Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1982 and Pf1N1B4_1983 are separated by 131 nucleotides Pf1N1B4_1983 and Pf1N1B4_1984 are separated by 78 nucleotides Pf1N1B4_1984 and Pf1N1B4_1985 are separated by 31 nucleotides
Pf1N1B4_1982 - Cardiolipin synthetase (EC 2.7.8.-), at 2,267,791 to 2,269,230
_1982
Pf1N1B4_1983 - FIG00953800: hypothetical protein, at 2,269,362 to 2,269,883
_1983
Pf1N1B4_1984 - Threonyl-tRNA synthetase (EC 6.1.1.3), at 2,269,962 to 2,270,864
_1984
Pf1N1B4_1985 - Dihydroorotase (EC 3.5.2.3), at 2,270,896 to 2,272,221
_1985
Position (kb)
2269
2270
2271 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2269.033 kb on - strand, within Pf1N1B4_1982 at 2269.033 kb on - strand, within Pf1N1B4_1982 at 2269.034 kb on + strand, within Pf1N1B4_1982 at 2269.034 kb on + strand, within Pf1N1B4_1982 at 2269.034 kb on + strand, within Pf1N1B4_1982 at 2269.034 kb on + strand, within Pf1N1B4_1982 at 2269.034 kb on + strand, within Pf1N1B4_1982 at 2269.034 kb on + strand, within Pf1N1B4_1982 at 2269.034 kb on + strand, within Pf1N1B4_1982 at 2269.034 kb on + strand, within Pf1N1B4_1982 at 2269.034 kb on + strand, within Pf1N1B4_1982 at 2269.034 kb on + strand, within Pf1N1B4_1982 at 2269.034 kb on + strand, within Pf1N1B4_1982 at 2269.035 kb on - strand, within Pf1N1B4_1982 at 2269.035 kb on - strand, within Pf1N1B4_1982 at 2269.035 kb on - strand, within Pf1N1B4_1982 at 2269.035 kb on - strand, within Pf1N1B4_1982 at 2269.035 kb on - strand, within Pf1N1B4_1982 at 2269.035 kb on - strand, within Pf1N1B4_1982 at 2269.223 kb on + strand at 2269.223 kb on + strand at 2269.223 kb on + strand at 2269.224 kb on - strand at 2269.224 kb on - strand at 2269.224 kb on - strand at 2269.262 kb on + strand at 2269.263 kb on - strand at 2269.263 kb on - strand at 2269.296 kb on + strand at 2269.296 kb on + strand at 2269.296 kb on + strand at 2269.296 kb on + strand at 2269.296 kb on + strand at 2269.297 kb on - strand at 2269.297 kb on - strand at 2269.297 kb on - strand at 2269.297 kb on - strand at 2269.342 kb on + strand at 2269.357 kb on - strand at 2269.522 kb on + strand, within Pf1N1B4_1983 at 2269.522 kb on + strand, within Pf1N1B4_1983 at 2269.523 kb on - strand, within Pf1N1B4_1983 at 2269.523 kb on - strand, within Pf1N1B4_1983 at 2269.578 kb on - strand, within Pf1N1B4_1983 at 2269.664 kb on + strand, within Pf1N1B4_1983 at 2269.664 kb on + strand, within Pf1N1B4_1983 at 2269.664 kb on + strand, within Pf1N1B4_1983 at 2269.665 kb on - strand, within Pf1N1B4_1983 at 2269.665 kb on - strand, within Pf1N1B4_1983 at 2269.665 kb on - strand, within Pf1N1B4_1983 at 2269.665 kb on - strand, within Pf1N1B4_1983 at 2269.685 kb on + strand at 2269.821 kb on - strand at 2269.829 kb on + strand, within Pf1N1B4_1983 at 2269.830 kb on - strand, within Pf1N1B4_1983 at 2269.918 kb on + strand at 2269.919 kb on - strand at 