Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3525 and Pf1N1B4_3526 are separated by 50 nucleotides Pf1N1B4_3526 and Pf1N1B4_3527 are separated by 8 nucleotides Pf1N1B4_3527 and Pf1N1B4_3528 are separated by 68 nucleotides
Pf1N1B4_3525 - hydrolase, alpha/beta fold family, at 3,931,039 to 3,931,893
_3525
Pf1N1B4_3526 - Glutathione S-transferase (EC 2.5.1.18), at 3,931,944 to 3,932,603
_3526
Pf1N1B4_3527 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 3,932,612 to 3,933,418
_3527
Pf1N1B4_3528 - Putative membrane protein, at 3,933,487 to 3,934,542
_3528
Position (kb)
3932
3933
3934 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3931.629 kb on + strand, within Pf1N1B4_3525 at 3931.629 kb on + strand, within Pf1N1B4_3525 at 3931.630 kb on - strand at 3931.948 kb on + strand at 3931.948 kb on + strand at 3931.948 kb on + strand at 3931.949 kb on - strand at 3931.949 kb on - strand at 3931.956 kb on + strand at 3931.956 kb on + strand at 3932.164 kb on - strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.403 kb on + strand, within Pf1N1B4_3526 at 3932.427 kb on + strand, within Pf1N1B4_3526 at 3932.608 kb on - strand at 3932.672 kb on - strand at 3932.849 kb on + strand at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.932 kb on - strand, within Pf1N1B4_3527 at 3932.932 kb on - strand, within Pf1N1B4_3527 at 3933.005 kb on + strand, within Pf1N1B4_3527 at 3933.030 kb on - strand, within Pf1N1B4_3527 at 3933.030 kb on - strand, within Pf1N1B4_3527 at 3933.034 kb on - strand, within Pf1N1B4_3527 at 3933.080 kb on + strand, within Pf1N1B4_3527 at 3933.081 kb on - strand, within Pf1N1B4_3527 at 3933.110 kb on + strand, within Pf1N1B4_3527 at 3933.351 kb on + strand at 3933.454 kb on - strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.485 kb on - strand at 3933.485 kb on - strand at 3933.773 kb on + strand, within Pf1N1B4_3528 at 3933.774 kb on - strand, within Pf1N1B4_3528 at 3933.774 kb on - strand, within Pf1N1B4_3528 at 3933.774 kb on - strand, within Pf1N1B4_3528 at 3933.978 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.076 kb on - strand at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.353 kb on - strand at 3934.356 kb on + strand, within Pf1N1B4_3528 at 3934.356 kb on + strand at 3934.356 kb on + strand, within Pf1N1B4_3528 at 3934.356 kb on + strand, within Pf1N1B4_3528 at 3934.357 kb on - strand, within Pf1N1B4_3528
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 3,931,629 + Pf1N1B4_3525 0.69 +0.2 3,931,629 + Pf1N1B4_3525 0.69 -1.3 3,931,630 - -0.9 3,931,948 + -0.5 3,931,948 + -0.5 3,931,948 + +0.2 3,931,949 - +1.3 3,931,949 - +2.1 3,931,956 + -0.8 3,931,956 + -1.7 3,932,164 - Pf1N1B4_3526 0.33 -0.3 3,932,352 + Pf1N1B4_3526 0.62 +1.5 3,932,352 + Pf1N1B4_3526 0.62 -1.3 3,932,352 + Pf1N1B4_3526 0.62 +0.1 3,932,352 + Pf1N1B4_3526 0.62 +0.9 3,932,352 + Pf1N1B4_3526 0.62 -1.6 3,932,352 + Pf1N1B4_3526 0.62 -2.1 3,932,352 + Pf1N1B4_3526 0.62 +0.1 3,932,403 + Pf1N1B4_3526 0.70 -1.2 3,932,427 + Pf1N1B4_3526 0.73 +1.1 3,932,608 - +0.7 3,932,672 - +0.5 3,932,849 + -0.1 3,932,850 - Pf1N1B4_3527 0.29 +0.5 3,932,850 - Pf1N1B4_3527 0.29 -1.2 3,932,850 - Pf1N1B4_3527 0.29 +2.1 3,932,932 - Pf1N1B4_3527 0.40 +0.1 3,932,932 - Pf1N1B4_3527 0.40 -1.3 3,933,005 + Pf1N1B4_3527 0.49 -0.1 3,933,030 - Pf1N1B4_3527 0.52 -1.5 3,933,030 - Pf1N1B4_3527 0.52 +1.1 3,933,034 - Pf1N1B4_3527 0.52 -1.2 3,933,080 + Pf1N1B4_3527 0.58 +0.6 3,933,081 - Pf1N1B4_3527 0.58 +1.5 3,933,110 + Pf1N1B4_3527 0.62 -0.7 3,933,351 + -0.9 3,933,454 - +1.1 3,933,484 + +1.5 3,933,484 + +0.1 3,933,484 + +1.5 3,933,484 + +2.3 3,933,484 + +0.5 3,933,485 - -0.8 3,933,485 - -1.0 3,933,773 + Pf1N1B4_3528 0.27 +0.8 3,933,774 - Pf1N1B4_3528 0.27 +0.3 3,933,774 - Pf1N1B4_3528 0.27 +1.0 3,933,774 - Pf1N1B4_3528 0.27 -0.3 3,933,978 + Pf1N1B4_3528 0.46 -0.1 3,934,075 + Pf1N1B4_3528 0.56 +2.6 3,934,075 + Pf1N1B4_3528 0.56 -0.1 3,934,075 + Pf1N1B4_3528 0.56 +0.6 3,934,075 + Pf1N1B4_3528 0.56 +0.7 3,934,075 + Pf1N1B4_3528 0.56 +0.7 3,934,076 - +1.2 3,934,352 + Pf1N1B4_3528 0.82 +3.7 3,934,352 + Pf1N1B4_3528 0.82 -0.5 3,934,352 + -1.5 3,934,352 + Pf1N1B4_3528 0.82 -0.1 3,934,352 + Pf1N1B4_3528 0.82 +1.6 3,934,352 + Pf1N1B4_3528 0.82 +1.5 3,934,352 + Pf1N1B4_3528 0.82 +0.5 3,934,353 - +0.1 3,934,356 + Pf1N1B4_3528 0.82 +1.6 3,934,356 + +2.5 3,934,356 + Pf1N1B4_3528 0.82 -0.6 3,934,356 + Pf1N1B4_3528 0.82 -2.2 3,934,357 - Pf1N1B4_3528 0.82 +0.2
Or see this region's nucleotide sequence