 + Pf1N1B4_2475 0.38 +0.2 2,807,122 - Pf1N1B4_2475 0.38 +0.5 2,807,395 + Pf1N1B4_2475 0.77 -0.1 2,807,429 + Pf1N1B4_2475 0.82 -0.5 2,807,557 + -0.7 2,807,557 + -0.1 2,807,557 + -2.4 2,807,558 - -1.8 2,807,558 - -1.1 2,807,558 - +0.9 2,807,692 + -0.5 2,807,693 - -0.4 2,807,693 - +1.0 2,807,732 + -0.1 2,808,064 + Pf1N1B4_2476 0.26 +0.6 2,808,080 + Pf1N1B4_2476 0.27 -0.5 2,808,080 + Pf1N1B4_2476 0.27 +0.8 2,808,080 + Pf1N1B4_2476 0.27 -1.3 2,808,080 + Pf1N1B4_2476 0.27 -0.3 2,808,081 - Pf1N1B4_2476 0.27 -0.1 2,808,081 - Pf1N1B4_2476 0.27 -0.5 2,808,081 - Pf1N1B4_2476 0.27 +0.0 2,808,081 - +2.0 2,808,081 - Pf1N1B4_2476 0.27 -1.7 2,808,081 - Pf1N1B4_2476 0.27 +1.1 2,808,081 - Pf1N1B4_2476 0.27 +0.7 2,808,567 + Pf1N1B4_2476 0.52 -1.4 2,808,568 - Pf1N1B4_2476 0.52 +0.1 2,808,568 - Pf1N1B4_2476 0.52 +1.4 2,808,568 - Pf1N1B4_2476 0.52 -1.8 2,808,568 - Pf1N1B4_2476 0.52 +0.1 2,808,728 + Pf1N1B4_2476 0.60 -2.2 2,808,729 - Pf1N1B4_2476 0.61 +0.5 2,808,802 + Pf1N1B4_2476 0.64 -0.6 2,808,803 - Pf1N1B4_2476 0.64 -0.1 2,808,803 - Pf1N1B4_2476 0.64 -0.5 2,808,803 - Pf1N1B4_2476 0.64 +1.8 2,808,803 - Pf1N1B4_2476 0.64 +0.8 2,808,803 - Pf1N1B4_2476 0.64 +2.2 2,808,901 + Pf1N1B4_2476 0.69 -1.7 2,808,902 - Pf1N1B4_2476 0.69 +0.6 2,809,104 + Pf1N1B4_2476 0.80 +2.2 2,809,104 + Pf1N1B4_2476 0.80 +1.8 2,809,114 + +0.5 2,809,125 + Pf1N1B4_2476 0.81 +0.1 2,809,125 + Pf1N1B4_2476 0.81 +0.7 2,809,126 - Pf1N1B4_2476 0.81 +3.8 2,809,280 + Pf1N1B4_2476 0.89 +1.2 2,809,280 + Pf1N1B4_2476 0.89 +0.5 2,809,281 - Pf1N1B4_2476 0.89 -1.7 2,809,281 - Pf1N1B4_2476 0.89 -0.6 2,809,281 - Pf1N1B4_2476 0.89 +1.5 2,809,334 + +0.3 2,809,335 - -0.5 2,809,335 - -0.3 2,809,335 - +0.5 2,809,364 + +0.3 2,809,411 + +4.1 2,809,412 - -1.7 2,809,412 - -0.8 2,809,412 - -0.7 2,809,489 - -2.1 2,809,587 + +0.5 2,809,587 + -0.7 2,809,587 + +1.3 2,809,587 + -0.1 2,809,587 + +0.6 2,809,587 + -1.1 2,809,587 + +0.4 2,809,587 + +2.1 2,809,588 - +1.5 2,809,588 - -1.3 2,809,588 - -1.8 2,809,588 - -1.3 2,809,588 - +0.1 2,809,593 + +0.7 2,809,593 + +1.8 2,809,593 + +1.5 2,809,594 - +0.3 2,809,594 - -0.1 2,809,594 - -0.4 2,809,594 - -1.8 2,809,594 - -1.6 2,809,594 - -1.1 2,809,594 - +1.2 2,809,594 - +0.5 2,809,594 - -0.8 2,809,597 + -0.1 2,809,598 - -0.8 2,809,598 - +0.8 2,809,598 - -2.8 2,809,619 - -2.4 2,809,656 + -1.7 2,809,656 + +0.6 2,809,657 - +0.4 2,809,657 - +0.0 2,809,775 + -1.8 2,809,775 + -1.5 2,809,776 - -1.6 2,809,954 + Pf1N1B4_2477 0.43 -2.3 2,809,954 + Pf1N1B4_2477 0.43 -1.3 2,809,954 + Pf1N1B4_2477 0.43 -1.4 2,809,954 + Pf1N1B4_2477 0.43 -1.3 2,810,128 + Pf1N1B4_2477 0.81 +0.9 2,810,128 + Pf1N1B4_2477 0.81 -1.1 2,810,128 + Pf1N1B4_2477 0.81 -0.8 2,810,129 - Pf1N1B4_2477 0.81 -1.5 2,810,385 - -1.7
Or see this region's nucleotide sequence