Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_223 and Pf1N1B4_224 overlap by 4 nucleotides Pf1N1B4_224 and Pf1N1B4_225 are separated by 116 nucleotides
Pf1N1B4_223 - Citronellyl-CoA dehydrogenase, at 283,755 to 284,912
_223
Pf1N1B4_224 - Isohexenylglutaconyl-CoA hydratase, at 284,909 to 285,706
_224
Pf1N1B4_225 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 285,823 to 287,784
_225
Position (kb) 284
285
286 Strain fitness (log2 ratio) -2
-1
0
1
2 at 283.948 kb on - strand, within Pf1N1B4_223 at 283.948 kb on - strand, within Pf1N1B4_223 at 283.948 kb on - strand, within Pf1N1B4_223 at 283.954 kb on - strand, within Pf1N1B4_223 at 284.156 kb on - strand, within Pf1N1B4_223 at 284.196 kb on + strand, within Pf1N1B4_223 at 284.196 kb on + strand, within Pf1N1B4_223 at 284.197 kb on - strand at 284.197 kb on - strand, within Pf1N1B4_223 at 284.197 kb on - strand, within Pf1N1B4_223 at 284.332 kb on + strand, within Pf1N1B4_223 at 284.333 kb on - strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.521 kb on - strand, within Pf1N1B4_223 at 284.544 kb on + strand, within Pf1N1B4_223 at 284.653 kb on - strand, within Pf1N1B4_223 at 284.670 kb on + strand at 284.670 kb on + strand, within Pf1N1B4_223 at 284.670 kb on + strand, within Pf1N1B4_223 at 284.671 kb on - strand, within Pf1N1B4_223 at 284.671 kb on - strand, within Pf1N1B4_223 at 285.288 kb on + strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.806 kb on + strand at 285.806 kb on + strand at 285.806 kb on + strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.920 kb on + strand at 286.006 kb on + strand at 286.007 kb on - strand at 286.007 kb on - strand at 286.495 kb on + strand, within Pf1N1B4_225 at 286.495 kb on + strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.682 kb on - strand, within Pf1N1B4_225 at 286.682 kb on - strand, within Pf1N1B4_225
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 283,948 - Pf1N1B4_223 0.17 +0.6 283,948 - Pf1N1B4_223 0.17 +0.9 283,948 - Pf1N1B4_223 0.17 -0.3 283,954 - Pf1N1B4_223 0.17 -2.0 284,156 - Pf1N1B4_223 0.35 +0.7 284,196 + Pf1N1B4_223 0.38 -1.0 284,196 + Pf1N1B4_223 0.38 +0.7 284,197 - +1.6 284,197 - Pf1N1B4_223 0.38 -0.3 284,197 - Pf1N1B4_223 0.38 -1.0 284,332 + Pf1N1B4_223 0.50 +0.2 284,333 - Pf1N1B4_223 0.50 +0.1 284,520 + Pf1N1B4_223 0.66 -1.0 284,520 + Pf1N1B4_223 0.66 -1.4 284,520 + Pf1N1B4_223 0.66 +0.1 284,520 + Pf1N1B4_223 0.66 -1.0 284,521 - Pf1N1B4_223 0.66 -0.3 284,544 + Pf1N1B4_223 0.68 +1.2 284,653 - Pf1N1B4_223 0.78 -2.0 284,670 + -0.0 284,670 + Pf1N1B4_223 0.79 +0.6 284,670 + Pf1N1B4_223 0.79 +0.2 284,671 - Pf1N1B4_223 0.79 -1.8 284,671 - Pf1N1B4_223 0.79 -0.0 285,288 + Pf1N1B4_224 0.47 -0.5 285,289 - Pf1N1B4_224 0.48 -0.4 285,289 - Pf1N1B4_224 0.48 +2.3 285,289 - Pf1N1B4_224 0.48 +0.6 285,806 + -1.2 285,806 + -0.2 285,806 + -2.7 285,807 - -1.1 285,807 - -0.6 285,807 - +0.8 285,807 - +1.1 285,807 - -2.1 285,807 - -0.2 285,920 + -1.2 286,006 + -1.5 286,007 - +1.6 286,007 - -1.2 286,495 + Pf1N1B4_225 0.34 -0.6 286,495 + Pf1N1B4_225 0.34 +1.4 286,631 - Pf1N1B4_225 0.41 +1.8 286,631 - Pf1N1B4_225 0.41 -0.7 286,631 - Pf1N1B4_225 0.41 +0.8 286,631 - Pf1N1B4_225 0.41 +0.7 286,682 - Pf1N1B4_225 0.44 +0.6 286,682 - Pf1N1B4_225 0.44 +2.2
Or see this region's nucleotide sequence