Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1575 and Pf1N1B4_1576 are separated by 104 nucleotides Pf1N1B4_1576 and Pf1N1B4_1577 are separated by 212 nucleotides
Pf1N1B4_1575 - Proline iminopeptidase (EC 3.4.11.5), at 1,814,036 to 1,815,007
_1575
Pf1N1B4_1576 - Lipolytic enzyme, at 1,815,112 to 1,816,014
_1576
Pf1N1B4_1577 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 1,816,227 to 1,817,183
_1577
Position (kb)
1815
1816
1817 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.311 kb on - strand, within Pf1N1B4_1575 at 1814.311 kb on - strand, within Pf1N1B4_1575 at 1814.311 kb on - strand, within Pf1N1B4_1575 at 1814.311 kb on - strand, within Pf1N1B4_1575 at 1814.486 kb on + strand, within Pf1N1B4_1575 at 1814.523 kb on + strand, within Pf1N1B4_1575 at 1814.523 kb on + strand, within Pf1N1B4_1575 at 1814.523 kb on + strand, within Pf1N1B4_1575 at 1814.523 kb on + strand, within Pf1N1B4_1575 at 1814.523 kb on + strand, within Pf1N1B4_1575 at 1814.524 kb on - strand, within Pf1N1B4_1575 at 1814.524 kb on - strand, within Pf1N1B4_1575 at 1814.524 kb on - strand, within Pf1N1B4_1575 at 1814.568 kb on + strand, within Pf1N1B4_1575 at 1814.568 kb on + strand, within Pf1N1B4_1575 at 1814.569 kb on - strand, within Pf1N1B4_1575 at 1814.653 kb on - strand, within Pf1N1B4_1575 at 1814.758 kb on + strand, within Pf1N1B4_1575 at 1814.934 kb on + strand at 1814.934 kb on + strand at 1814.935 kb on + strand at 1814.935 kb on - strand at 1814.935 kb on - strand at 1814.935 kb on - strand at 1814.935 kb on - strand at 1815.050 kb on + strand at 1815.050 kb on + strand at 1815.050 kb on + strand at 1815.050 kb on + strand at 1815.050 kb on + strand at 1815.050 kb on + strand at 1815.050 kb on + strand at 1815.051 kb on - strand at 1815.051 kb on - strand at 1815.051 kb on - strand at 1815.051 kb on - strand at 1815.051 kb on - strand at 1815.051 kb on - strand at 1815.051 kb on - strand at 1815.051 kb on - strand at 1815.054 kb on - strand at 1815.054 kb on - strand at 1815.073 kb on + strand at 1815.073 kb on + strand at 1815.073 kb on + strand at 1815.074 kb on - strand at 1815.074 kb on - strand at 1815.084 kb on + strand at 1815.084 kb on + strand at 1815.085 kb on - strand at 1815.085 kb on - strand at 1815.085 kb on - strand at 1815.085 kb on - strand at 1815.085 kb on - strand at 1815.085 kb on - strand at 1815.143 kb on + strand at 1815.162 kb on - strand at 1815.227 kb on + strand, within Pf1N1B4_1576 at 1815.230 kb on + strand, within Pf1N1B4_1576 at 1815.230 kb on + strand, within Pf1N1B4_1576 at 1815.231 kb on - strand, within Pf1N1B4_1576 at 1815.231 kb on - strand, within Pf1N1B4_1576 at 1815.369 kb on - strand, within Pf1N1B4_1576 at 1815.398 kb on + strand at 1815.398 kb on + strand, within Pf1N1B4_1576 at 1815.399 kb on - strand at 1815.399 kb on - strand, within Pf1N1B4_1576 at 1815.497 kb on + strand, within Pf1N1B4_1576 at 1815.497 kb on + strand, within Pf1N1B4_1576 at 1815.497 kb on + strand, within Pf1N1B4_1576 at 1815.523 kb on - strand, within Pf1N1B4_1576 at 1815.523 kb on - strand, within Pf1N1B4_1576 at 1815.803 kb on + strand, within Pf1N1B4_1576 at 1815.803 kb on + strand, within Pf1N1B4_1576 at 1815.804 kb on - strand, within Pf1N1B4_1576 at 1815.804 kb on - strand, within Pf1N1B4_1576 at 1816.061 kb on + strand at 1816.061 kb on + strand at 1816.061 kb on + strand at 1816.061 kb on + strand at 1816.062 kb on - strand at 1816.453 kb on + strand, within Pf1N1B4_1577 at 1816.454 kb on - strand, within Pf1N1B4_1577 at 1816.528 kb on + strand, within Pf1N1B4_1577 at 1816.528 kb on + strand, within Pf1N1B4_1577 at 1816.528 kb on + strand at 1816.528 kb on + strand, within Pf1N1B4_1577 at 1816.528 kb on + strand, within Pf1N1B4_1577 at 1816.528 kb on + strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.804 kb on + strand, within Pf1N1B4_1577 at 1816.804 kb on + strand, within Pf1N1B4_1577 at 1816.804 kb on + strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.898 kb on + strand, within Pf1N1B4_1577 at 1816.931 kb on + strand, within Pf1N1B4_1577
