Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1222 and Pf1N1B4_1223 are separated by 69 nucleotides Pf1N1B4_1223 and Pf1N1B4_1224 are separated by 65 nucleotides
Pf1N1B4_1222 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 1,386,353 to 1,387,276
_1222
Pf1N1B4_1223 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 1,387,346 to 1,388,428
_1223
Pf1N1B4_1224 - Probable protein farnesyltransferase beta subunit (EC 2.5.1.58), at 1,388,494 to 1,390,671
_1224
Position (kb)
1387
1388
1389 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1386.426 kb on + strand at 1386.426 kb on + strand at 1386.427 kb on - strand at 1386.427 kb on - strand at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.502 kb on - strand at 1386.502 kb on - strand, within Pf1N1B4_1222 at 1386.502 kb on - strand, within Pf1N1B4_1222 at 1386.657 kb on + strand, within Pf1N1B4_1222 at 1386.657 kb on + strand, within Pf1N1B4_1222 at 1386.741 kb on + strand, within Pf1N1B4_1222 at 1386.742 kb on - strand, within Pf1N1B4_1222 at 1386.742 kb on - strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.861 kb on + strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.864 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1387.162 kb on - strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.253 kb on + strand at 1387.266 kb on + strand at 1387.266 kb on + strand at 1387.266 kb on + strand at 1387.441 kb on - strand at 1387.530 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.783 kb on - strand, within Pf1N1B4_1223 at 1387.783 kb on - strand, within Pf1N1B4_1223 at 1387.861 kb on - strand, within Pf1N1B4_1223 at 1387.881 kb on - strand, within Pf1N1B4_1223 at 1387.971 kb on + strand, within Pf1N1B4_1223 at 1387.972 kb on - strand, within Pf1N1B4_1223 at 1387.972 kb on - strand, within Pf1N1B4_1223 at 1388.019 kb on + strand, within Pf1N1B4_1223 at 1388.019 kb on + strand, within Pf1N1B4_1223 at 1388.115 kb on + strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.128 kb on + strand, within Pf1N1B4_1223 at 1388.336 kb on + strand at 1388.336 kb on + strand at 1388.337 kb on - strand at 1388.337 kb on - strand at 1388.374 kb on - strand at 1388.660 kb on + strand at 1388.660 kb on + strand at 1388.660 kb on + strand at 1388.660 kb on + strand at 1388.660 kb on + strand at 1388.660 kb on + strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.684 kb on + strand at 1388.684 kb on + strand at 1388.684 kb on + strand at 1388.684 kb on + strand at 1388.684 