Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_483

Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_482 and Pf1N1B4_483 are separated by 135 nucleotidesPf1N1B4_483 and Pf1N1B4_484 are separated by 92 nucleotidesPf1N1B4_484 and Pf1N1B4_485 are separated by 84 nucleotides Pf1N1B4_482 - Transcriptional regulator, LysR family, at 573,824 to 574,822 _482 Pf1N1B4_483 - Metal-dependent hydrolase related to alanyl-tRNA synthetase, at 574,958 to 575,539 _483 Pf1N1B4_484 - Hydrogenase maturation factor, at 575,632 to 576,402 _484 Pf1N1B4_485 - L-lysine permease, at 576,487 to 577,041 _485 Position (kb) 574 575 576Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 574.083 kb on + strand, within Pf1N1B4_482at 574.233 kb on + strand, within Pf1N1B4_482at 574.233 kb on + strand, within Pf1N1B4_482at 574.234 kb on - strand, within Pf1N1B4_482at 574.234 kb on - strand, within Pf1N1B4_482at 574.237 kb on + strand, within Pf1N1B4_482at 574.237 kb on + strand, within Pf1N1B4_482at 574.238 kb on - strand, within Pf1N1B4_482at 574.238 kb on - strand, within Pf1N1B4_482at 574.238 kb on - strand, within Pf1N1B4_482at 574.238 kb on - strand, within Pf1N1B4_482at 574.238 kb on - strand, within Pf1N1B4_482at 574.238 kb on - strand, within Pf1N1B4_482at 574.238 kb on - strand, within Pf1N1B4_482at 574.238 kb on - strand, within Pf1N1B4_482at 574.313 kb on + strand, within Pf1N1B4_482at 574.313 kb on + strand, within Pf1N1B4_482at 574.314 kb on - strand, within Pf1N1B4_482at 574.314 kb on - strand, within Pf1N1B4_482at 574.314 kb on - strand, within Pf1N1B4_482at 574.727 kb on + strandat 574.727 kb on + strandat 574.728 kb on - strandat 574.728 kb on - strandat 574.728 kb on - strandat 574.882 kb on - strandat 574.888 kb on - strandat 575.451 kb on + strand, within Pf1N1B4_483at 575.451 kb on + strand, within Pf1N1B4_483at 575.451 kb on + strand, within Pf1N1B4_483at 575.451 kb on + strand, within Pf1N1B4_483at 575.452 kb on - strand, within Pf1N1B4_483at 575.526 kb on - strandat 575.526 kb on - strandat 575.526 kb on - strandat 575.526 kb on - strandat 575.615 kb on + strandat 575.615 kb on + strandat 575.615 kb on + strandat 575.625 kb on + strandat 575.625 kb on + strandat 575.625 kb on + strandat 575.626 kb on - strandat 575.669 kb on + strandat 575.669 kb on + strandat 575.669 kb on + strandat 575.670 kb on - strandat 575.779 kb on + strand, within Pf1N1B4_484at 575.779 kb on + strand, within Pf1N1B4_484at 575.779 kb on + strand, within Pf1N1B4_484at 575.779 kb on + strand, within Pf1N1B4_484at 575.779 kb on + strand, within Pf1N1B4_484at 575.780 kb on - strand, within Pf1N1B4_484at 575.780 kb on - strand, within Pf1N1B4_484at 575.780 kb on - strand, within Pf1N1B4_484at 575.805 kb on + strand, within Pf1N1B4_484at 575.805 kb on + strand, within Pf1N1B4_484at 575.821 kb on + strand, within Pf1N1B4_484at 575.821 kb on + strand, within Pf1N1B4_484at 575.821 kb on + strand, within Pf1N1B4_484at 575.822 kb on - strand, within Pf1N1B4_484at 575.857 kb on + strand, within Pf1N1B4_484at 575.857 kb on + strand, within Pf1N1B4_484at 575.857 kb on + strand, within Pf1N1B4_484at 575.912 kb on - strand, within Pf1N1B4_484at 575.912 kb on - strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strandat 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.001 kb on + strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.002 kb on - strand, within Pf1N1B4_484at 576.127 kb on + strand, within Pf1N1B4_484at 576.127 kb on + strand, within Pf1N1B4_484at 576.239 kb on + strand, within Pf1N1B4_484at 576.239 kb on + strandat 576.240 kb on - strand, within Pf1N1B4_484at 576.416 kb on + strandat 576.416 kb on + strandat 576.416 kb on + strandat 576.417 kb on - strandat 576.417 kb on - strandat 576.521 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