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 1,814,310 + Pf1N1B4_1575 0.28 -2.0 1,814,310 + Pf1N1B4_1575 0.28 -0.1 1,814,310 + Pf1N1B4_1575 0.28 -3.1 1,814,310 + Pf1N1B4_1575 0.28 -1.9 1,814,310 + Pf1N1B4_1575 0.28 +0.9 1,814,310 + Pf1N1B4_1575 0.28 -0.5 1,814,310 + Pf1N1B4_1575 0.28 -0.2 1,814,311 - Pf1N1B4_1575 0.28 +0.5 1,814,311 - Pf1N1B4_1575 0.28 +0.1 1,814,311 - Pf1N1B4_1575 0.28 +0.7 1,814,311 - Pf1N1B4_1575 0.28 -1.0 1,814,486 + Pf1N1B4_1575 0.46 -1.4 1,814,523 + Pf1N1B4_1575 0.50 -0.3 1,814,523 + Pf1N1B4_1575 0.50 -1.3 1,814,523 + Pf1N1B4_1575 0.50 -0.9 1,814,523 + Pf1N1B4_1575 0.50 +2.1 1,814,523 + Pf1N1B4_1575 0.50 -0.3 1,814,524 - Pf1N1B4_1575 0.50 -0.7 1,814,524 - Pf1N1B4_1575 0.50 -0.1 1,814,524 - Pf1N1B4_1575 0.50 +0.3 1,814,568 + Pf1N1B4_1575 0.55 -0.8 1,814,568 + Pf1N1B4_1575 0.55 -1.7 1,814,569 - Pf1N1B4_1575 0.55 -0.3 1,814,653 - Pf1N1B4_1575 0.63 +1.5 1,814,758 + Pf1N1B4_1575 0.74 +0.5 1,814,934 + +0.2 1,814,934 + -0.0 1,814,935 + +1.5 1,814,935 - -1.3 1,814,935 - -1.1 1,814,935 - -0.9 1,814,935 - -1.2 1,815,050 + +1.5 1,815,050 + +1.5 1,815,050 + -0.9 1,815,050 + +1.5 1,815,050 + -0.4 1,815,050 + +0.6 1,815,050 + -0.6 1,815,051 - +0.1 1,815,051 - -0.1 1,815,051 - -0.3 1,815,051 - -0.3 1,815,051 - -1.4 1,815,051 - -0.6 1,815,051 - +1.8 1,815,051 - -0.1 1,815,054 - -0.9 1,815,054 - +1.2 1,815,073 + -0.4 1,815,073 + +0.4 1,815,073 + +3.5 1,815,074 - +0.6 1,815,074 - +1.5 1,815,084 + -0.5 1,815,084 + -1.9 1,815,085 - +0.5 1,815,085 - -0.5 1,815,085 - +0.3 1,815,085 - +1.1 1,815,085 - +0.1 1,815,085 - -1.3 1,815,143 + -0.3 1,815,162 - +4.3 1,815,227 + Pf1N1B4_1576 0.13 +0.7 1,815,230 + Pf1N1B4_1576 0.13 -2.1 1,815,230 + Pf1N1B4_1576 0.13 -0.9 1,815,231 - Pf1N1B4_1576 0.13 -1.9 1,815,231 - Pf1N1B4_1576 0.13 -0.3 1,815,369 - Pf1N1B4_1576 0.28 -1.1 1,815,398 + +1.5 1,815,398 + Pf1N1B4_1576 0.32 -0.3 1,815,399 - +0.9 1,815,399 - Pf1N1B4_1576 0.32 +0.2 1,815,497 + Pf1N1B4_1576 0.43 +1.1 1,815,497 + Pf1N1B4_1576 0.43 +1.5 1,815,497 + Pf1N1B4_1576 0.43 +0.1 1,815,523 - Pf1N1B4_1576 0.46 -0.8 1,815,523 - Pf1N1B4_1576 0.46 -1.7 1,815,803 + Pf1N1B4_1576 0.77 -2.4 1,815,803 + Pf1N1B4_1576 0.77 -0.4 1,815,804 - Pf1N1B4_1576 0.77 +0.2 1,815,804 - Pf1N1B4_1576 0.77 -0.3 1,816,061 + -0.7 1,816,061 + +2.7 1,816,061 + +2.5 1,816,061 + +0.1 1,816,062 - +0.1 1,816,453 + Pf1N1B4_1577 0.24 -1.3 1,816,454 - Pf1N1B4_1577 0.24 -1.1 1,816,528 + Pf1N1B4_1577 0.31 -0.8 1,816,528 + Pf1N1B4_1577 0.31 +0.6 1,816,528 + -1.5 1,816,528 + Pf1N1B4_1577 0.31 -1.3 1,816,528 + Pf1N1B4_1577 0.31 -1.5 1,816,528 + Pf1N1B4_1577 0.31 -1.6 1,816,529 - Pf1N1B4_1577 0.32 -1.5 1,816,529 - Pf1N1B4_1577 0.32 -0.8 1,816,529 - Pf1N1B4_1577 0.32 +0.8 1,816,529 - Pf1N1B4_1577 0.32 -0.0 1,816,529 - Pf1N1B4_1577 0.32 +1.3 1,816,529 - Pf1N1B4_1577 0.32 -1.1 1,816,529 - Pf1N1B4_1577 0.32 -0.8 1,816,804 + Pf1N1B4_1577 0.60 +1.2 1,816,804 + Pf1N1B4_1577 0.60 -2.1 1,816,804 + Pf1N1B4_1577 0.60 -1.9 1,816,805 - Pf1N1B4_1577 0.60 -0.7 1,816,805 - Pf1N1B4_1577 0.60 +1.5 1,816,805 - Pf1N1B4_1577 0.60 -0.7 1,816,805 - Pf1N1B4_1577 0.60 -1.1 1,816,805 - Pf1N1B4_1577 0.60 -0.1 1,816,805 - Pf1N1B4_1577 0.60 -1.2 1,816,805 - Pf1N1B4_1577 0.60 -1.0 1,816,898 + Pf1N1B4_1577 0.70 -1.9 1,816,931 + Pf1N1B4_1577 0.74 -0.3
Or see this region's nucleotide sequence