kb on + strand at 1388.685 kb on - strand at 1388.685 kb on - strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.710 kb on - strand at 1388.815 kb on - strand, within Pf1N1B4_1224 at 1388.847 kb on - strand, within Pf1N1B4_1224 at 1389.012 kb on - strand, within Pf1N1B4_1224 at 1389.063 kb on - strand, within Pf1N1B4_1224 at 1389.128 kb on + strand, within Pf1N1B4_1224 at 1389.128 kb on + strand, within Pf1N1B4_1224 at 1389.128 kb on + strand, within Pf1N1B4_1224 at 1389.129 kb on - strand, within Pf1N1B4_1224 at 1389.139 kb on + strand, within Pf1N1B4_1224 at 1389.139 kb on + strand, within Pf1N1B4_1224 at 1389.139 kb on + strand, within Pf1N1B4_1224 at 1389.139 kb on + strand, within Pf1N1B4_1224 at 1389.140 kb on - strand, within Pf1N1B4_1224 at 1389.140 kb on - strand, within Pf1N1B4_1224 at 1389.140 kb on - strand, within Pf1N1B4_1224 at 1389.140 kb on - strand, within Pf1N1B4_1224 at 1389.140 kb on - strand, within Pf1N1B4_1224 at 1389.345 kb on + strand, within Pf1N1B4_1224 at 1389.345 kb on + strand, within Pf1N1B4_1224 at 1389.345 kb on + strand, within Pf1N1B4_1224 at 1389.345 kb on + strand, within Pf1N1B4_1224 at 1389.345 kb on + strand, within Pf1N1B4_1224 at 1389.346 kb on - strand, within Pf1N1B4_1224 at 1389.346 kb on - strand, within Pf1N1B4_1224 at 1389.346 kb on - strand at 1389.346 kb on - strand, within Pf1N1B4_1224
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 1,386,426 + +0.2 1,386,426 + +0.1 1,386,427 - -0.5 1,386,427 - +1.5 1,386,501 + Pf1N1B4_1222 0.16 -0.8 1,386,501 + Pf1N1B4_1222 0.16 -0.9 1,386,501 + Pf1N1B4_1222 0.16 +1.8 1,386,502 - +0.1 1,386,502 - Pf1N1B4_1222 0.16 +1.5 1,386,502 - Pf1N1B4_1222 0.16 +0.2 1,386,657 + Pf1N1B4_1222 0.33 +0.5 1,386,657 + Pf1N1B4_1222 0.33 +0.2 1,386,741 + Pf1N1B4_1222 0.42 -0.0 1,386,742 - Pf1N1B4_1222 0.42 +1.0 1,386,742 - Pf1N1B4_1222 0.42 +0.9 1,386,747 + Pf1N1B4_1222 0.43 +0.9 1,386,747 + Pf1N1B4_1222 0.43 -0.8 1,386,747 + Pf1N1B4_1222 0.43 +0.5 1,386,747 + Pf1N1B4_1222 0.43 -0.2 1,386,747 + Pf1N1B4_1222 0.43 +0.1 1,386,747 + Pf1N1B4_1222 0.43 -1.3 1,386,747 + Pf1N1B4_1222 0.43 -0.2 1,386,748 - Pf1N1B4_1222 0.43 +0.7 1,386,748 - Pf1N1B4_1222 0.43 -0.9 1,386,748 - Pf1N1B4_1222 0.43 -0.4 1,386,748 - Pf1N1B4_1222 0.43 -0.2 1,386,748 - Pf1N1B4_1222 0.43 +0.6 1,386,748 - Pf1N1B4_1222 0.43 -1.3 1,386,748 - Pf1N1B4_1222 0.43 +0.5 1,386,861 + Pf1N1B4_1222 0.55 -0.7 1,386,862 - Pf1N1B4_1222 0.55 +0.6 1,386,862 - Pf1N1B4_1222 0.55 +0.9 1,386,862 - Pf1N1B4_1222 0.55 +0.0 1,386,862 - Pf1N1B4_1222 0.55 +1.5 1,386,864 + Pf1N1B4_1222 0.55 -0.8 1,386,962 + Pf1N1B4_1222 0.66 -1.1 1,386,962 + Pf1N1B4_1222 0.66 -0.4 1,386,962 + Pf1N1B4_1222 0.66 -0.8 1,386,962 + Pf1N1B4_1222 0.66 -0.6 1,386,962 + Pf1N1B4_1222 0.66 +2.6 1,386,962 + +0.9 1,386,962 + Pf1N1B4_1222 0.66 -0.4 1,386,962 + Pf1N1B4_1222 0.66 -0.4 1,386,962 + Pf1N1B4_1222 0.66 +0.8 1,386,962 + Pf1N1B4_1222 0.66 -0.3 1,386,962 + Pf1N1B4_1222 0.66 +0.6 1,386,962 + Pf1N1B4_1222 0.66 +1.5 1,386,962 + Pf1N1B4_1222 0.66 -2.2 1,386,962 + Pf1N1B4_1222 0.66 -1.9 1,386,963 - Pf1N1B4_1222 0.66 -2.2 1,386,963 - Pf1N1B4_1222 0.66 +0.1 1,386,963 - Pf1N1B4_1222 0.66 +0.9 1,386,963 - Pf1N1B4_1222 0.66 -0.1 1,386,963 - Pf1N1B4_1222 0.66 -0.9 1,386,963 - Pf1N1B4_1222 0.66 +1.0 1,386,963 - Pf1N1B4_1222 0.66 -0.5 1,386,963 - -0.9 1,386,963 - Pf1N1B4_1222 0.66 -1.2 1,386,963 - Pf1N1B4_1222 0.66 +0.1 1,386,963 - Pf1N1B4_1222 0.66 +1.1 1,386,963 - Pf1N1B4_1222 0.66 +0.5 1,386,963 - Pf1N1B4_1222 0.66 +0.7 1,386,963 - Pf1N1B4_1222 0.66 +1.2 1,386,963 - Pf1N1B4_1222 0.66 +1.1 1,386,963 - Pf1N1B4_1222 0.66 +1.7 1,386,963 - Pf1N1B4_1222 0.66 +0.5 1,386,963 - Pf1N1B4_1222 0.66 -0.9 1,386,963 - Pf1N1B4_1222 0.66 -0.1 1,387,162 - Pf1N1B4_1222 0.88 -0.7 1,387,164 + Pf1N1B4_1222 0.88 +1.8 1,387,164 + Pf1N1B4_1222 0.88 -1.1 1,387,164 + Pf1N1B4_1222 0.88 +0.2 1,387,164 + Pf1N1B4_1222 0.88 -1.3 1,387,165 - Pf1N1B4_1222 0.88 +0.7 1,387,165 - Pf1N1B4_1222 0.88 -0.2 1,387,165 - Pf1N1B4_1222 0.88 +0.2 1,387,165 - Pf1N1B4_1222 0.88 +1.5 1,387,165 - Pf1N1B4_1222 0.88 -0.5 1,387,165 - Pf1N1B4_1222 0.88 -0.3 1,387,165 - Pf1N1B4_1222 0.88 -1.1 1,387,253 + +0.2 1,387,266 + +1.7 1,387,266 + -0.5 1,387,266 + +1.5 1,387,441 - +1.5 1,387,530 + Pf1N1B4_1223 0.17 -0.4 1,387,686 + Pf1N1B4_1223 0.31 -0.5 1,387,686 + Pf1N1B4_1223 0.31 +0.0 1,387,686 + Pf1N1B4_1223 0.31 -0.9 1,387,686 + Pf1N1B4_1223 0.31 -0.1 1,387,686 + Pf1N1B4_1223 0.31 -0.6 1,387,686 + Pf1N1B4_1223 0.31 +2.1 1,387,686 + Pf1N1B4_1223 0.31 -0.7 1,387,686 + Pf1N1B4_1223 0.31 +0.7 1,387,687 - Pf1N1B4_1223 0.31 -0.9 1,387,687 - Pf1N1B4_1223 0.31 -0.5 1,387,687 - Pf1N1B4_1223 0.31 -0.2 1,387,687 - Pf1N1B4_1223 0.31 +0.3 1,387,687 - Pf1N1B4_1223 0.31 +2.8 1,387,687 - Pf1N1B4_1223 0.31 +0.5 1,387,687 - Pf1N1B4_1223 0.31 -0.1 1,387,722 + Pf1N1B4_1223 0.35 -0.9 1,387,722 + Pf1N1B4_1223 0.35 +0.1 1,387,722 + Pf1N1B4_1223 0.35 -0.8 1,387,722 + Pf1N1B4_1223 0.35 -0.5 1,387,722 + Pf1N1B4_1223 0.35 +0.7 1,387,723 - Pf1N1B4_1223 0.35 -1.6 1,387,723 - Pf1N1B4_1223 0.35 +0.8 