Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_395 and Pf1N1B4_396 overlap by 4 nucleotides Pf1N1B4_396 and Pf1N1B4_397 are separated by 60 nucleotides
Pf1N1B4_395 - Alcohol dehydrogenase (EC 1.1.1.1), at 464,129 to 465,181
_395
Pf1N1B4_396 - D-galactonate regulator, IclR family, at 465,178 to 465,981
_396
Pf1N1B4_397 - Hexuronate transporter, at 466,042 to 467,352
_397
Position (kb) 465
466 Strain fitness (log2 ratio) -2
-1
0
1
2 at 464.297 kb on + strand, within Pf1N1B4_395 at 464.298 kb on - strand, within Pf1N1B4_395 at 464.356 kb on + strand, within Pf1N1B4_395 at 464.359 kb on + strand at 464.359 kb on + strand, within Pf1N1B4_395 at 464.359 kb on + strand, within Pf1N1B4_395 at 464.359 kb on + strand, within Pf1N1B4_395 at 464.360 kb on - strand, within Pf1N1B4_395 at 464.360 kb on - strand, within Pf1N1B4_395 at 464.360 kb on - strand, within Pf1N1B4_395 at 464.448 kb on - strand, within Pf1N1B4_395 at 464.474 kb on + strand, within Pf1N1B4_395 at 464.474 kb on + strand, within Pf1N1B4_395 at 464.475 kb on - strand, within Pf1N1B4_395 at 464.475 kb on - strand, within Pf1N1B4_395 at 464.621 kb on + strand, within Pf1N1B4_395 at 464.622 kb on - strand, within Pf1N1B4_395 at 464.639 kb on + strand, within Pf1N1B4_395 at 464.640 kb on - strand, within Pf1N1B4_395 at 464.919 kb on + strand at 464.919 kb on + strand, within Pf1N1B4_395 at 464.919 kb on + strand, within Pf1N1B4_395 at 464.920 kb on - strand, within Pf1N1B4_395 at 464.920 kb on - strand, within Pf1N1B4_395 at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.143 kb on + strand at 465.144 kb on - strand at 465.144 kb on - strand at 465.144 kb on - strand at 465.180 kb on - strand at 465.266 kb on + strand, within Pf1N1B4_396 at 465.266 kb on + strand, within Pf1N1B4_396 at 465.266 kb on + strand, within Pf1N1B4_396 at 465.267 kb on - strand, within Pf1N1B4_396 at 465.267 kb on - strand, within Pf1N1B4_396 at 465.393 kb on - strand, within Pf1N1B4_396 at 465.393 kb on - strand, within Pf1N1B4_396 at 465.486 kb on - strand, within Pf1N1B4_396 at 465.617 kb on + strand, within Pf1N1B4_396 at 465.618 kb on - strand, within Pf1N1B4_396 at 465.767 kb on + strand, within Pf1N1B4_396 at 465.794 kb on + strand, within Pf1N1B4_396 at 465.794 kb on + strand, within Pf1N1B4_396 at 465.795 kb on - strand, within Pf1N1B4_396 at 465.835 kb on - strand, within Pf1N1B4_396 at 466.021 kb on - strand at 466.021 kb on - strand at 466.044 kb on - strand at 466.054 kb on - strand at 466.082 kb on + strand at 466.083 kb on - strand at 466.116 kb on + strand at 466.116 kb on + strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.123 kb on - strand at 466.179 kb on + strand, within Pf1N1B4_397 at 466.541 kb on + strand, within Pf1N1B4_397 at 466.542 kb on - strand, within Pf1N1B4_397 at 466.542 kb on - strand, within Pf1N1B4_397 at 466.601 kb on + strand, within Pf1N1B4_397 at 466.601 kb on + strand, within Pf1N1B4_397 at 466.602 kb on - strand, within Pf1N1B4_397 at 466.659 kb on + strand, within Pf1N1B4_397 at 466.660 kb on - strand, within Pf1N1B4_397 at 466.904 kb on + strand, within Pf1N1B4_397 at 466.904 kb on + strand, within Pf1N1B4_397 at 466.904 kb on + strand, within Pf1N1B4_397 at 466.904 kb on + strand, within Pf1N1B4_397 at 466.905 kb on - strand, within Pf1N1B4_397 at 466.905 kb on - strand, within Pf1N1B4_397 at 466.974 kb on - strand, within Pf1N1B4_397
