Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_379

Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_376 and Pf1N1B4_377 are separated by 211 nucleotidesPf1N1B4_377 and Pf1N1B4_378 are separated by 5 nucleotidesPf1N1B4_378 and Pf1N1B4_379 are separated by 153 nucleotidesPf1N1B4_379 and Pf1N1B4_380 are separated by 64 nucleotides Pf1N1B4_376 - Deblocking aminopeptidase (EC 3.4.11.-) @ Cyanophycinase 2 (EC 3.4.15.6), at 447,403 to 448,593 _376 Pf1N1B4_377 - FIG00953242: hypothetical protein, at 448,805 to 449,032 _377 Pf1N1B4_378 - Carbon storage regulator, at 449,038 to 449,223 _378 Pf1N1B4_379 - Protein of unknown function UPF0060, at 449,377 to 449,709 _379 Pf1N1B4_380 - Oxidoreductase, short chain dehydrogenase/reductase family, at 449,774 to 450,589 _380 Position (kb) 449 450Strain fitness (log2 ratio) -2 -1 0 1 2at 448.438 kb on + strand, within Pf1N1B4_376at 448.438 kb on + strand, within Pf1N1B4_376at 448.439 kb on - strand, within Pf1N1B4_376at 448.439 kb on - strand, within Pf1N1B4_376at 448.439 kb on - strand, within Pf1N1B4_376at 448.439 kb on - strand, within Pf1N1B4_376at 448.747 kb on - strandat 448.762 kb on - strandat 448.804 kb on + strandat 448.804 kb on + strandat 449.082 kb on - strand, within Pf1N1B4_378at 449.082 kb on - strand, within Pf1N1B4_378at 449.082 kb on - strand, within Pf1N1B4_378at 449.082 kb on - strand, within Pf1N1B4_378at 449.098 kb on + strand, within Pf1N1B4_378at 449.098 kb on + strand, within Pf1N1B4_378at 449.098 kb on + strand, within Pf1N1B4_378at 449.099 kb on - strand, within Pf1N1B4_378at 449.099 kb on - strand, within Pf1N1B4_378at 449.099 kb on - strand, within Pf1N1B4_378at 449.099 kb on - strand, within Pf1N1B4_378at 449.099 kb on - strand, within Pf1N1B4_378at 449.216 kb on - strandat 449.312 kb on - strandat 449.312 kb on - strandat 449.358 kb on + strandat 449.359 kb on - strandat 449.510 kb on + strand, within Pf1N1B4_379at 449.510 kb on + strand, within Pf1N1B4_379at 449.510 kb on + strand, within Pf1N1B4_379at 449.511 kb on - strand, within Pf1N1B4_379at 449.511 kb on - strand, within Pf1N1B4_379at 449.511 kb on - strand, within Pf1N1B4_379at 449.511 kb on - strand, within Pf1N1B4_379at 449.511 kb on - strand, within Pf1N1B4_379at 449.511 kb on - strand, within Pf1N1B4_379at 449.522 kb on + strand, within Pf1N1B4_379at 449.523 kb on - strand, within Pf1N1B4_379at 449.625 kb on + strandat 449.625 kb on + strand, within Pf1N1B4_379at 449.626 kb on - strand, within Pf1N1B4_379at 449.699 kb on + strandat 449.699 kb on + strandat 449.699 kb on + strandat 449.699 kb on + strandat 449.699 kb on + strandat 449.700 kb on - strandat 449.700 kb on - strandat 449.700 kb on - strandat 449.700 kb on - strandat 449.700 kb on - strandat 449.700 kb on - strandat 449.700 kb on - strandat 449.700 kb on - strandat 449.835 kb on + strandat 