Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_733 and Pf1N1B4_734 are separated by 129 nucleotides Pf1N1B4_734 and Pf1N1B4_735 overlap by 4 nucleotides
Pf1N1B4_733 - Methionyl-tRNA synthetase (EC 6.1.1.10), at 835,415 to 837,466
_733
Pf1N1B4_734 - Electron transport complex protein RnfA, at 837,596 to 838,165
_734
Pf1N1B4_735 - Electron transport complex protein RnfB, at 838,162 to 839,169
_735
Pf1N1B4_736 - Electron transport complex protein RnfD, at 839,156 to 840,148
_736
Position (kb) 837
838
839 Strain fitness (log2 ratio) -2
-1
0
1
2 at 837.030 kb on + strand, within Pf1N1B4_733 at 837.030 kb on + strand, within Pf1N1B4_733 at 837.030 kb on + strand, within Pf1N1B4_733 at 837.465 kb on - strand at 837.465 kb on - strand at 837.558 kb on + strand at 837.758 kb on + strand, within Pf1N1B4_734 at 837.768 kb on - strand, within Pf1N1B4_734 at 837.777 kb on + strand, within Pf1N1B4_734 at 837.778 kb on - strand, within Pf1N1B4_734 at 837.778 kb on - strand, within Pf1N1B4_734 at 837.780 kb on + strand, within Pf1N1B4_734 at 837.780 kb on + strand, within Pf1N1B4_734 at 837.781 kb on - strand, within Pf1N1B4_734 at 837.984 kb on + strand, within Pf1N1B4_734 at 838.233 kb on + strand at 838.233 kb on + strand at 838.233 kb on + strand at 838.233 kb on + strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.241 kb on - strand at 838.241 kb on - strand at 838.384 kb on + strand, within Pf1N1B4_735 at 838.385 kb on - strand, within Pf1N1B4_735 at 838.385 kb on - strand, within Pf1N1B4_735 at 839.155 kb on + strand at 839.156 kb on - strand at 839.156 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 837,030 + Pf1N1B4_733 0.79 +0.2 837,030 + Pf1N1B4_733 0.79 +2.0 837,030 + Pf1N1B4_733 0.79 -1.0 837,465 - +1.6 837,465 - +0.1 837,558 + -1.3 837,758 + Pf1N1B4_734 0.28 -0.1 837,768 - Pf1N1B4_734 0.30 +0.9 837,777 + Pf1N1B4_734 0.32 -0.1 837,778 - Pf1N1B4_734 0.32 -0.6 837,778 - Pf1N1B4_734 0.32 -1.4 837,780 + Pf1N1B4_734 0.32 -0.9 837,780 + Pf1N1B4_734 0.32 +0.9 837,781 - Pf1N1B4_734 0.32 -2.3 837,984 + Pf1N1B4_734 0.68 -0.9 838,233 + -1.4 838,233 + +1.9 838,233 + -0.9 838,233 + +1.2 838,234 - +1.4 838,234 - +1.4 838,234 - +0.4 838,234 - -1.6 838,234 - -0.9 838,234 - +1.0 838,234 - -1.3 838,234 - +2.0 838,241 - -0.9 838,241 - +0.4 838,384 + Pf1N1B4_735 0.22 +0.4 838,385 - Pf1N1B4_735 0.22 +0.7 838,385 - Pf1N1B4_735 0.22 -0.1 839,155 + +1.0 839,156 - -0.6 839,156 - -0.9
Or see this region's nucleotide sequence