Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_764

Experiment: Nitrate reduction, replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_763 and Pf1N1B4_764 are separated by 115 nucleotidesPf1N1B4_764 and Pf1N1B4_765 are separated by 116 nucleotides Pf1N1B4_763 - Ferric iron ABC transporter, ATP-binding protein, at 867,093 to 868,202 _763 Pf1N1B4_764 - Uncharacterized isomerase yddE, PhzC-PhzF family, at 868,318 to 869,100 _764 Pf1N1B4_765 - Cys-tRNA(Pro) deacylase YbaK, at 869,217 to 869,687 _765 Position (kb) 868 869 870Strain fitness (log2 ratio) -2 -1 0 1 2 3at 867.628 kb on + strand, within Pf1N1B4_763at 867.628 kb on + strand, within Pf1N1B4_763at 867.629 kb on - strandat 867.629 kb on - strand, within Pf1N1B4_763at 867.629 kb on - strand, within Pf1N1B4_763at 867.757 kb on - strand, within Pf1N1B4_763at 867.765 kb on + strand, within Pf1N1B4_763at 867.814 kb on - strand, within Pf1N1B4_763at 867.832 kb on + strand, within Pf1N1B4_763at 867.833 kb on - strand, within Pf1N1B4_763at 867.872 kb on - strand, within Pf1N1B4_763at 867.872 kb on - strand, within Pf1N1B4_763at 867.888 kb on + strand, within Pf1N1B4_763at 867.888 kb on + strand, within Pf1N1B4_763at 867.889 kb on - strand, within Pf1N1B4_763at 867.889 kb on - strand, within Pf1N1B4_763at 867.889 kb on - strand, within Pf1N1B4_763at 868.090 kb on + strand, within Pf1N1B4_763at 868.249 kb on - strandat 868.274 kb on + strandat 868.275 kb on - strandat 868.275 kb on - strandat 868.275 kb on - strandat 868.330 kb on + strandat 868.331 kb on - strandat 868.331 kb on - strandat 868.331 kb on - strandat 868.331 kb on - strandat 868.427 kb on + strand, within Pf1N1B4_764at 868.428 kb on - strand, within Pf1N1B4_764at 868.654 kb on + strand, within Pf1N1B4_764at 868.655 kb on - strand, within Pf1N1B4_764at 868.844 kb on + strand, within Pf1N1B4_764at 868.844 kb on + strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.862 kb on + strand, within Pf1N1B4_764at 868.863 kb on - strand, within Pf1N1B4_764at 868.863 kb on - strand, within Pf1N1B4_764at 868.863 kb on - strand, within Pf1N1B4_764at 868.914 kb on + strand, within Pf1N1B4_764at 869.092 kb on + strandat 869.093 kb on - strandat 869.093 kb on - strandat 869.093 kb on - strandat 869.183 kb on + strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.355 kb on - strand, within Pf1N1B4_765at 869.355 kb on - strand, within Pf1N1B4_765at 869.602 kb on + strand, within Pf1N1B4_765at 869.602 kb on + strand, within Pf1N1B4_765at 869.602 kb on + strand, within Pf1N1B4_765at 869.602 kb on + strandat 869.629 kb on + strand, within Pf1N1B4_765at 869.629 kb on + strand, within Pf1N1B4_765at 869.736 kb on - strandat 869.751 kb on - strandat 869.763 kb on + strandat 869.763 kb on + strandat 869.763 kb on + strandat 869.763 kb on + strandat 869.763 kb on + strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.771 kb on + strandat 869.772 kb on - strandat 869.855 kb on + strandat 869.936 kb on + strandat 869.936 kb on + strandat 869.936 kb on + strandat 870.073 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B
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867,628 + Pf1N1B4_763 0.48 +0.4
867,628 + Pf1N1B4_763 0.48 -1.4
867,629 - +1.0
867,629 - Pf1N1B4_763 0.48 +0.7
867,629 - Pf1N1B4_763 0.48 -0.2
867,757 - Pf1N1B4_763 0.60 +0.4
867,765 + Pf1N1B4_763 0.61 +2.1
867,814 - Pf1N1B4_763 0.65 -0.2
867,832 + Pf1N1B4_763 0.67 -1.5
867,833 - Pf1N1B4_763 0.67 +1.4
867,872 - Pf1N1B4_763 0.70 -0.7
867,872 - Pf1N1B4_763 0.70 +0.9
867,888 + Pf1N1B4_763 0.72 +0.4
867,888 + Pf1N1B4_763 0.72 +0.4
867,889 - Pf1N1B4_763 0.72 -0.9
867,889 - Pf1N1B4_763 0.72 +1.4
867,889 - Pf1N1B4_763 0.72 +1.0
868,090 + Pf1N1B4_763 0.90 +0.1
868,249 - +1.4
868,274 + +0.1
868,275 - -0.7
868,275 - -1.1
868,275 - -1.2
868,330 + -0.6
868,331 - +0.1
868,331 - -0.6
868,331 - +0.2
868,331 - +0.3
868,427 + Pf1N1B4_764 0.14 +0.5
868,428 - Pf1N1B4_764 0.14 +1.4
868,654 + Pf1N1B4_764 0.43 -1.5
868,655 - Pf1N1B4_764 0.43 +1.2
868,844 + Pf1N1B4_764 0.67 -1.2
868,844 + Pf1N1B4_764 0.67 +0.2
868,845 - Pf1N1B4_764 0.67 -0.2
868,845 - Pf1N1B4_764 0.67 -2.4
868,845 - Pf1N1B4_764 0.67 +0.4
868,845 - Pf1N1B4_764 0.67 +0.7
868,845 - Pf1N1B4_764 0.67 +0.8
868,845 - Pf1N1B4_764 0.67 +3.4
868,862 + Pf1N1B4_764 0.69 +0.3
868,863 - Pf1N1B4_764 0.70 -0.6
868,863 - Pf1N1B4_764 0.70 +0.1
868,863 - Pf1N1B4_764 0.70 +0.2
868,914 + Pf1N1B4_764 0.76 -0.2
869,092 + -2.2
869,093 - -1.4
869,093 - +0.7
869,093 - +0.8
869,183 + -1.2
869,184 - +1.9
869,184 - -0.2
869,184 - -0.2
869,184 - -1.4
869,184 - -0.3
869,184 - -0.2
869,355 - Pf1N1B4_765 0.29 +1.4
869,355 - Pf1N1B4_765 0.29 -0.2
869,602 + Pf1N1B4_765 0.82 +0.7
869,602 + Pf1N1B4_765 0.82 +0.8
869,602 + Pf1N1B4_765 0.82 -0.6
869,602 + +1.4
869,629 + Pf1N1B4_765 0.87 +0.1
869,629 + Pf1N1B4_765 0.87 +0.1
869,736 - +0.7
869,751 - -1.6
869,763 + -1.9
869,763 + -0.2
869,763 + -0.4
869,763 + +3.0
869,763 + -0.6
869,764 - +1.4
869,764 - -0.0
869,764 - +2.4
869,764 - +2.6
869,764 - -2.2
869,764 - -1.0
869,764 - -0.9
869,771 + -0.9
869,772 - -1.7
869,855 + +2.1
869,936 + -1.6
869,936 + -0.8
869,936 + -0.2
870,073 - +2.0

Or see this region's nucleotide sequence