Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1183 and Pf1N1B4_1184 are separated by 229 nucleotides Pf1N1B4_1184 and Pf1N1B4_1185 are separated by 142 nucleotides Pf1N1B4_1185 and Pf1N1B4_1186 are separated by 262 nucleotides
Pf1N1B4_1183 - Sodium-dependent transporter family protein, at 1,333,337 to 1,334,629
_1183
Pf1N1B4_1184 - tmRNA-binding protein SmpB, at 1,334,859 to 1,335,341
_1184
Pf1N1B4_1185 - Lactate-responsive regulator LldR in Enterobacteria, GntR family, at 1,335,484 to 1,336,251
_1185
Pf1N1B4_1186 - L-lactate permease, at 1,336,514 to 1,338,205
_1186
Position (kb)
1335
1336
1337 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1334.579 kb on - strand at 1334.616 kb on + strand at 1334.617 kb on - strand at 1334.617 kb on - strand at 1334.631 kb on + strand at 1334.631 kb on + strand at 1334.631 kb on + strand at 1334.631 kb on + strand at 1334.631 kb on + strand at 1334.631 kb on + strand at 1334.631 kb on + strand at 1334.631 kb on + strand at 1334.632 kb on - strand at 1334.632 kb on - strand at 1334.632 kb on - strand at 1334.647 kb on + strand at 1334.647 kb on + strand at 1334.647 kb on + strand at 1334.647 kb on + strand at 1334.647 kb on + strand at 1334.647 kb on + strand at 1334.647 kb on + strand at 1334.647 kb on + strand at 1334.648 kb on - strand at 1334.648 kb on - strand at 1334.684 kb on + strand at 1334.844 kb on + strand at 1335.339 kb on + strand at 1335.339 kb on + strand at 1335.340 kb on - strand at 1335.340 kb on - strand at 1335.388 kb on + strand at 1335.388 kb on + strand at 1335.388 kb on + strand at 1335.389 kb on - strand at 1335.389 kb on - strand at 1335.408 kb on + strand at 1335.408 kb on + strand at 1335.408 kb on + strand at 1335.409 kb on - strand at 1335.409 kb on - strand at 1335.409 kb on - strand at 1335.409 kb on - strand at 1335.477 kb on + strand at 1335.478 kb on - strand at 1335.478 kb on - strand at 1335.485 kb on + strand at 1335.485 kb on + strand at 1335.485 kb on + strand at 1335.485 kb on + strand at 1335.485 kb on + strand at 1335.485 kb on + strand at 1335.486 kb on - strand at 1335.486 kb on - strand at 1335.486 kb on - strand at 1335.486 kb on - strand at 1335.486 kb on - strand at 1335.486 kb on - strand at 1335.486 kb on - strand at 1335.486 kb on - strand at 1335.486 kb on - strand at 1335.486 kb on - strand at 1335.560 kb on + strand at 1335.560 kb on + strand at 1335.560 kb on + strand at 1335.560 kb on + strand at 1335.561 kb on - strand, within Pf1N1B4_1185 at 1335.561 kb on - strand, within Pf1N1B4_1185 at 1335.561 kb on - strand, within Pf1N1B4_1185 at 1335.561 kb on - strand, within Pf1N1B4_1185 at 1335.561 kb on - strand, within Pf1N1B4_1185 at 1335.623 kb on + strand at 1335.624 kb on - strand, within Pf1N1B4_1185 at 1335.624 kb on - strand, within Pf1N1B4_1185 at 1335.624 kb on - strand, within Pf1N1B4_1185 at 1335.624 kb on - strand, within Pf1N1B4_1185 at 1335.677 kb on + strand, within Pf1N1B4_1185 at 1335.677 kb on + strand at 1335.677 kb on + strand, within Pf1N1B4_1185 at 1335.678 kb on - strand, within Pf1N1B4_1185 at 1335.678 kb