Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_233 and Pf1N1B4_234 are separated by 307 nucleotides Pf1N1B4_234 and Pf1N1B4_235 are separated by 65 nucleotides Pf1N1B4_235 and Pf1N1B4_236 are separated by 22 nucleotides Pf1N1B4_236 and Pf1N1B4_237 are separated by 81 nucleotides
Pf1N1B4_233 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 293,604 to 294,536
_233
Pf1N1B4_234 - hypothetical protein, at 294,844 to 295,152
_234
Pf1N1B4_235 - Molybdopterin biosynthesis protein MoeA, at 295,218 to 296,405
_235
Pf1N1B4_236 - Molybdenum cofactor biosynthesis protein MoaB, at 296,428 to 296,967
_236
Pf1N1B4_237 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 297,049 to 297,651
_237
Position (kb) 295
296
297 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 294.264 kb on + strand, within Pf1N1B4_233 at 294.265 kb on - strand at 294.351 kb on - strand, within Pf1N1B4_233 at 294.555 kb on + strand at 294.555 kb on + strand at 294.555 kb on + strand at 294.556 kb on - strand at 294.556 kb on - strand at 294.578 kb on + strand at 294.579 kb on - strand at 294.579 kb on - strand at 294.600 kb on + strand at 294.601 kb on - strand at 294.628 kb on + strand at 294.649 kb on - strand at 294.649 kb on - strand at 294.694 kb on + strand at 294.695 kb on - strand at 294.695 kb on - strand at 294.730 kb on + strand at 294.734 kb on + strand at 294.735 kb on - strand at 294.735 kb on - strand at 294.821 kb on + strand at 294.997 kb on + strand at 294.997 kb on + strand, within Pf1N1B4_234 at 294.997 kb on + strand, within Pf1N1B4_234 at 294.997 kb on + strand, within Pf1N1B4_234 at 294.998 kb on - strand, within Pf1N1B4_234 at 294.998 kb on - strand, within Pf1N1B4_234 at 295.150 kb on + strand at 295.150 kb on + strand at 295.150 kb on + strand at 295.216 kb on + strand at 295.217 kb on - strand at 295.275 kb on - strand at 295.348 kb on + strand, within Pf1N1B4_235 at 295.349 kb on - strand, within Pf1N1B4_235 at 295.387 kb on + strand, within Pf1N1B4_235 at 295.388 kb on - strand, within Pf1N1B4_235 at 295.388 kb on - strand, within Pf1N1B4_235 at 295.388 kb on - strand, within Pf1N1B4_235 at 295.483 kb on + strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.777 kb on - strand, within Pf1N1B4_235 at 295.812 kb on - strand, within Pf1N1B4_235 at 295.819 kb on + strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.949 kb on + strand, within Pf1N1B4_235 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.240 kb on - strand, within Pf1N1B4_235 at 296.638 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.838 kb on + strand, within Pf1N1B4_236 at 296.884 kb on - strand, within Pf1N1B4_236 at 296.947 kb on + strand at 296.948 kb on - strand at 296.948 kb on - strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.968 kb on - strand at 296.968 kb on - strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.984 kb on - strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.004 kb on - strand at 297.004 kb on - strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.006 kb on - strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.046 kb on - strand at 297.046 kb on - strand at 297.060 kb on + strand at 297.060 kb on + strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.238 kb on + strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.342 kb on - strand, within Pf1N1B4_237
