Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5031 and Pf1N1B4_5032 are separated by 75 nucleotides Pf1N1B4_5032 and Pf1N1B4_5033 are separated by 69 nucleotides Pf1N1B4_5033 and Pf1N1B4_5034 are separated by 131 nucleotides Pf1N1B4_5034 and Pf1N1B4_5035 are separated by 306 nucleotides
Pf1N1B4_5031 - Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1), at 5,576,918 to 5,577,997
_5031
Pf1N1B4_5032 - Hypothetical protein PA2244 (similar to DNA topoisomerase IB, but possibly involved in glycosyl-transfer), at 5,578,073 to 5,579,110
_5032
Pf1N1B4_5033 - hypothetical protein, at 5,579,180 to 5,579,437
_5033
Pf1N1B4_5034 - hypothetical protein, at 5,579,569 to 5,579,799
_5034
Pf1N1B4_5035 - hypothetical protein, at 5,580,106 to 5,580,390
_5035
Position (kb) 5578
5579
5580 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5577.375 kb on + strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.462 kb on + strand at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.463 kb on - strand, within Pf1N1B4_5031 at 5577.650 kb on + strand, within Pf1N1B4_5031 at 5577.868 kb on - strand, within Pf1N1B4_5031 at 5578.037 kb on + strand at 5578.119 kb on - strand at 5578.376 kb on + strand, within Pf1N1B4_5032 at 5578.377 kb on - strand, within Pf1N1B4_5032 at 5578.487 kb on - strand, within Pf1N1B4_5032 at 5578.487 kb on - strand, within Pf1N1B4_5032 at 5578.530 kb on - strand, within Pf1N1B4_5032 at 5578.772 kb on + strand, within Pf1N1B4_5032 at 5578.776 kb on + strand, within Pf1N1B4_5032 at 5578.777 kb on - strand at 5578.964 kb on + strand, within Pf1N1B4_5032 at 5578.965 kb on - strand at 5578.965 kb on - strand, within Pf1N1B4_5032 at 5579.035 kb on + strand at 5579.036 kb on - strand at 5579.036 kb on - strand at 5579.036 kb on - strand at 5579.130 kb on - strand at 5579.179 kb on + strand at 5579.179 kb on + strand at 5579.179 kb on + strand at 5579.180 kb on - strand at 5579.194 kb on + strand at 5579.194 kb on + strand at 5579.194 kb on + strand at 5579.194 kb on + strand at 5579.200 kb on + strand at 5579.200 kb on + strand at 5579.200 kb on + strand at 5579.200 kb on + strand at 5579.201 kb on - strand at 5579.201 kb on - strand at 5579.564 kb on - strand at 5579.772 kb on - strand, within Pf1N1B4_5034 at 5579.875 kb on + strand at 5579.876 kb on - strand at 5579.876 kb on - strand at 5580.044 kb on + strand at 5580.044 kb on + strand at 5580.044 kb on + strand at 5580.045 kb on - strand at 5580.045 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 remove 5,577,375 + Pf1N1B4_5031 0.42 -1.5 5,577,376 - Pf1N1B4_5031 0.42 -2.3 5,577,376 - Pf1N1B4_5031 0.42 +0.8 5,577,376 - Pf1N1B4_5031 0.42 +1.0 5,577,462 + Pf1N1B4_5031 0.50 +1.0 5,577,462 + -1.9 5,577,462 + Pf1N1B4_5031 0.50 -0.3 5,577,462 + Pf1N1B4_5031 0.50 -1.8 5,577,463 - Pf1N1B4_5031 0.50 -1.6 5,577,650 + Pf1N1B4_5031 0.68 -0.5 5,577,868 - Pf1N1B4_5031 0.88 -2.5 5,578,037 + -2.0 5,578,119 - -0.6 5,578,376 + Pf1N1B4_5032 0.29 -1.9 5,578,377 - Pf1N1B4_5032 0.29 -0.6 5,578,487 - Pf1N1B4_5032 0.40 -0.0 5,578,487 - Pf1N1B4_5032 0.40 -0.9 5,578,530 - Pf1N1B4_5032 0.44 -2.0 5,578,772 + Pf1N1B4_5032 0.67 +0.3 5,578,776 + Pf1N1B4_5032 0.68 -1.0 5,578,777 - -1.8 5,578,964 + Pf1N1B4_5032 0.86 -0.2 5,578,965 - -1.3 5,578,965 - Pf1N1B4_5032 0.86 +0.4 5,579,035 + -1.8 5,579,036 - +0.2 5,579,036 - -1.5 5,579,036 - +1.1 5,579,130 - -2.2 5,579,179 + -1.5 5,579,179 + -0.5 5,579,179 + +0.5 5,579,180 - -0.3 5,579,194 + -1.4 5,579,194 + +0.9 5,579,194 + -0.2 5,579,194 + -0.4 5,579,200 + +0.1 5,579,200 + +0.5 5,579,200 + -0.0 5,579,200 + -1.6 5,579,201 - +1.9 5,579,201 - +0.5 5,579,564 - -0.3 5,579,772 - Pf1N1B4_5034 0.88 -0.6 5,579,875 + -0.3 5,579,876 - +0.8 5,579,876 - -2.2 5,580,044 + +0.6 5,580,044 + +0.3 5,580,044 + +0.9 5,580,045 - +0.6 5,580,045 - -1.3
Or see this region's nucleotide sequence