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 remove 464,297 + Pf1N1B4_395 0.16 -0.6 464,298 - Pf1N1B4_395 0.16 -1.6 464,356 + Pf1N1B4_395 0.22 -0.1 464,359 + +0.2 464,359 + Pf1N1B4_395 0.22 +0.9 464,359 + Pf1N1B4_395 0.22 -0.5 464,359 + Pf1N1B4_395 0.22 -0.6 464,360 - Pf1N1B4_395 0.22 -1.3 464,360 - Pf1N1B4_395 0.22 +2.1 464,360 - Pf1N1B4_395 0.22 +0.3 464,448 - Pf1N1B4_395 0.30 -1.6 464,474 + Pf1N1B4_395 0.33 -0.9 464,474 + Pf1N1B4_395 0.33 -2.5 464,475 - Pf1N1B4_395 0.33 -0.6 464,475 - Pf1N1B4_395 0.33 +1.8 464,621 + Pf1N1B4_395 0.47 +0.5 464,622 - Pf1N1B4_395 0.47 -0.1 464,639 + Pf1N1B4_395 0.48 +1.1 464,640 - Pf1N1B4_395 0.49 +0.5 464,919 + +1.7 464,919 + Pf1N1B4_395 0.75 -1.1 464,919 + Pf1N1B4_395 0.75 +2.5 464,920 - Pf1N1B4_395 0.75 -0.7 464,920 - Pf1N1B4_395 0.75 -0.1 465,113 + -0.5 465,113 + -1.0 465,113 + +0.9 465,113 + -2.3 465,113 + -1.3 465,113 + +0.2 465,114 - +0.8 465,114 - -1.3 465,114 - +2.3 465,114 - +0.2 465,114 - -0.4 465,114 - +0.3 465,114 - -0.5 465,143 + +0.5 465,144 - -1.6 465,144 - -0.6 465,144 - -0.1 465,180 - -0.3 465,266 + Pf1N1B4_396 0.11 -1.2 465,266 + Pf1N1B4_396 0.11 -1.1 465,266 + Pf1N1B4_396 0.11 -0.5 465,267 - Pf1N1B4_396 0.11 -0.8 465,267 - Pf1N1B4_396 0.11 -1.4 465,393 - Pf1N1B4_396 0.27 -0.5 465,393 - Pf1N1B4_396 0.27 -1.1 465,486 - Pf1N1B4_396 0.38 +0.1 465,617 + Pf1N1B4_396 0.55 +0.9 465,618 - Pf1N1B4_396 0.55 -1.3 465,767 + Pf1N1B4_396 0.73 -1.8 465,794 + Pf1N1B4_396 0.77 -1.6 465,794 + Pf1N1B4_396 0.77 -1.1 465,795 - Pf1N1B4_396 0.77 -1.3 465,835 - Pf1N1B4_396 0.82 -0.5 466,021 - +0.7 466,021 - -1.9 466,044 - +1.1 466,054 - +0.0 466,082 + +0.5 466,083 - -2.2 466,116 + +0.5 466,116 + +0.3 466,117 - -0.4 466,117 - -0.3 466,117 - +0.3 466,117 - +0.5 466,117 - +0.9 466,117 - +0.5 466,118 + +0.3 466,118 + +0.1 466,118 + -1.3 466,118 + -0.9 466,118 + -0.5 466,119 - -0.1 466,119 - -0.5 466,119 - +0.2 466,119 - -1.1 466,119 - -0.3 466,119 - +0.3 466,119 - -0.3 466,119 - -1.6 466,119 - -1.1 466,119 - +1.2 466,119 - +0.5 466,119 - -0.4 466,119 - +0.5 466,119 - -0.5 466,119 - -0.4 466,119 - -0.6 466,119 - -1.1 466,119 - -0.2 466,119 - -0.1 466,119 - -0.1 466,119 - -0.5 466,119 - -2.1 466,123 - -0.6 466,179 + Pf1N1B4_397 0.10 -0.6 466,541 + Pf1N1B4_397 0.38 +0.1 466,542 - Pf1N1B4_397 0.38 -1.5 466,542 - Pf1N1B4_397 0.38 -1.9 466,601 + Pf1N1B4_397 0.43 -0.3 466,601 + Pf1N1B4_397 0.43 -1.3 466,602 - Pf1N1B4_397 0.43 -0.5 466,659 + Pf1N1B4_397 0.47 -0.9 466,660 - Pf1N1B4_397 0.47 -0.5 466,904 + Pf1N1B4_397 0.66 -0.5 466,904 + Pf1N1B4_397 0.66 -0.5 466,904 + Pf1N1B4_397 0.66 +0.6 466,904 + Pf1N1B4_397 0.66 +1.5 466,905 - Pf1N1B4_397 0.66 +1.5 466,905 - Pf1N1B4_397 0.66 -0.4 466,974 - Pf1N1B4_397 0.71 -1.1
Or see this region's nucleotide sequence