Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1974 and Pf1N1B4_1975 are separated by 146 nucleotides Pf1N1B4_1975 and Pf1N1B4_1976 are separated by 122 nucleotides Pf1N1B4_1976 and Pf1N1B4_1977 are separated by 190 nucleotides
Pf1N1B4_1974 - L-lysine permease, at 2,257,448 to 2,258,077
_1974
Pf1N1B4_1975 - Transcriptional regulator, AsnC family, at 2,258,224 to 2,258,697
_1975
Pf1N1B4_1976 - Dihydrolipoamide dehydrogenase (EC 1.8.1.4), at 2,258,820 to 2,260,220
_1976
Pf1N1B4_1977 - Membrane protease subunits, stomatin/prohibitin homologs, at 2,260,411 to 2,262,396
_1977
Position (kb)
2258
2259
2260
2261 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2257.941 kb on - strand, within Pf1N1B4_1974 at 2258.034 kb on + strand at 2258.034 kb on + strand at 2258.035 kb on - strand at 2258.035 kb on - strand at 2258.035 kb on - strand at 2258.035 kb on - strand at 2258.117 kb on + strand at 2258.117 kb on + strand at 2258.117 kb on + strand at 2258.117 kb on + strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.179 kb on - strand at 2258.575 kb on + strand, within Pf1N1B4_1975 at 2258.576 kb on - strand, within Pf1N1B4_1975 at 2258.576 kb on - strand, within Pf1N1B4_1975 at 2258.576 kb on - strand, within Pf1N1B4_1975 at 2258.695 kb on + strand at 2258.695 kb on + strand at 2258.695 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2259.024 kb on + strand, within Pf1N1B4_1976 at 2259.024 kb on + strand, within Pf1N1B4_1976 at 2259.024 kb on + strand, within Pf1N1B4_1976 at 2259.024 kb on + strand at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.098 kb on + strand, within Pf1N1B4_1976 at 2259.098 kb on + strand, within Pf1N1B4_1976 at 2259.100 kb on + strand, within Pf1N1B4_1976 at 2259.100 kb on + strand, within Pf1N1B4_1976 at 2259.100 kb on + strand, within Pf1N1B4_1976 at 2259.100 kb on + strand, within Pf1N1B4_1976 at 2259.101 kb on - strand, within Pf1N1B4_1976 at 2259.101 kb on - strand, within Pf1N1B4_1976 at 2259.101 kb on - strand, within Pf1N1B4_1976 at 2259.166 kb on + strand, within Pf1N1B4_1976 at 2259.166 kb on + strand, within Pf1N1B4_1976 at 2259.166 kb on + strand, within Pf1N1B4_1976 at 2259.166 kb on + strand, within Pf1N1B4_1976 at 2259.167 kb on - strand, within Pf1N1B4_1976 at 2259.190 kb on + strand, within Pf1N1B4_1976 at 2259.190 kb on + strand, within Pf1N1B4_1976 at 2259.190 kb on + strand, within Pf1N1B4_1976 at 2259.190 kb on + strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.389 kb on + strand, within Pf1N1B4_1976 at 2259.389 kb on + strand, within Pf1N1B4_1976 at 2259.390 kb on - strand, within Pf1N1B4_1976 at 2259.390 kb on - strand, within Pf1N1B4_1976 at 2259.390 kb on - strand, within Pf1N1B4_1976 at 2259.390 kb on - strand, within