Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_223 and Pf1N1B4_224 overlap by 4 nucleotides Pf1N1B4_224 and Pf1N1B4_225 are separated by 116 nucleotides Pf1N1B4_225 and Pf1N1B4_226 are separated by 71 nucleotides Pf1N1B4_226 and Pf1N1B4_227 are separated by 74 nucleotides
Pf1N1B4_223 - Citronellyl-CoA dehydrogenase, at 283,755 to 284,912
_223
Pf1N1B4_224 - Isohexenylglutaconyl-CoA hydratase, at 284,909 to 285,706
_224
Pf1N1B4_225 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 285,823 to 287,784
_225
Pf1N1B4_226 - Inhibitor of the KinA pathway to sporulation, predicted exonuclease, at 287,856 to 288,404
_226
Pf1N1B4_227 - FIG074102: hypothetical protein, at 288,479 to 288,763
_227
Position (kb) 285
286
287
288 Strain fitness (log2 ratio) -2
-1
0
1
2 at 285.288 kb on + strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.289 kb on - strand, within Pf1N1B4_224 at 285.806 kb on + strand at 285.806 kb on + strand at 285.806 kb on + strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.807 kb on - strand at 285.920 kb on + strand at 286.006 kb on + strand at 286.007 kb on - strand at 286.007 kb on - strand at 286.495 kb on + strand, within Pf1N1B4_225 at 286.495 kb on + strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.631 kb on - strand, within Pf1N1B4_225 at 286.682 kb on - strand, within Pf1N1B4_225 at 286.682 kb on - strand, within Pf1N1B4_225 at 286.846 kb on + strand, within Pf1N1B4_225 at 286.972 kb on + strand, within Pf1N1B4_225 at 286.972 kb on + strand, within Pf1N1B4_225 at 286.973 kb on - strand, within Pf1N1B4_225 at 287.234 kb on - strand, within Pf1N1B4_225 at 287.312 kb on + strand, within Pf1N1B4_225 at 287.391 kb on + strand, within Pf1N1B4_225 at 287.615 kb on + strand at 287.615 kb on + strand at 287.616 kb on - strand at 287.654 kb on + strand at 287.719 kb on - strand at 287.719 kb on - strand at 287.783 kb on - strand at 288.163 kb on - strand, within Pf1N1B4_226 at 288.362 kb on + strand at 288.363 kb on - strand at 288.440 kb on - strand at 288.484 kb on + strand at 288.484 kb on + strand at 288.498 kb on - strand at 288.740 kb on + strand at 288.740 kb on + strand at 288.740 kb on + strand at 288.740 kb on + strand at 288.741 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 285,288 + Pf1N1B4_224 0.47 -0.0 285,289 - Pf1N1B4_224 0.48 +0.8 285,289 - Pf1N1B4_224 0.48 +0.5 285,289 - Pf1N1B4_224 0.48 +1.4 285,806 + -0.3 285,806 + +0.7 285,806 + -0.2 285,807 - -1.2 285,807 - -0.7 285,807 - -0.4 285,807 - +2.0 285,807 - -0.6 285,807 - +0.3 285,920 + -0.3 286,006 + -0.8 286,007 - -1.4 286,007 - +1.4 286,495 + Pf1N1B4_225 0.34 -1.7 286,495 + Pf1N1B4_225 0.34 +1.1 286,631 - Pf1N1B4_225 0.41 +1.3 286,631 - Pf1N1B4_225 0.41 +0.2 286,631 - Pf1N1B4_225 0.41 +1.8 286,631 - Pf1N1B4_225 0.41 +0.8 286,682 - Pf1N1B4_225 0.44 -0.5 286,682 - Pf1N1B4_225 0.44 +0.7 286,846 + Pf1N1B4_225 0.52 -0.6 286,972 + Pf1N1B4_225 0.59 +0.5 286,972 + Pf1N1B4_225 0.59 -0.2 286,973 - Pf1N1B4_225 0.59 +0.4 287,234 - Pf1N1B4_225 0.72 +1.0 287,312 + Pf1N1B4_225 0.76 -1.1 287,391 + Pf1N1B4_225 0.80 -0.5 287,615 + +0.2 287,615 + -1.2 287,616 - +0.3 287,654 + +0.1 287,719 - -2.6 287,719 - -1.1 287,783 - -0.4 288,163 - Pf1N1B4_226 0.56 -2.3 288,362 + -1.1 288,363 - -1.3 288,440 - -1.7 288,484 + -0.1 288,484 + +2.3 288,498 - +0.2 288,740 + -0.5 288,740 + +0.1 288,740 + +0.0 288,740 + -1.0 288,741 - +0.5
Or see this region's nucleotide sequence