Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2739

Experiment: Nitrate reduction, replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2738 and Pf1N1B4_2739 are separated by 90 nucleotidesPf1N1B4_2739 and Pf1N1B4_2740 are separated by 105 nucleotidesPf1N1B4_2740 and Pf1N1B4_2741 overlap by 4 nucleotides Pf1N1B4_2738 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 3,077,255 to 3,078,175 _2738 Pf1N1B4_2739 - acetyltransferase, GNAT family, at 3,078,266 to 3,078,835 _2739 Pf1N1B4_2740 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 3,078,941 to 3,079,810 _2740 Pf1N1B4_2741 - Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family, at 3,079,807 to 3,080,400 _2741 Position (kb) 3078 3079Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3077.487 kb on + strand, within Pf1N1B4_2738at 3077.487 kb on + strand, within Pf1N1B4_2738at 3077.487 kb on + strand, within Pf1N1B4_2738at 3077.488 kb on - strand, within Pf1N1B4_2738at 3077.488 kb on - strand, within Pf1N1B4_2738at 3077.509 kb on - strand, within Pf1N1B4_2738at 3077.523 kb on + strand, within Pf1N1B4_2738at 3077.523 kb on + strand, within Pf1N1B4_2738at 3077.523 kb on + strand, within Pf1N1B4_2738at 3077.524 kb on - strand, within Pf1N1B4_2738at 3077.524 kb on - strand, within Pf1N1B4_2738at 3077.524 kb on - strand, within Pf1N1B4_2738at 3077.553 kb on + strand, within Pf1N1B4_2738at 3077.553 kb on + strand, within Pf1N1B4_2738at 3077.554 kb on - strand, within Pf1N1B4_2738at 3077.751 kb on + strand, within Pf1N1B4_2738at 3077.751 kb on + strand, within Pf1N1B4_2738at 3077.805 kb on + strand, within Pf1N1B4_2738at 3077.805 kb on + strand, within Pf1N1B4_2738at 3077.805 kb on + strand, within Pf1N1B4_2738at 3077.806 kb on - strand, within Pf1N1B4_2738at 3077.806 kb on - strand, within Pf1N1B4_2738at 3077.976 kb on + strand, within Pf1N1B4_2738at 3077.976 kb on + strand, within Pf1N1B4_2738at 3077.976 kb on + strand, within Pf1N1B4_2738at 3077.976 kb on + strand, within Pf1N1B4_2738at 3077.976 kb on + strand, within Pf1N1B4_2738at 3077.977 kb on - strand, within Pf1N1B4_2738at 3077.977 kb on - strand, within Pf1N1B4_2738at 3077.977 kb on - strand, within Pf1N1B4_2738at 3077.977 kb on - strand, within Pf1N1B4_2738at 3077.987 kb on - strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strandat 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.988 kb on + strand, within Pf1N1B4_2738at 3077.989 kb on - strand, within Pf1N1B4_2738at 3077.989 kb on - strand, within Pf1N1B4_2738at 3078.144 kb on + strandat 3078.145 kb on - strandat 3078.145 kb on - strandat 3078.156 kb on + strandat 3078.156 kb on + strandat 3078.157 kb on - strandat 3078.157 kb on - strandat 3078.157 kb on - strandat 3078.184 kb on + strandat 3078.184 kb on + strandat 3078.184 kb on + strandat 3078.184 kb on + strandat 3078.185 kb on - strandat 3078.185 kb on - strandat 3078.185 kb on - strandat 3078.186 kb on + strandat 3078.186 kb on + strandat 3078.186 kb on + strandat 3078.200 kb on + strandat 3078.200 kb on + strandat 3078.200 kb on + strandat 3078.220 kb on + strandat 3078.220 kb on + strandat 3078.221 kb