Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4 and Pf1N1B4_5 are separated by 183 nucleotides Pf1N1B4_5 and Pf1N1B4_6 are separated by 41 nucleotides Pf1N1B4_6 and Pf1N1B4_7 are separated by 63 nucleotides
Pf1N1B4_4 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 3,678 to 4,598
_4
Pf1N1B4_5 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 4,782 to 4,922
_5
Pf1N1B4_6 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 4,964 to 5,401
_6
Pf1N1B4_7 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 5,465 to 6,319
_7
Position (kb) 4
5
6 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4.673 kb on - strand at 4.673 kb on - strand at 4.673 kb on - strand at 4.673 kb on - strand at 4.673 kb on - strand at 4.673 kb on - strand at 4.724 kb on - strand at 4.724 kb on - strand at 4.724 kb on - strand at 4.736 kb on - strand at 4.736 kb on - strand at 4.852 kb on - strand, within Pf1N1B4_5 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.014 kb on - strand, within Pf1N1B4_6 at 5.103 kb on + strand, within Pf1N1B4_6 at 5.104 kb on - strand at 5.104 kb on - strand, within Pf1N1B4_6 at 5.104 kb on - strand, within Pf1N1B4_6 at 5.104 kb on - strand, within Pf1N1B4_6 at 5.104 kb on - strand, within Pf1N1B4_6 at 5.304 kb on + strand, within Pf1N1B4_6 at 5.305 kb on - strand, within Pf1N1B4_6 at 5.361 kb on - strand at 5.361 kb on - strand at 5.361 kb on - strand at 5.512 kb on - strand at 5.512 kb on - strand at 5.552 kb on + strand, within Pf1N1B4_7 at 5.560 kb on - strand, within Pf1N1B4_7 at 5.603 kb on - strand, within Pf1N1B4_7 at 5.753 kb on + strand at 5.753 kb on + strand, within Pf1N1B4_7 at 5.754 kb on - strand, within Pf1N1B4_7 at 5.754 kb on - strand, within Pf1N1B4_7 at 5.754 kb on - strand, within Pf1N1B4_7 at 5.887 kb on + strand, within Pf1N1B4_7 at 5.888 kb on - strand, within Pf1N1B4_7 at 5.928 kb on + strand, within Pf1N1B4_7 at 5.929 kb on - strand, within Pf1N1B4_7 at 5.929 kb on - strand, within Pf1N1B4_7 at 6.247 kb on - strand at 6.305 kb on - strand at 6.305 kb on - strand at 6.308 kb on + strand at 6.308 kb on + strand at 6.308 kb on + strand at 6.309 kb on - strand at 6.309 kb on - strand at 6.309 kb on - strand at 6.309 kb on - strand at 6.310 kb on + strand at 6.311 kb on - strand at 6.357 kb on + strand at 6.357 kb on + strand at 6.357 kb on + strand at 6.358 kb on - strand at 6.358 kb on - strand at 6.358 kb on - strand at 6.358 kb on - strand at 6.359 kb on + strand at 6.359 kb on + strand at 6.359 kb on + strand at 6.359 kb on + strand at 6.359 kb on + strand at 6.360 kb on - strand at 6.360 kb on - strand at 6.360 kb on - strand at 6.360 kb on - strand at 6.360 kb on - strand at 6.360 kb on - strand at 6.360 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 4,673 - -0.3 4,673 - -1.0 4,673 - -0.5 4,673 - +0.0 4,673 - +0.3 4,673 - -0.4 4,724 - +0.0 4,724 - -0.6 4,724 - -1.8 4,736 - +0.2 4,736 - +0.3 4,852 - Pf1N1B4_5 0.50 -1.3 5,014 - Pf1N1B4_6 0.11 -4.0 5,014 - Pf1N1B4_6 0.11 -4.7 5,014 - Pf1N1B4_6 0.11 -2.3 5,014 - Pf1N1B4_6 0.11 -3.7 5,014 - Pf1N1B4_6 0.11 -2.3 5,014 - Pf1N1B4_6 0.11 -0.8 5,014 - Pf1N1B4_6 0.11 -1.1 5,014 - Pf1N1B4_6 0.11 -2.4 5,103 + Pf1N1B4_6 0.32 -2.3 5,104 - -1.1 5,104 - Pf1N1B4_6 0.32 +0.5 5,104 - Pf1N1B4_6 0.32 -1.7 5,104 - Pf1N1B4_6 0.32 -3.2 5,104 - Pf1N1B4_6 0.32 -3.0 5,304 + Pf1N1B4_6 0.78 -3.9 5,305 - Pf1N1B4_6 0.78 -1.3 5,361 - -2.6 5,361 - -0.2 5,361 - -2.8 5,512 - -0.4 5,512 - -1.3 5,552 + Pf1N1B4_7 0.10 +1.5 5,560 - Pf1N1B4_7 0.11 -1.3 5,603 - Pf1N1B4_7 0.16 -0.3 5,753 + -0.5 5,753 + Pf1N1B4_7 0.34 +0.7 5,754 - Pf1N1B4_7 0.34 -1.3 5,754 - Pf1N1B4_7 0.34 +0.6 5,754 - Pf1N1B4_7 0.34 +2.4 5,887 + Pf1N1B4_7 0.49 +0.6 5,888 - Pf1N1B4_7 0.49 -0.5 5,928 + Pf1N1B4_7 0.54 +1.2 5,929 - Pf1N1B4_7 0.54 +0.1 5,929 - Pf1N1B4_7 0.54 -0.1 6,247 - +0.1 6,305 - -1.1 6,305 - +0.8 6,308 + +1.2 6,308 + +1.7 6,308 + -0.1 6,309 - -0.5 6,309 - -0.6 6,309 - +0.1 6,309 - -0.4 6,310 + -0.1 6,311 - -0.2 6,357 + -3.7 6,357 + -0.9 6,357 + -0.5 6,358 - +0.7 6,358 - -1.0 6,358 - -0.0 6,358 - -0.5 6,359 + -0.1 6,359 + +0.5 6,359 + -0.1 6,359 + +1.9 6,359 + +0.2 6,360 - +0.3 6,360 - +0.5 6,360 - +0.9 6,360 - +0.9 6,360 - -0.2 6,360 - +0.8 6,360 - -1.0
Or see this region's nucleotide sequence