Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_240 and Pf1N1B4_241 are separated by 163 nucleotides Pf1N1B4_241 and Pf1N1B4_242 are separated by 192 nucleotides Pf1N1B4_242 and Pf1N1B4_243 are separated by 1 nucleotides
Pf1N1B4_240 - Transcriptional regulators, at 299,003 to 299,323
_240
Pf1N1B4_241 - Thiol-disulfide isomerase and thioredoxins, at 299,487 to 299,744
_241
Pf1N1B4_242 - Glutathione-regulated potassium-efflux system protein KefB, at 299,937 to 301,649
_242
Pf1N1B4_243 - Ammonium transporter, at 301,651 to 302,925
_243
Position (kb) 299
300
301
302 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 298.953 kb on - strand at 298.990 kb on + strand at 298.990 kb on + strand at 298.991 kb on - strand at 298.991 kb on - strand at 299.103 kb on + strand, within Pf1N1B4_240 at 299.103 kb on + strand, within Pf1N1B4_240 at 299.103 kb on + strand, within Pf1N1B4_240 at 299.189 kb on - strand, within Pf1N1B4_240 at 299.357 kb on + strand at 299.411 kb on - strand at 299.576 kb on + strand, within Pf1N1B4_241 at 299.587 kb on + strand, within Pf1N1B4_241 at 299.750 kb on + strand at 299.750 kb on + strand at 299.750 kb on + strand at 299.751 kb on - strand at 299.751 kb on - strand at 299.763 kb on + strand at 299.764 kb on - strand at 299.798 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.901 kb on - strand at 299.901 kb on - strand at 299.901 kb on - strand at 300.409 kb on + strand, within Pf1N1B4_242 at 300.456 kb on + strand, within Pf1N1B4_242 at 300.677 kb on - strand, within Pf1N1B4_242 at 300.677 kb on - strand, within Pf1N1B4_242 at 301.207 kb on + strand, within Pf1N1B4_242 at 301.207 kb on + strand, within Pf1N1B4_242 at 301.207 kb on + strand, within Pf1N1B4_242 at 301.208 kb on - strand, within Pf1N1B4_242 at 301.218 kb on + strand, within Pf1N1B4_242 at 301.218 kb on + strand, within Pf1N1B4_242 at 301.218 kb on + strand, within Pf1N1B4_242 at 301.219 kb on - strand, within Pf1N1B4_242 at 301.342 kb on + strand, within Pf1N1B4_242 at 301.384 kb on + strand, within Pf1N1B4_242 at 301.621 kb on - strand at 301.703 kb on + strand at 301.704 kb on - strand at 301.704 kb on - strand at 301.704 kb on - strand at 302.188 kb on + strand, within Pf1N1B4_243 at 302.456 kb on + strand, within Pf1N1B4_243 at 302.456 kb on + strand, within Pf1N1B4_243 at 302.456 kb on + strand, within Pf1N1B4_243 at 302.457 kb on - strand, within Pf1N1B4_243 at 302.457 kb on - strand, within Pf1N1B4_243 at 302.588 kb on + strand, within Pf1N1B4_243
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 298,953 - -0.4 298,990 + -0.8 298,990 + +0.8 298,991 - -0.8 298,991 - +3.3 299,103 + Pf1N1B4_240 0.31 +2.1 299,103 + Pf1N1B4_240 0.31 -1.4 299,103 + Pf1N1B4_240 0.31 -0.8 299,189 - Pf1N1B4_240 0.58 +0.6 299,357 + +0.8 299,411 - -0.6 299,576 + Pf1N1B4_241 0.34 -1.5 299,587 + Pf1N1B4_241 0.39 +0.8 299,750 + -1.5 299,750 + -0.7 299,750 + -1.4 299,751 - +0.2 299,751 - -0.4 299,763 + -0.8 299,764 - -0.4 299,798 + -0.0 299,900 + -0.4 299,900 + +0.9 299,900 + +0.1 299,900 + -0.6 299,900 + -1.2 299,901 - -0.1 299,901 - +0.8 299,901 - +0.2 300,409 + Pf1N1B4_242 0.28 -1.2 300,456 + Pf1N1B4_242 0.30 -1.9 300,677 - Pf1N1B4_242 0.43 +0.6 300,677 - Pf1N1B4_242 0.43 +0.7 301,207 + Pf1N1B4_242 0.74 -1.4 301,207 + Pf1N1B4_242 0.74 +1.4 301,207 + Pf1N1B4_242 0.74 +1.6 301,208 - Pf1N1B4_242 0.74 -0.3 301,218 + Pf1N1B4_242 0.75 -0.5 301,218 + Pf1N1B4_242 0.75 +0.7 301,218 + Pf1N1B4_242 0.75 +0.8 301,219 - Pf1N1B4_242 0.75 +0.6 301,342 + Pf1N1B4_242 0.82 +0.6 301,384 + Pf1N1B4_242 0.84 +1.6 301,621 - +0.1 301,703 + +0.3 301,704 - +2.7 301,704 - -1.0 301,704 - +0.6 302,188 + Pf1N1B4_243 0.42 -0.3 302,456 + Pf1N1B4_243 0.63 -0.9 302,456 + Pf1N1B4_243 0.63 -0.1 302,456 + Pf1N1B4_243 0.63 -0.6 302,457 - Pf1N1B4_243 0.63 -0.0 302,457 - Pf1N1B4_243 0.63 -0.0 302,588 + Pf1N1B4_243 0.73 -0.8
Or see this region's nucleotide sequence