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 294,264 + Pf1N1B4_233 0.71 -0.4 294,265 - -0.7 294,351 - Pf1N1B4_233 0.80 +0.5 294,555 + +0.3 294,555 + -2.2 294,555 + -1.5 294,556 - +0.9 294,556 - -0.2 294,578 + +1.5 294,579 - -1.7 294,579 - -1.1 294,600 + +1.2 294,601 - -0.7 294,628 + -0.0 294,649 - -0.6 294,649 - +0.6 294,694 + -1.9 294,695 - +1.0 294,695 - +1.1 294,730 + -0.9 294,734 + +0.7 294,735 - -0.6 294,735 - +0.1 294,821 + +0.5 294,997 + -1.7 294,997 + Pf1N1B4_234 0.50 -0.9 294,997 + Pf1N1B4_234 0.50 +1.5 294,997 + Pf1N1B4_234 0.50 -0.4 294,998 - Pf1N1B4_234 0.50 -2.1 294,998 - Pf1N1B4_234 0.50 -0.5 295,150 + +0.2 295,150 + -0.1 295,150 + -0.1 295,216 + +1.5 295,217 - +0.3 295,275 - -0.3 295,348 + Pf1N1B4_235 0.11 +0.2 295,349 - Pf1N1B4_235 0.11 +2.5 295,387 + Pf1N1B4_235 0.14 -0.5 295,388 - Pf1N1B4_235 0.14 -0.1 295,388 - Pf1N1B4_235 0.14 -0.9 295,388 - Pf1N1B4_235 0.14 +0.7 295,483 + Pf1N1B4_235 0.22 -1.7 295,735 - Pf1N1B4_235 0.44 -1.5 295,735 - Pf1N1B4_235 0.44 -0.9 295,735 - Pf1N1B4_235 0.44 -2.6 295,777 - Pf1N1B4_235 0.47 -1.1 295,812 - Pf1N1B4_235 0.50 -1.5 295,819 + Pf1N1B4_235 0.51 +0.5 295,820 - Pf1N1B4_235 0.51 -1.1 295,820 - Pf1N1B4_235 0.51 -1.7 295,820 - Pf1N1B4_235 0.51 -0.5 295,820 - Pf1N1B4_235 0.51 -1.1 295,820 - Pf1N1B4_235 0.51 -2.2 295,949 + Pf1N1B4_235 0.62 -2.6 296,053 - Pf1N1B4_235 0.70 -0.3 296,053 - Pf1N1B4_235 0.70 -1.1 296,148 - Pf1N1B4_235 0.78 -0.4 296,148 - Pf1N1B4_235 0.78 -3.0 296,240 - Pf1N1B4_235 0.86 -2.2 296,638 + Pf1N1B4_236 0.39 -0.5 296,821 + Pf1N1B4_236 0.73 -1.9 296,821 + Pf1N1B4_236 0.73 +0.1 296,821 + Pf1N1B4_236 0.73 -1.5 296,838 + Pf1N1B4_236 0.76 +0.3 296,884 - Pf1N1B4_236 0.84 -2.3 296,947 + -1.5 296,948 - -1.7 296,948 - +0.1 296,967 + -1.3 296,967 + -1.3 296,967 + -0.5 296,968 - -2.2 296,968 - -2.0 296,983 + -3.1 296,983 + -0.2 296,983 + -0.2 296,984 - -0.0 297,003 + -0.6 297,003 + +0.1 297,003 + -1.7 297,003 + -1.9 297,003 + +0.3 297,003 + -0.5 297,003 + +0.1 297,003 + -2.2 297,003 + -0.0 297,004 - -1.1 297,004 - +0.4 297,005 + -1.1 297,005 + -1.5 297,005 + -2.2 297,006 - -1.0 297,045 + +2.1 297,045 + +0.1 297,045 + -1.9 297,045 + -0.1 297,045 + +0.3 297,046 - -2.1 297,046 - -0.5 297,060 + -0.9 297,060 + -0.9 297,061 - -0.5 297,061 - -3.8 297,061 - -3.0 297,238 + Pf1N1B4_237 0.31 -0.9 297,239 - Pf1N1B4_237 0.32 -2.9 297,239 - Pf1N1B4_237 0.32 -2.3 297,239 - Pf1N1B4_237 0.32 -1.0 297,341 + Pf1N1B4_237 0.48 -1.5 297,341 + Pf1N1B4_237 0.48 -1.9 297,341 + Pf1N1B4_237 0.48 -0.3 297,342 - Pf1N1B4_237 0.49 -1.9
Or see this region's nucleotide sequence