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574,083 + Pf1N1B4_482 0.26 -1.1
574,233 + Pf1N1B4_482 0.41 +1.1
574,233 + Pf1N1B4_482 0.41 +1.9
574,234 - Pf1N1B4_482 0.41 -0.0
574,234 - Pf1N1B4_482 0.41 +0.5
574,237 + Pf1N1B4_482 0.41 +1.0
574,237 + Pf1N1B4_482 0.41 -0.3
574,238 - Pf1N1B4_482 0.41 +1.2
574,238 - Pf1N1B4_482 0.41 -0.2
574,238 - Pf1N1B4_482 0.41 +0.5
574,238 - Pf1N1B4_482 0.41 -0.5
574,238 - Pf1N1B4_482 0.41 -0.8
574,238 - Pf1N1B4_482 0.41 -0.3
574,238 - Pf1N1B4_482 0.41 -0.7
574,238 - Pf1N1B4_482 0.41 +2.5
574,313 + Pf1N1B4_482 0.49 +0.0
574,313 + Pf1N1B4_482 0.49 +0.0
574,314 - Pf1N1B4_482 0.49 -1.6
574,314 - Pf1N1B4_482 0.49 -0.3
574,314 - Pf1N1B4_482 0.49 -0.8
574,727 + -0.7
574,727 + -0.6
574,728 - +1.5
574,728 - -0.0
574,728 - +3.1
574,882 - -0.8
574,888 - -0.0
575,451 + Pf1N1B4_483 0.85 +0.4
575,451 + Pf1N1B4_483 0.85 -0.7
575,451 + Pf1N1B4_483 0.85 -0.5
575,451 + Pf1N1B4_483 0.85 -1.1
575,452 - Pf1N1B4_483 0.85 -2.2
575,526 - +0.7
575,526 - -0.7
575,526 - -0.1
575,526 - +0.4
575,615 + -0.8
575,615 + +2.5
575,615 + -0.3
575,625 + -1.0
575,625 + +1.1
575,625 + -0.1
575,626 - -0.5
575,669 + -0.5
575,669 + -0.5
575,669 + +0.8
575,670 - +1.0
575,779 + Pf1N1B4_484 0.19 +0.1
575,779 + Pf1N1B4_484 0.19 -0.5
575,779 + Pf1N1B4_484 0.19 -0.6
575,779 + Pf1N1B4_484 0.19 -0.6
575,779 + Pf1N1B4_484 0.19 -0.1
575,780 - Pf1N1B4_484 0.19 -0.3
575,780 - Pf1N1B4_484 0.19 +1.5
575,780 - Pf1N1B4_484 0.19 -0.5
575,805 + Pf1N1B4_484 0.22 +0.4
575,805 + Pf1N1B4_484 0.22 +0.1
575,821 + Pf1N1B4_484 0.25 +1.0
575,821 + Pf1N1B4_484 0.25 -2.5
575,821 + Pf1N1B4_484 0.25 -0.4
575,822 - Pf1N1B4_484 0.25 -0.4
575,857 + Pf1N1B4_484 0.29 +0.8
575,857 + Pf1N1B4_484 0.29 +1.1
575,857 + Pf1N1B4_484 0.29 +0.3
575,912 - Pf1N1B4_484 0.36 -0.2
575,912 - Pf1N1B4_484 0.36 -0.2
576,001 + Pf1N1B4_484 0.48 +0.7
576,001 + Pf1N1B4_484 0.48 +1.5
576,001 + Pf1N1B4_484 0.48 -0.7
576,001 + Pf1N1B4_484 0.48 -0.1
576,001 + Pf1N1B4_484 0.48 -0.5
576,001 + Pf1N1B4_484 0.48 +0.1
576,001 + +0.5
576,001 + Pf1N1B4_484 0.48 +0.2
576,001 + Pf1N1B4_484 0.48 +0.5
576,001 + Pf1N1B4_484 0.48 +0.4
576,001 + Pf1N1B4_484 0.48 -1.4
576,001 + Pf1N1B4_484 0.48 +0.9
576,001 + Pf1N1B4_484 0.48 +0.2
576,001 + Pf1N1B4_484 0.48 +0.4
576,001 + Pf1N1B4_484 0.48 -2.1
576,001 + Pf1N1B4_484 0.48 -1.5
576,001 + Pf1N1B4_484 0.48 +1.3
576,001 + Pf1N1B4_484 0.48 +0.5
576,001 + Pf1N1B4_484 0.48 +0.3
576,001 + Pf1N1B4_484 0.48 +1.5
576,001 + Pf1N1B4_484 0.48 -2.4
576,001 + Pf1N1B4_484 0.48 +0.2
576,001 + Pf1N1B4_484 0.48 +1.0
576,001 + Pf1N1B4_484 0.48 -1.5
576,002 - Pf1N1B4_484 0.48 -0.3
576,002 - Pf1N1B4_484 0.48 -0.8
576,002 - Pf1N1B4_484 0.48 +1.2
576,002 - Pf1N1B4_484 0.48 +0.7
576,002 - Pf1N1B4_484 0.48 -0.9
576,002 - Pf1N1B4_484 0.48 -0.6
576,002 - Pf1N1B4_484 0.48 +0.2
576,002 - Pf1N1B4_484 0.48 -0.5
576,002 - Pf1N1B4_484 0.48 -0.8
576,002 - Pf1N1B4_484 0.48 +0.2
576,002 - Pf1N1B4_484 0.48 -1.6
576,002 - Pf1N1B4_484 0.48 +0.3
576,002 - Pf1N1B4_484 0.48 +0.6
576,002 - Pf1N1B4_484 0.48 -0.9
576,127 + Pf1N1B4_484 0.64 -1.0
576,127 + Pf1N1B4_484 0.64 -0.5
576,239 + Pf1N1B4_484 0.79 -0.4
576,239 + -0.8
576,240 - Pf1N1B4_484 0.79 -1.0
576,416 + -0.7
576,416 + -2.4
576,416 + -0.6
576,417 - -3.2
576,417 - -2.2
576,521 + -0.2

Or see this region's nucleotide sequence