Pf1N1B4_1976 at 2259.391 kb on + strand, within Pf1N1B4_1976 at 2259.391 kb on + strand, within Pf1N1B4_1976 at 2259.391 kb on + strand, within Pf1N1B4_1976 at 2259.391 kb on + strand, within Pf1N1B4_1976 at 2259.391 kb on + strand, within Pf1N1B4_1976 at 2259.392 kb on - strand, within Pf1N1B4_1976 at 2259.421 kb on + strand, within Pf1N1B4_1976 at 2259.422 kb on - strand, within Pf1N1B4_1976 at 2259.422 kb on - strand, within Pf1N1B4_1976 at 2259.422 kb on - strand, within Pf1N1B4_1976 at 2260.042 kb on + strand, within Pf1N1B4_1976 at 2260.042 kb on + strand, within Pf1N1B4_1976 at 2260.042 kb on + strand, within Pf1N1B4_1976 at 2260.043 kb on - strand, within Pf1N1B4_1976 at 2260.043 kb on - strand, within Pf1N1B4_1976 at 2260.043 kb on - strand, within Pf1N1B4_1976 at 2260.172 kb on + strand at 2260.173 kb on - strand at 2260.173 kb on - strand at 2260.173 kb on - strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.229 kb on + strand at 2260.229 kb on + strand at 2260.230 kb on - strand at 2260.230 kb on - strand at 2260.230 kb on - strand at 2260.230 kb on - strand at 2260.263 kb on + strand at 2260.263 kb on + strand at 2260.263 kb on + strand at 2260.263 kb on + strand at 2260.263 kb on + strand at 2260.264 kb on - strand at 2260.264 kb on - strand at 2260.280 kb on + strand at 2260.281 kb on - strand at 2260.288 kb on + strand at 2260.288 kb on + strand at 2260.288 kb on + strand at 2260.288 kb on + strand at 2260.288 kb on + strand at 2260.289 kb on - strand at 2260.289 kb on - strand at 2260.289 kb on - strand at 2260.289 kb on - strand at 2260.291 kb on + strand at 2260.350 kb on + strand at 2260.350 kb on + strand at 2260.350 kb on + strand at 2260.350 kb on + strand at 2260.350 kb on + strand at 2260.350 kb on + strand at 2260.351 kb on - strand at 2260.351 kb on - strand at 2260.351 kb on - strand at 2260.351 kb on - strand at 2260.354 kb on + strand at 2260.354 kb on + strand at 2260.354 kb on + strand at 2260.354 kb on + strand at 2260.355 kb on - strand at 2260.355 kb on - strand at 2260.364 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.374 kb on - strand at 2260.374 kb on - strand at 2260.374 kb on - strand at 2260.374 kb on - strand at 2260.374 kb on - strand at 2260.374 kb on - strand at 2260.432 kb on - strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.475 kb on + strand at 2260.762 kb on + strand, within Pf1N1B4_1977 at 2260.762 kb on + strand, within Pf1N1B4_1977 at 2260.832 kb on + strand, within Pf1N1B4_1977 at 2260.864 kb on + strand, within Pf1N1B4_1977 at 2260.864 kb on + strand, within Pf1N1B4_1977 at 2260.864 kb on + strand, within Pf1N1B4_1977 at 2260.865 kb on - strand, within Pf1N1B4_1977 at 2260.865 kb on - strand, within Pf1N1B4_1977 at 2261.084 kb on + strand, within Pf1N1B4_1977 at 2261.084 kb on + strand, within Pf1N1B4_1977 at 2261.084 kb on + strand, within Pf1N1B4_1977 at 2261.085 kb on - strand, within Pf1N1B4_1977 at 2261.085 kb on - strand, within Pf1N1B4_1977 at 2261.085 kb on - strand, within Pf1N1B4_1977 at 2261.085 kb on - strand, within Pf1N1B4_1977
