Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_217 and Pf1N1B4_218 are separated by 112 nucleotides Pf1N1B4_218 and Pf1N1B4_219 are separated by 144 nucleotides Pf1N1B4_219 and Pf1N1B4_220 are separated by 178 nucleotides
Pf1N1B4_217 - Hypothetical protein PvdY @ Siderophore synthetase small component, acetyltransferase, at 276,453 to 277,466
_217
Pf1N1B4_218 - Periplasmic binding protein, at 277,579 to 278,322
_218
Pf1N1B4_219 - Acyclic terpenes utilization regulator AtuR, TetR family, at 278,467 to 279,093
_219
Pf1N1B4_220 - 3-hydroxy-3isohexenylglutaryl-CoA:acetate lyase, at 279,272 to 281,074
_220
Position (kb) 277
278
279 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 276.640 kb on + strand, within Pf1N1B4_217 at 276.641 kb on - strand, within Pf1N1B4_217 at 276.641 kb on - strand, within Pf1N1B4_217 at 276.743 kb on - strand, within Pf1N1B4_217 at 276.743 kb on - strand, within Pf1N1B4_217 at 276.743 kb on - strand at 276.743 kb on - strand, within Pf1N1B4_217 at 276.743 kb on - strand at 276.743 kb on - strand, within Pf1N1B4_217 at 276.775 kb on + strand, within Pf1N1B4_217 at 276.776 kb on - strand, within Pf1N1B4_217 at 276.776 kb on - strand, within Pf1N1B4_217 at 276.787 kb on + strand, within Pf1N1B4_217 at 276.788 kb on - strand, within Pf1N1B4_217 at 276.788 kb on - strand, within Pf1N1B4_217 at 276.831 kb on - strand at 276.831 kb on - strand, within Pf1N1B4_217 at 276.860 kb on - strand, within Pf1N1B4_217 at 276.961 kb on - strand, within Pf1N1B4_217 at 277.081 kb on + strand, within Pf1N1B4_217 at 277.588 kb on - strand at 277.614 kb on - strand at 277.614 kb on - strand at 277.725 kb on - strand, within Pf1N1B4_218 at 277.725 kb on - strand, within Pf1N1B4_218 at 277.799 kb on + strand, within Pf1N1B4_218 at 277.799 kb on + strand, within Pf1N1B4_218 at 277.800 kb on - strand, within Pf1N1B4_218 at 277.868 kb on + strand, within Pf1N1B4_218 at 277.868 kb on + strand, within Pf1N1B4_218 at 277.869 kb on - strand, within Pf1N1B4_218 at 277.869 kb on - strand, within Pf1N1B4_218 at 277.869 kb on - strand, within Pf1N1B4_218 at 277.886 kb on + strand, within Pf1N1B4_218 at 277.886 kb on + strand at 277.886 kb on + strand, within Pf1N1B4_218 at 277.886 kb on + strand, within Pf1N1B4_218 at 277.887 kb on - strand at 277.887 kb on - strand, within Pf1N1B4_218 at 277.887 kb on - strand, within Pf1N1B4_218 at 277.887 kb on - strand at 277.887 kb on - strand at 277.902 kb on - strand, within Pf1N1B4_218 at 277.902 kb on - strand, within Pf1N1B4_218 at 277.902 kb on - strand at 277.935 kb on - strand, within Pf1N1B4_218 at 277.985 kb on + strand, within Pf1N1B4_218 at 277.985 kb on + strand, within Pf1N1B4_218 at 