Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1724 and Pf1N1B4_1725 are separated by 3 nucleotides Pf1N1B4_1725 and Pf1N1B4_1726 are separated by 85 nucleotides
Pf1N1B4_1724 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 1,970,644 to 1,971,633
_1724
Pf1N1B4_1725 - Sulfate transport system permease protein CysW, at 1,971,637 to 1,972,434
_1725
Pf1N1B4_1726 - Sulfate transport system permease protein CysT, at 1,972,520 to 1,973,338
_1726
Position (kb)
1971
1972
1973 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1970.645 kb on + strand at 1970.645 kb on + strand at 1970.645 kb on + strand at 1970.646 kb on - strand at 1970.686 kb on + strand at 1970.687 kb on - strand at 1970.809 kb on - strand, within Pf1N1B4_1724 at 1970.809 kb on - strand, within Pf1N1B4_1724 at 1970.933 kb on + strand, within Pf1N1B4_1724 at 1971.015 kb on + strand, within Pf1N1B4_1724 at 1971.016 kb on - strand, within Pf1N1B4_1724 at 1971.386 kb on + strand, within Pf1N1B4_1724 at 1971.386 kb on + strand, within Pf1N1B4_1724 at 1971.387 kb on - strand, within Pf1N1B4_1724 at 1971.387 kb on - strand, within Pf1N1B4_1724 at 1971.387 kb on - strand, within Pf1N1B4_1724 at 1971.387 kb on - strand, within Pf1N1B4_1724 at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.673 kb on - strand at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.217 kb on + strand, within Pf1N1B4_1725 at 1972.218 kb on - strand, within Pf1N1B4_1725 at 1972.218 kb on - strand, within Pf1N1B4_1725 at 1972.491 kb on + strand at 1972.491 kb on + strand at 1972.492 kb on - strand at 1972.592 kb on + strand at 1972.592 kb on + strand at 1972.592 kb on + strand at 1972.593 kb on - strand at 1972.593 kb on - strand at 1972.617 kb on + strand, within Pf1N1B4_1726 at 1972.618 kb on - strand, within Pf1N1B4_1726 at 1972.623 kb on + strand, within Pf1N1B4_1726 at 1972.624 kb on - strand, within Pf1N1B4_1726 at 1972.797 kb on - strand, within Pf1N1B4_1726 at 1972.856 kb on + strand, within Pf1N1B4_1726 at 1972.870 kb on + strand, within Pf1N1B4_1726 at 1972.871 kb on - strand, within Pf1N1B4_1726 at 1972.871 kb on - strand at 1972.871 kb on - strand, within Pf1N1B4_1726 at 1972.874 kb on + strand, within Pf1N1B4_1726 at 1972.874 kb on + strand, within Pf1N1B4_1726 at 1972.875 kb on - strand, within Pf1N1B4_1726 at 1972.878 kb on - strand, within Pf1N1B4_1726 at 1972.878 kb on - strand, within Pf1N1B4_1726 at 1972.892 kb on + strand, within Pf1N1B4_1726 at 1972.892 kb on + strand, within Pf1N1B4_1726 at 1972.929 kb on + strand, within Pf1N1B4_1726 at 1972.929 kb on + strand, within Pf1N1B4_1726 at 1972.986 kb on + strand, within Pf1N1B4_1726 at 1972.986 kb on + strand, within Pf1N1B4_1726 at 1972.987 kb on - strand, within Pf1N1B4_1726 at 1973.079 