Experiment: Nitrate reduction, replicate C 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_2185 and Pf1N1B4_2186 overlap by 4 nucleotides Pf1N1B4_2186 and Pf1N1B4_2187 are separated by 16 nucleotides Pf1N1B4_2187 and Pf1N1B4_2188 overlap by 4 nucleotides 
        Pf1N1B4_2185 - Porphobilinogen deaminase (EC 2.5.1.61), at 2,497,352 to 2,498,293 
        _2185 
         
        
        Pf1N1B4_2186 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,498,290 to 2,499,057 
        _2186 
         
        
        Pf1N1B4_2187 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,499,074 to 2,500,228 
        _2187 
         
        
        Pf1N1B4_2188 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 2,500,225 to 2,501,463 
        _2188 
         Position (kb) 2499 
2500 
2501 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 at 2498.270 kb on + strand at 2498.270 kb on + strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2499.071 kb on + strand at 2500.881 kb on - strand, within Pf1N1B4_2188 at 2501.047 kb on + strand, within Pf1N1B4_2188 at 2501.122 kb on + strand, within Pf1N1B4_2188 at 2501.122 kb on + strand, within Pf1N1B4_2188  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction, replicate C remove 2,498,270 +  -1.7 2,498,270 +  -0.8 2,498,271 -  +0.5 2,498,271 -  +0.7 2,498,271 -  -2.8 2,498,271 -  +0.2 2,499,071 +  -0.2 2,500,881 -  Pf1N1B4_2188 0.53  +0.4 2,501,047 +  Pf1N1B4_2188 0.66  -0.5 2,501,122 +  Pf1N1B4_2188 0.72  +1.5 2,501,122 +  Pf1N1B4_2188 0.72  +0.8 
Or see this region's nucleotide sequence