Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_728 and Pf1N1B4_729 are separated by 66 nucleotides Pf1N1B4_729 and Pf1N1B4_730 are separated by 169 nucleotides Pf1N1B4_730 and Pf1N1B4_731 are separated by 130 nucleotides
Pf1N1B4_728 - FxsA protein, at 831,620 to 832,096
_728
Pf1N1B4_729 - Putative heme iron utilization protein, at 832,163 to 832,894
_729
Pf1N1B4_730 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 833,064 to 833,822
_730
Pf1N1B4_731 - Scaffold protein for [4Fe-4S] cluster assembly ApbC, MRP-like, at 833,953 to 835,047
_731
Position (kb)
833
834 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 832.070 kb on + strand at 832.071 kb on - strand at 832.139 kb on - strand at 832.139 kb on - strand at 832.139 kb on - strand at 832.246 kb on + strand, within Pf1N1B4_729 at 832.246 kb on + strand, within Pf1N1B4_729 at 832.361 kb on + strand, within Pf1N1B4_729 at 832.361 kb on + strand, within Pf1N1B4_729 at 832.361 kb on + strand, within Pf1N1B4_729 at 832.361 kb on + strand, within Pf1N1B4_729 at 832.361 kb on + strand, within Pf1N1B4_729 at 832.361 kb on + strand, within Pf1N1B4_729 at 832.361 kb on + strand, within Pf1N1B4_729 at 832.361 kb on + strand, within Pf1N1B4_729 at 832.361 kb on + strand, within Pf1N1B4_729 at 832.362 kb on - strand, within Pf1N1B4_729 at 832.362 kb on - strand, within Pf1N1B4_729 at 832.365 kb on + strand, within Pf1N1B4_729 at 832.365 kb on + strand, within Pf1N1B4_729 at 832.366 kb on - strand, within Pf1N1B4_729 at 832.491 kb on + strand, within Pf1N1B4_729 at 832.492 kb on - strand, within Pf1N1B4_729 at 832.492 kb on - strand, within Pf1N1B4_729 at 832.502 kb on + strand, within Pf1N1B4_729 at 832.502 kb on + strand, within Pf1N1B4_729 at 832.503 kb on - strand, within Pf1N1B4_729 at 832.503 kb on - strand, within Pf1N1B4_729 at 832.545 kb on + strand at 832.545 kb on + strand, within Pf1N1B4_729 at 832.545 kb on + strand, within Pf1N1B4_729 at 832.575 kb on + strand, within Pf1N1B4_729 at 832.576 kb on - strand, within Pf1N1B4_729 at 832.635 kb on + strand, within Pf1N1B4_729 at 832.635 kb on + strand, within Pf1N1B4_729 at 832.636 kb on - strand, within Pf1N1B4_729 at 832.636 kb on - strand, within Pf1N1B4_729 at 832.658 kb on + strand, within Pf1N1B4_729 at 832.658 kb on + strand, within Pf1N1B4_729 at 832.658 kb on + strand, within Pf1N1B4_729 at 832.658 kb on + strand, within Pf1N1B4_729 at 832.658 kb on + strand, within Pf1N1B4_729 at 832.658 kb on + strand, within Pf1N1B4_729 at 832.658 kb on + strand, within Pf1N1B4_729 at 832.658 kb on + strand at 832.658 kb on + strand, within Pf1N1B4_729 at 832.659 kb on - strand, within Pf1N1B4_729 at 832.659 kb on - strand, within Pf1N1B4_729 at 832.659 kb on - strand, within Pf1N1B4_729 at 832.742 kb on + strand, within Pf1N1B4_729 at 832.742 kb on + strand, within Pf1N1B4_729 at 832.742 kb on + strand, within Pf1N1B4_729 at 832.742 kb on + strand, within Pf1N1B4_729 at 832.742 kb on + strand, within Pf1N1B4_729 at 832.742 kb on + strand, within Pf1N1B4_729 at 832.742 kb on + strand, within Pf1N1B4_729 at 832.742 kb on + strand, within Pf1N1B4_729 at 832.743 kb on - strand, within Pf1N1B4_729 at 832.743 kb on - strand at 832.744 kb on + strand, within Pf1N1B4_729 at 832.744 kb on + strand, within Pf1N1B4_729 at 832.744 kb on + strand, within Pf1N1B4_729 at 832.779 kb on + strand, within Pf1N1B4_729 at 832.884 kb on - strand at 832.897 kb on + strand at 832.907 kb on + strand at 832.907 kb on + strand at 832.907 kb on + strand at 832.907 kb on + strand at 832.907 kb on + strand at 832.907 kb on + strand at 832.908 kb on - strand at 832.908 kb on - strand at 832.910 kb on - strand at 832.910 kb on - strand at 832.911 kb on + strand at 832.911 kb on + strand at 832.911 kb on + strand at 832.911 kb on + strand at 832.911 kb on + strand at 832.912 kb on - strand at 832.912 kb on - strand at 832.993 kb on + strand at 833.019 kb on + strand at 833.020 kb on - strand at 833.020 kb on - strand at 833.024 kb on + strand at 833.024 kb on + strand at 833.024 kb on + strand at 833.024 kb on + strand at 833.025 kb on - strand at 833.025 kb on - strand at 833.025 kb on - strand at 833.025 kb on - strand at 833.025 kb on - strand at 833.025 kb on - strand at 833.049 kb on + strand at 833.083 kb on + strand at 833.084 kb on - strand at 833.113 kb on + strand at 833.113 kb on + strand at 833.133 kb on + strand at 833.133 kb on + strand at 833.238 kb on + strand, within Pf1N1B4_730 at 833.239 kb on - strand at 833.276 kb on + strand, within Pf1N1B4_730 at 833.276 kb on + strand at 833.276 kb on + strand, within Pf1N1B4_730 at 833.504 kb on + strand, within Pf1N1B4_730 at 833.504 kb on + strand, within Pf1N1B4_730 at 833.504 kb on + strand, within Pf1N1B4_730 at 833.504 kb on + strand, within Pf1N1B4_730 at 833.504 kb on + strand, within Pf1N1B4_730 at 833.504 kb on + strand, within Pf1N1B4_730 at 833.505 kb on - strand at 833.547 kb on + strand, within Pf1N1B4_730 at 833.547 kb on + strand, within Pf1N1B4_730 at 833.547 kb on + strand, within Pf1N1B4_730 at 833.547 kb on + strand, within Pf1N1B4_730 at 833.548 kb on - strand, within Pf1N1B4_730 at 833.548 kb on - strand, within Pf1N1B4_730 at 833.548 kb on - strand, within Pf1N1B4_730 at 833.757 kb on + strand at 833.758 kb on - strand at 833.758 kb on - strand at 833.775 kb on + strand at 833.775 kb on + strand at 833.775 kb on + strand at 833.775 kb on + strand at 833.775 kb on + strand at 833.776 kb on - strand at 833.776 kb on - strand at 833.776 kb on - strand at 833.776 kb on - strand at 833.778 kb on + strand at 833.778 kb on + strand at 833.778 kb on + strand at 833.778 kb on + strand at 833.778 kb on + strand at 833.778 kb on + strand at 833.778 kb on + strand at 833.779 kb on - strand at 833.779 kb on - strand at 833.820 kb on + strand at 833.820 kb on + strand at 833.821 kb on - strand at 833.883 kb on + strand at 833.883 kb on + strand at 833.883 kb on + strand at 833.884 kb on - strand at 833.884 kb on - strand at 833.897 kb on + strand at 833.898 kb on - strand at 833.898 kb on - strand at 833.899 kb on + strand at 833.899 kb on + strand at 833.899 kb on + strand at 833.900 kb on - strand at 833.954 kb on + strand at 833.955 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 832,070 + +0.7 832,071 - +0.9 832,139 - +0.4 832,139 - +2.0 832,139 - -1.6 832,246 + Pf1N1B4_729 0.11 -1.2 832,246 + Pf1N1B4_729 0.11 +0.4 832,361 + Pf1N1B4_729 0.27 +0.3 832,361 + Pf1N1B4_729 0.27 +0.9 832,361 + Pf1N1B4_729 0.27 -0.2 832,361 + Pf1N1B4_729 0.27 -1.4 832,361 + Pf1N1B4_729 0.27 +0.3 832,361 + Pf1N1B4_729 0.27 +1.0 832,361 + Pf1N1B4_729 