1,387,723 - Pf1N1B4_1223 0.35 -0.7 1,387,723 - Pf1N1B4_1223 0.35 +1.5 1,387,783 - Pf1N1B4_1223 0.40 +0.1 1,387,783 - Pf1N1B4_1223 0.40 +2.5 1,387,861 - Pf1N1B4_1223 0.48 +0.2 1,387,881 - Pf1N1B4_1223 0.49 +1.2 1,387,971 + Pf1N1B4_1223 0.58 +2.5 1,387,972 - Pf1N1B4_1223 0.58 +1.3 1,387,972 - Pf1N1B4_1223 0.58 -0.9 1,388,019 + Pf1N1B4_1223 0.62 -1.9 1,388,019 + Pf1N1B4_1223 0.62 -0.5 1,388,115 + Pf1N1B4_1223 0.71 +1.5 1,388,116 - Pf1N1B4_1223 0.71 -0.9 1,388,116 - Pf1N1B4_1223 0.71 -0.6 1,388,116 - Pf1N1B4_1223 0.71 +1.1 1,388,128 + Pf1N1B4_1223 0.72 -2.0 1,388,336 + -0.2 1,388,336 + +1.7 1,388,337 - +0.6 1,388,337 - -0.3 1,388,374 - +0.1 1,388,660 + +1.5 1,388,660 + -0.1 1,388,660 + +0.3 1,388,660 + -1.1 1,388,660 + +1.7 1,388,660 + -0.3 1,388,661 - -0.1 1,388,661 - -1.3 1,388,661 - -0.3 1,388,661 - -0.9 1,388,661 - -0.1 1,388,661 - -0.1 1,388,661 - +1.2 1,388,684 + -1.0 1,388,684 + -0.3 1,388,684 + -0.8 1,388,684 + -0.6 1,388,684 + +0.4 1,388,685 - -0.8 1,388,685 - +0.1 1,388,705 + -1.5 1,388,705 + -0.3 1,388,705 + -0.2 1,388,705 + -0.1 1,388,705 + -1.3 1,388,705 + -0.7 1,388,705 + +0.3 1,388,705 + -1.3 1,388,706 - -0.9 1,388,706 - +1.1 1,388,706 - +1.2 1,388,706 - +1.9 1,388,706 - +0.5 1,388,706 - +0.6 1,388,706 - -1.1 1,388,706 - +0.9 1,388,706 - -1.8 1,388,706 - -0.8 1,388,706 - +1.1 1,388,706 - -1.9 1,388,706 - +0.5 1,388,706 - +0.6 1,388,706 - +0.7 1,388,706 - -0.5 1,388,706 - +0.1 1,388,706 - +1.1 1,388,706 - +0.4 1,388,706 - -0.5 1,388,706 - +1.0 1,388,706 - -1.1 1,388,706 - +0.3 1,388,706 - +0.3 1,388,706 - -0.0 1,388,710 - +0.7 1,388,815 - Pf1N1B4_1224 0.15 -0.9 1,388,847 - Pf1N1B4_1224 0.16 -0.3 1,389,012 - Pf1N1B4_1224 0.24 +1.0 1,389,063 - Pf1N1B4_1224 0.26 -2.3 1,389,128 + Pf1N1B4_1224 0.29 -1.5 1,389,128 + Pf1N1B4_1224 0.29 +0.0 1,389,128 + Pf1N1B4_1224 0.29 -0.5 1,389,129 - Pf1N1B4_1224 0.29 +0.9 1,389,139 + Pf1N1B4_1224 0.30 -1.7 1,389,139 + Pf1N1B4_1224 0.30 +1.5 1,389,139 + Pf1N1B4_1224 0.30 +1.9 1,389,139 + Pf1N1B4_1224 0.30 +1.5 1,389,140 - Pf1N1B4_1224 0.30 -0.1 1,389,140 - Pf1N1B4_1224 0.30 +0.7 1,389,140 - Pf1N1B4_1224 0.30 +0.6 1,389,140 - Pf1N1B4_1224 0.30 -0.1 1,389,140 - Pf1N1B4_1224 0.30 -0.9 1,389,345 + Pf1N1B4_1224 0.39 +1.5 1,389,345 + Pf1N1B4_1224 0.39 -0.4 1,389,345 + Pf1N1B4_1224 0.39 +0.1 1,389,345 + Pf1N1B4_1224 0.39 -1.1 1,389,345 + Pf1N1B4_1224 0.39 +0.0 1,389,346 - Pf1N1B4_1224 0.39 +0.1 1,389,346 - Pf1N1B4_1224 0.39 -1.0 1,389,346 - +0.5 1,389,346 - Pf1N1B4_1224 0.39 -1.1
Or see this region's nucleotide sequence