2270.089 kb on - strand, within Pf1N1B4_1984 at 2270.099 kb on - strand, within Pf1N1B4_1984 at 2270.099 kb on - strand, within Pf1N1B4_1984 at 2270.147 kb on + strand, within Pf1N1B4_1984 at 2270.302 kb on + strand, within Pf1N1B4_1984 at 2270.302 kb on + strand, within Pf1N1B4_1984 at 2270.355 kb on + strand, within Pf1N1B4_1984 at 2270.355 kb on + strand, within Pf1N1B4_1984 at 2270.356 kb on - strand, within Pf1N1B4_1984 at 2270.356 kb on - strand, within Pf1N1B4_1984 at 2270.530 kb on - strand, within Pf1N1B4_1984 at 2270.530 kb on - strand, within Pf1N1B4_1984 at 2270.530 kb on - strand, within Pf1N1B4_1984 at 2270.554 kb on + strand, within Pf1N1B4_1984 at 2270.555 kb on - strand, within Pf1N1B4_1984 at 2270.571 kb on + strand, within Pf1N1B4_1984 at 2270.572 kb on - strand, within Pf1N1B4_1984 at 2270.690 kb on + strand, within Pf1N1B4_1984 at 2270.787 kb on - strand at 2270.803 kb on + strand at 2270.803 kb on + strand at 2270.803 kb on + strand at 2270.803 kb on + strand at 2270.803 kb on + strand at 2270.803 kb on + strand at 2270.803 kb on + strand at 2270.804 kb on - strand at 2270.804 kb on - strand at 2270.824 kb on + strand at 2270.824 kb on + strand at 2270.824 kb on + strand at 2270.824 kb on + strand at 2270.825 kb on - strand at 2270.825 kb on - strand at 2270.904 kb on + strand at 2270.904 kb on + strand at 2270.905 kb on - strand at 2270.905 kb on - strand at 2270.912 kb on - strand at 2270.917 kb on + strand at 2270.937 kb on + strand at 2270.937 kb on + strand at 2270.938 kb on - strand at 2270.938 kb on - strand at 2270.979 kb on + strand at 2270.980 kb on - strand at 2270.980 kb on - strand at 2271.082 kb on - strand, within Pf1N1B4_1985 at 2271.089 kb on - strand, within Pf1N1B4_1985 at 2271.104 kb on + strand, within Pf1N1B4_1985 at 2271.104 kb on + strand, within Pf1N1B4_1985 at 2271.104 kb on + strand, within Pf1N1B4_1985 at 2271.193 kb on + strand, within Pf1N1B4_1985 at 2271.193 kb on + strand, within Pf1N1B4_1985 at 2271.270 kb on - strand, within Pf1N1B4_1985 at 2271.270 kb on - strand, within Pf1N1B4_1985 at 2271.294 kb on + strand, within Pf1N1B4_1985 at 2271.294 kb on + strand, within Pf1N1B4_1985 at 2271.295 kb on - strand, within Pf1N1B4_1985 at 2271.295 kb on - strand, within Pf1N1B4_1985 at 2271.529 kb on + strand, within Pf1N1B4_1985 at 2271.530 kb on - strand, within Pf1N1B4_1985 at 2271.530 kb on - strand, within Pf1N1B4_1985
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 2,269,033 - Pf1N1B4_1982 0.86 -1.9 2,269,033 - Pf1N1B4_1982 0.86 +0.4 2,269,034 + Pf1N1B4_1982 0.86 -0.8 2,269,034 + Pf1N1B4_1982 0.86 -0.6 2,269,034 + Pf1N1B4_1982 0.86 -0.7 2,269,034 + Pf1N1B4_1982 0.86 +0.2 2,269,034 + Pf1N1B4_1982 0.86 -1.4 2,269,034 + Pf1N1B4_1982 0.86 +1.1 2,269,034 + Pf1N1B4_1982 0.86 -0.8 2,269,034 + Pf1N1B4_1982 0.86 -0.7 2,269,034 + Pf1N1B4_1982 0.86 -0.6 2,269,034 + Pf1N1B4_1982 