0.27 +1.8 832,361 + Pf1N1B4_729 0.27 +0.7 832,361 + Pf1N1B4_729 0.27 +0.4 832,362 - Pf1N1B4_729 0.27 +0.9 832,362 - Pf1N1B4_729 0.27 -1.7 832,365 + Pf1N1B4_729 0.28 +0.4 832,365 + Pf1N1B4_729 0.28 -1.4 832,366 - Pf1N1B4_729 0.28 +0.4 832,491 + Pf1N1B4_729 0.45 +0.4 832,492 - Pf1N1B4_729 0.45 +0.3 832,492 - Pf1N1B4_729 0.45 -1.7 832,502 + Pf1N1B4_729 0.46 -0.8 832,502 + Pf1N1B4_729 0.46 +0.7 832,503 - Pf1N1B4_729 0.46 +0.4 832,503 - Pf1N1B4_729 0.46 -0.7 832,545 + -0.6 832,545 + Pf1N1B4_729 0.52 +0.8 832,545 + Pf1N1B4_729 0.52 -0.7 832,575 + Pf1N1B4_729 0.56 +0.3 832,576 - Pf1N1B4_729 0.56 +0.5 832,635 + Pf1N1B4_729 0.64 -1.6 832,635 + Pf1N1B4_729 0.64 -0.3 832,636 - Pf1N1B4_729 0.65 -0.8 832,636 - Pf1N1B4_729 0.65 +1.5 832,658 + Pf1N1B4_729 0.68 -0.9 832,658 + Pf1N1B4_729 0.68 +0.1 832,658 + Pf1N1B4_729 0.68 -2.1 832,658 + Pf1N1B4_729 0.68 +0.2 832,658 + Pf1N1B4_729 0.68 +0.4 832,658 + Pf1N1B4_729 0.68 +0.2 832,658 + Pf1N1B4_729 0.68 +0.4 832,658 + +2.2 832,658 + Pf1N1B4_729 0.68 +2.0 832,659 - Pf1N1B4_729 0.68 +0.2 832,659 - Pf1N1B4_729 0.68 +1.1 832,659 - Pf1N1B4_729 0.68 +0.1 832,742 + Pf1N1B4_729 0.79 +0.8 832,742 + Pf1N1B4_729 0.79 -0.6 832,742 + Pf1N1B4_729 0.79 -0.6 832,742 + Pf1N1B4_729 0.79 -1.4 832,742 + Pf1N1B4_729 0.79 +0.9 832,742 + Pf1N1B4_729 0.79 -0.1 832,742 + Pf1N1B4_729 0.79 -0.4 832,742 + Pf1N1B4_729 0.79 +1.2 832,743 - Pf1N1B4_729 0.79 -2.1 832,743 - -0.6 832,744 + Pf1N1B4_729 0.79 -0.3 832,744 + Pf1N1B4_729 0.79 -0.1 832,744 + Pf1N1B4_729 0.79 +0.4 832,779 + Pf1N1B4_729 0.84 +0.2 832,884 - +0.4 832,897 + +1.3 832,907 + +2.4 832,907 + -0.3 832,907 + +1.4 832,907 + -0.7 832,907 + +1.2 832,907 + +0.4 832,908 - +3.1 832,908 - -2.0 832,910 - +0.3 832,910 - -0.9 832,911 + +0.6 832,911 + -0.3 832,911 + -0.1 832,911 + +0.3 832,911 + -1.1 832,912 - +2.2 832,912 - -1.1 832,993 + +1.2 833,019 + +0.9 833,020 - -1.6 833,020 - -0.8 833,024 + -1.2 833,024 + +0.0 833,024 + +0.5 833,024 + -0.7 833,025 - -1.4 833,025 - -1.1 833,025 - -0.4 833,025 - +0.4 833,025 - -0.6 833,025 - -0.2 833,049 + -1.2 833,083 + -1.5 833,084 - -1.4 833,113 + +0.1 833,113 + -0.9 833,133 + -2.7 833,133 + +0.4 833,238 + Pf1N1B4_730 0.23 -0.4 833,239 - +1.4 833,276 + Pf1N1B4_730 0.28 +0.2 833,276 + -0.6 833,276 + Pf1N1B4_730 0.28 +0.9 833,504 + Pf1N1B4_730 0.58 +0.4 833,504 + Pf1N1B4_730 0.58 -0.2 833,504 + Pf1N1B4_730 0.58 -0.3 833,504 + Pf1N1B4_730 0.58 -1.3 833,504 + Pf1N1B4_730 0.58 -0.9 833,504 + Pf1N1B4_730 0.58 +0.8 833,505 - +0.1 833,547 + Pf1N1B4_730 0.64 -0.7 833,547 + Pf1N1B4_730 0.64 -0.9 833,547 + Pf1N1B4_730 0.64 +0.1 833,547 + Pf1N1B4_730 0.64 -1.7 833,548 - Pf1N1B4_730 0.64 -1.1 833,548 - Pf1N1B4_730 0.64 -0.4 833,548 - Pf1N1B4_730 0.64 -0.0 833,757 + -0.7 833,758 - +0.1 833,758 - -1.7 833,775 + -0.7 833,775 + +0.2 833,775 + -2.7 833,775 + -0.2 833,775 + -0.2 833,776 - -0.6 833,776 - +2.0 833,776 - +0.9 833,776 - +0.1 833,778 + -0.2 833,778 + +0.4 833,778 + +0.7 833,778 + -1.0 833,778 + -1.0 833,778 + +0.3 833,778 + -0.3 833,779 - +0.8 833,779 - -0.9 833,820 + +0.1 833,820 + -0.3 833,821 - -0.7 833,883 + +0.2 833,883 + +0.7 833,883 + +0.2 833,884 - -0.6 833,884 - -2.2 833,897 + +1.0 833,898 - -1.0 833,898 - -0.7 833,899 + -0.4 833,899 + +0.3 833,899 + +0.3 833,900 - +1.8 833,954 + -2.2 833,955 - -1.2
Or see this region's nucleotide sequence