kb on + strand, within Pf1N1B4_1726 at 1973.136 kb on + strand, within Pf1N1B4_1726 at 1973.137 kb on - strand, within Pf1N1B4_1726 at 1973.137 kb on - strand, within Pf1N1B4_1726 at 1973.216 kb on - strand, within Pf1N1B4_1726 at 1973.274 kb on + strand at 1973.274 kb on + strand at 1973.274 kb on + strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.286 kb on + strand at 1973.326 kb on + strand at 1973.327 kb on - strand at 1973.327 kb on - strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 1,970,645 + -1.7 1,970,645 + +0.2 1,970,645 + +0.2 1,970,646 - -2.0 1,970,686 + -2.0 1,970,687 - -0.0 1,970,809 - Pf1N1B4_1724 0.17 -1.8 1,970,809 - Pf1N1B4_1724 0.17 +0.4 1,970,933 + Pf1N1B4_1724 0.29 +1.0 1,971,015 + Pf1N1B4_1724 0.37 -0.0 1,971,016 - Pf1N1B4_1724 0.38 -0.8 1,971,386 + Pf1N1B4_1724 0.75 -1.4 1,971,386 + Pf1N1B4_1724 0.75 -0.3 1,971,387 - Pf1N1B4_1724 0.75 -1.5 1,971,387 - Pf1N1B4_1724 0.75 -0.0 1,971,387 - Pf1N1B4_1724 0.75 +0.2 1,971,387 - Pf1N1B4_1724 0.75 +0.5 1,971,638 + -0.8 1,971,638 + +1.3 1,971,638 + -2.3 1,971,638 + +0.5 1,971,638 + +0.5 1,971,638 + -1.9 1,971,639 - -0.5 1,971,639 - -0.0 1,971,639 - -1.6 1,971,639 - +0.4 1,971,639 - +0.1 1,971,673 - -1.0 1,971,761 + Pf1N1B4_1725 0.16 -2.0 1,971,761 + Pf1N1B4_1725 0.16 +0.3 1,971,761 + Pf1N1B4_1725 0.16 -1.8 1,971,762 - Pf1N1B4_1725 0.16 -0.2 1,971,762 - Pf1N1B4_1725 0.16 -1.9 1,971,762 - Pf1N1B4_1725 0.16 -0.5 1,971,770 + Pf1N1B4_1725 0.17 -1.5 1,971,770 + Pf1N1B4_1725 0.17 -1.3 1,971,770 + Pf1N1B4_1725 0.17 -2.9 1,971,770 + Pf1N1B4_1725 0.17 -0.6 1,971,771 - Pf1N1B4_1725 0.17 -1.3 1,971,771 - Pf1N1B4_1725 0.17 -0.7 1,971,771 - Pf1N1B4_1725 0.17 -1.0 1,971,771 - Pf1N1B4_1725 0.17 -0.5 1,971,771 - Pf1N1B4_1725 0.17 -0.5 1,971,884 + Pf1N1B4_1725 0.31 +0.5 1,971,884 + Pf1N1B4_1725 0.31 -2.1 1,971,884 + Pf1N1B4_1725 0.31 +0.5 1,971,884 + Pf1N1B4_1725 0.31 +2.5 1,971,884 + Pf1N1B4_1725 0.31 -1.6 1,971,885 - Pf1N1B4_1725 0.31 -1.0 1,971,885 - Pf1N1B4_1725 0.31 -1.3 1,971,885 - Pf1N1B4_1725 0.31 -2.4 1,972,132 + Pf1N1B4_1725 0.62 -1.0 1,972,132 + Pf1N1B4_1725 0.62 -1.3 1,972,132 + Pf1N1B4_1725 0.62 -0.0 1,972,132 + Pf1N1B4_1725 0.62 +0.1 1,972,133 - Pf1N1B4_1725 0.62 -1.2 1,972,133 - Pf1N1B4_1725 0.62 -1.2 1,972,133 - Pf1N1B4_1725 0.62 -0.9 1,972,133 - Pf1N1B4_1725 0.62 +0.2 1,972,133 - Pf1N1B4_1725 0.62 -1.3 1,972,133 - Pf1N1B4_1725 0.62 -3.0 1,972,133 - Pf1N1B4_1725 0.62 -1.3 1,972,133 - Pf1N1B4_1725 0.62 -1.8 1,972,133 - Pf1N1B4_1725 0.62 -0.8 1,972,133 - +0.5 1,972,133 - Pf1N1B4_1725 0.62 -2.2 1,972,133 - Pf1N1B4_1725 0.62 -0.0 1,972,133 - Pf1N1B4_1725 0.62 -0.0 1,972,133 - Pf1N1B4_1725 0.62 -0.8 1,972,133 - Pf1N1B4_1725 0.62 -1.5 1,972,133 - Pf1N1B4_1725 0.62 -1.6 1,972,133 - Pf1N1B4_1725 0.62 -1.6 1,972,133 - Pf1N1B4_1725 