Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5498 and Pf1N1B4_5499 are separated by 367 nucleotides Pf1N1B4_5499 and Pf1N1B4_5500 are separated by 177 nucleotides
Pf1N1B4_5498 - hypothetical protein, at 247,310 to 247,522
_5498
Pf1N1B4_5499 - hypothetical protein, at 247,890 to 249,695
_5499
Pf1N1B4_5500 - hypothetical protein, at 249,873 to 250,862
_5500
Position (kb) 247
248
249
250 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 247.038 kb on + strand at 247.038 kb on + strand at 247.041 kb on - strand at 247.041 kb on - strand at 247.109 kb on + strand at 247.150 kb on - strand at 247.191 kb on + strand at 247.192 kb on - strand at 247.219 kb on + strand at 247.252 kb on + strand at 247.252 kb on + strand at 247.253 kb on - strand at 247.253 kb on - strand at 247.255 kb on - strand at 247.361 kb on + strand, within Pf1N1B4_5498 at 247.362 kb on - strand, within Pf1N1B4_5498 at 247.362 kb on - strand, within Pf1N1B4_5498 at 247.462 kb on + strand, within Pf1N1B4_5498 at 247.462 kb on + strand, within Pf1N1B4_5498 at 247.631 kb on + strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.683 kb on + strand at 247.684 kb on - strand at 247.705 kb on + strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.780 kb on + strand at 247.780 kb on + strand at 247.780 kb on + strand at 247.781 kb on - strand at 247.781 kb on - strand at 247.855 kb on + strand at 248.092 kb on + strand, within Pf1N1B4_5499 at 248.092 kb on + strand, within Pf1N1B4_5499 at 248.093 kb on - strand at 248.320 kb on + strand, within Pf1N1B4_5499 at 248.356 kb on + strand at 248.356 kb on + strand, within Pf1N1B4_5499 at 248.356 kb on + strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.435 kb on - strand, within Pf1N1B4_5499 at 248.435 kb on - strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.516 kb on - strand, within Pf1N1B4_5499 at 248.516 kb on - strand, within Pf1N1B4_5499 at 248.577 kb on + strand at 248.577 kb on + strand, within Pf1N1B4_5499 at 248.578 kb on - strand, within Pf1N1B4_5499 at 248.578 kb on - strand, within Pf1N1B4_5499 at 248.578 kb on - strand, within Pf1N1B4_5499 at 248.645 kb on - strand at 248.654 kb on + strand, within Pf1N1B4_5499 at 248.654 kb on + strand, within Pf1N1B4_5499 at 248.654 kb on + strand, within Pf1N1B4_5499 at 248.654 kb on + strand, within Pf1N1B4_5499 at 248.654 kb on + strand, within Pf1N1B4_5499 at 248.655 kb on - strand at 248.655 kb on - strand, within Pf1N1B4_5499 at 248.655 kb on - strand, within Pf1N1B4_5499 at 248.785 kb on + strand, within Pf1N1B4_5499 at 248.786 kb on - strand, within Pf1N1B4_5499 at 248.796 kb on + strand at 248.900 kb on + strand, within Pf1N1B4_5499 at 248.971 kb on + strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 