Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4002

Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
4,450,329	+		Pf1N1B4_4001	0.71	-0.2
4,450,329	+		Pf1N1B4_4001	0.71	-1.4
4,450,330	-		Pf1N1B4_4001	0.71	-2.0
4,450,584	+				-1.1
4,450,584	+				+0.5
4,450,584	+				-0.1
4,450,584	+				-0.5
4,450,585	-				-0.4
4,450,585	-				-0.2
4,450,585	-				+1.0
4,450,585	-				+0.0
4,450,585	-				-1.2
4,450,585	-				-0.7
4,451,233	-		Pf1N1B4_4002	0.23	-0.4
4,451,367	+		Pf1N1B4_4002	0.32	-0.8
4,451,367	+		Pf1N1B4_4002	0.32	-1.4
4,451,368	-				-0.5
4,451,368	-		Pf1N1B4_4002	0.32	-0.4
4,451,481	+		Pf1N1B4_4002	0.40	-0.0
4,451,481	+		Pf1N1B4_4002	0.40	+1.0
4,451,482	-		Pf1N1B4_4002	0.40	-1.5
4,451,853	+				+1.8
4,451,854	-		Pf1N1B4_4002	0.64	+1.0
4,452,162	+		Pf1N1B4_4002	0.84	+1.0
4,452,162	+		Pf1N1B4_4002	0.84	-0.2
4,452,162	+		Pf1N1B4_4002	0.84	+0.2
4,452,162	+		Pf1N1B4_4002	0.84	+1.0
4,452,162	+		Pf1N1B4_4002	0.84	+0.0
4,452,162	+		Pf1N1B4_4002	0.84	-0.2
4,452,163	-		Pf1N1B4_4002	0.84	-0.6
4,452,163	-		Pf1N1B4_4002	0.84	+0.7
4,452,163	-		Pf1N1B4_4002	0.84	+0.2
4,452,163	-		Pf1N1B4_4002	0.84	-1.4
4,452,179	+		Pf1N1B4_4002	0.85	+0.3
4,452,186	+		Pf1N1B4_4002	0.86	+1.6
4,452,186	+		Pf1N1B4_4002	0.86	+0.4
4,452,279	+				+0.9
4,452,279	+				+0.9
4,452,279	+				-1.8
4,452,279	+				-0.1
4,452,279	+				-1.0
4,452,300	+				-0.5
4,452,301	-				-0.1
4,453,326	-				+0.0