0.62 -0.5 1,972,133 - Pf1N1B4_1725 0.62 -2.6 1,972,136 + Pf1N1B4_1725 0.63 -0.8 1,972,136 + Pf1N1B4_1725 0.63 -0.7 1,972,136 + Pf1N1B4_1725 0.63 -0.5 1,972,136 + Pf1N1B4_1725 0.63 -0.8 1,972,136 + Pf1N1B4_1725 0.63 -1.4 1,972,136 + Pf1N1B4_1725 0.63 -0.8 1,972,136 + Pf1N1B4_1725 0.63 -1.3 1,972,136 + Pf1N1B4_1725 0.63 -1.5 1,972,136 + Pf1N1B4_1725 0.63 -0.0 1,972,137 - Pf1N1B4_1725 0.63 +0.4 1,972,137 - Pf1N1B4_1725 0.63 -2.4 1,972,137 - Pf1N1B4_1725 0.63 -0.5 1,972,137 - Pf1N1B4_1725 0.63 -3.0 1,972,137 - Pf1N1B4_1725 0.63 +1.1 1,972,137 - Pf1N1B4_1725 0.63 -2.0 1,972,137 - Pf1N1B4_1725 0.63 +0.5 1,972,137 - Pf1N1B4_1725 0.63 -1.6 1,972,137 - Pf1N1B4_1725 0.63 -0.8 1,972,137 - Pf1N1B4_1725 0.63 -1.0 1,972,217 + Pf1N1B4_1725 0.73 -2.0 1,972,218 - Pf1N1B4_1725 0.73 -0.5 1,972,218 - Pf1N1B4_1725 0.73 -1.9 1,972,491 + -1.9 1,972,491 + -0.8 1,972,492 - +0.1 1,972,592 + -2.6 1,972,592 + -0.6 1,972,592 + -1.6 1,972,593 - -1.5 1,972,593 - -0.3 1,972,617 + Pf1N1B4_1726 0.12 +0.8 1,972,618 - Pf1N1B4_1726 0.12 -0.3 1,972,623 + Pf1N1B4_1726 0.13 -1.3 1,972,624 - Pf1N1B4_1726 0.13 -2.2 1,972,797 - Pf1N1B4_1726 0.34 -2.4 1,972,856 + Pf1N1B4_1726 0.41 -1.5 1,972,870 + Pf1N1B4_1726 0.43 +0.5 1,972,871 - Pf1N1B4_1726 0.43 -0.1 1,972,871 - +0.5 1,972,871 - Pf1N1B4_1726 0.43 -1.5 1,972,874 + Pf1N1B4_1726 0.43 -0.5 1,972,874 + Pf1N1B4_1726 0.43 -0.5 1,972,875 - Pf1N1B4_1726 0.43 +0.3 1,972,878 - Pf1N1B4_1726 0.44 -0.2 1,972,878 - Pf1N1B4_1726 0.44 -0.1 1,972,892 + Pf1N1B4_1726 0.45 -1.9 1,972,892 + Pf1N1B4_1726 0.45 -1.6 1,972,929 + Pf1N1B4_1726 0.50 -0.5 1,972,929 + Pf1N1B4_1726 0.50 -1.3 1,972,986 + Pf1N1B4_1726 0.57 -1.3 1,972,986 + Pf1N1B4_1726 0.57 -1.6 1,972,987 - Pf1N1B4_1726 0.57 -0.1 1,973,079 + Pf1N1B4_1726 0.68 -1.1 1,973,136 + Pf1N1B4_1726 0.75 -1.4 1,973,137 - Pf1N1B4_1726 0.75 -2.1 1,973,137 - Pf1N1B4_1726 0.75 -0.4 1,973,216 - Pf1N1B4_1726 0.85 -1.3 1,973,274 + -1.9 1,973,274 + +0.5 1,973,274 + -0.6 1,973,275 - -0.8 1,973,275 - +0.5 1,973,275 - -4.2 1,973,275 - -1.3 1,973,275 - -0.1 1,973,275 - -2.4 1,973,275 - +0.3 1,973,286 + -1.1 1,973,326 + +0.9 1,973,327 - -1.1 1,973,327 - +1.5 1,973,338 + -1.5 1,973,338 + +0.2 1,973,338 + -1.1 1,973,338 + -1.1 1,973,338 + -2.6 1,973,338 + -1.1 1,973,338 + -2.2 1,973,338 + -0.5 1,973,338 + +1.1 1,973,338 + -0.5 1,973,338 + +0.5 1,973,338 + -1.1 1,973,410 + -0.3 1,973,410 + -0.3 1,973,410 + -0.4 1,973,410 + -2.1 1,973,410 + -0.9 1,973,410 + -0.8 1,973,410 + -0.1 1,973,410 + -0.8 1,973,410 + -0.5 1,973,410 + -1.3 1,973,410 + -1.5 1,973,410 + +0.2 1,973,410 + +0.4 1,973,410 + -1.1 1,973,411 - +0.8 1,973,411 - -1.1 1,973,411 - +2.5 1,973,411 - -0.2 1,973,411 - +1.5 1,973,411 - +2.3 1,973,411 - +0.8 1,973,411 - +1.5 1,973,418 - +0.9
Or see this region's nucleotide sequence