277.986 kb on - strand, within Pf1N1B4_218 at 277.986 kb on - strand, within Pf1N1B4_218 at 277.986 kb on - strand, within Pf1N1B4_218 at 278.255 kb on + strand at 278.256 kb on - strand at 278.256 kb on - strand at 278.385 kb on - strand at 278.385 kb on - strand at 278.440 kb on - strand at 278.660 kb on + strand, within Pf1N1B4_219 at 278.661 kb on - strand, within Pf1N1B4_219 at 278.664 kb on + strand, within Pf1N1B4_219 at 278.720 kb on + strand, within Pf1N1B4_219 at 278.721 kb on - strand, within Pf1N1B4_219 at 278.721 kb on - strand at 278.721 kb on - strand, within Pf1N1B4_219 at 278.849 kb on + strand, within Pf1N1B4_219 at 278.914 kb on - strand, within Pf1N1B4_219 at 278.969 kb on + strand, within Pf1N1B4_219 at 279.106 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 276,640 + Pf1N1B4_217 0.18 +0.2 276,641 - Pf1N1B4_217 0.19 -0.8 276,641 - Pf1N1B4_217 0.19 -0.1 276,743 - Pf1N1B4_217 0.29 -0.5 276,743 - Pf1N1B4_217 0.29 +0.3 276,743 - -0.6 276,743 - Pf1N1B4_217 0.29 -0.3 276,743 - -0.8 276,743 - Pf1N1B4_217 0.29 +1.0 276,775 + Pf1N1B4_217 0.32 -0.2 276,776 - Pf1N1B4_217 0.32 -1.0 276,776 - Pf1N1B4_217 0.32 +1.0 276,787 + Pf1N1B4_217 0.33 -2.1 276,788 - Pf1N1B4_217 0.33 +0.6 276,788 - Pf1N1B4_217 0.33 +3.2 276,831 - +1.6 276,831 - Pf1N1B4_217 0.37 -0.6 276,860 - Pf1N1B4_217 0.40 +3.2 276,961 - Pf1N1B4_217 0.50 -1.5 277,081 + Pf1N1B4_217 0.62 -0.2 277,588 - +0.9 277,614 - -0.4 277,614 - +0.7 277,725 - Pf1N1B4_218 0.20 +2.0 277,725 - Pf1N1B4_218 0.20 +2.3 277,799 + Pf1N1B4_218 0.30 +2.3 277,799 + Pf1N1B4_218 0.30 +2.3 277,800 - Pf1N1B4_218 0.30 +2.4 277,868 + Pf1N1B4_218 0.39 +2.4 277,868 + Pf1N1B4_218 0.39 +2.5 277,869 - Pf1N1B4_218 0.39 +1.6 277,869 - Pf1N1B4_218 0.39 +2.3 277,869 - Pf1N1B4_218 0.39 +1.9 277,886 + Pf1N1B4_218 0.41 +1.2 277,886 + +2.2 277,886 + Pf1N1B4_218 0.41 +1.9 277,886 + Pf1N1B4_218 0.41 +1.9 277,887 - +0.8 277,887 - Pf1N1B4_218 0.41 +1.2 277,887 - Pf1N1B4_218 0.41 +2.3 277,887 - +1.9 277,887 - +0.9 277,902 - Pf1N1B4_218 0.43 +2.0 277,902 - Pf1N1B4_218 0.43 +1.2 277,902 - +2.1 277,935 - Pf1N1B4_218 0.48 +2.5 277,985 + Pf1N1B4_218 0.55 +2.0 277,985 + Pf1N1B4_218 0.55 +1.4 277,986 - Pf1N1B4_218 0.55 +2.0 277,986 - Pf1N1B4_218 0.55 +2.2 277,986 - Pf1N1B4_218 0.55 +2.2 278,255 + +1.8 278,256 - +2.3 278,256 - +2.6 278,385 - +1.7 278,385 - +1.6 278,440 - +0.2 278,660 + Pf1N1B4_219 0.31 -0.7 278,661 - Pf1N1B4_219 0.31 +0.6 278,664 + Pf1N1B4_219 0.31 -2.4 278,720 + Pf1N1B4_219 0.40 +0.2 278,721 - Pf1N1B4_219 0.41 -1.9 278,721 - +0.2 278,721 - Pf1N1B4_219 0.41 -1.4 278,849 + Pf1N1B4_219 0.61 -1.0 278,914 - Pf1N1B4_219 0.71 +0.6 278,969 + Pf1N1B4_219 0.80 -2.1 279,106 - -0.0
Or see this region's nucleotide sequence