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 2,257,941 - Pf1N1B4_1974 0.78 -0.7 2,258,034 + -0.9 2,258,034 + -0.5 2,258,035 - -0.3 2,258,035 - -1.3 2,258,035 - -1.0 2,258,035 - +1.4 2,258,117 + +0.5 2,258,117 + -1.1 2,258,117 + -1.0 2,258,117 + +0.7 2,258,118 - -0.4 2,258,118 - -1.0 2,258,118 - +1.0 2,258,118 - +0.1 2,258,118 - -0.2 2,258,179 - +0.2 2,258,575 + Pf1N1B4_1975 0.74 -0.4 2,258,576 - Pf1N1B4_1975 0.74 -1.8 2,258,576 - Pf1N1B4_1975 0.74 -1.2 2,258,576 - Pf1N1B4_1975 0.74 -0.0 2,258,695 + +1.5 2,258,695 + -0.0 2,258,695 + -1.0 2,258,821 + +1.5 2,258,821 + +0.8 2,258,821 + +1.5 2,258,821 + -0.1 2,258,821 + +0.5 2,258,821 + -0.8 2,258,821 + +0.7 2,258,821 + -2.4 2,258,822 - -1.3 2,258,822 - -0.8 2,258,822 - -1.4 2,258,822 - -1.3 2,258,822 - -0.0 2,258,822 - -1.2 2,258,822 - +1.1 2,258,822 - +0.7 2,259,024 + Pf1N1B4_1976 0.15 -1.5 2,259,024 + Pf1N1B4_1976 0.15 +0.5 2,259,024 + Pf1N1B4_1976 0.15 +1.5 2,259,024 + -0.5 2,259,025 - Pf1N1B4_1976 0.15 -0.8 2,259,025 - Pf1N1B4_1976 0.15 +0.5 2,259,025 - Pf1N1B4_1976 0.15 +0.9 2,259,025 - Pf1N1B4_1976 0.15 +1.5 2,259,025 - Pf1N1B4_1976 0.15 -1.6 2,259,025 - Pf1N1B4_1976 0.15 -1.5 2,259,098 + Pf1N1B4_1976 0.20 -0.5 2,259,098 + Pf1N1B4_1976 0.20 -1.4 2,259,100 + Pf1N1B4_1976 0.20 -1.6 2,259,100 + Pf1N1B4_1976 0.20 +0.1 2,259,100 + Pf1N1B4_1976 0.20 +0.2 2,259,100 + Pf1N1B4_1976 0.20 -1.6 2,259,101 - Pf1N1B4_1976 0.20 +0.2 2,259,101 - Pf1N1B4_1976 0.20 -1.0 2,259,101 - Pf1N1B4_1976 0.20 -0.6 2,259,166 + Pf1N1B4_1976 0.25 -0.6 2,259,166 + Pf1N1B4_1976 0.25 +0.2 2,259,166 + Pf1N1B4_1976 0.25 +1.5 2,259,166 + Pf1N1B4_1976 0.25 -0.0 2,259,167 - Pf1N1B4_1976 0.25 -1.8 2,259,190 + Pf1N1B4_1976 0.26 +0.4 2,259,190 + Pf1N1B4_1976 0.26 -0.3 2,259,190 + Pf1N1B4_1976 0.26 -0.6 2,259,190 + Pf1N1B4_1976 0.26 -0.3 2,259,191 - Pf1N1B4_1976 0.26 -0.6 2,259,191 - Pf1N1B4_1976 0.26 -0.3 2,259,191 - Pf1N1B4_1976 0.26 +2.5 2,259,191 - Pf1N1B4_1976 0.26 -0.2 2,259,191 - Pf1N1B4_1976 0.26 -0.5 2,259,191 - Pf1N1B4_1976 0.26 +0.2 2,259,389 + Pf1N1B4_1976 0.41 -1.6 2,259,389 + Pf1N1B4_1976 0.41 +0.2 2,259,390 - Pf1N1B4_1976 0.41 +0.5 2,259,390 - Pf1N1B4_1976 0.41 -0.5 2,259,390 - Pf1N1B4_1976 0.41 +1.6 2,259,390 - Pf1N1B4_1976 0.41 +0.9 2,259,391 + Pf1N1B4_1976 0.41 +1.7 2,259,391 + Pf1N1B4_1976 0.41 +0.7 2,259,391 + Pf1N1B4_1976 0.41 +0.1 2,259,391 + Pf1N1B4_1976 0.41 +0.3 2,259,391 + Pf1N1B4_1976 0.41 -1.6 2,259,392 - Pf1N1B4_1976 