on - strandat 3078.231 kb on + strandat 3078.231 kb on + strandat 3078.231 kb on + strandat 3078.231 kb on + strandat 3078.231 kb on + strandat 3078.232 kb on - strandat 3078.232 kb on - strandat 3078.232 kb on - strandat 3078.232 kb on - strandat 3078.538 kb on + strand, within Pf1N1B4_2739at 3078.539 kb on - strand, within Pf1N1B4_2739at 3078.593 kb on + strand, within Pf1N1B4_2739at 3078.791 kb on + strandat 3078.792 kb on - strandat 3078.792 kb on - strandat 3078.833 kb on + strandat 3078.851 kb on + strandat 3078.851 kb on + strandat 3078.851 kb on + strandat 3078.851 kb on + strandat 3078.852 kb on - strandat 3078.852 kb on - strandat 3078.852 kb on - strandat 3078.852 kb on - strandat 3078.852 kb on - strandat 3078.879 kb on + strandat 3079.057 kb on - strand, within Pf1N1B4_2740at 3079.127 kb on + strand, within Pf1N1B4_2740at 3079.128 kb on - strand, within Pf1N1B4_2740at 3079.128 kb on - strand, within Pf1N1B4_2740at 3079.166 kb on + strand, within Pf1N1B4_2740at 3079.268 kb on + strand, within Pf1N1B4_2740at 3079.311 kb on + strand, within Pf1N1B4_2740at 3079.312 kb on - strand, within Pf1N1B4_2740at 3079.312 kb on - strand, within Pf1N1B4_2740at 3079.312 kb on - strand, within Pf1N1B4_2740at 3079.445 kb on + strand, within Pf1N1B4_2740at 3079.445 kb on + strand, within Pf1N1B4_2740at 3079.446 kb on - strand, within Pf1N1B4_2740at 3079.475 kb on + strand, within Pf1N1B4_2740at 3079.475 kb on + strand, within Pf1N1B4_2740at 3079.475 kb on + strand, within Pf1N1B4_2740at 3079.475 kb on + strand, within Pf1N1B4_2740at 3079.475 kb on + strand, within Pf1N1B4_2740at 3079.476 kb on - strand, within Pf1N1B4_2740at 3079.476 kb on - strand, within Pf1N1B4_2740at 3079.476 kb on - strand, within Pf1N1B4_2740at 3079.476 kb on - strand, within Pf1N1B4_2740at 3079.476 kb on - strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.530 kb on - strand, within Pf1N1B4_2740at 3079.530 kb on - strand, within Pf1N1B4_2740at 3079.530 kb on - strand, within Pf1N1B4_2740at 3079.530 kb on - strand, within Pf1N1B4_2740at 3079.530 kb on - strand, within Pf1N1B4_2740at 3079.680 kb on - strand, within Pf1N1B4_2740

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B
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3,077,487 + Pf1N1B4_2738 0.25 +1.1
3,077,487 + Pf1N1B4_2738 0.25 +0.5
3,077,487 + Pf1N1B4_2738 0.25 -1.5
3,077,488 - Pf1N1B4_2738 0.25 -1.3
3,077,488 - Pf1N1B4_2738 0.25 -0.9
3,077,509 - Pf1N1B4_2738 0.28 +0.3
3,077,523 + Pf1N1B4_2738 0.29 -1.1
3,077,523 + Pf1N1B4_2738 0.29 +0.5
3,077,523 + Pf1N1B4_2738 0.29 +0.1
3,077,524 - Pf1N1B4_2738 0.29 +0.7
3,077,524 - Pf1N1B4_2738 0.29 +0.0
3,077,524 - Pf1N1B4_2738 0.29 -0.8
3,077,553 + Pf1N1B4_2738 0.32 +2.5
3,077,553 + Pf1N1B4_2738 0.32 -1.8
3,077,554 - Pf1N1B4_2738 0.32 +0.9
3,077,751 + Pf1N1B4_2738 0.54 +0.5
3,077,751 + Pf1N1B4_2738 0.54 -0.3
3,077,805 + Pf1N1B4_2738 0.60 -0.4
3,077,805 + Pf1N1B4_2738 0.60 +0.8
3,077,805 + Pf1N1B4_2738 0.60 +1.7
3,077,806 - Pf1N1B4_2738 0.60 +1.5
3,077,806 - Pf1N1B4_2738 0.60 -1.5
3,077,976 + Pf1N1B4_2738 0.78 -1.0
3,077,976 + Pf1N1B4_2738 0.78 +2.3
3,077,976 + Pf1N1B4_2738 0.78 +0.5
