Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4045

Experiment: Nitrate reduction, replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4043 and Pf1N1B4_4044 are separated by 236 nucleotidesPf1N1B4_4044 and Pf1N1B4_4045 overlap by 8 nucleotidesPf1N1B4_4045 and Pf1N1B4_4046 are separated by 86 nucleotides Pf1N1B4_4043 - Type II and III secretion system family protein, at 4,495,666 to 4,496,427 _4043 Pf1N1B4_4044 - GNAT family acetyltransferase YjcF, at 4,496,664 to 4,497,089 _4044 Pf1N1B4_4045 - FIG002776: hypothetical protein, at 4,497,082 to 4,498,251 _4045 Pf1N1B4_4046 - Adenylosuccinate lyase (EC 4.3.2.2), at 4,498,338 to 4,499,708 _4046 Position (kb) 4497 4498 4499Strain fitness (log2 ratio) -1 0 1 2at 4496.154 kb on + strand, within Pf1N1B4_4043at 4496.155 kb on - strand, within Pf1N1B4_4043at 4496.428 kb on - strandat 4496.657 kb on - strandat 4496.750 kb on - strand, within Pf1N1B4_4044at 4496.750 kb on - strand, within Pf1N1B4_4044at 4496.750 kb on - strand, within Pf1N1B4_4044at 4497.084 kb on - strandat 4497.839 kb on + strand, within Pf1N1B4_4045

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B
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4,496,154 + Pf1N1B4_4043 0.64 -0.3
4,496,155 - Pf1N1B4_4043 0.64 +0.5
4,496,428 - +0.5
4,496,657 - +1.3
4,496,750 - Pf1N1B4_4044 0.20 +0.6
4,496,750 - Pf1N1B4_4044 0.20 +1.8
4,496,750 - Pf1N1B4_4044 0.20 +0.6
4,497,084 - -0.9
4,497,839 + Pf1N1B4_4045 0.65 +0.8

Or see this region's nucleotide sequence