Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_13 and Pf1N1B4_14 are separated by 152 nucleotides Pf1N1B4_14 and Pf1N1B4_15 are separated by 11 nucleotides Pf1N1B4_15 and Pf1N1B4_16 are separated by 158 nucleotides
Pf1N1B4_13 - SAM-dependent methyltransferase YafE (UbiE paralog), at 14,449 to 15,216
_13
Pf1N1B4_14 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 15,369 to 16,013
_14
Pf1N1B4_15 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 16,025 to 17,443
_15
Pf1N1B4_16 - Cys regulon transcriptional activator CysB, at 17,602 to 18,492
_16
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 15.152 kb on + strand at 15.152 kb on + strand at 15.184 kb on + strand at 15.184 kb on + strand at 15.184 kb on + strand at 15.185 kb on - strand at 15.187 kb on + strand at 15.679 kb on + strand, within Pf1N1B4_14 at 15.680 kb on - strand, within Pf1N1B4_14 at 15.680 kb on - strand, within Pf1N1B4_14 at 16.026 kb on + strand at 16.027 kb on - strand at 16.455 kb on + strand, within Pf1N1B4_15 at 16.455 kb on + strand, within Pf1N1B4_15 at 16.456 kb on - strand, within Pf1N1B4_15 at 16.456 kb on - strand, within Pf1N1B4_15 at 16.456 kb on - strand, within Pf1N1B4_15 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.774 kb on + strand, within Pf1N1B4_15 at 16.775 kb on - strand, within Pf1N1B4_15 at 17.165 kb on - strand, within Pf1N1B4_15 at 17.364 kb on + strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.471 kb on - strand at 17.823 kb on - strand, within Pf1N1B4_16 at 17.927 kb on + strand, within Pf1N1B4_16 at 17.931 kb on - strand, within Pf1N1B4_16 at 18.271 kb on + strand, within Pf1N1B4_16 at 18.272 kb on - strand at 18.392 kb on + strand, within Pf1N1B4_16 at 18.392 kb on + strand, within Pf1N1B4_16 at 18.392 kb on + strand, within Pf1N1B4_16 at 18.398 kb on - strand at 18.398 kb on - strand, within Pf1N1B4_16
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 15,152 + -1.0 15,152 + -1.7 15,184 + +0.6 15,184 + -0.4 15,184 + -1.7 15,185 - +0.6 15,187 + -0.2 15,679 + Pf1N1B4_14 0.48 -1.6 15,680 - Pf1N1B4_14 0.48 -1.4 15,680 - Pf1N1B4_14 0.48 -2.1 16,026 + -2.3 16,027 - +0.3 16,455 + Pf1N1B4_15 0.30 -0.8 16,455 + Pf1N1B4_15 0.30 -1.0 16,456 - Pf1N1B4_15 0.30 -2.9 16,456 - Pf1N1B4_15 0.30 -2.0 16,456 - Pf1N1B4_15 0.30 -2.1 16,704 + Pf1N1B4_15 0.48 -1.6 16,704 + Pf1N1B4_15 0.48 -2.3 16,704 + Pf1N1B4_15 0.48 -0.7 16,774 + Pf1N1B4_15 0.53 -1.5 16,775 - Pf1N1B4_15 0.53 +1.0 17,165 - Pf1N1B4_15 0.80 -4.9 17,364 + +1.6 17,365 - -0.7 17,365 - -2.9 17,365 - -1.2 17,365 - -2.3 17,365 - -1.2 17,471 - +0.4 17,823 - Pf1N1B4_16 0.25 -0.4 17,927 + Pf1N1B4_16 0.36 +1.4 17,931 - Pf1N1B4_16 0.37 +0.9 18,271 + Pf1N1B4_16 0.75 +1.4 18,272 - +1.0 18,392 + Pf1N1B4_16 0.89 -2.5 18,392 + Pf1N1B4_16 0.89 -2.4 18,392 + Pf1N1B4_16 0.89 -1.2 18,398 - +0.6 18,398 - Pf1N1B4_16 0.89 -0.4
Or see this region's nucleotide sequence