3,077,976 + Pf1N1B4_2738 0.78 -0.2
3,077,976 + Pf1N1B4_2738 0.78 -0.5
3,077,977 - Pf1N1B4_2738 0.78 -0.1
3,077,977 - Pf1N1B4_2738 0.78 +1.0
3,077,977 - Pf1N1B4_2738 0.78 -0.7
3,077,977 - Pf1N1B4_2738 0.78 +1.3
3,077,987 - Pf1N1B4_2738 0.79 +0.7
3,077,988 + Pf1N1B4_2738 0.80 +0.2
3,077,988 + Pf1N1B4_2738 0.80 +0.7
3,077,988 + Pf1N1B4_2738 0.80 +0.5
3,077,988 + Pf1N1B4_2738 0.80 +1.0
3,077,988 + +0.9
3,077,988 + Pf1N1B4_2738 0.80 -1.1
3,077,988 + Pf1N1B4_2738 0.80 -0.3
3,077,988 + Pf1N1B4_2738 0.80 -2.2
3,077,988 + Pf1N1B4_2738 0.80 -0.2
3,077,988 + Pf1N1B4_2738 0.80 -0.1
3,077,988 + Pf1N1B4_2738 0.80 -1.5
3,077,988 + Pf1N1B4_2738 0.80 +1.1
3,077,988 + Pf1N1B4_2738 0.80 -1.3
3,077,988 + Pf1N1B4_2738 0.80 -1.3
3,077,989 - Pf1N1B4_2738 0.80 -0.4
3,077,989 - Pf1N1B4_2738 0.80 +0.5
3,078,144 + -0.5
3,078,145 - +0.5
3,078,145 - +0.5
3,078,156 + -1.0
3,078,156 + +1.5
3,078,157 - -1.5
3,078,157 - +0.4
3,078,157 - -1.5
3,078,184 + +2.3
3,078,184 + +0.0
3,078,184 + -0.5
3,078,184 + +1.6
3,078,185 - +0.8
3,078,185 - -0.2
3,078,185 - +0.1
3,078,186 + +2.7
3,078,186 + -0.2
3,078,186 + -2.6
3,078,200 + +1.5
3,078,200 + -0.9
3,078,200 + -1.4
3,078,220 + -0.1
3,078,220 + +1.9
3,078,221 - +1.5
3,078,231 + +0.5
3,078,231 + -0.1
3,078,231 + -1.1
3,078,231 + +2.9
3,078,231 + -0.1
3,078,232 - +1.0
3,078,232 - +0.1
3,078,232 - +0.5
3,078,232 - -1.2
3,078,538 + Pf1N1B4_2739 0.48 -0.5
3,078,539 - Pf1N1B4_2739 0.48 -1.0
3,078,593 + Pf1N1B4_2739 0.57 -1.2
3,078,791 + -0.7
3,078,792 - +0.5
3,078,792 - -0.1
3,078,833 + -0.1
3,078,851 + +0.4
3,078,851 + +1.0
3,078,851 + +0.5
3,078,851 + -0.5
3,078,852 - -0.1
3,078,852 - -1.3
3,078,852 - -1.7
3,078,852 - +0.3
3,078,852 - +0.9
3,078,879 + +0.5
3,079,057 - Pf1N1B4_2740 0.13 -0.9
3,079,127 + Pf1N1B4_2740 0.21 +0.5
3,079,128 - Pf1N1B4_2740 0.21 -0.6
3,079,128 - Pf1N1B4_2740 0.21 -1.3
3,079,166 + Pf1N1B4_2740 0.26 +0.7
3,079,268 + Pf1N1B4_2740 0.38 +0.9
3,079,311 + Pf1N1B4_2740 0.43 -1.2
3,079,312 - Pf1N1B4_2740 0.43 -0.0
3,079,312 - Pf1N1B4_2740 0.43 +0.4
3,079,312 - Pf1N1B4_2740 0.43 -1.0
3,079,445 + Pf1N1B4_2740 0.58 +0.2
3,079,445 + Pf1N1B4_2740 0.58 +0.1
3,079,446 - Pf1N1B4_2740 0.58 +2.9
3,079,475 + Pf1N1B4_2740 0.61 +0.9
3,079,475 + Pf1N1B4_2740 0.61 -1.0
3,079,475 + Pf1N1B4_2740 0.61 +1.8
3,079,475 + Pf1N1B4_2740 0.61 +1.7
3,079,475 + Pf1N1B4_2740 0.61 +3.0
3,079,476 - Pf1N1B4_2740 0.61 +0.8
3,079,476 - Pf1N1B4_2740 0.61 -1.9
3,079,476 - Pf1N1B4_2740 0.61 -0.2
3,079,476 - Pf1N1B4_2740 0.61 +0.5
3,079,476 - Pf1N1B4_2740 0.61 -2.2
3,079,529 + Pf1N1B4_2740 0.68 -0.9
3,079,529 + Pf1N1B4_2740 0.68 +2.0
3,079,529 + Pf1N1B4_2740 0.68 -0.5
3,079,529 + Pf1N1B4_2740 0.68 -1.9
3,079,529 + Pf1N1B4_2740 0.68 -1.0
3,079,529 + Pf1N1B4_2740 0.68 -0.3
3,079,529 + Pf1N1B4_2740 0.68 -0.1
3,079,529 + Pf1N1B4_2740 0.68 +3.0
3,079,529 + Pf1N1B4_2740 0.68 -0.0
3,079,529 + Pf1N1B4_2740 0.68 +0.9
3,079,530 - Pf1N1B4_2740 0.68 -0.7
3,079,530 - Pf1N1B4_2740 0.68 +0.9
3,079,530 - Pf1N1B4_2740 0.68 +0.2
3,079,530 - Pf1N1B4_2740 0.68 +2.6
3,079,530 - Pf1N1B4_2740 0.68 -1.5
3,079,680 - Pf1N1B4_2740 0.85 -1.1

Or see this region's nucleotide sequence