on - strand, within Pf1N1B4_1185 at 1335.678 kb on - strand at 1335.678 kb on - strand, within Pf1N1B4_1185 at 1335.678 kb on - strand at 1335.678 kb on - strand, within Pf1N1B4_1185 at 1335.840 kb on - strand, within Pf1N1B4_1185 at 1335.911 kb on + strand, within Pf1N1B4_1185 at 1335.911 kb on + strand, within Pf1N1B4_1185 at 1335.914 kb on + strand at 1335.915 kb on - strand, within Pf1N1B4_1185 at 1335.915 kb on - strand, within Pf1N1B4_1185 at 1335.915 kb on - strand, within Pf1N1B4_1185 at 1335.915 kb on - strand, within Pf1N1B4_1185 at 1335.936 kb on + strand, within Pf1N1B4_1185 at 1335.937 kb on - strand, within Pf1N1B4_1185 at 1336.034 kb on + strand, within Pf1N1B4_1185 at 1336.034 kb on + strand, within Pf1N1B4_1185 at 1336.034 kb on + strand, within Pf1N1B4_1185 at 1336.035 kb on - strand, within Pf1N1B4_1185 at 1336.035 kb on - strand, within Pf1N1B4_1185 at 1336.035 kb on - strand, within Pf1N1B4_1185 at 1336.035 kb on - strand, within Pf1N1B4_1185 at 1336.035 kb on - strand, within Pf1N1B4_1185 at 1336.035 kb on - strand, within Pf1N1B4_1185 at 1336.035 kb on - strand, within Pf1N1B4_1185 at 1336.210 kb on - strand at 1336.292 kb on + strand at 1336.293 kb on - strand at 1336.293 kb on - strand at 1336.293 kb on - strand at 1336.303 kb on - strand at 1336.411 kb on + strand at 1336.411 kb on + strand at 1336.411 kb on + strand at 1336.412 kb on - strand at 1336.423 kb on - strand at 1336.452 kb on - strand at 1336.455 kb on - strand at 1336.462 kb on - strand at 1336.499 kb on + strand at 1336.499 kb on + strand at 1336.500 kb on - strand at 1336.500 kb on - strand at 1336.500 kb on - strand at 1336.500 kb on - strand at 1336.500 kb on - strand at 1336.500 kb on - strand at 1336.513 kb on + strand at 1336.535 kb on + strand at 1336.590 kb on + strand at 1336.590 kb on + strand at 1336.648 kb on + strand at 1336.649 kb on - strand at 1336.736 kb on + strand, within Pf1N1B4_1186 at 1336.737 kb on - strand, within Pf1N1B4_1186 at 1336.784 kb on + strand, within Pf1N1B4_1186 at 1336.976 kb on + strand, within Pf1N1B4_1186 at 1337.199 kb on + strand, within Pf1N1B4_1186 at 1337.199 kb on + strand, within Pf1N1B4_1186 at 1337.200 kb on - strand, within Pf1N1B4_1186 at 1337.208 kb on + strand, within Pf1N1B4_1186 at 1337.209 kb on - strand, within Pf1N1B4_1186 at 1337.209 kb on - strand, within Pf1N1B4_1186 at 1337.228 kb on + strand, within Pf1N1B4_1186 at 1337.228 kb on + strand, within Pf1N1B4_1186 at 1337.228 kb on + strand, within Pf1N1B4_1186 at 1337.229 kb on - strand, within Pf1N1B4_1186 at 1337.229 kb on - strand, within Pf1N1B4_1186 at 1337.229 kb on - strand, within Pf1N1B4_1186