249.004 kb on - strand at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.071 kb on - strand, within Pf1N1B4_5499 at 249.232 kb on + strand, within Pf1N1B4_5499 at 249.232 kb on + strand at 249.232 kb on + strand, within Pf1N1B4_5499 at 249.233 kb on - strand, within Pf1N1B4_5499 at 249.233 kb on - strand at 249.246 kb on - strand, within Pf1N1B4_5499 at 249.246 kb on - strand, within Pf1N1B4_5499 at 249.274 kb on + strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.560 kb on + strand at 249.571 kb on + strand at 249.571 kb on + strand at 249.571 kb on + strand at 249.572 kb on - strand at 249.572 kb on - strand at 249.623 kb on + strand at 249.627 kb on + strand at 249.627 kb on + strand at 249.627 kb on + strand at 249.628 kb on - strand at 249.682 kb on + strand at 249.683 kb on - strand at 249.683 kb on - strand at 249.742 kb on - strand at 249.840 kb on - strand at 249.840 kb on - strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.882 kb on + strand at 249.883 kb on - strand at 249.883 kb on - strand at 249.883 kb on - strand at 250.144 kb on + strand, within Pf1N1B4_5500 at 250.145 kb on - strand, within Pf1N1B4_5500 at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.268 kb on - strand, within Pf1N1B4_5500 at 250.365 kb on + strand, within Pf1N1B4_5500 at 250.365 kb on + strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 247,038 + +0.5 247,038 + -2.1 247,041 - -0.1 247,041 - -0.3 247,109 + +0.1 247,150 - +1.5 247,191 + +1.4 247,192 - +0.5 247,219 + -0.3 247,252 + -1.6 247,252 + -0.4 247,253 - +0.4 247,253 - +1.4 247,255 - -1.5 247,361 + Pf1N1B4_5498 0.24 -2.2 247,362 - Pf1N1B4_5498 0.24 -1.4 247,362 - Pf1N1B4_5498 0.24 -1.2 247,462 + Pf1N1B4_5498 0.71 -0.2 247,462 + Pf1N1B4_5498 0.71 +0.1 247,631 + -1.3 247,632 - -2.8 247,632 - +0.2 247,632 - +0.3 247,632 - +0.1 247,683 + -0.6 247,684 - -0.1 247,705 + -0.8 247,706 - +1.2 247,706 - +1.7 247,706 - -0.2 247,706 - -0.6 247,780 + -1.4 247,780 + +0.7 247,780 + -0.2 247,781 - -0.4 247,781 - +0.7 247,855 + -2.9 248,092 + Pf1N1B4_5499 0.11 -0.6 248,092 + Pf1N1B4_5499 0.11 +0.6 248,093 - +2.5 248,320 + Pf1N1B4_5499 0.24 -0.4 248,356 + -0.2 248,356 + Pf1N1B4_5499 0.26 -1.4 248,356 + Pf1N1B4_5499 0.26 +0.6 248,357 - Pf1N1B4_5499 0.26 +0.6 248,357 - Pf1N1B4_5499 0.26 +1.2 248,357 - Pf1N1B4_5499 0.26 -1.0 248,357 - Pf1N1B4_5499 0.26 -0.9 248,357 - +1.8 248,434 + Pf1N1B4_5499 0.30 -0.2 248,434 + Pf1N1B4_5499 0.30 -1.6 248,434 + Pf1N1B4_5499 0.30 +0.6 248,434 + Pf1N1B4_5499 0.30 -0.6 248,434 + Pf1N1B4_5499 0.30 -0.6 248,434 + Pf1N1B4_5499 0.30 -0.2 248,435 - Pf1N1B4_5499 0.30 +0.4 248,435 - Pf1N1B4_5499 0.30 +0.1 248,515 + Pf1N1B4_5499 0.35 +0.4 248,515 + Pf1N1B4_5499 0.35 -0.3 248,515 + Pf1N1B4_5499 0.35 -0.2 248,516 - Pf1N1B4_5499 0.35 -0.6 248,516 - Pf1N1B4_5499 0.35 -0.1 248,577 + +2.7 248,577 + Pf1N1B4_5499 0.38 -0.4 248,578 - Pf1N1B4_5499 0.38 -0.8 248,578 - Pf1N1B4_5499 0.38 +2.4 248,578 - Pf1N1B4_5499 0.38 -0.2 248,645 - +1.4 248,654 + Pf1N1B4_5499 0.42 -1.4 248,654 + Pf1N1B4_5499 0.42 +3.1 248,654 + Pf1N1B4_5499 0.42 -0.6 248,654 + Pf1N1B4_5499 0.42 +0.9 248,654 + Pf1N1B4_5499 0.42 +0.3 248,655 - -0.6 248,655 - Pf1N1B4_5499 0.42 -0.5 248,655 - Pf1N1B4_5499 0.42 +0.1 248,785 + Pf1N1B4_5499 0.50 -1.0 248,786 - Pf1N1B4_5499 0.50 +0.9 248,796 + -1.2 248,900 + Pf1N1B4_5499 0.56 -0.4 248,971 + Pf1N1B4_5499 0.60 -1.3 248,972 - Pf1N1B4_5499 0.60 +0.2 248,972 - Pf1N1B4_5499 0.60 -0.1 248,972 - Pf1N1B4_5499 0.60 -0.4 249,004 - +0.0 249,070 + Pf1N1B4_5499 0.65 +0.4 249,070 + Pf1N1B4_5499 0.65 +1.7 249,070 + Pf1N1B4_5499 0.65 +0.9 249,071 - Pf1N1B4_5499 0.65 -0.6 249,232 + Pf1N1B4_5499 0.74 +0.1 249,232 + -0.4 249,232 + Pf1N1B4_5499 0.74 -0.2 249,233 - Pf1N1B4_5499 0.74 +0.2 249,233 - -0.2 249,246 - Pf1N1B4_5499 0.75 +0.4 249,246 - Pf1N1B4_5499 0.75 -0.4 249,274 + Pf1N1B4_5499 0.77 +0.9 249,450 - Pf1N1B4_5499 0.86 -2.2 249,450 - Pf1N1B4_5499 0.86 +0.1 249,450 - Pf1N1B4_5499 0.86 -0.2 249,560 + +2.8 249,571 + -0.4 249,571 + -0.8 249,571 + +0.0 249,572 - +0.7 249,572 - +1.2 249,623 + -0.8 249,627 + +1.4 249,627 + -1.0 249,627 + -1.1 249,628 - +0.4 249,682 + +1.0 249,683 - -1.0 249,683 - -2.4 249,742 - -0.3 249,840 - +0.9 249,840 - +0.1 249,880 + -0.9 249,880 + +0.1 249,880 + -0.2 249,880 + +0.1 249,880 + -0.1 249,880 + -1.4 249,881 - +0.7 249,881 - -0.4 249,881 - -0.1 249,881 - +0.3 249,881 - +0.1 249,881 - -0.3 249,881 - -1.2 249,881 - +0.1 249,881 - +0.5 249,882 + +0.1 249,883 - +0.4 249,883 - +1.2 249,883 - -2.1 250,144 + Pf1N1B4_5500 0.27 +0.5 250,145 - Pf1N1B4_5500 0.27 +1.9 250,224 + Pf1N1B4_5500 0.35 -1.4 250,224 + -0.6 250,224 + Pf1N1B4_5500 0.35 -1.2 250,224 + -1.8 250,224 + Pf1N1B4_5500 0.35 -1.0 250,224 + Pf1N1B4_5500 0.35 -0.4 250,225 - Pf1N1B4_5500 0.36 +0.1 250,225 - Pf1N1B4_5500 0.36 -1.0 250,225 - Pf1N1B4_5500 0.36 -1.6 250,225 - Pf1N1B4_5500 0.36 +1.4 250,225 - Pf1N1B4_5500 0.36 -0.2 250,225 - Pf1N1B4_5500 0.36 +1.4 250,225 - Pf1N1B4_5500 0.36 -0.3 250,268 - Pf1N1B4_5500 0.40 +2.4 250,365 + Pf1N1B4_5500 0.50 +1.4 250,365 + Pf1N1B4_5500 0.50 -0.5 250,366 - Pf1N1B4_5500 0.50 +0.3 250,366 - Pf1N1B4_5500 0.50 -1.5 250,366 - Pf1N1B4_5500 0.50 +0.8 250,366 - Pf1N1B4_5500 0.50 +0.4 250,429 + Pf1N1B4_5500 0.56 +0.8 250,429 + Pf1N1B4_5500 0.56 +0.4 250,430 - Pf1N1B4_5500 0.56 -1.9 250,430 - Pf1N1B4_5500 0.56 -0.4 250,430 - Pf1N1B4_5500 0.56 -0.2 250,430 - Pf1N1B4_5500 0.56 -0.1 250,689 - Pf1N1B4_5500 0.82 +0.0 250,689 - Pf1N1B4_5500 0.82 -0.5
Or see this region's nucleotide sequence