449.836 kb on - strandat 449.836 kb on - strandat 449.928 kb on + strand, within Pf1N1B4_380at 449.934 kb on + strand, within Pf1N1B4_380at 449.934 kb on + strand, within Pf1N1B4_380at 449.934 kb on + strand, within Pf1N1B4_380at 449.935 kb on - strand, within Pf1N1B4_380at 449.935 kb on - strand, within Pf1N1B4_380at 449.935 kb on - strandat 450.141 kb on + strand, within Pf1N1B4_380at 450.141 kb on + strand, within Pf1N1B4_380at 450.141 kb on + strand, within Pf1N1B4_380at 450.141 kb on + strand, within Pf1N1B4_380at 450.142 kb on - strand, within Pf1N1B4_380at 450.142 kb on - strand, within Pf1N1B4_380at 450.142 kb on - strandat 450.142 kb on - strand, within Pf1N1B4_380at 450.142 kb on - strand, within Pf1N1B4_380at 450.142 kb on - strand, within Pf1N1B4_380at 450.641 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
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448,438 + Pf1N1B4_376 0.87 +0.6
448,438 + Pf1N1B4_376 0.87 +0.2
448,439 - Pf1N1B4_376 0.87 -1.1
448,439 - Pf1N1B4_376 0.87 -0.1
448,439 - Pf1N1B4_376 0.87 +0.5
448,439 - Pf1N1B4_376 0.87 -0.7
448,747 - +0.9
448,762 - -0.6
448,804 + -0.4
448,804 + +0.2
449,082 - Pf1N1B4_378 0.24 -2.0
449,082 - Pf1N1B4_378 0.24 +1.2
449,082 - Pf1N1B4_378 0.24 -0.5
449,082 - Pf1N1B4_378 0.24 +0.1
449,098 + Pf1N1B4_378 0.32 -1.6
449,098 + Pf1N1B4_378 0.32 -0.2
449,098 + Pf1N1B4_378 0.32 -2.1
449,099 - Pf1N1B4_378 0.33 -1.3
449,099 - Pf1N1B4_378 0.33 -1.7
449,099 - Pf1N1B4_378 0.33 -0.6
449,099 - Pf1N1B4_378 0.33 -0.7
449,099 - Pf1N1B4_378 0.33 +0.5
449,216 - -0.1
449,312 - +0.1
449,312 - +0.4
449,358 + -0.6
449,359 - -0.1
449,510 + Pf1N1B4_379 0.40 -1.1
449,510 + Pf1N1B4_379 0.40 -0.1
449,510 + Pf1N1B4_379 0.40 -0.1
449,511 - Pf1N1B4_379 0.40 -0.1
449,511 - Pf1N1B4_379 0.40 -0.9
449,511 - Pf1N1B4_379 0.40 -1.5
449,511 - Pf1N1B4_379 0.40 -1.1
449,511 - Pf1N1B4_379 0.40 +0.2
449,511 - Pf1N1B4_379 0.40 -0.5
449,522 + Pf1N1B4_379 0.44 -0.8
449,523 - Pf1N1B4_379 0.44 -1.0
449,625 + -1.3
449,625 + Pf1N1B4_379 0.74 -1.5
449,626 - Pf1N1B4_379 0.75 +0.1
449,699 + -0.2
449,699 + +0.1
449,699 + +0.5
449,699 + -0.2
449,699 + -0.2
449,700 - +0.2
449,700 - +0.2
449,700 - +1.3
449,700 - -2.0
449,700 - +0.6
449,700 - +0.5
449,700 - -1.4
449,700 - +0.1
449,835 + -0.5
449,836 - +0.5
449,836 - -1.1
449,928 + Pf1N1B4_380 0.19 +1.2
449,934 + Pf1N1B4_380 0.20 -0.4
449,934 + Pf1N1B4_380 0.20 +0.1
449,934 + Pf1N1B4_380 0.20 +1.9
449,935 - Pf1N1B4_380 0.20 -0.7
449,935 - Pf1N1B4_380 0.20 -0.3
449,935 - -0.5
450,141 + Pf1N1B4_380 0.45 +0.8
450,141 + Pf1N1B4_380 0.45 -0.3
450,141 + Pf1N1B4_380 0.45 -2.2
450,141 + Pf1N1B4_380 0.45 -1.0
450,142 - Pf1N1B4_380 0.45 +0.8
450,142 - Pf1N1B4_380 0.45 +2.1
450,142 - +1.2
450,142 - Pf1N1B4_380 0.45 -1.3
450,142 - Pf1N1B4_380 0.45 -0.5
450,142 - Pf1N1B4_380 0.45 -1.3
450,641 - +0.4

Or see this region's nucleotide sequence