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 1,334,579 - -0.1 1,334,616 + -0.5 1,334,617 - +0.3 1,334,617 - +1.5 1,334,631 + -0.5 1,334,631 + +0.9 1,334,631 + +0.5 1,334,631 + -0.5 1,334,631 + +0.5 1,334,631 + -1.6 1,334,631 + +1.5 1,334,631 + +0.8 1,334,632 - -1.6 1,334,632 - -0.3 1,334,632 - +1.5 1,334,647 + +1.5 1,334,647 + -1.9 1,334,647 + -0.8 1,334,647 + -1.1 1,334,647 + +0.8 1,334,647 + -0.1 1,334,647 + +0.8 1,334,647 + +0.1 1,334,648 - -0.6 1,334,648 - -0.3 1,334,684 + +1.9 1,334,844 + +0.5 1,335,339 + +1.1 1,335,339 + +1.5 1,335,340 - +2.1 1,335,340 - +0.2 1,335,388 + +0.9 1,335,388 + -0.8 1,335,388 + +0.1 1,335,389 - -0.8 1,335,389 - -0.1 1,335,408 + +1.2 1,335,408 + -1.1 1,335,408 + +0.9 1,335,409 - +0.5 1,335,409 - +0.1 1,335,409 - +0.5 1,335,409 - +0.1 1,335,477 + +0.2 1,335,478 - +0.3 1,335,478 - -1.4 1,335,485 + +0.2 1,335,485 + +0.4 1,335,485 + -0.1 1,335,485 + -1.6 1,335,485 + +1.5 1,335,485 + +0.8 1,335,486 - -2.2 1,335,486 - +0.9 1,335,486 - -0.7 1,335,486 - -0.4 1,335,486 - -0.1 1,335,486 - +1.4 1,335,486 - -1.8 1,335,486 - +0.0 1,335,486 - +0.7 1,335,486 - -0.2 1,335,560 + +2.3 1,335,560 + +5.7 1,335,560 + +2.5 1,335,560 + +2.9 1,335,561 - Pf1N1B4_1185 0.10 +2.3 1,335,561 - Pf1N1B4_1185 0.10 +3.4 1,335,561 - Pf1N1B4_1185 0.10 +4.4 1,335,561 - Pf1N1B4_1185 0.10 +5.9 1,335,561 - Pf1N1B4_1185 0.10 +4.5 1,335,623 + +1.5 1,335,624 - Pf1N1B4_1185 0.18 +4.1 1,335,624 - Pf1N1B4_1185 0.18 +4.0 1,335,624 - Pf1N1B4_1185 0.18 +3.5 1,335,624 - Pf1N1B4_1185 0.18 +3.8 1,335,677 + Pf1N1B4_1185 0.25 +2.9 1,335,677 + +0.3 1,335,677 + Pf1N1B4_1185 0.25 +2.2 1,335,678 - Pf1N1B4_1185 0.25 +3.8 1,335,678 - Pf1N1B4_1185 0.25 +1.5 1,335,678 - +1.5 1,335,678 - Pf1N1B4_1185 0.25 +2.4 1,335,678 - +2.5 1,335,678 - Pf1N1B4_1185 0.25 +3.9 1,335,840 - Pf1N1B4_1185 0.46 +3.0 1,335,911 + Pf1N1B4_1185 0.56 +4.4 1,335,911 + Pf1N1B4_1185 0.56 +2.6 1,335,914 + +3.5 1,335,915 - Pf1N1B4_1185 0.56 +5.5 1,335,915 - Pf1N1B4_1185 0.56 +3.2 1,335,915 - Pf1N1B4_1185 0.56 +3.5 1,335,915 - Pf1N1B4_1185 0.56 +4.1 1,335,936 + Pf1N1B4_1185 0.59 +4.3 1,335,937 - Pf1N1B4_1185 0.59 +4.1 1,336,034 + Pf1N1B4_1185 0.72 +2.1 1,336,034 + Pf1N1B4_1185 0.72 +1.8 1,336,034 + Pf1N1B4_1185 0.72 +3.1 1,336,035 - Pf1N1B4_1185 0.72 +2.4 1,336,035 - Pf1N1B4_1185 0.72 +6.0 1,336,035 - Pf1N1B4_1185 0.72 +3.2 1,336,035 - Pf1N1B4_1185 0.72 +3.1 1,336,035 - Pf1N1B4_1185 0.72 +3.8 1,336,035 - Pf1N1B4_1185 0.72 +4.4 1,336,035 - Pf1N1B4_1185 0.72 +3.0 1,336,210 - +5.8 1,336,292 + +0.9 1,336,293 - -0.5 1,336,293 - -0.8 1,336,293 - -2.3 1,336,303 - -0.5 1,336,411 + +2.4 1,336,411 + +0.9 1,336,411 + +0.1 1,336,412 - -0.9 1,336,423 - +0.5 1,336,452 - -3.3 1,336,455 - -0.2 1,336,462 - +1.2 1,336,499 + -0.8 1,336,499 + +0.8 1,336,500 - -1.5 1,336,500 - -0.8 1,336,500 - +0.2 1,336,500 - -0.8 1,336,500 - -0.9 1,336,500 - -1.6 1,336,513 + -1.6 1,336,535 + -1.1 1,336,590 + -1.4 1,336,590 + -0.1 1,336,648 + -0.6 1,336,649 - -0.6 1,336,736 + Pf1N1B4_1186 0.13 +0.3 1,336,737 - Pf1N1B4_1186 0.13 -0.1 1,336,784 + Pf1N1B4_1186 0.16 -1.9 1,336,976 + Pf1N1B4_1186 0.27 +0.2 1,337,199 + Pf1N1B4_1186 0.40 +0.5 1,337,199 + Pf1N1B4_1186 0.40 +0.2 1,337,200 - Pf1N1B4_1186 0.41 -1.8 1,337,208 + Pf1N1B4_1186 0.41 -0.5 1,337,209 - Pf1N1B4_1186 0.41 -1.3 1,337,209 - Pf1N1B4_1186 0.41 -1.9 1,337,228 + Pf1N1B4_1186 0.42 -0.8 1,337,228 + Pf1N1B4_1186 0.42 -0.7 1,337,228 + Pf1N1B4_1186 0.42 -0.4 1,337,229 - Pf1N1B4_1186 0.42 -0.1 1,337,229 - Pf1N1B4_1186 0.42 +0.5 1,337,229 - Pf1N1B4_1186 0.42 +0.5
Or see this region's nucleotide sequence