0.86 +0.7 2,269,034 + Pf1N1B4_1982 0.86 -0.3 2,269,035 - Pf1N1B4_1982 0.86 -2.3 2,269,035 - Pf1N1B4_1982 0.86 -0.3 2,269,035 - Pf1N1B4_1982 0.86 +0.3 2,269,035 - Pf1N1B4_1982 0.86 +0.1 2,269,035 - Pf1N1B4_1982 0.86 -0.6 2,269,035 - Pf1N1B4_1982 0.86 +0.4 2,269,223 + -1.4 2,269,223 + -0.3 2,269,223 + +0.2 2,269,224 - -1.6 2,269,224 - +0.4 2,269,224 - +1.6 2,269,262 + -1.8 2,269,263 - -0.9 2,269,263 - -1.3 2,269,296 + -1.5 2,269,296 + -1.4 2,269,296 + -0.6 2,269,296 + +0.2 2,269,296 + +1.6 2,269,297 - -0.6 2,269,297 - +0.3 2,269,297 - -0.0 2,269,297 - -0.8 2,269,342 + -0.2 2,269,357 - +0.3 2,269,522 + Pf1N1B4_1983 0.31 -2.0 2,269,522 + Pf1N1B4_1983 0.31 -0.4 2,269,523 - Pf1N1B4_1983 0.31 -0.4 2,269,523 - Pf1N1B4_1983 0.31 +1.7 2,269,578 - Pf1N1B4_1983 0.41 +0.6 2,269,664 + Pf1N1B4_1983 0.58 -0.6 2,269,664 + Pf1N1B4_1983 0.58 +0.2 2,269,664 + Pf1N1B4_1983 0.58 -0.4 2,269,665 - Pf1N1B4_1983 0.58 -1.0 2,269,665 - Pf1N1B4_1983 0.58 +0.9 2,269,665 - Pf1N1B4_1983 0.58 -0.1 2,269,665 - Pf1N1B4_1983 0.58 +0.8 2,269,685 + -0.0 2,269,821 - +1.0 2,269,829 + Pf1N1B4_1983 0.89 -0.4 2,269,830 - Pf1N1B4_1983 0.90 +0.1 2,269,918 + -1.9 2,269,919 - -0.3 2,270,089 - Pf1N1B4_1984 0.14 -0.4 2,270,099 - Pf1N1B4_1984 0.15 -0.0 2,270,099 - Pf1N1B4_1984 0.15 +1.7 2,270,147 + Pf1N1B4_1984 0.20 +2.9 2,270,302 + Pf1N1B4_1984 0.38 +0.3 2,270,302 + Pf1N1B4_1984 0.38 -1.0 2,270,355 + Pf1N1B4_1984 0.44 -1.9 2,270,355 + Pf1N1B4_1984 0.44 +1.2 2,270,356 - Pf1N1B4_1984 0.44 -0.4 2,270,356 - Pf1N1B4_1984 0.44 +2.6 2,270,530 - Pf1N1B4_1984 0.63 +0.9 2,270,530 - Pf1N1B4_1984 0.63 -1.4 2,270,530 - Pf1N1B4_1984 0.63 +0.8 2,270,554 + Pf1N1B4_1984 0.66 -0.0 2,270,555 - Pf1N1B4_1984 0.66 -2.4 2,270,571 + Pf1N1B4_1984 0.67 +0.7 2,270,572 - Pf1N1B4_1984 0.68 +0.4 2,270,690 + Pf1N1B4_1984 0.81 -1.4 2,270,787 - -0.0 2,270,803 + -0.0 2,270,803 + +0.7 2,270,803 + +0.2 2,270,803 + +1.6 2,270,803 + -1.1 2,270,803 + -0.0 2,270,803 + -1.3 2,270,804 - -1.2 2,270,804 - +0.2 2,270,824 + -1.1 2,270,824 + +0.7 2,270,824 + -0.3 2,270,824 + -1.9 2,270,825 - +1.0 2,270,825 - +0.6 2,270,904 + +0.0 2,270,904 + +1.6 2,270,905 - +0.2 2,270,905 - +0.6 2,270,912 - +1.2 2,270,917 + -1.0 2,270,937 + +0.9 2,270,937 + -0.9 2,270,938 - +2.8 2,270,938 - -0.8 2,270,979 + -1.0 2,270,980 - +0.7 2,270,980 - -0.4 2,271,082 - Pf1N1B4_1985 0.14 -0.0 2,271,089 - Pf1N1B4_1985 0.15 -0.4 2,271,104 + Pf1N1B4_1985 0.16 +0.3 2,271,104 + Pf1N1B4_1985 0.16 +0.7 2,271,104 + Pf1N1B4_1985 0.16 -1.3 2,271,193 + Pf1N1B4_1985 0.22 -1.0 2,271,193 + Pf1N1B4_1985 0.22 +0.1 2,271,270 - Pf1N1B4_1985 0.28 +2.1 2,271,270 - Pf1N1B4_1985 0.28 -0.4 2,271,294 + Pf1N1B4_1985 0.30 -0.4 2,271,294 + Pf1N1B4_1985 0.30 +1.6 2,271,295 - Pf1N1B4_1985 0.30 -2.1 2,271,295 - Pf1N1B4_1985 0.30 +1.4 2,271,529 + Pf1N1B4_1985 0.48 -1.9 2,271,530 - Pf1N1B4_1985 0.48 -1.9 2,271,530 - Pf1N1B4_1985 0.48 -0.9
Or see this region's nucleotide sequence