0.41 -0.9 2,259,421 + Pf1N1B4_1976 0.43 -0.2 2,259,422 - Pf1N1B4_1976 0.43 -1.0 2,259,422 - Pf1N1B4_1976 0.43 -0.5 2,259,422 - Pf1N1B4_1976 0.43 +2.5 2,260,042 + Pf1N1B4_1976 0.87 +0.7 2,260,042 + Pf1N1B4_1976 0.87 -1.1 2,260,042 + Pf1N1B4_1976 0.87 +0.8 2,260,043 - Pf1N1B4_1976 0.87 +0.1 2,260,043 - Pf1N1B4_1976 0.87 -1.6 2,260,043 - Pf1N1B4_1976 0.87 +0.3 2,260,172 + -0.7 2,260,173 - +0.5 2,260,173 - -2.1 2,260,173 - +0.6 2,260,210 + -0.4 2,260,210 + -1.6 2,260,210 + -0.7 2,260,210 + -1.0 2,260,210 + -0.3 2,260,210 + -1.9 2,260,210 + +1.0 2,260,210 + -0.9 2,260,210 + -0.0 2,260,210 + +0.2 2,260,210 + +2.1 2,260,210 + +1.3 2,260,210 + -0.5 2,260,210 + +1.0 2,260,211 - -0.3 2,260,211 - +0.8 2,260,211 - +1.5 2,260,211 - -0.8 2,260,211 - +0.3 2,260,211 - +1.7 2,260,211 - -0.5 2,260,229 + +0.4 2,260,229 + -1.0 2,260,230 - -0.8 2,260,230 - +1.1 2,260,230 - -0.5 2,260,230 - -0.5 2,260,263 + +0.2 2,260,263 + -0.6 2,260,263 + +0.2 2,260,263 + +1.3 2,260,263 + -0.5 2,260,264 - -0.3 2,260,264 - -1.8 2,260,280 + +1.5 2,260,281 - -1.8 2,260,288 + -1.6 2,260,288 + -1.0 2,260,288 + -0.5 2,260,288 + -1.8 2,260,288 + -0.8 2,260,289 - -0.0 2,260,289 - -0.3 2,260,289 - +0.2 2,260,289 - -0.0 2,260,291 + -0.5 2,260,350 + -1.6 2,260,350 + -1.0 2,260,350 + -1.5 2,260,350 + +0.0 2,260,350 + -0.1 2,260,350 + +0.5 2,260,351 - +1.5 2,260,351 - -0.3 2,260,351 - -0.5 2,260,351 - +1.5 2,260,354 + +0.0 2,260,354 + +1.0 2,260,354 + -2.5 2,260,354 + +0.2 2,260,355 - -0.3 2,260,355 - +1.3 2,260,364 + -0.8 2,260,373 + -1.0 2,260,373 + -0.3 2,260,373 + -0.0 2,260,373 + -0.0 2,260,373 + +0.7 2,260,373 + +1.5 2,260,373 + -1.0 2,260,374 - +3.0 2,260,374 - -0.5 2,260,374 - +0.8 2,260,374 - +0.8 2,260,374 - -1.6 2,260,374 - -0.7 2,260,432 - -0.0 2,260,466 + +3.1 2,260,466 + -0.6 2,260,466 + -0.5 2,260,466 + +0.7 2,260,466 + -0.2 2,260,466 + -1.3 2,260,466 + +0.5 2,260,466 + -0.8 2,260,466 + +0.8 2,260,466 + -1.2 2,260,466 + +1.1 2,260,466 + -1.6 2,260,466 + +0.2 2,260,466 + -2.2 2,260,466 + -0.8 2,260,466 + -1.0 2,260,466 + -0.3 2,260,467 - +1.3 2,260,467 - +1.2 2,260,467 - +1.0 2,260,467 - +0.2 2,260,467 - +2.5 2,260,467 - -0.6 2,260,467 - -0.0 2,260,467 - -0.9 2,260,475 + +0.7 2,260,762 + Pf1N1B4_1977 0.18 -0.5 2,260,762 + Pf1N1B4_1977 0.18 -1.0 2,260,832 + Pf1N1B4_1977 0.21 -0.7 2,260,864 + Pf1N1B4_1977 0.23 -1.2 2,260,864 + Pf1N1B4_1977 0.23 -1.0 2,260,864 + Pf1N1B4_1977 0.23 -0.3 2,260,865 - Pf1N1B4_1977 0.23 -0.6 2,260,865 - Pf1N1B4_1977 0.23 +0.7 2,261,084 + Pf1N1B4_1977 0.34 -0.2 2,261,084 + Pf1N1B4_1977 0.34 +1.1 2,261,084 + Pf1N1B4_1977 0.34 -1.9 2,261,085 - Pf1N1B4_1977 0.34 -0.5 2,261,085 - Pf1N1B4_1977 0.34 +0.8 2,261,085 - Pf1N1B4_1977 0.34 -0.5 2,261,085 - Pf1N1B4_1977 0.34 +2.1
Or see this region's nucleotide sequence