Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4001 and Pf1N1B4_4002 are separated by 201 nucleotides Pf1N1B4_4002 and Pf1N1B4_4003 are separated by 126 nucleotides Pf1N1B4_4003 and Pf1N1B4_4004 overlap by 4 nucleotides
Pf1N1B4_4001 - Membrane fusion component of tripartite multidrug resistance system, at 4,449,471 to 4,450,673
_4001
Pf1N1B4_4002 - Inner membrane component of tripartite multidrug resistance system, at 4,450,875 to 4,452,404
_4002
Pf1N1B4_4003 - UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54), at 4,452,531 to 4,453,283
_4003
Pf1N1B4_4004 - Peptidyl-prolyl cis-trans isomerase PpiB (EC 5.2.1.8), at 4,453,280 to 4,453,783
_4004
Position (kb)
4450
4451
4452
4453 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4450.329 kb on + strand, within Pf1N1B4_4001 at 4450.329 kb on + strand, within Pf1N1B4_4001 at 4450.330 kb on - strand, within Pf1N1B4_4001 at 4450.584 kb on + strand at 4450.584 kb on + strand at 4450.584 kb on + strand at 4450.584 kb on + strand at 4450.585 kb on - strand at 4450.585 kb on - strand at 4450.585 kb on - strand at 4450.585 kb on - strand at 4450.585 kb on - strand at 4450.585 kb on - strand at 4451.233 kb on - strand, within Pf1N1B4_4002 at 4451.367 kb on + strand, within Pf1N1B4_4002 at 4451.367 kb on + strand, within Pf1N1B4_4002 at 4451.368 kb on - strand at 4451.368 kb on - strand, within Pf1N1B4_4002 at 4451.481 kb on + strand, within Pf1N1B4_4002 at 4451.481 kb on + strand, within Pf1N1B4_4002 at 4451.482 kb on - strand, within Pf1N1B4_4002 at 4451.853 kb on + strand at 4451.854 kb on - strand, within Pf1N1B4_4002 at 4452.162 kb on + strand, within Pf1N1B4_4002 at 4452.162 kb on + strand, within Pf1N1B4_4002 at 4452.162 kb on + strand, within Pf1N1B4_4002 at 4452.162 kb on + strand, within Pf1N1B4_4002 at 4452.162 kb on + strand, within Pf1N1B4_4002 at 4452.162 kb on + strand, within Pf1N1B4_4002 at 4452.163 kb on - strand, within Pf1N1B4_4002 at 4452.163 kb on - strand, within Pf1N1B4_4002 at 4452.163 kb on - strand, within Pf1N1B4_4002 at 4452.163 kb on - strand, within Pf1N1B4_4002 at 4452.179 kb on + strand, within Pf1N1B4_4002 at 4452.186 kb on + strand, within Pf1N1B4_4002 at 4452.186 kb on + strand, within Pf1N1B4_4002 at 4452.279 kb on + strand at 4452.279 kb on + strand at 4452.279 kb on + strand at 4452.279 kb on + strand at 4452.279 kb on + strand at 4452.300 kb on + strand at 4452.301 kb on - strand at 4453.326 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 4,450,329 + Pf1N1B4_4001 0.71 -0.3 4,450,329 + Pf1N1B4_4001 0.71 -1.5 4,450,330 - Pf1N1B4_4001 0.71 -1.1 4,450,584 + -0.6 4,450,584 + +0.4 4,450,584 + -1.5 4,450,584 + -0.0 4,450,585 - -0.1 4,450,585 - -0.3 4,450,585 - +0.5 4,450,585 - +0.5 4,450,585 - -0.7 4,450,585 - -0.5 4,451,233 - Pf1N1B4_4002 0.23 +0.5 4,451,367 + Pf1N1B4_4002 0.32 -0.3 4,451,367 + Pf1N1B4_4002 0.32 -0.5 4,451,368 - -0.1 4,451,368 - Pf1N1B4_4002 0.32 +0.1 4,451,481 + Pf1N1B4_4002 0.40 -0.4 4,451,481 + Pf1N1B4_4002 0.40 -0.6 4,451,482 - Pf1N1B4_4002 0.40 -1.0 4,451,853 + -0.3 4,451,854 - Pf1N1B4_4002 0.64 +1.3 4,452,162 + Pf1N1B4_4002 0.84 +2.0 4,452,162 + Pf1N1B4_4002 0.84 +0.9 4,452,162 + Pf1N1B4_4002 0.84 -1.2 4,452,162 + Pf1N1B4_4002 0.84 +0.9 4,452,162 + Pf1N1B4_4002 0.84 -0.6 4,452,162 + Pf1N1B4_4002 0.84 +2.2 4,452,163 - Pf1N1B4_4002 0.84 -0.4 4,452,163 - Pf1N1B4_4002 0.84 +0.1 4,452,163 - Pf1N1B4_4002 0.84 +0.8 4,452,163 - Pf1N1B4_4002 0.84 +1.7 4,452,179 + Pf1N1B4_4002 0.85 -2.8 4,452,186 + Pf1N1B4_4002 0.86 +3.1 4,452,186 + Pf1N1B4_4002 0.86 +0.3 4,452,279 + +0.5 4,452,279 + +1.2 4,452,279 + -1.9 4,452,279 + +1.3 4,452,279 + -1.1 4,452,300 + -0.6 4,452,301 - +0.5 4,453,326 - -0.1
Or see this region's nucleotide sequence